Brazilian Journal of Veterinary Research and Animal Science, 2011
Campylobacter fetus é o agente etiológico da campilobacteriose genital bovina, uma doença sexualm... more Campylobacter fetus é o agente etiológico da campilobacteriose genital bovina, uma doença sexualmente transmissível que está associada com perdas reprodutivas em bovinos. Campylobacter coloniza a vagina e o útero e então infecta as células epiteliais do endométrio. O objetivo deste trabalho foi desenvolver um modelo ex vivo para quantificar a adesão de Campylobacter às células-alvo naturais específicas; este é um passo fundamental para o estabelecimento da infecção e estudos acerca da adesão e citotoxicidade sobre as células do hospedeiro natural não estão disponíveis. Os ensaios foram realizados a través da semeadura de Campylobacter fetus venerealis em culturas celulares epiteliais vaginais e uterinas.Células HeLa foram utilizadas como controle.A aderência bacteriana foi confirmada por microscopia óptica e a determinação da porcentagem de bactérias aderidas foi realizada em lâminas tingidas imunoquimicamente. Os resultados são apresentados como porcentagem de células com Campyloba...
La campylobacteriosis genital bovina es una enfermedad distribuida mundialmente que causa inferti... more La campylobacteriosis genital bovina es una enfermedad distribuida mundialmente que causa infertilidad y aborto. Los mecanismos relacionados con la patogenia de Campylobacter fetus sobre el embrión bovino son aún poco conocidos. Para estudiar el efecto de Campylobacter fetus venerealis en el desarrollo embrionario se utilizaron mórulas y blastocistos murinos. Las mórulas se cultivaron en medio Ham F10 como grupo control (GA; n: 97), más la adición de Campylobacter fetus venerealis (GB; n: 129), adicionado con filtrado libre de células (GC; n: 119) y con caldo Brucella (GD; n: 94). Los blastocistos se cultivaron en medio MEM, grupo control (G1: n: 24), y en MEM más suspensión de Campylobacter fetus venerealis, grupo desafiado (G2; n: 35). A partir de mórulas se evaluaron los porcentajes de diferenciación y hatching. En blastocistos se evaluaron los porcentajes de: hatching, hatched, adhesión y expansión. Los resultados se analizaron mediante Chi². La diferenciación resultó menor a la...
En Argentina, el control de la brucelosis bovina se apoya en la vacunación obligatoria de las ter... more En Argentina, el control de la brucelosis bovina se apoya en la vacunación obligatoria de las terneras con la cepa 19 de Brucella abortus y en la eliminación de los animales seropositivos con destino a faena. Las pruebas serológicas que se emplean más frecuentemente son el BPA (Buffered Plate Antigen) (prueba tamiz) y el FPA (Fluorescence Polarization Assay) (prueba confirmatoria). Ambas detectan anticuerpos anti-cadena O del polisacárido inducidos por la vacunación o por la infección con brucelas de campo. En ocasiones se han encontrado, en un contexto de ausencia de enfermedad clínica, reacciones serológicas atípicas en bovinos adultos. Las mismas han sido atribuidas a la presencia de anticuerpos de reacción cruzada inducidos por vacunas que contienen bacterias Gram negativas. Los objetivos del presente trabajo desarrollado en el modelo murino fueron: estudiar la interferencia en el diagnóstico de la brucelosis bovina generada por la administración de vacunas comerciales que conti...
The interest to develop research on the host-parasite relationship in bovine tritrichomonosis has... more The interest to develop research on the host-parasite relationship in bovine tritrichomonosis has accomplished the use of experimental models alternative to cattle. The BALB/c mouse became the most appropriate species susceptible to vaginal Tritrichomonas foetus infection requiring previous estrogenization. For the need of an experimental model without persistent estrogenization and with normal estrous cycles, the establishment and persistence of vaginal infection on BALB/c mouse with different concentrations of T. foetus in two experimental groups was evaluated. Group A was treated with 5mg of b-estradiol 3-benzoate to synchronize the estrous, 48 hours before the T. foetus vaginal inoculation, and Group B was inoculated in natural estrus. At 5-7 days after treatment, estrogenic effect decreased allowing all animals to cycle regularly during the experiment. From the first week post-infection, samples of vaginal mucus were taken from all animals during 34 weeks, in order to evaluate ...
