The ability of Diplodia pinea to inhibit Armillaria sp., Bjerkandera adusta, Botrytis cinerea, an... more The ability of Diplodia pinea to inhibit Armillaria sp., Bjerkandera adusta, Botrytis cinerea, and Rhizoctonia sp. mycelium growth was analyzed using the double-culture method. Wild-type fungal strains were incubated in a biochemical oxygen demand incubator using potato agar dextrose medium at 24 ± 2 °C for 35 days in darkness. D. pinea significantly inhibited the growth of all fungi species tested (30.75 to 98.37% inhibition) and showed moderate antagonistic activity (antagonistic index, 14.5). Chemical analysis of D. pinea culture broth extracts revealed steroids, triterpenes, and phenolic compounds. Alkaloids were qualitatively detected in the mycelium crude extract. The presence of these compounds may be related to the antagonistic activity observed. The inhibition ability of D. pinea is due to competition with the tested fungi for substrate and space.
There have been few studies on the pharmacological properties of var. , a native Brazilian specie... more There have been few studies on the pharmacological properties of var. , a native Brazilian species popularly known as "fruto-de-pombo." The aim of this study was to investigate the scavenging capacity of emodin, physcion, and the ethanolic crude extract of var. against reactive oxygen and nitrogen species, as well as their role and plausible mechanisms in prompting cell death and changes in AKT phosphorylation after cervical (SiHa and C33A) and oral (HSC-3) squamous cell carcinoma treatments. Emodin was shown to be the best scavenger of NO and O, while all samples were equally effective in HOCl/OCl capture. Emodin, physcion, and the ethanolic extract all exhibited cytotoxic effects on SiHa, C33A, HSC-3, and HaCaT (immortalized human keratinocytes, nontumorigenic cell line), involving mixed cell death (apoptosis and necrosis) independent of the caspase activation pathway. Emodin, physcion, and the ethanolic extract increased intracellular oxidative stress and DNA damage. Em...
Antimicrobial resistance is a critical health problem, and pathogens responsible for common infec... more Antimicrobial resistance is a critical health problem, and pathogens responsible for common infections have developed resistance to antimicrobials, posing a threat to global health and placing a huge burden on health services. During the past two decades, the search for new bioactive agents in nature has become extremely important for promoting health and in the development of more efficient antimicrobials. The genus is one of the largest in the Myrtaceae family, comprising approximately 1000 species from Mexico to Argentina, with a few species distributed in Australia and Africa. species are used in folk medicine, with antidiabetic, antirheumatic, antipyretic, anti-inflammatory, antidiarrheal, antifungal, and antibacterial properties. This study systematically reviews the species to compile the phytochemical composition and antimicrobial effects. In addition, we provide information regarding the traditional uses and cytotoxic activity of species. We conducted a systematic literatur...
Acta Crystallographica Section C Crystal Structure Communications
... OBDULIO G. MIGUEL,a MOACIR G. PIZZOLA"ITI,a ROSENDO A. YUNES,a IVO VENCATO,b... more ... OBDULIO G. MIGUEL,a MOACIR G. PIZZOLA"ITI,a ROSENDO A. YUNES,a IVO VENCATO,b JO,KO B. CALIXTO,c ADAIR RS SANTOS,c ADRIANA CONTINd AND EDUARDO A. MOREIRAd. "Depto. ... C., Guagliardi, A.. Burla, M. C,, Polidori, G. & Camalli. M. (1994). J. Appl. Crxst. ...
