Measurement of fluorescence increase was used for the comparative quantification of the effect th... more Measurement of fluorescence increase was used for the comparative quantification of the effect that several cyclodextrins (alpha-, beta-, heptakis-2,6-beta-omicron-dimethyl- and gamma-) produce on the fluorescent response of aflatoxins B1 and G1. This constitutes a new chromatographic method with stability of the mobile phase, and shows general improvements in the chromatographic conditions with respect to other methods (especially those using an iodine reservoir as a postcolumn reactor). A C18-type column was used, with methanol-water (60:40, v/v) as the mobile phase. The excitation phase of the natural fluorescence of aflatoxins, a 10(-2) M solution of each cyclodextrin, was introduced postcolumn. The determination of the elution order aflatoxin G2 > G1 > B2 > B1 was performed for each phase in less than 15 min. As expected using an aqueous-alcoholic medium, an increase in the fluorescence response of aflatoxins with an unsaturated furanic ring was found to occur with all the cyclodextrins studied, except gamma-cyclodextrin. The observed increase was larger for heptakis-2,6-beta-omicron-dimethyl- than for beta-cyclodextrin (to our knowledge, the only cyclodextrin previously described in the literature to serve for the determination of aflatoxins). The difference is of the order of 70.1-fold in the case of aflatoxin G1 and 45.2-fold in the case of aflatoxin B1. The detection limit in the mobile phase used was determined (for aflatoxin B1) for beta-cyclodextrin and 2,6-beta-omicron-dimethylcyclodextrin (signal-to-noise ratio 1:3) to be 4 and 9 mg 1(-1), respectively.
ABSTRACT The simultaneous determination in aqueous media of the gadolinium ion (Gd3+) and the mac... more ABSTRACT The simultaneous determination in aqueous media of the gadolinium ion (Gd3+) and the macrocyclic chelate Gd–DOTA (DOTA = 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid) used in magnetic resonance imaging (MRI) is described. The proposed approach combines a reversed-phase high-performance liquid chromatographic (RP-HPLC) separation and time-resolved luminescence detection (TRL). The spectroscopic properties of Gd–DOTA and various other Gd chelates (Gd–CDTA, Gd–TETA, Gd–EDTA, Gd–DTPA and Gd–TTHA) (CDTA = cyclohexanediaminetetraacetic acid; TETA = 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetra- acetic acid; EDTA = ethylenediaminetetraacetic acid; DTPA = diethylenetriaminepentaacetic acid; TTHA = triethylenetetraaminehexaacetic acid) were established in order to optimize TRL detection (λexc = 274 nm, λem = 313 nm, delay time 0.1 ms, gate time 10 ms). The Gd3+ ion possibly present in the matrix was detected as a Gd–CDTA chelate after dilution of the sample by a pH 7.6-buffered 1 × 10–2M aqueous CDTA solution. Then, Gd–DOTA and Gd3+ ion (as Gd–CDTA chelate) were chromatographed on an RP C8 HPLC column. The eluent consisted of pH 7.6 TRIS–HCl buffer + acetonitrile (98 + 2 v/v). The analytical figures of merit obtained for Gd–DOTA in aqueous solution were as follows: linearity was assumed from 10 to 5000 µmol l–1; the limit of detection (LOD, S/N = 3) was 21 µmol l–1, which corresponds to 235 ng of Gd–DOTA injected. The repeatability (RSD = 10%, n = 15) and the intra-assay reproducibility (RSD = 12%, n = 6) were studied for a 50 µmol l–1 solution. With this method, 130 ng of Gd3+ ion (as Gd–CDTA) injected can be detected, which corresponds to an LOD of 42 µmol l–1. The LOQ for Gd3+ ion was 140 µmol l–1. Consequently, Gd3+ ion can be distinguished from chelated Gd–DOTA in aqueous solution (at the 0.2% level).
Abstract The inclusion into β-cyclodextrin (β-Cyd) by the two methoxypsoralen isomers (5-methoxyp... more Abstract The inclusion into β-cyclodextrin (β-Cyd) by the two methoxypsoralen isomers (5-methoxypsoralen (5-MOP) and 8-methoxypsoralen (8-MOP)) has been extensively studied by reverse phase liquid chromatography (RP-HPLC). Some important quantitative data concerning the complexation in a dynamic flow system have been drawn. The stoichiometry of the complex formation (1:1 in all instances), and the affinity constants with the β-Cyd cavity (Kf) of the two guest compounds, have been established. By taking into account the competitive effect of the organic modifier of the eluent (methanol), the Kfs of both compounds have been extrapolated to pure water (233 M−1 and 106 M−1 for 5-MOP and 8-MOP, respectively). On the other hand, the established stoichiometry and affinity constants have been interpreted in terms of enthalpy and entropy changes. Finally, the importance of the position of the methoxy substituent has been underlined and seems to rule the quality of the fit of each isomer within the inner cavity of ...
