The OpenSHMEM Analyzer is a compiler-based tool that can help users detect errors and provide use... more The OpenSHMEM Analyzer is a compiler-based tool that can help users detect errors and provide useful analyses about their OpenSHMEM applications. The tool is built on top of the OpenUH compiler (a branch of Open64 compiler) and presents OpenSHMEM information as feedback to the user. Some of the analyses it provides include checks for correct usage of symmetric variables in OpenSHMEM calls, out-of-bounds checks for symmetric data, checks for the correct initialization of pointers to symmetric data, and symmetric data alias information.
Aunque es claro que existe un consenso sobre las principales categorias de la teoria de las repre... more Aunque es claro que existe un consenso sobre las principales categorias de la teoria de las representaciones sociales (RS), (Moscovici, 1961; Jodelet, 2000), alli se encuentra latente un conjunto amplio de discusiones que pasan inadvertidas aun entre investigadores que le usan. En el presente articulo se proponen tres debates que le subyacen; la relacion entre la RS y le ideologia, su naturaleza epistemologica y el papel de la subjetividad dentro de su constitucion. Se pretende crear un espacio en donde se enriquezca esta teoria y no simplemente se reduzca a su aplicacion instrumentalizada.
In Mexico, around six million people have some kind of disability, of which neuromuscular disease... more In Mexico, around six million people have some kind of disability, of which neuromuscular diseases remain one of the most common causes of inability and death. Neuromuscular disorders are rare conditions that affect the muscles, the neuromuscular junction, the peripheral motor nerves and the motor neurons. Their clinical and genetic variability impact in an ef fective diagnosis; therefore, many cases are identified belatedly or not diagnosed at all, due to lack economic resources or the absence of specialized study centers for neuromuscular diseases. In this article, we offer a general description of the most frequent neuromuscular
ARticLe ADDenDumAddendum to: Hernandez-Hernandez O, Guiraud-Dogan C, Sicot G, Huguet A, Luilier S... more ARticLe ADDenDumAddendum to: Hernandez-Hernandez O, Guiraud-Dogan C, Sicot G, Huguet A, Luilier S, Steidl E, et al. Myotonic dystrophy CTG expansion affects synaptic vesicle proteins, neurotransmis-sion and mouse behaviour. Brain 2013; 136:957-70; PMID:23404338; http://dx.doi.org/10.1093/brain/aws367
Myotonic dystrophy type 1 (DM1), the most frequent inherited muscular dystrophy in adults, is cau... more Myotonic dystrophy type 1 (DM1), the most frequent inherited muscular dystrophy in adults, is caused by the CTG repeat expansion in the 3′UTR of the DMPK gene. Mutant DMPK RNA accumulates in nuclear foci altering diverse cellular functions including alternative splicing regulation. DM1 is a multisystemic condition, with debilitating central nervous system alterations. Although a defective neuroglia communication has been described as a contributor of the brain pathology in DM1, the specific cellular and molecular events potentially affected in glia cells have not been totally recognized. Thus, to study the effects of DM1 mutation on glial physiology, in this work, we have established an inducible DM1 model derived from the MIO-M1 cell line expressing 648 CUG repeats. This new model recreated the molecular hallmarks of DM1 elicited by a toxic RNA gain-of-function mechanism: accumulation of RNA foci colocalized with MBNL proteins and dysregulation of alternative splicing. By applying ...
Abstract An efficient separation of the isomeric thioether metabolites of styrene oxide was achie... more Abstract An efficient separation of the isomeric thioether metabolites of styrene oxide was achieved under reversed-phase conditions. The column was eluted isocratically with 15% methanol in buffered solutions of phosphoric acid-tris-hydroxymethylaminomethane. The thioether conjugates were separated by class, and the order of elution was cysteine, cysteinylglycine, glutathione, and N-acetylcysteine. The effect of pH and buffer salt concentration on the HPLC separation was examined. Optimal conditions for a separation were either found at low pH (pH 3 or 4) or neutral pH, both at a high buffer salt concentration (75mM). The positional isomers and stereoisomers comprising each amino acid conjugate sample were separated into two peaks. The variations in k1 and α observed with changes in pH were interpreted as reflecting the degree of interaction of the ionizable groups in the amino acid residue and the hydrophobic portion of the molecule. This interaction was found to be strongly influenced by the relative s...