Toxicology in vitro : an international journal published in association with BIBRA, Jan 2, 2017
The aim of this study was to investigate the cytotoxic activity of the essential oil from cladode... more The aim of this study was to investigate the cytotoxic activity of the essential oil from cladodes of Bacharis milleflora in relation to Jurkat, Raji and HL-60 cells, as well as exploring the cell mechanisms in order to elucidate how the cytotoxic process occurs. The presence of the following volatile compounds was detected by GC-MS: bicyclogermacrene (12.16%), germacrene D (11.18%), (E)-caryophyllene (9.28%), and α-humulene (8.05%). In general, IC50 values lower than 50μg/mL were obtained for all the tumor cells at 24, 48 and 72h by MTT assay. The decrease in cell DNA content was demonstrated due to the inhibition of the proliferation of Jurkat, Raji and HL-60 cells by B. milleflora essential oil. In particular, Raji cells presented the greatest inhibition of cell proliferation and they were subsequently used to investigate cell death mechanisms. B. milleflora essential oil promoted G0/G1 arrest and also induced cell fragmentation, which was represented by an increase in the sub-G0...
Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with ... more Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, Byrsonima duckeana W. R. Anderson is an Amazonian species almost not studied. Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities of Byrsonima duckeana leaves. Materials and Methods. We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays. Results. All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples. B. duckeana was able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in t...
Evidence-based complementary and alternative medicine : eCAM, 2016
The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically u... more The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of S. larvata were phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract of S. larvata revealed the presence of three flavonoids, drabanemoroside, kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-α-L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compounds p-hydroxybenzoic acid and p-coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed...
Ecotoxicology and environmental safety, Jan 30, 2017
The frequent use of synthetic pesticides to control Aedes aegypti population can lead to environm... more The frequent use of synthetic pesticides to control Aedes aegypti population can lead to environmental and/or human contamination and the emergence of resistant insects. Linalool and methyl cinnamate are presented as an alternative to the synthetic pesticides, since they can exhibit larvicidal, repellent and/or insecticidal activity and are considered safe for use. The aim of this study was to evaluate the larvicidal activity of methyl cinnamate, linalool and methyl cinnamate/linalool in combination (MC-L) (1:4 ratio, respectively) against Aedes aegypti. The in vitro preliminary toxicity through brine shrimp lethality assay and hemolytic activity, and the phytotoxic potential were also investigated to assess the safety of their use as larvicide. Methyl cinnamate showed significant larvicidal activity when compared to linalool (LC50 values of 35.4µg/mL and 275.2µg/mL, respectively) and to MC-L (LC50 138.0µg/mL). Larvae morphological changes subjected to the specified treatments were ...
As plantas são utilizadas por muitos povos como recurso à terapia de doenças, dessa forma, tem se... more As plantas são utilizadas por muitos povos como recurso à terapia de doenças, dessa forma, tem se intensificado as pesquisas por substâncias provindas de plantas e compostos naturais que possam ser utilizadas como fármacos. Os valores de umidade, cinzas totais e os resultados da investigação fitoquímica são essenciais para o controle de qualidade e para a caracterização das espécies vegetais. Temnadenia stellaris, planta relatada de forma endêmica no Brasil, é uma liana pertencente à família Apocynaceae, as plantas desta família são descritas como potenciais fontes de substâncias bioativas devido à importância dada aos metabólitos secundários já encontrados. Tendo em vista os poucos estudos envolvendo a espécie, foram realizados testes para determinar os parâmetros fitoquímicos da planta. O caule de Temnadenia stellaris apresentou umidade de 7,7%, enquanto que as folhas tiveram 11,88% de umidade segundo o ensaio realizado. O caule de Temnadenia stellaris apresentou teor de cinzas de...
Page 1. Revista Lecta, Bragança Paulista, v. 21, n. 1/2, p. 7-13, jan./dez. 2003 7 Fitoterápicos:... more Page 1. Revista Lecta, Bragança Paulista, v. 21, n. 1/2, p. 7-13, jan./dez. 2003 7 Fitoterápicos: uma abordagem farmacotécnica Ana Cristina Oltramari Toledo1 Lilian Lúcio Hirata1 Marilene da Cruz M. Buffon 2, 3 Marilis Dallarmi Miguel 4 Obdulio Gomes Miguel 4 ...
