For histological analysis, we utilized both paraffin sections and frozen sections. For both secti... more For histological analysis, we utilized both paraffin sections and frozen sections. For both section techniques, control and mutant littermates at P22, P90, 6-12 months and 12-20 months of age were perfused with 4 % paraformaldehyde (PFA). For paraffin sections, sciatic nerves were dissected, post-fixed in PFA overnight at 4°C, and processed for paraffin embedding. Sciatic nerves were sectioned longitudinally at 5 µm thickness. For frozen sections, sciatic nerves were dissected, post-fixed in PFA for 2 hours at 4°C and transferred to 30 % sucrose overnight at 4°C. Tissue was embedded in using O.C.T. compound (Tissue-Tek) and frozen in crushed dry ice. Sciatic nerves were sectioned longitudinally at 6 or 14 µm thickness. Histology and Tumor Grading Two to three paraffin sectioned slides were stained with hematoxylin/eosin (H&E). Diagnosis for each sciatic nerve lesion was generated based upon histology from H&E stained sections under the light microscopy. The penetrance and frequency of neurofibromas or pre-neoplastic lesions in control and mutant mice was quantified based upon sciatic nerve diagnoses. Quantification of the number of cells per surface area (mm2) in P22 and P90 control and mutant sciatic nerves was performed using ImageJ on digital images of H&E stained sections. For PCR analysis, tissues were dissected in ice-cold PBS and digested with proteinase K as described (Zhu et al., 2005).
Molecular & cellular proteomics : MCP, Jan 27, 2017
Multigene families encoding diverse secreted peptide hormones play important roles in plant devel... more Multigene families encoding diverse secreted peptide hormones play important roles in plant development. A need exists to efficiently elucidate the structures and post-translational modifications of these difficult-to-isolate peptide hormones in planta so that their biological functions can be determined. A mass spectrometry and bioinformatics approach was developed to comprehensively analyse the secreted peptidome of Medicago hairy root cultures and xylem sap. We identified 759 spectra corresponding to the secreted products of twelve peptide hormones including four CEP (C-TERMINALLY ENCODED PEPTIDE), two CLE (CLV3/ENDOSPERM SURROUNDING REGION RELATED) and six XAP (XYLEM SAP ASSOCIATED PEPTIDE) peptides. The MtCEP1, MtCEP2, MtCEP5 and MtCEP8 peptides identified differed in post-translational-modifications. Most were hydroxylated at conserved proline residues but some MtCEP1 derivatives were triarabinosylated. In addition, many CEP peptides possessed unexpected N- and C-terminal exte...
Efectos de la salinidad del suelo en el crecimiento, estado hídrico y acumulación de nutrientes e... more Efectos de la salinidad del suelo en el crecimiento, estado hídrico y acumulación de nutrientes en semillas de Aegiceras corniculatum (Myrsinaceae). ... Se realizaron experimentos en invernadero para valorar los efectos de la salinidad del suelo en la emergencia, crecimiento, estado ...
For histological analysis, we utilized both paraffin sections and frozen sections. For both secti... more For histological analysis, we utilized both paraffin sections and frozen sections. For both section techniques, control and mutant littermates at P22, P90, 6-12 months and 12-20 months of age were perfused with 4 % paraformaldehyde (PFA). For paraffin sections, sciatic nerves were dissected, post-fixed in PFA overnight at 4°C, and processed for paraffin embedding. Sciatic nerves were sectioned longitudinally at 5 µm thickness. For frozen sections, sciatic nerves were dissected, post-fixed in PFA for 2 hours at 4°C and transferred to 30 % sucrose overnight at 4°C. Tissue was embedded in using O.C.T. compound (Tissue-Tek) and frozen in crushed dry ice. Sciatic nerves were sectioned longitudinally at 6 or 14 µm thickness. Histology and Tumor Grading Two to three paraffin sectioned slides were stained with hematoxylin/eosin (H&E). Diagnosis for each sciatic nerve lesion was generated based upon histology from H&E stained sections under the light microscopy. The penetrance and frequency of neurofibromas or pre-neoplastic lesions in control and mutant mice was quantified based upon sciatic nerve diagnoses. Quantification of the number of cells per surface area (mm2) in P22 and P90 control and mutant sciatic nerves was performed using ImageJ on digital images of H&E stained sections. For PCR analysis, tissues were dissected in ice-cold PBS and digested with proteinase K as described (Zhu et al., 2005).
Molecular & cellular proteomics : MCP, Jan 27, 2017
Multigene families encoding diverse secreted peptide hormones play important roles in plant devel... more Multigene families encoding diverse secreted peptide hormones play important roles in plant development. A need exists to efficiently elucidate the structures and post-translational modifications of these difficult-to-isolate peptide hormones in planta so that their biological functions can be determined. A mass spectrometry and bioinformatics approach was developed to comprehensively analyse the secreted peptidome of Medicago hairy root cultures and xylem sap. We identified 759 spectra corresponding to the secreted products of twelve peptide hormones including four CEP (C-TERMINALLY ENCODED PEPTIDE), two CLE (CLV3/ENDOSPERM SURROUNDING REGION RELATED) and six XAP (XYLEM SAP ASSOCIATED PEPTIDE) peptides. The MtCEP1, MtCEP2, MtCEP5 and MtCEP8 peptides identified differed in post-translational-modifications. Most were hydroxylated at conserved proline residues but some MtCEP1 derivatives were triarabinosylated. In addition, many CEP peptides possessed unexpected N- and C-terminal exte...
Efectos de la salinidad del suelo en el crecimiento, estado hídrico y acumulación de nutrientes e... more Efectos de la salinidad del suelo en el crecimiento, estado hídrico y acumulación de nutrientes en semillas de Aegiceras corniculatum (Myrsinaceae). ... Se realizaron experimentos en invernadero para valorar los efectos de la salinidad del suelo en la emergencia, crecimiento, estado ...
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