An experimental murine model of bovine genital tritrichomonosis is described. Female mice were in... more An experimental murine model of bovine genital tritrichomonosis is described. Female mice were inoculated per vaginam with Tritrichomonas foetus and a sample of the study population was killed every 3 days up to 60 days post-infection. Microscopical changes in the reproductive organs were assessed and immunohistochemistry was used to detect T. foetus within these tissues. Lectin histochemistry was used to determine changes in the expression of carbohydrates within the reproductive mucosa. A range of microscopical changes were detected in the uterine endometrium by 10 days post-inoculation and these were associated with the presence of the protozoan. The endometrial changes included endometritis and ulceration, mucosal atrophy and glandular metaplasia, and were similar to those reported in naturally infected cows. Changes in lectin binding were recognized first in the vagina where there was increased binding of Ulex europaeus agglutinin-1 (UEA-1) which was maximal on day 16 post-inoculation. Within the uterus, there was increased binding of soy bean agglutinin (SBA) which was maximal on day 19 post-inoculation, and of peanut agglutinin (PNA) which was maximal on day 16 post-inoculation. These changes in carbohydrate expression parallel the infection kinetics, since they appeared first in the vagina and later in the uterus. The changes may reflect either a host reaction against the infection or the production of enzymes by T. foetus, which act to enhance adhesion and colonization of the genital organs by the organism. The kinetics and pathogenesis of this murine infection are similar to those of the natural bovine disease, suggesting that this model system may be valuable for further studies of this disease.
Universidad Nacional del Centro de la Provincia de Buenos Aires Sistema de Bibliotecas UNICEN Bús... more Universidad Nacional del Centro de la Provincia de Buenos Aires Sistema de Bibliotecas UNICEN Búsqueda general: Formato: Default. ... Autor : Soto, Pedro. Título : La respuesta inmune en bovinos infectados con tritrichomonas foetus. ...
Journal of Veterinary Diagnostic Investigation, 1995
An immunohistochemical technique using a monoclonal antibody was evaluated as a diagnostic tool t... more An immunohistochemical technique using a monoclonal antibody was evaluated as a diagnostic tool to specifically label Tritrichomonas foetus in formalin-fixed, paraffin-embedded sections of placenta and fetal lung from bovine abortions. Trichomonads were demonstrated in tissues from each of 12 abortions due to T. foetus and none of 15 abortions due to other or unidentified causes. Moderate to marked background staining occurred only in severely autolyzed tissues from T. foetus-infected fetuses. The antibody faintly labeled 1 of 3 other species of trichomonads (Trichomonas gallinae) but did not label other protozoa, bacteria, or fungi tested.
Brazilian Journal of Veterinary Research and Animal Science, 2011
Campylobacter fetus é o agente etiológico da campilobacteriose genital bovina, uma doença sexualm... more Campylobacter fetus é o agente etiológico da campilobacteriose genital bovina, uma doença sexualmente transmissível que está associada com perdas reprodutivas em bovinos. Campylobacter coloniza a vagina e o útero e então infecta as células epiteliais do endométrio. O objetivo deste trabalho foi desenvolver um modelo ex vivo para quantificar a adesão de Campylobacter às células-alvo naturais específicas; este é um passo fundamental para o estabelecimento da infecção e estudos acerca da adesão e citotoxicidade sobre as células do hospedeiro natural não estão disponíveis. Os ensaios foram realizados a través da semeadura de Campylobacter fetus venerealis em culturas celulares epiteliais vaginais e uterinas.Células HeLa foram utilizadas como controle.A aderência bacteriana foi confirmada por microscopia óptica e a determinação da porcentagem de bactérias aderidas foi realizada em lâminas tingidas imunoquimicamente. Os resultados são apresentados como porcentagem de células com Campyloba...
La campylobacteriosis genital bovina es una enfermedad distribuida mundialmente que causa inferti... more La campylobacteriosis genital bovina es una enfermedad distribuida mundialmente que causa infertilidad y aborto. Los mecanismos relacionados con la patogenia de Campylobacter fetus sobre el embrión bovino son aún poco conocidos. Para estudiar el efecto de Campylobacter fetus venerealis en el desarrollo embrionario se utilizaron mórulas y blastocistos murinos. Las mórulas se cultivaron en medio Ham F10 como grupo control (GA; n: 97), más la adición de Campylobacter fetus venerealis (GB; n: 129), adicionado con filtrado libre de células (GC; n: 119) y con caldo Brucella (GD; n: 94). Los blastocistos se cultivaron en medio MEM, grupo control (G1: n: 24), y en MEM más suspensión de Campylobacter fetus venerealis, grupo desafiado (G2; n: 35). A partir de mórulas se evaluaron los porcentajes de diferenciación y hatching. En blastocistos se evaluaron los porcentajes de: hatching, hatched, adhesión y expansión. Los resultados se analizaron mediante Chi². La diferenciación resultó menor a la...