The ability of Diplodia pinea to inhibit Armillaria sp., Bjerkandera adusta, Botrytis cinerea, an... more The ability of Diplodia pinea to inhibit Armillaria sp., Bjerkandera adusta, Botrytis cinerea, and Rhizoctonia sp. mycelium growth was analyzed using the double-culture method. Wild-type fungal strains were incubated in a biochemical oxygen demand incubator using potato agar dextrose medium at 24 ± 2 °C for 35 days in darkness. D. pinea significantly inhibited the growth of all fungi species tested (30.75 to 98.37% inhibition) and showed moderate antagonistic activity (antagonistic index, 14.5). Chemical analysis of D. pinea culture broth extracts revealed steroids, triterpenes, and phenolic compounds. Alkaloids were qualitatively detected in the mycelium crude extract. The presence of these compounds may be related to the antagonistic activity observed. The inhibition ability of D. pinea is due to competition with the tested fungi for substrate and space.
There have been few studies on the pharmacological properties of var. , a native Brazilian specie... more There have been few studies on the pharmacological properties of var. , a native Brazilian species popularly known as "fruto-de-pombo." The aim of this study was to investigate the scavenging capacity of emodin, physcion, and the ethanolic crude extract of var. against reactive oxygen and nitrogen species, as well as their role and plausible mechanisms in prompting cell death and changes in AKT phosphorylation after cervical (SiHa and C33A) and oral (HSC-3) squamous cell carcinoma treatments. Emodin was shown to be the best scavenger of NO and O, while all samples were equally effective in HOCl/OCl capture. Emodin, physcion, and the ethanolic extract all exhibited cytotoxic effects on SiHa, C33A, HSC-3, and HaCaT (immortalized human keratinocytes, nontumorigenic cell line), involving mixed cell death (apoptosis and necrosis) independent of the caspase activation pathway. Emodin, physcion, and the ethanolic extract increased intracellular oxidative stress and DNA damage. Em...
Antimicrobial resistance is a critical health problem, and pathogens responsible for common infec... more Antimicrobial resistance is a critical health problem, and pathogens responsible for common infections have developed resistance to antimicrobials, posing a threat to global health and placing a huge burden on health services. During the past two decades, the search for new bioactive agents in nature has become extremely important for promoting health and in the development of more efficient antimicrobials. The genus is one of the largest in the Myrtaceae family, comprising approximately 1000 species from Mexico to Argentina, with a few species distributed in Australia and Africa. species are used in folk medicine, with antidiabetic, antirheumatic, antipyretic, anti-inflammatory, antidiarrheal, antifungal, and antibacterial properties. This study systematically reviews the species to compile the phytochemical composition and antimicrobial effects. In addition, we provide information regarding the traditional uses and cytotoxic activity of species. We conducted a systematic literatur...
Acta Crystallographica Section C Crystal Structure Communications
... OBDULIO G. MIGUEL,a MOACIR G. PIZZOLA"ITI,a ROSENDO A. YUNES,a IVO VENCATO,b... more ... OBDULIO G. MIGUEL,a MOACIR G. PIZZOLA"ITI,a ROSENDO A. YUNES,a IVO VENCATO,b JO,KO B. CALIXTO,c ADAIR RS SANTOS,c ADRIANA CONTINd AND EDUARDO A. MOREIRAd. "Depto. ... C., Guagliardi, A.. Burla, M. C,, Polidori, G. & Camalli. M. (1994). J. Appl. Crxst. ...