Se presenta un nuevo asentamiento de los denominados “campos de hoyos” de la Edad del Bronce del ... more Se presenta un nuevo asentamiento de los denominados “campos de hoyos” de la Edad del Bronce del interior peninsular. Aun cuando las estructuras negativas aparecen muy arrasadas, es posible identificar una diversa tipología que confirma actividades vinculadas a la explotación de recursos naturales por un grupo que utilizaba cerámicas típicas de Cogotas I. La comparación tipológica y decorativa de los materiales cerámicos reclama su evolución desde grupos de amplio arraigo en el territorio, cuestionando la vinculación de la técnica excisa a influencias foráneas, rastreando su presencia desde la fase formativa del grupo, junto con la incisión y el boquique, singularmente en el oriente de la Submeseta Norte.
Spinocerebellar ataxia type 7 is a neurodegenerative inherited disease caused by a CAG expansion ... more Spinocerebellar ataxia type 7 is a neurodegenerative inherited disease caused by a CAG expansion in the coding region of the ATXN7 gene, which results in the synthesis of polyglutamine-containing ataxin-7. Expression of mutant ataxin-7 disturbs different cell processes, including transcriptional regulation, protein conformation and clearance, autophagy, and glutamate transport; however, mechanisms underlying neurodegeneration in SCA7 are still unknown. Implication of oxidative stress in the pathogenesis of various neurodegenerative diseases, including polyglutamine disorders, has recently emerged. We perform a cross-sectional study to determine for the first time pheripheral levels of different oxidative stress markers in 29 SCA7 patients and 28 age- and sex-matched healthy subjects. Patients with SCA7 exhibit oxidative damage to lipids (high levels of lipid hydroperoxides and malondialdehyde) and proteins (elevated levels of advanced oxidation protein products and protein carbonyls...
Burns : journal of the International Society for Burn Injuries, Jan 22, 2018
Sepsis is a life-threatening organ-dysfunction condition caused by a dysregulated response to an ... more Sepsis is a life-threatening organ-dysfunction condition caused by a dysregulated response to an infectious condition that can cause complications in patients with major trauma. Burns are one of the most destructive forms of trauma; despite the improvements in medical care, infections remain an important cause of burn injury-related mortality and morbidity, and complicated sepsis predisposes patients to diverse complications such as organ failure, lengthening of hospital stays, and increased costs. Accurate diagnosis and early treatment of sepsis may have a beneficial impact on clinical outcome of burn-injured patients. In this review, we offer a comprehensive description of the current and traditional markers used as indicative of sepsis in burned patients. However, although these are markers of the inflammatory post-burn response, they usually fail to predict sepsis in severely burned patients due to that they do not reflect the severity of the infection. Identification and measur...
Spinocerebellar ataxia type 7 (SCA7) is a rare neurogenetic disorder caused by highly unstable CA... more Spinocerebellar ataxia type 7 (SCA7) is a rare neurogenetic disorder caused by highly unstable CAG repeat expansion mutation in coding region of SCA7. We aimed to understand the effect of diverse ATXN7 cis-element in correlation with CAG expansion mutation of SCA7. We initially performed an analysis to identify the haplotype background of CAG expanded alleles using eight bi-allelic single nucleotide polymorphisms (SNPs) flanking an ATXN7-CAG expansion in 32 individuals from nine unrelated Indian SCA7 families and 88 healthy controls. Subsequent validation of the findings was performed in 89 ATXN7-CAG mutation carriers and in 119 unrelated healthy controls of Mexican ancestry. The haplotype analyses showed a shared haplotype background and C allele of SNP rs6798742 (approximately 6 kb from the 3'-end of CAG repeats) is in complete association with expanded, premutation, intermediate, and the majority of large normal (≥12) CAG allele. The C allele (ancestral/chimp allele) associat...
The arginine-vasopressin (AVP)-containing hypothalamic magnocellular neurosecretory neurons (VPMN... more The arginine-vasopressin (AVP)-containing hypothalamic magnocellular neurosecretory neurons (VPMNNs) are known for their role in hydro-electrolytic balance control via their projections to the neurohypophysis. Recently, projections from these same neurons to hippocampus, habenula and other brain regions in which vasopressin infusion modulates contingent social and emotionally-affected behaviors, have been reported. Here, we present evidence that VPMNN collaterals also project to the amygdaloid complex, and establish synaptic connections with neurons in central amygdala (CeA). The density of AVP innervation in amygdala was substantially increased in adult rats that had experienced neonatal maternal separation (MS), consistent with our previous observations that MS enhances VPMNN number in the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus. In the CeA, V1a AVP receptor mRNA was only observed in GABAergic neurons, demonstrated by complete co-localization of V1a t...