Abstract This paper describes a simple and rapid procedure for the determination of the important... more Abstract This paper describes a simple and rapid procedure for the determination of the important tripeptide glycyl-leucyl-phenylalanine (GLF) and its homologues by reversed phase high-performance liquid chromatography. The eluent for convenient separation on a phenyl-bonded silica stationary phase was 15/85 (v/v) acetonitrile/0.1 M sodium citrate buffer pH 3.0, UV detection was set at 258 nm. The method is specific, linear, accurate (recovery mean=99.6±0.8%), and reliable (precision=1.2%). Limit of detection is established for 0.05 mM and limit of quantification for 0.10 mM (precision=1.5%).
In order to improve its quality-assurance programme based on ISO 9001, the Central Sterile Supply... more In order to improve its quality-assurance programme based on ISO 9001, the Central Sterile Supply Department of a public university hospital has performed a prospective risk analysis using the Preliminary Risk Analysis method (PRA). The objectives were the achievement of a global risk mapping related to the whole process of sterilising medical devices, and second, the implementation of corrective measures to reduce identified risks. A multidisciplinary team, formed in January 2008, validated results at each step of the study. During the analysis, 416 hazardous situations were identified, among which 81 were quoted first in priority and led to the description of 141 risk scenarios. The PRA team assessed 42 scenarios with risk ranking assessed as 'acceptable under control' or 'unacceptable.' They adopted 23 follow-up actions measures and 13 safety parameters. The PRA constitutes an appropriate tool for assessing quality-improvement policy and safety in healthcare facilities that can be easily integrated into standard quality-management systems.
This study concerned the use of lanthanide chelates to detect glycyl-leucyl-phenylalanine (GLF) a... more This study concerned the use of lanthanide chelates to detect glycyl-leucyl-phenylalanine (GLF) and its homologues. Spectroscopic analysis of peptides without or with terbium complexation revealed the formation of (LF)3(Tb)2, (GF)3(Tb)2, (GLF)3(Tb)2 and (FL)4Tb, (FG)4Tb complexes with high stability constants in methanolic solutions (pKd>13). Lanthanide chelate emission displayed a large Stokes shift (>270 nm), which allowed Tb chelates of GLF and
International Journal of Technology Assessment in Health Care, 2021
Objective We aimed to perform a systematic review of economic evaluations of drug-coated balloons... more Objective We aimed to perform a systematic review of economic evaluations of drug-coated balloons (DCBs) and drug-eluting stents (DESs) in peripheral artery disease (PAD) and to assess the level of evidence of relevant studies. The purpose was not to present economic findings. Methods A systematic review was performed using four electronic databases to identify health economic evaluation studies reporting on the use of DCBs and DESs in PAD. The methodological and reporting quality of the studies was assessed using three different tools, the Drummond, Cooper, and CHEERS (Consolidated Health Economic Evaluation Reporting Standards) checklists. Results Six articles were included in this review of the 1,728 publications identified. Four studies were cost-effectiveness analyses and two cost–utility analyses. According to the Cooper hierarchy scale, the studies used good-quality data sources. The level of evidence used for clinical effect sizes, safety data, baseline clinical data, and co...
Molecular Engineering for Advanced Materials, 1995
The Cyclodextrins are a class of “off-the-shelf” [1] molecular hosts prepared by the enzymatic de... more The Cyclodextrins are a class of “off-the-shelf” [1] molecular hosts prepared by the enzymatic degradation of amylose by the enzyme CGTase. Their molecular dimensions, symetry and general shape as truncated cones are schematised below in Figure 1.
While home-based chemotherapy improves comfort and quality of life of patients, quality and safet... more While home-based chemotherapy improves comfort and quality of life of patients, quality and safety conditions must be equivalent to hospital settings. In addition, organization is much more complex. At the hospital at home "Assistance publique-Hôpitaux de Paris", prescribers are potentially spread across 21 health facilities. The administration of chemotherapy is performed by about 300 nurses at the patient's home in Paris and its suburbs. Centralized preparations of chemotherapy began in September 2009 by the pharmacy department of Georges-Pompidou European hospital, with a progressive increase of the activity. This article describes the quality insurance system established with this new organization to meet the specific challenges of home therapy: choice of eligible anticancer drugs, computerized information systems and networking with other heath facilities, secure transport conditions, traceability from the prescription to the administration, security of administra...
