Single crystal of L-Glycine Formate (LGF) was grown by slow evaporation method. Dielectric consta... more Single crystal of L-Glycine Formate (LGF) was grown by slow evaporation method. Dielectric constant and dielectric loss have been obtained as a function of frequency between 50 Hz -5 MHz and temperature range between 35oC-75oC. The dependence of tan δ, e ״ and ζac on temperature and frequency of the applied field (50 Hz-5 MHz) is established D.C. conductivity has been deduced from the A.C. conductivity data and activation energy is calculated.
Copper sulphide (CuS) thin films are deposited on microscopic glass substrate by chemical techniq... more Copper sulphide (CuS) thin films are deposited on microscopic glass substrate by chemical technique called successive ionic layer adsorption and reaction (SILAR) method at room temperature. The technique involves multiple dipping of the substrate in an aqueous solution of copper nitrate, sodium sulphide and in deionised water. The aim of this study is to establish the optical properties (Absorbance, Transmittance and Band gap) of Copper sulphide thin films by UV-VIS spectrometer and the influence of molar concentration was studied to determine the optimum condition for deposition process. The band gap energy decreases from 2.26 V and 1.86 eV when molar concentration increases from 0.05M to0.25M. The high absorbance of the films made them good materials for conversion of solar energy.
India is one of the richest countries in the world with large and wide varieties of medicinal pla... more India is one of the richest countries in the world with large and wide varieties of medicinal plants and considered to be the motherland for many traditional plants of medicinal values. Nature has been a source of medicinal agents for thousands of years and a remarkable number of modem drugs have been isolated from them, many based on their use in traditional medicine. They have the capacity to produce a large number of bioactive compounds with complex structural diversity known as secondary metabolites. The present study was carried out to investigate the potential bioactive compounds present in ethanol extract of Ipomoea sepiaria leaves using phytochemical analysis and Gas Chromatography Mass Spectrometry analysis. The GC-MS analysis revealed the presence of 20 compounds and many of these compounds have antimicrobial, antidiabetic, anti-inflammatory, antioxidant and anticancer activities. Medicinal plants with these components are very essential for treating various diseases and d...
Antibody targeted cytoplasmic delivery of drugs is difficult to achieve as antigen-antibody inter... more Antibody targeted cytoplasmic delivery of drugs is difficult to achieve as antigen-antibody interaction results in the payload being directed to the endosomal compartment. However, Sendai viral envelopes can bring about cytoplasmic delivery due to F-protein mediated membrane fusion. In this study we have generated and fused a recombinant scFv directed to the onco-fetal antigen, the Placental isozyme of Alkaline Phosphatase (PAP) with the trans-membrane and part of the cytoplasmic domain of the Sendai F protein (F(TMC)). Reconstituted virosomes, having both the fusion protein as well as the native F-protein were able to specifically bind and deliver drugs to PAP expressing cells. About 75% of the delivery was cytoplasmic in nature. Hence, this immuno-virosome, which is devoid of the comparatively more toxic HN protein, has the novel ability to combine specific antibody mediated targeting with cytoplasmic delivery. The scFv ensured specific binding to PAP expressing cells, without cross reacting with the other isozymes of alkaline phosphatase. The advantages of cytoplasmic delivery would include reduced degradation and lowered immunogenicity of the payload and carrier. The ubiquitous expression of PAP on a variety of cancers like seminoma, choriocarcinoma, cervical and breast cancers also suggests its potential usefulness in a number of malignancies.
To verify if Leptospira hardjo can colonize the male and female genital organs of sheep, 9 animal... more To verify if Leptospira hardjo can colonize the male and female genital organs of sheep, 9 animals (6 non pregnant ewes and 3 mature rams) were infected with a strain of L. hardjobovis recently recovered from the kidneys of a seropositive ewe. Postinfection controls (bacteriologic, serologic, immunohistochemistry and electron microscopy) failed to disclose the presence of leptospires in the uterus and oviducts, testicles, epididymis, prostate and bulbourethral glands of animals used for the experiment and slaughtered from 37 to 242 postinfection days. All animals showed a renal localization of L. hardjobovis lasting for the entire period of the study (over 8 months). These results emphasize the important role of sheep as maintenance hosts of the serovar.
