It has been proposed that on insertion, an intrauterine contraceptive device (IUCD) becomes coate... more It has been proposed that on insertion, an intrauterine contraceptive device (IUCD) becomes coated in a mucus biofilm which offers protection to those bacteria which have been introduced into the uterus from the vagina (Malhi et al, 1989). The incidence of uterine infection is highest in the first few months after fitting the IUCD and in attempt to combat this,antibiotic cover is recommended at the time of insertion. Despite this, pelvic infections have been shown to occur in IUCD users twice as frequently as in women not using contraception (Population Report,1988). The value of antibiotic prophylaxis, however, could be questioned since penetration of systemically administered antibiotics into mucus is poor (Kearney & Marriott, 1987). We have therefore investigated ways in which antimicrobial agents can be delivered directly to the required site.
Pharmacy and Pharmacology Communications, Oct 1, 1995
Evening primrose oil (EPO), and its putative active component γ-linolenic acid (GLA), are used fo... more Evening primrose oil (EPO), and its putative active component γ-linolenic acid (GLA), are used for the treatment of cyclical and non-cyclical mastalgia; however the mechanism of action is unknown. This study addressed the effects of EPO and GLA on the electrical properties of the membranes of NG108-15 neuroblastoma x glioma hybrid cells and MCF-7 breast epithelial cells. GLA, but not EPO, inhibited potassium M currents in NG108-15 cells. MCF-7 cells did not exhibit M currents and no obvious electrophysiological effects of either GLA or EPO were observed. The disparity between the two oils suggests that these results are unrelated to the actions of the two oils in the treatment of mastalgia.
Journal of The Saudi Pharmaceutical Society, Dec 1, 2021
Brain derived neutrophic factor (BDNF) is a protein and a member of the neurotrophin family of gr... more Brain derived neutrophic factor (BDNF) is a protein and a member of the neurotrophin family of growth factors, supports the survival of existing neurons and encourages the growth and differentiation of new neurons and synapses. The BDNF gene Val66Met polymorphism (rs6265/G196A) is responsible for BDNF synthesis that impact BDNF function that includes memory and cognition. This study investigated whether the BDNF gene Val66Met polymorphism (rs6265/G196A) is associated with cognitive function changes in both Alzheimer disease (AD) patients and elderly participants. In addition the impact of SSRI use on cognition improvement will be assessed. Healthy young, middle ages (25–59 years old) and elderly (more than 60 years old) participants (140) as well as 40 AD patients of whom are both of Saudi Arabian origin were recruited. The genotyping for the association study was performed by real-time PCR using Taqman chemistry in the ABI Prism 7900HT Sequence Detection System. Both Mini-Mental Status Examination (MMSE) and Clinical Dementia Rating (CDR) were used to assess cognitive function of healthy and AD participants, respectively. The findings showed that the BDNF Val66Met genotype distributions and allele frequencies have significant association with cognitive performance in both elderly control group and AD patients. The main findings showed that carriers of GG homozygotes (Val/Val) have superior cognitive performance among AD patients and elderly control subjects. In addition the use of SSRIs in 13 AD patients and 17 elderly participants positively improved cognitive function in elderly (p > 0.001) but not in AD patients (p = 0.1).
Implantation of intrauterine devices (IUDs) is associated with an increased incidence of uterine ... more Implantation of intrauterine devices (IUDs) is associated with an increased incidence of uterine infection, probably as a result of vaginal bacteria entering the uterus at the time of insertion. To reduce the incidence of IUD-related infections, the incorporation of antimicrobial agents into the tail of the device was studied. Chlorhexidine was shown to be released from within nylon hollow fibres at a rate of approximately 114 μg·day–1 for 10 days. This rate of release was sufficient to exhibit a biocidal effect on bacteria embedded within a mucus gel in vitro. When these devices were implanted transcervically into the guinea-pig uterus they significantly reduced the extent of uterine bacterial contamination within 24 h. These findings indicate that chlorhexidine-releasing devices are potentially useful in the reduction of device-related infections.