The OpenSHMEM Analyzer is a compiler-based tool that can help users detect errors and provide use... more The OpenSHMEM Analyzer is a compiler-based tool that can help users detect errors and provide useful analyses about their OpenSHMEM applications. The tool is built on top of the OpenUH compiler (a branch of Open64 compiler) and presents OpenSHMEM information as feedback to the user. Some of the analyses it provides include checks for correct usage of symmetric variables in OpenSHMEM calls, out-of-bounds checks for symmetric data, checks for the correct initialization of pointers to symmetric data, and symmetric data alias information.
Aunque es claro que existe un consenso sobre las principales categorias de la teoria de las repre... more Aunque es claro que existe un consenso sobre las principales categorias de la teoria de las representaciones sociales (RS), (Moscovici, 1961; Jodelet, 2000), alli se encuentra latente un conjunto amplio de discusiones que pasan inadvertidas aun entre investigadores que le usan. En el presente articulo se proponen tres debates que le subyacen; la relacion entre la RS y le ideologia, su naturaleza epistemologica y el papel de la subjetividad dentro de su constitucion. Se pretende crear un espacio en donde se enriquezca esta teoria y no simplemente se reduzca a su aplicacion instrumentalizada.
In Mexico, around six million people have some kind of disability, of which neuromuscular disease... more In Mexico, around six million people have some kind of disability, of which neuromuscular diseases remain one of the most common causes of inability and death. Neuromuscular disorders are rare conditions that affect the muscles, the neuromuscular junction, the peripheral motor nerves and the motor neurons. Their clinical and genetic variability impact in an ef fective diagnosis; therefore, many cases are identified belatedly or not diagnosed at all, due to lack economic resources or the absence of specialized study centers for neuromuscular diseases. In this article, we offer a general description of the most frequent neuromuscular
ARticLe ADDenDumAddendum to: Hernandez-Hernandez O, Guiraud-Dogan C, Sicot G, Huguet A, Luilier S... more ARticLe ADDenDumAddendum to: Hernandez-Hernandez O, Guiraud-Dogan C, Sicot G, Huguet A, Luilier S, Steidl E, et al. Myotonic dystrophy CTG expansion affects synaptic vesicle proteins, neurotransmis-sion and mouse behaviour. Brain 2013; 136:957-70; PMID:23404338; http://dx.doi.org/10.1093/brain/aws367
Myotonic dystrophy type 1 (DM1), the most frequent inherited muscular dystrophy in adults, is cau... more Myotonic dystrophy type 1 (DM1), the most frequent inherited muscular dystrophy in adults, is caused by the CTG repeat expansion in the 3′UTR of the DMPK gene. Mutant DMPK RNA accumulates in nuclear foci altering diverse cellular functions including alternative splicing regulation. DM1 is a multisystemic condition, with debilitating central nervous system alterations. Although a defective neuroglia communication has been described as a contributor of the brain pathology in DM1, the specific cellular and molecular events potentially affected in glia cells have not been totally recognized. Thus, to study the effects of DM1 mutation on glial physiology, in this work, we have established an inducible DM1 model derived from the MIO-M1 cell line expressing 648 CUG repeats. This new model recreated the molecular hallmarks of DM1 elicited by a toxic RNA gain-of-function mechanism: accumulation of RNA foci colocalized with MBNL proteins and dysregulation of alternative splicing. By applying ...
Abstract An efficient separation of the isomeric thioether metabolites of styrene oxide was achie... more Abstract An efficient separation of the isomeric thioether metabolites of styrene oxide was achieved under reversed-phase conditions. The column was eluted isocratically with 15% methanol in buffered solutions of phosphoric acid-tris-hydroxymethylaminomethane. The thioether conjugates were separated by class, and the order of elution was cysteine, cysteinylglycine, glutathione, and N-acetylcysteine. The effect of pH and buffer salt concentration on the HPLC separation was examined. Optimal conditions for a separation were either found at low pH (pH 3 or 4) or neutral pH, both at a high buffer salt concentration (75mM). The positional isomers and stereoisomers comprising each amino acid conjugate sample were separated into two peaks. The variations in k1 and α observed with changes in pH were interpreted as reflecting the degree of interaction of the ionizable groups in the amino acid residue and the hydrophobic portion of the molecule. This interaction was found to be strongly influenced by the relative s...
