Additional file 3: Data S1A. Genome sequencing and analysis of L. lactis 9k-5A. (A) Genome sequen... more Additional file 3: Data S1A. Genome sequencing and analysis of L. lactis 9k-5A. (A) Genome sequencing of L. lactis 9k-5A.
Additional file 5: Data S1B. Genome sequencing and analysis of L. lactis 9k-5A. (B) Arrangement o... more Additional file 5: Data S1B. Genome sequencing and analysis of L. lactis 9k-5A. (B) Arrangement of 66 large scaffolds in L. lactis 9k-5A.
The cellulose-binding domain (CBD) of XylA was fused with PrtP, NisP and AcmA anchors derived fro... more The cellulose-binding domain (CBD) of XylA was fused with PrtP, NisP and AcmA anchors derived from Lactococcus lactis under P45 promoter and Usp45 secretion signal. The fusion construct with the anchor PrtP (334 aa) was shown to exhibit the most efficient immobilization. The CBD-PrtP construct on the other hand was not efficiently attached to the cell wall and as such was found mainly in the supernatant. Results also showed that expression of the CBD-NisP anchor fusion led to a similar result. This raised the question if more efficient binding of the anchor to the cell wall by sortase could enhance the efficiency of cell immobilization to the cellulosic material. However, expressing sortase with the CBD-PrtP fusion did not improve the immobilization of the cells to cellulose.
Endophytic fungi and bacteria were isolated from finger millet and their effects on finger millet... more Endophytic fungi and bacteria were isolated from finger millet and their effects on finger millet growth parameters and zinc and NPK contents in grains were studied. Out of 70 fungal and 112 bacterial endophytes, the two best fungal and bacterial isolates were selected on the basis of zinc solubilization and plant-growth-promoting attributes. The fungal isolates identified were Aspergillus terreus and Lecanicillium sp., and the bacterial isolates were Pseudomonas bijieensis and Priestia megaterium. The endophytic zinc, NPK mobilization, and plant-growth-promoting efficacy were determined in a pot experiment with zinc carbonate as the zinc source. Endophytic-primed plants showed enhanced shoot and root lengths compared to the unprimed control. Endophytes increased the zinc content in grains by between 12.12% and 18.80% compared to control plants. Endophytes also augmented the NPK concentrations in seeds compared to control plants and exhibited stability in a diverse range of pHs, tem...
This study aims to explore how feuD mutation triggered the increase in nisin immunity of Lactococ... more This study aims to explore how feuD mutation triggered the increase in nisin immunity of Lactococcus lactis L58, which was proven to be a feuD::Em-Mu mutant of L. lactis N8. The significant difference genes of L. lactis L58 and L. lactis N8 were compared at transcription and protein levels. Analysis revealed that the feuD mutation induced the decrease of histidine-containing phosphocarrier protein PtsH (HPr) and the increase of thioredoxin reductase TrxB (TR). Determination of iron concentration and cytoplasmic membrane potential (MP) showed the iron concentration decreased around 10% and the MP decreased approximately 14% in L. lactis L58. The increase of nisin immunity was dominated by TR up-expression by two main mechanisms in L. lactis L58. First, the TR-TRX (thioredoxin reductase) system changed the composition of cytoplasmic membrane by regulating the lipid metabolism to enhance the cells' resistance to nisin. Second, iron starvation stress induced the decrease of MP; hence, the binding affinity of nisin to lipid II of L. lactis L58 decreased, which, in turn, increased the nisin immunity. The knowledge on regulation mechanism of nisin immunity was enriched, and the theoretical basis for improving nisin production in engineering strain could be provided. This article is protected by copyright. All rights reserved.
Additional file 3: Data S1A. Genome sequencing and analysis of L. lactis 9k-5A. (A) Genome sequen... more Additional file 3: Data S1A. Genome sequencing and analysis of L. lactis 9k-5A. (A) Genome sequencing of L. lactis 9k-5A.
Additional file 5: Data S1B. Genome sequencing and analysis of L. lactis 9k-5A. (B) Arrangement o... more Additional file 5: Data S1B. Genome sequencing and analysis of L. lactis 9k-5A. (B) Arrangement of 66 large scaffolds in L. lactis 9k-5A.
The cellulose-binding domain (CBD) of XylA was fused with PrtP, NisP and AcmA anchors derived fro... more The cellulose-binding domain (CBD) of XylA was fused with PrtP, NisP and AcmA anchors derived from Lactococcus lactis under P45 promoter and Usp45 secretion signal. The fusion construct with the anchor PrtP (334 aa) was shown to exhibit the most efficient immobilization. The CBD-PrtP construct on the other hand was not efficiently attached to the cell wall and as such was found mainly in the supernatant. Results also showed that expression of the CBD-NisP anchor fusion led to a similar result. This raised the question if more efficient binding of the anchor to the cell wall by sortase could enhance the efficiency of cell immobilization to the cellulosic material. However, expressing sortase with the CBD-PrtP fusion did not improve the immobilization of the cells to cellulose.
Endophytic fungi and bacteria were isolated from finger millet and their effects on finger millet... more Endophytic fungi and bacteria were isolated from finger millet and their effects on finger millet growth parameters and zinc and NPK contents in grains were studied. Out of 70 fungal and 112 bacterial endophytes, the two best fungal and bacterial isolates were selected on the basis of zinc solubilization and plant-growth-promoting attributes. The fungal isolates identified were Aspergillus terreus and Lecanicillium sp., and the bacterial isolates were Pseudomonas bijieensis and Priestia megaterium. The endophytic zinc, NPK mobilization, and plant-growth-promoting efficacy were determined in a pot experiment with zinc carbonate as the zinc source. Endophytic-primed plants showed enhanced shoot and root lengths compared to the unprimed control. Endophytes increased the zinc content in grains by between 12.12% and 18.80% compared to control plants. Endophytes also augmented the NPK concentrations in seeds compared to control plants and exhibited stability in a diverse range of pHs, tem...
This study aims to explore how feuD mutation triggered the increase in nisin immunity of Lactococ... more This study aims to explore how feuD mutation triggered the increase in nisin immunity of Lactococcus lactis L58, which was proven to be a feuD::Em-Mu mutant of L. lactis N8. The significant difference genes of L. lactis L58 and L. lactis N8 were compared at transcription and protein levels. Analysis revealed that the feuD mutation induced the decrease of histidine-containing phosphocarrier protein PtsH (HPr) and the increase of thioredoxin reductase TrxB (TR). Determination of iron concentration and cytoplasmic membrane potential (MP) showed the iron concentration decreased around 10% and the MP decreased approximately 14% in L. lactis L58. The increase of nisin immunity was dominated by TR up-expression by two main mechanisms in L. lactis L58. First, the TR-TRX (thioredoxin reductase) system changed the composition of cytoplasmic membrane by regulating the lipid metabolism to enhance the cells' resistance to nisin. Second, iron starvation stress induced the decrease of MP; hence, the binding affinity of nisin to lipid II of L. lactis L58 decreased, which, in turn, increased the nisin immunity. The knowledge on regulation mechanism of nisin immunity was enriched, and the theoretical basis for improving nisin production in engineering strain could be provided. This article is protected by copyright. All rights reserved.
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