Toxicology in vitro : an international journal published in association with BIBRA, Jan 2, 2017
The aim of this study was to investigate the cytotoxic activity of the essential oil from cladode... more The aim of this study was to investigate the cytotoxic activity of the essential oil from cladodes of Bacharis milleflora in relation to Jurkat, Raji and HL-60 cells, as well as exploring the cell mechanisms in order to elucidate how the cytotoxic process occurs. The presence of the following volatile compounds was detected by GC-MS: bicyclogermacrene (12.16%), germacrene D (11.18%), (E)-caryophyllene (9.28%), and α-humulene (8.05%). In general, IC50 values lower than 50μg/mL were obtained for all the tumor cells at 24, 48 and 72h by MTT assay. The decrease in cell DNA content was demonstrated due to the inhibition of the proliferation of Jurkat, Raji and HL-60 cells by B. milleflora essential oil. In particular, Raji cells presented the greatest inhibition of cell proliferation and they were subsequently used to investigate cell death mechanisms. B. milleflora essential oil promoted G0/G1 arrest and also induced cell fragmentation, which was represented by an increase in the sub-G0...
Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with ... more Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, Byrsonima duckeana W. R. Anderson is an Amazonian species almost not studied. Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities of Byrsonima duckeana leaves. Materials and Methods. We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays. Results. All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples. B. duckeana was able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in t...
Evidence-based complementary and alternative medicine : eCAM, 2016
The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically u... more The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of S. larvata were phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract of S. larvata revealed the presence of three flavonoids, drabanemoroside, kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-α-L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compounds p-hydroxybenzoic acid and p-coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed...
Ecotoxicology and environmental safety, Jan 30, 2017
The frequent use of synthetic pesticides to control Aedes aegypti population can lead to environm... more The frequent use of synthetic pesticides to control Aedes aegypti population can lead to environmental and/or human contamination and the emergence of resistant insects. Linalool and methyl cinnamate are presented as an alternative to the synthetic pesticides, since they can exhibit larvicidal, repellent and/or insecticidal activity and are considered safe for use. The aim of this study was to evaluate the larvicidal activity of methyl cinnamate, linalool and methyl cinnamate/linalool in combination (MC-L) (1:4 ratio, respectively) against Aedes aegypti. The in vitro preliminary toxicity through brine shrimp lethality assay and hemolytic activity, and the phytotoxic potential were also investigated to assess the safety of their use as larvicide. Methyl cinnamate showed significant larvicidal activity when compared to linalool (LC50 values of 35.4µg/mL and 275.2µg/mL, respectively) and to MC-L (LC50 138.0µg/mL). Larvae morphological changes subjected to the specified treatments were ...
As plantas são utilizadas por muitos povos como recurso à terapia de doenças, dessa forma, tem se... more As plantas são utilizadas por muitos povos como recurso à terapia de doenças, dessa forma, tem se intensificado as pesquisas por substâncias provindas de plantas e compostos naturais que possam ser utilizadas como fármacos. Os valores de umidade, cinzas totais e os resultados da investigação fitoquímica são essenciais para o controle de qualidade e para a caracterização das espécies vegetais. Temnadenia stellaris, planta relatada de forma endêmica no Brasil, é uma liana pertencente à família Apocynaceae, as plantas desta família são descritas como potenciais fontes de substâncias bioativas devido à importância dada aos metabólitos secundários já encontrados. Tendo em vista os poucos estudos envolvendo a espécie, foram realizados testes para determinar os parâmetros fitoquímicos da planta. O caule de Temnadenia stellaris apresentou umidade de 7,7%, enquanto que as folhas tiveram 11,88% de umidade segundo o ensaio realizado. O caule de Temnadenia stellaris apresentou teor de cinzas de...
Page 1. Revista Lecta, Bragança Paulista, v. 21, n. 1/2, p. 7-13, jan./dez. 2003 7 Fitoterápicos:... more Page 1. Revista Lecta, Bragança Paulista, v. 21, n. 1/2, p. 7-13, jan./dez. 2003 7 Fitoterápicos: uma abordagem farmacotécnica Ana Cristina Oltramari Toledo1 Lilian Lúcio Hirata1 Marilene da Cruz M. Buffon 2, 3 Marilis Dallarmi Miguel 4 Obdulio Gomes Miguel 4 ...
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