Measurement of fluorescence increase was used for the comparative quantification of the effect th... more Measurement of fluorescence increase was used for the comparative quantification of the effect that several cyclodextrins (alpha-, beta-, heptakis-2,6-beta-omicron-dimethyl- and gamma-) produce on the fluorescent response of aflatoxins B1 and G1. This constitutes a new chromatographic method with stability of the mobile phase, and shows general improvements in the chromatographic conditions with respect to other methods (especially those using an iodine reservoir as a postcolumn reactor). A C18-type column was used, with methanol-water (60:40, v/v) as the mobile phase. The excitation phase of the natural fluorescence of aflatoxins, a 10(-2) M solution of each cyclodextrin, was introduced postcolumn. The determination of the elution order aflatoxin G2 > G1 > B2 > B1 was performed for each phase in less than 15 min. As expected using an aqueous-alcoholic medium, an increase in the fluorescence response of aflatoxins with an unsaturated furanic ring was found to occur with all the cyclodextrins studied, except gamma-cyclodextrin. The observed increase was larger for heptakis-2,6-beta-omicron-dimethyl- than for beta-cyclodextrin (to our knowledge, the only cyclodextrin previously described in the literature to serve for the determination of aflatoxins). The difference is of the order of 70.1-fold in the case of aflatoxin G1 and 45.2-fold in the case of aflatoxin B1. The detection limit in the mobile phase used was determined (for aflatoxin B1) for beta-cyclodextrin and 2,6-beta-omicron-dimethylcyclodextrin (signal-to-noise ratio 1:3) to be 4 and 9 mg 1(-1), respectively.
ABSTRACT The simultaneous determination in aqueous media of the gadolinium ion (Gd3+) and the mac... more ABSTRACT The simultaneous determination in aqueous media of the gadolinium ion (Gd3+) and the macrocyclic chelate Gd–DOTA (DOTA = 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid) used in magnetic resonance imaging (MRI) is described. The proposed approach combines a reversed-phase high-performance liquid chromatographic (RP-HPLC) separation and time-resolved luminescence detection (TRL). The spectroscopic properties of Gd–DOTA and various other Gd chelates (Gd–CDTA, Gd–TETA, Gd–EDTA, Gd–DTPA and Gd–TTHA) (CDTA = cyclohexanediaminetetraacetic acid; TETA = 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetra- acetic acid; EDTA = ethylenediaminetetraacetic acid; DTPA = diethylenetriaminepentaacetic acid; TTHA = triethylenetetraaminehexaacetic acid) were established in order to optimize TRL detection (λexc = 274 nm, λem = 313 nm, delay time 0.1 ms, gate time 10 ms). The Gd3+ ion possibly present in the matrix was detected as a Gd–CDTA chelate after dilution of the sample by a pH 7.6-buffered 1 × 10–2M aqueous CDTA solution. Then, Gd–DOTA and Gd3+ ion (as Gd–CDTA chelate) were chromatographed on an RP C8 HPLC column. The eluent consisted of pH 7.6 TRIS–HCl buffer + acetonitrile (98 + 2 v/v). The analytical figures of merit obtained for Gd–DOTA in aqueous solution were as follows: linearity was assumed from 10 to 5000 µmol l–1; the limit of detection (LOD, S/N = 3) was 21 µmol l–1, which corresponds to 235 ng of Gd–DOTA injected. The repeatability (RSD = 10%, n = 15) and the intra-assay reproducibility (RSD = 12%, n = 6) were studied for a 50 µmol l–1 solution. With this method, 130 ng of Gd3+ ion (as Gd–CDTA) injected can be detected, which corresponds to an LOD of 42 µmol l–1. The LOQ for Gd3+ ion was 140 µmol l–1. Consequently, Gd3+ ion can be distinguished from chelated Gd–DOTA in aqueous solution (at the 0.2% level).
Abstract The inclusion into β-cyclodextrin (β-Cyd) by the two methoxypsoralen isomers (5-methoxyp... more Abstract The inclusion into β-cyclodextrin (β-Cyd) by the two methoxypsoralen isomers (5-methoxypsoralen (5-MOP) and 8-methoxypsoralen (8-MOP)) has been extensively studied by reverse phase liquid chromatography (RP-HPLC). Some important quantitative data concerning the complexation in a dynamic flow system have been drawn. The stoichiometry of the complex formation (1:1 in all instances), and the affinity constants with the β-Cyd cavity (Kf) of the two guest compounds, have been established. By taking into account the competitive effect of the organic modifier of the eluent (methanol), the Kfs of both compounds have been extrapolated to pure water (233 M−1 and 106 M−1 for 5-MOP and 8-MOP, respectively). On the other hand, the established stoichiometry and affinity constants have been interpreted in terms of enthalpy and entropy changes. Finally, the importance of the position of the methoxy substituent has been underlined and seems to rule the quality of the fit of each isomer within the inner cavity of ...