It has been proposed that on insertion, an intrauterine contraceptive device (IUCD) becomes coate... more It has been proposed that on insertion, an intrauterine contraceptive device (IUCD) becomes coated in a mucus biofilm which offers protection to those bacteria which have been introduced into the uterus from the vagina (Malhi et al, 1989). The incidence of uterine infection is highest in the first few months after fitting the IUCD and in attempt to combat this,antibiotic cover is recommended at the time of insertion. Despite this, pelvic infections have been shown to occur in IUCD users twice as frequently as in women not using contraception (Population Report,1988). The value of antibiotic prophylaxis, however, could be questioned since penetration of systemically administered antibiotics into mucus is poor (Kearney & Marriott, 1987). We have therefore investigated ways in which antimicrobial agents can be delivered directly to the required site.
Pharmacy and Pharmacology Communications, Oct 1, 1995
Evening primrose oil (EPO), and its putative active component γ-linolenic acid (GLA), are used fo... more Evening primrose oil (EPO), and its putative active component γ-linolenic acid (GLA), are used for the treatment of cyclical and non-cyclical mastalgia; however the mechanism of action is unknown. This study addressed the effects of EPO and GLA on the electrical properties of the membranes of NG108-15 neuroblastoma x glioma hybrid cells and MCF-7 breast epithelial cells. GLA, but not EPO, inhibited potassium M currents in NG108-15 cells. MCF-7 cells did not exhibit M currents and no obvious electrophysiological effects of either GLA or EPO were observed. The disparity between the two oils suggests that these results are unrelated to the actions of the two oils in the treatment of mastalgia.
Journal of The Saudi Pharmaceutical Society, Dec 1, 2021
Brain derived neutrophic factor (BDNF) is a protein and a member of the neurotrophin family of gr... more Brain derived neutrophic factor (BDNF) is a protein and a member of the neurotrophin family of growth factors, supports the survival of existing neurons and encourages the growth and differentiation of new neurons and synapses. The BDNF gene Val66Met polymorphism (rs6265/G196A) is responsible for BDNF synthesis that impact BDNF function that includes memory and cognition. This study investigated whether the BDNF gene Val66Met polymorphism (rs6265/G196A) is associated with cognitive function changes in both Alzheimer disease (AD) patients and elderly participants. In addition the impact of SSRI use on cognition improvement will be assessed. Healthy young, middle ages (25–59 years old) and elderly (more than 60 years old) participants (140) as well as 40 AD patients of whom are both of Saudi Arabian origin were recruited. The genotyping for the association study was performed by real-time PCR using Taqman chemistry in the ABI Prism 7900HT Sequence Detection System. Both Mini-Mental Status Examination (MMSE) and Clinical Dementia Rating (CDR) were used to assess cognitive function of healthy and AD participants, respectively. The findings showed that the BDNF Val66Met genotype distributions and allele frequencies have significant association with cognitive performance in both elderly control group and AD patients. The main findings showed that carriers of GG homozygotes (Val/Val) have superior cognitive performance among AD patients and elderly control subjects. In addition the use of SSRIs in 13 AD patients and 17 elderly participants positively improved cognitive function in elderly (p > 0.001) but not in AD patients (p = 0.1).
Implantation of intrauterine devices (IUDs) is associated with an increased incidence of uterine ... more Implantation of intrauterine devices (IUDs) is associated with an increased incidence of uterine infection, probably as a result of vaginal bacteria entering the uterus at the time of insertion. To reduce the incidence of IUD-related infections, the incorporation of antimicrobial agents into the tail of the device was studied. Chlorhexidine was shown to be released from within nylon hollow fibres at a rate of approximately 114 μg·day–1 for 10 days. This rate of release was sufficient to exhibit a biocidal effect on bacteria embedded within a mucus gel in vitro. When these devices were implanted transcervically into the guinea-pig uterus they significantly reduced the extent of uterine bacterial contamination within 24 h. These findings indicate that chlorhexidine-releasing devices are potentially useful in the reduction of device-related infections.
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