En Argentina, el control de la brucelosis bovina se apoya en la vacunación obligatoria de las ter... more En Argentina, el control de la brucelosis bovina se apoya en la vacunación obligatoria de las terneras con la cepa 19 de Brucella abortus y en la eliminación de los animales seropositivos con destino a faena. Las pruebas serológicas que se emplean más frecuentemente son el BPA (Buffered Plate Antigen) (prueba tamiz) y el FPA (Fluorescence Polarization Assay) (prueba confirmatoria). Ambas detectan anticuerpos anti-cadena O del polisacárido inducidos por la vacunación o por la infección con brucelas de campo. En ocasiones se han encontrado, en un contexto de ausencia de enfermedad clínica, reacciones serológicas atípicas en bovinos adultos. Las mismas han sido atribuidas a la presencia de anticuerpos de reacción cruzada inducidos por vacunas que contienen bacterias Gram negativas. Los objetivos del presente trabajo desarrollado en el modelo murino fueron: estudiar la interferencia en el diagnóstico de la brucelosis bovina generada por la administración de vacunas comerciales que conti...
The interest to develop research on the host-parasite relationship in bovine tritrichomonosis has... more The interest to develop research on the host-parasite relationship in bovine tritrichomonosis has accomplished the use of experimental models alternative to cattle. The BALB/c mouse became the most appropriate species susceptible to vaginal Tritrichomonas foetus infection requiring previous estrogenization. For the need of an experimental model without persistent estrogenization and with normal estrous cycles, the establishment and persistence of vaginal infection on BALB/c mouse with different concentrations of T. foetus in two experimental groups was evaluated. Group A was treated with 5mg of b-estradiol 3-benzoate to synchronize the estrous, 48 hours before the T. foetus vaginal inoculation, and Group B was inoculated in natural estrus. At 5-7 days after treatment, estrogenic effect decreased allowing all animals to cycle regularly during the experiment. From the first week post-infection, samples of vaginal mucus were taken from all animals during 34 weeks, in order to evaluate ...
An experimental murine model of bovine genital tritrichomonosis is described. Female mice were in... more An experimental murine model of bovine genital tritrichomonosis is described. Female mice were inoculated per vaginam with Tritrichomonas foetus and a sample of the study population was killed every 3 days up to 60 days post-infection. Microscopical changes in the reproductive organs were assessed and immunohistochemistry was used to detect T. foetus within these tissues. Lectin histochemistry was used to determine changes in the expression of carbohydrates within the reproductive mucosa. A range of microscopical changes were detected in the uterine endometrium by 10 days post-inoculation and these were associated with the presence of the protozoan. The endometrial changes included endometritis and ulceration, mucosal atrophy and glandular metaplasia, and were similar to those reported in naturally infected cows. Changes in lectin binding were recognized first in the vagina where there was increased binding of Ulex europaeus agglutinin-1 (UEA-1) which was maximal on day 16 post-inoculation. Within the uterus, there was increased binding of soy bean agglutinin (SBA) which was maximal on day 19 post-inoculation, and of peanut agglutinin (PNA) which was maximal on day 16 post-inoculation. These changes in carbohydrate expression parallel the infection kinetics, since they appeared first in the vagina and later in the uterus. The changes may reflect either a host reaction against the infection or the production of enzymes by T. foetus, which act to enhance adhesion and colonization of the genital organs by the organism. The kinetics and pathogenesis of this murine infection are similar to those of the natural bovine disease, suggesting that this model system may be valuable for further studies of this disease.
Universidad Nacional del Centro de la Provincia de Buenos Aires Sistema de Bibliotecas UNICEN Bús... more Universidad Nacional del Centro de la Provincia de Buenos Aires Sistema de Bibliotecas UNICEN Búsqueda general: Formato: Default. ... Autor : Soto, Pedro. Título : La respuesta inmune en bovinos infectados con tritrichomonas foetus. ...
Journal of Veterinary Diagnostic Investigation, 1995
An immunohistochemical technique using a monoclonal antibody was evaluated as a diagnostic tool t... more An immunohistochemical technique using a monoclonal antibody was evaluated as a diagnostic tool to specifically label Tritrichomonas foetus in formalin-fixed, paraffin-embedded sections of placenta and fetal lung from bovine abortions. Trichomonads were demonstrated in tissues from each of 12 abortions due to T. foetus and none of 15 abortions due to other or unidentified causes. Moderate to marked background staining occurred only in severely autolyzed tissues from T. foetus-infected fetuses. The antibody faintly labeled 1 of 3 other species of trichomonads (Trichomonas gallinae) but did not label other protozoa, bacteria, or fungi tested.
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