Single crystal of L-Glycine Formate (LGF) was grown by slow evaporation method. Dielectric consta... more Single crystal of L-Glycine Formate (LGF) was grown by slow evaporation method. Dielectric constant and dielectric loss have been obtained as a function of frequency between 50 Hz -5 MHz and temperature range between 35oC-75oC. The dependence of tan δ, e ״ and ζac on temperature and frequency of the applied field (50 Hz-5 MHz) is established D.C. conductivity has been deduced from the A.C. conductivity data and activation energy is calculated.
Copper sulphide (CuS) thin films are deposited on microscopic glass substrate by chemical techniq... more Copper sulphide (CuS) thin films are deposited on microscopic glass substrate by chemical technique called successive ionic layer adsorption and reaction (SILAR) method at room temperature. The technique involves multiple dipping of the substrate in an aqueous solution of copper nitrate, sodium sulphide and in deionised water. The aim of this study is to establish the optical properties (Absorbance, Transmittance and Band gap) of Copper sulphide thin films by UV-VIS spectrometer and the influence of molar concentration was studied to determine the optimum condition for deposition process. The band gap energy decreases from 2.26 V and 1.86 eV when molar concentration increases from 0.05M to0.25M. The high absorbance of the films made them good materials for conversion of solar energy.
India is one of the richest countries in the world with large and wide varieties of medicinal pla... more India is one of the richest countries in the world with large and wide varieties of medicinal plants and considered to be the motherland for many traditional plants of medicinal values. Nature has been a source of medicinal agents for thousands of years and a remarkable number of modem drugs have been isolated from them, many based on their use in traditional medicine. They have the capacity to produce a large number of bioactive compounds with complex structural diversity known as secondary metabolites. The present study was carried out to investigate the potential bioactive compounds present in ethanol extract of Ipomoea sepiaria leaves using phytochemical analysis and Gas Chromatography Mass Spectrometry analysis. The GC-MS analysis revealed the presence of 20 compounds and many of these compounds have antimicrobial, antidiabetic, anti-inflammatory, antioxidant and anticancer activities. Medicinal plants with these components are very essential for treating various diseases and d...
Antibody targeted cytoplasmic delivery of drugs is difficult to achieve as antigen-antibody inter... more Antibody targeted cytoplasmic delivery of drugs is difficult to achieve as antigen-antibody interaction results in the payload being directed to the endosomal compartment. However, Sendai viral envelopes can bring about cytoplasmic delivery due to F-protein mediated membrane fusion. In this study we have generated and fused a recombinant scFv directed to the onco-fetal antigen, the Placental isozyme of Alkaline Phosphatase (PAP) with the trans-membrane and part of the cytoplasmic domain of the Sendai F protein (F(TMC)). Reconstituted virosomes, having both the fusion protein as well as the native F-protein were able to specifically bind and deliver drugs to PAP expressing cells. About 75% of the delivery was cytoplasmic in nature. Hence, this immuno-virosome, which is devoid of the comparatively more toxic HN protein, has the novel ability to combine specific antibody mediated targeting with cytoplasmic delivery. The scFv ensured specific binding to PAP expressing cells, without cross reacting with the other isozymes of alkaline phosphatase. The advantages of cytoplasmic delivery would include reduced degradation and lowered immunogenicity of the payload and carrier. The ubiquitous expression of PAP on a variety of cancers like seminoma, choriocarcinoma, cervical and breast cancers also suggests its potential usefulness in a number of malignancies.
To verify if Leptospira hardjo can colonize the male and female genital organs of sheep, 9 animal... more To verify if Leptospira hardjo can colonize the male and female genital organs of sheep, 9 animals (6 non pregnant ewes and 3 mature rams) were infected with a strain of L. hardjobovis recently recovered from the kidneys of a seropositive ewe. Postinfection controls (bacteriologic, serologic, immunohistochemistry and electron microscopy) failed to disclose the presence of leptospires in the uterus and oviducts, testicles, epididymis, prostate and bulbourethral glands of animals used for the experiment and slaughtered from 37 to 242 postinfection days. All animals showed a renal localization of L. hardjobovis lasting for the entire period of the study (over 8 months). These results emphasize the important role of sheep as maintenance hosts of the serovar.
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