Se presenta un nuevo asentamiento de los denominados “campos de hoyos” de la Edad del Bronce del ... more Se presenta un nuevo asentamiento de los denominados “campos de hoyos” de la Edad del Bronce del interior peninsular. Aun cuando las estructuras negativas aparecen muy arrasadas, es posible identificar una diversa tipología que confirma actividades vinculadas a la explotación de recursos naturales por un grupo que utilizaba cerámicas típicas de Cogotas I. La comparación tipológica y decorativa de los materiales cerámicos reclama su evolución desde grupos de amplio arraigo en el territorio, cuestionando la vinculación de la técnica excisa a influencias foráneas, rastreando su presencia desde la fase formativa del grupo, junto con la incisión y el boquique, singularmente en el oriente de la Submeseta Norte.
Spinocerebellar ataxia type 7 is a neurodegenerative inherited disease caused by a CAG expansion ... more Spinocerebellar ataxia type 7 is a neurodegenerative inherited disease caused by a CAG expansion in the coding region of the ATXN7 gene, which results in the synthesis of polyglutamine-containing ataxin-7. Expression of mutant ataxin-7 disturbs different cell processes, including transcriptional regulation, protein conformation and clearance, autophagy, and glutamate transport; however, mechanisms underlying neurodegeneration in SCA7 are still unknown. Implication of oxidative stress in the pathogenesis of various neurodegenerative diseases, including polyglutamine disorders, has recently emerged. We perform a cross-sectional study to determine for the first time pheripheral levels of different oxidative stress markers in 29 SCA7 patients and 28 age- and sex-matched healthy subjects. Patients with SCA7 exhibit oxidative damage to lipids (high levels of lipid hydroperoxides and malondialdehyde) and proteins (elevated levels of advanced oxidation protein products and protein carbonyls...
Burns : journal of the International Society for Burn Injuries, Jan 22, 2018
Sepsis is a life-threatening organ-dysfunction condition caused by a dysregulated response to an ... more Sepsis is a life-threatening organ-dysfunction condition caused by a dysregulated response to an infectious condition that can cause complications in patients with major trauma. Burns are one of the most destructive forms of trauma; despite the improvements in medical care, infections remain an important cause of burn injury-related mortality and morbidity, and complicated sepsis predisposes patients to diverse complications such as organ failure, lengthening of hospital stays, and increased costs. Accurate diagnosis and early treatment of sepsis may have a beneficial impact on clinical outcome of burn-injured patients. In this review, we offer a comprehensive description of the current and traditional markers used as indicative of sepsis in burned patients. However, although these are markers of the inflammatory post-burn response, they usually fail to predict sepsis in severely burned patients due to that they do not reflect the severity of the infection. Identification and measur...
Spinocerebellar ataxia type 7 (SCA7) is a rare neurogenetic disorder caused by highly unstable CA... more Spinocerebellar ataxia type 7 (SCA7) is a rare neurogenetic disorder caused by highly unstable CAG repeat expansion mutation in coding region of SCA7. We aimed to understand the effect of diverse ATXN7 cis-element in correlation with CAG expansion mutation of SCA7. We initially performed an analysis to identify the haplotype background of CAG expanded alleles using eight bi-allelic single nucleotide polymorphisms (SNPs) flanking an ATXN7-CAG expansion in 32 individuals from nine unrelated Indian SCA7 families and 88 healthy controls. Subsequent validation of the findings was performed in 89 ATXN7-CAG mutation carriers and in 119 unrelated healthy controls of Mexican ancestry. The haplotype analyses showed a shared haplotype background and C allele of SNP rs6798742 (approximately 6 kb from the 3'-end of CAG repeats) is in complete association with expanded, premutation, intermediate, and the majority of large normal (≥12) CAG allele. The C allele (ancestral/chimp allele) associat...
The arginine-vasopressin (AVP)-containing hypothalamic magnocellular neurosecretory neurons (VPMN... more The arginine-vasopressin (AVP)-containing hypothalamic magnocellular neurosecretory neurons (VPMNNs) are known for their role in hydro-electrolytic balance control via their projections to the neurohypophysis. Recently, projections from these same neurons to hippocampus, habenula and other brain regions in which vasopressin infusion modulates contingent social and emotionally-affected behaviors, have been reported. Here, we present evidence that VPMNN collaterals also project to the amygdaloid complex, and establish synaptic connections with neurons in central amygdala (CeA). The density of AVP innervation in amygdala was substantially increased in adult rats that had experienced neonatal maternal separation (MS), consistent with our previous observations that MS enhances VPMNN number in the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus. In the CeA, V1a AVP receptor mRNA was only observed in GABAergic neurons, demonstrated by complete co-localization of V1a t...
Uploads