A histone, macroH2A, nearly three times the size of conventional H2A histone, was found in rat li... more A histone, macroH2A, nearly three times the size of conventional H2A histone, was found in rat liver nucleosomes. Its N-terminal third is 64 percent identical to a full-length mouse H2A. However, it also contains a large nonhistone region. This region has a segment that resembles a leucine zipper, a structure known to be involved in dimerization of some transcription factors. Nucleosomes containing macroH2A may have novel functions, possibly involving interactions with other nuclear proteins.
J. Zlatanova and SH Leuba (Eds.) Chromatin Structure and Dynamics: State-of-the-An C. 2004 Elsevi... more J. Zlatanova and SH Leuba (Eds.) Chromatin Structure and Dynamics: State-of-the-An C. 2004 Elsevier BV All rights reserved DOI ... Thus, the location of the centromere, and CENP-A, could be propagated by an epigenetic mark established by nucleoprotein complexes that ...
MacroH2A is a novel nucleosomal core histone that contains a large nonhistone region and a region... more MacroH2A is a novel nucleosomal core histone that contains a large nonhistone region and a region that closely resembles a full length histone H2A. We have cloned a cDNA that contains the entire coding region of macroH2A1.2, one of the two identified subtypes of macroH2A1. MacroH2A1.2 was found to differ from the other known subtype, macroH2A1.1, in a single segment of the nonhistone region. MacroH2A1 specific antibodies revealed relatively high levels of both subtypes in adult liver and kidney. MacroH2A1.1 was much lower in fetal liver and kidney in comparison to their adult counterparts, and was not detected in adult thymus and testis, tissues with active cell division and differentiation. Both subtypes were present at very low levels or absent from mouse embryonic stem cells maintained in an undifferentiated state by growth in the presence of leukemia inhibitory factor. MacroH2A1.2 increased when the embryonic stem cells were induced to differentiate in vitro, while macroH2A1.1 remained undetectable. These results support the idea that macroH2A1.1 and macroH2A1.2 are functionally distinct, and suggest that changes in their expression may play a role in developmentally regulated changes in chromatin structure and function.
... DEVELOPMENTAL BIOLOGY 111, 530-533 (1985) Distribution of Histone H1a among Cells of the Sea ... more ... DEVELOPMENTAL BIOLOGY 111, 530-533 (1985) Distribution of Histone H1a among Cells of the Sea Urchin Embryo JOHN R. PEHRSON AND LEONARD H ... This hypothesis predicts that in the late embryo, Hies and Hia will be abundant in the chromatin of di-viding cells, and ...
We show that in sea urchin embryos, the daughter cells of the small micromeres become part of the... more We show that in sea urchin embryos, the daughter cells of the small micromeres become part of the coelomic sacs, in contrast to the long-held view that these sacs are purely of macromere origin. In addition, after prolonged mitotic quiescence, and following their incorporation into the coelomic sacs, these cells resume dividing, contrary to the previous view that they do not divide. Since coelomic sac cells give rise to much of the adult urchin, our results indicate that the small micromeres are founders of cell lineages involved in the formation of adult tissues. The setting aside of these cells in a nondividing state may be analogous to a phenomenon in Drosophila development, in which primordial imaginal and germ cells divide approximately once after the blastoderm stage and do not resume dividing until the larval stage.
A histone, macroH2A, nearly three times the size of conventional H2A histone, was found in rat li... more A histone, macroH2A, nearly three times the size of conventional H2A histone, was found in rat liver nucleosomes. Its N-terminal third is 64 percent identical to a full-length mouse H2A. However, it also contains a large nonhistone region. This region has a segment that resembles a leucine zipper, a structure known to be involved in dimerization of some transcription factors. Nucleosomes containing macroH2A may have novel functions, possibly involving interactions with other nuclear proteins.
J. Zlatanova and SH Leuba (Eds.) Chromatin Structure and Dynamics: State-of-the-An C. 2004 Elsevi... more J. Zlatanova and SH Leuba (Eds.) Chromatin Structure and Dynamics: State-of-the-An C. 2004 Elsevier BV All rights reserved DOI ... Thus, the location of the centromere, and CENP-A, could be propagated by an epigenetic mark established by nucleoprotein complexes that ...
MacroH2A is a novel nucleosomal core histone that contains a large nonhistone region and a region... more MacroH2A is a novel nucleosomal core histone that contains a large nonhistone region and a region that closely resembles a full length histone H2A. We have cloned a cDNA that contains the entire coding region of macroH2A1.2, one of the two identified subtypes of macroH2A1. MacroH2A1.2 was found to differ from the other known subtype, macroH2A1.1, in a single segment of the nonhistone region. MacroH2A1 specific antibodies revealed relatively high levels of both subtypes in adult liver and kidney. MacroH2A1.1 was much lower in fetal liver and kidney in comparison to their adult counterparts, and was not detected in adult thymus and testis, tissues with active cell division and differentiation. Both subtypes were present at very low levels or absent from mouse embryonic stem cells maintained in an undifferentiated state by growth in the presence of leukemia inhibitory factor. MacroH2A1.2 increased when the embryonic stem cells were induced to differentiate in vitro, while macroH2A1.1 remained undetectable. These results support the idea that macroH2A1.1 and macroH2A1.2 are functionally distinct, and suggest that changes in their expression may play a role in developmentally regulated changes in chromatin structure and function.
... DEVELOPMENTAL BIOLOGY 111, 530-533 (1985) Distribution of Histone H1a among Cells of the Sea ... more ... DEVELOPMENTAL BIOLOGY 111, 530-533 (1985) Distribution of Histone H1a among Cells of the Sea Urchin Embryo JOHN R. PEHRSON AND LEONARD H ... This hypothesis predicts that in the late embryo, Hies and Hia will be abundant in the chromatin of di-viding cells, and ...
We show that in sea urchin embryos, the daughter cells of the small micromeres become part of the... more We show that in sea urchin embryos, the daughter cells of the small micromeres become part of the coelomic sacs, in contrast to the long-held view that these sacs are purely of macromere origin. In addition, after prolonged mitotic quiescence, and following their incorporation into the coelomic sacs, these cells resume dividing, contrary to the previous view that they do not divide. Since coelomic sac cells give rise to much of the adult urchin, our results indicate that the small micromeres are founders of cell lineages involved in the formation of adult tissues. The setting aside of these cells in a nondividing state may be analogous to a phenomenon in Drosophila development, in which primordial imaginal and germ cells divide approximately once after the blastoderm stage and do not resume dividing until the larval stage.
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