Abstract This paper describes a simple and rapid procedure for the determination of the important... more Abstract This paper describes a simple and rapid procedure for the determination of the important tripeptide glycyl-leucyl-phenylalanine (GLF) and its homologues by reversed phase high-performance liquid chromatography. The eluent for convenient separation on a phenyl-bonded silica stationary phase was 15/85 (v/v) acetonitrile/0.1 M sodium citrate buffer pH 3.0, UV detection was set at 258 nm. The method is specific, linear, accurate (recovery mean=99.6±0.8%), and reliable (precision=1.2%). Limit of detection is established for 0.05 mM and limit of quantification for 0.10 mM (precision=1.5%).
In order to improve its quality-assurance programme based on ISO 9001, the Central Sterile Supply... more In order to improve its quality-assurance programme based on ISO 9001, the Central Sterile Supply Department of a public university hospital has performed a prospective risk analysis using the Preliminary Risk Analysis method (PRA). The objectives were the achievement of a global risk mapping related to the whole process of sterilising medical devices, and second, the implementation of corrective measures to reduce identified risks. A multidisciplinary team, formed in January 2008, validated results at each step of the study. During the analysis, 416 hazardous situations were identified, among which 81 were quoted first in priority and led to the description of 141 risk scenarios. The PRA team assessed 42 scenarios with risk ranking assessed as 'acceptable under control' or 'unacceptable.' They adopted 23 follow-up actions measures and 13 safety parameters. The PRA constitutes an appropriate tool for assessing quality-improvement policy and safety in healthcare facilities that can be easily integrated into standard quality-management systems.
This study concerned the use of lanthanide chelates to detect glycyl-leucyl-phenylalanine (GLF) a... more This study concerned the use of lanthanide chelates to detect glycyl-leucyl-phenylalanine (GLF) and its homologues. Spectroscopic analysis of peptides without or with terbium complexation revealed the formation of (LF)3(Tb)2, (GF)3(Tb)2, (GLF)3(Tb)2 and (FL)4Tb, (FG)4Tb complexes with high stability constants in methanolic solutions (pKd>13). Lanthanide chelate emission displayed a large Stokes shift (>270 nm), which allowed Tb chelates of GLF and
International Journal of Technology Assessment in Health Care, 2021
Objective We aimed to perform a systematic review of economic evaluations of drug-coated balloons... more Objective We aimed to perform a systematic review of economic evaluations of drug-coated balloons (DCBs) and drug-eluting stents (DESs) in peripheral artery disease (PAD) and to assess the level of evidence of relevant studies. The purpose was not to present economic findings. Methods A systematic review was performed using four electronic databases to identify health economic evaluation studies reporting on the use of DCBs and DESs in PAD. The methodological and reporting quality of the studies was assessed using three different tools, the Drummond, Cooper, and CHEERS (Consolidated Health Economic Evaluation Reporting Standards) checklists. Results Six articles were included in this review of the 1,728 publications identified. Four studies were cost-effectiveness analyses and two cost–utility analyses. According to the Cooper hierarchy scale, the studies used good-quality data sources. The level of evidence used for clinical effect sizes, safety data, baseline clinical data, and co...
Molecular Engineering for Advanced Materials, 1995
The Cyclodextrins are a class of “off-the-shelf” [1] molecular hosts prepared by the enzymatic de... more The Cyclodextrins are a class of “off-the-shelf” [1] molecular hosts prepared by the enzymatic degradation of amylose by the enzyme CGTase. Their molecular dimensions, symetry and general shape as truncated cones are schematised below in Figure 1.
While home-based chemotherapy improves comfort and quality of life of patients, quality and safet... more While home-based chemotherapy improves comfort and quality of life of patients, quality and safety conditions must be equivalent to hospital settings. In addition, organization is much more complex. At the hospital at home "Assistance publique-Hôpitaux de Paris", prescribers are potentially spread across 21 health facilities. The administration of chemotherapy is performed by about 300 nurses at the patient's home in Paris and its suburbs. Centralized preparations of chemotherapy began in September 2009 by the pharmacy department of Georges-Pompidou European hospital, with a progressive increase of the activity. This article describes the quality insurance system established with this new organization to meet the specific challenges of home therapy: choice of eligible anticancer drugs, computerized information systems and networking with other heath facilities, secure transport conditions, traceability from the prescription to the administration, security of administra...
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