Acute splenic sequestration crisis is a rare disorder that usually occurs in children, with sickl... more Acute splenic sequestration crisis is a rare disorder that usually occurs in children, with sickle cell anemia, who are under the age of five years. A few cases have been described in adults with heterozygous sickle cell syndromes. Though this entity can be fatal there have been no reported cases associated with sudden death. We describe a case of sudden, unexpected death, associated with splenic sequestration, in a 29-year-old African-American man with undiagnosed sickle cell-beta-thalassemia syndrome.
Ultrahigh-resolution optical coherence tomography (OCT) has an axial resolution of &a... more Ultrahigh-resolution optical coherence tomography (OCT) has an axial resolution of <5 microm, 2 to 3 times finer than standard OCT. This study investigates ultrahigh-resolution and three-dimensional OCT for ex vivo imaging of the large and small intestines and correlates images with histology. Ultrahigh-resolution OCT imaging was performed on fresh surgical specimens from the large and small intestines in the pathology laboratory, and images were correlated with histology. OCT was performed at 1.3-microm wavelength with 4.5-microm axial x 11-microm transverse resolution and at 1.1-microm wavelength with 3.5-microm axial x 6-microm transverse resolution. Three-dimensional OCT also was investigated. Normal and pathologic areas from 23 surgical specimens of the large and small intestines were imaged. Ultrahigh-resolution OCT distinguished the epithelial layer of the mucosa and visualized individual villi, glands, and crypts. Finer transverse resolutions improved visualization of features, e.g., the epithelium, but reduced the depth of field. Architectural distortion of glands from inflammatory and neoplastic processes was observed. Three-dimensional rendering enabled visualization of surface pit pattern and mucosal folds as well as subsurface crypt microstructure. This study evaluates new OCT technology and can provide a baseline for interpreting future ultrahigh-resolution endoscopic OCT studies.
Institutional review board approval at the participating institutions was obtained. Informed cons... more Institutional review board approval at the participating institutions was obtained. Informed consent was waived for this HIPAA-compliant study. The study purpose was to establish the correspondence of optical coherence tomographic (OCT) image findings with histopathologic findings to understand which features characteristic of breast lesions can be visualized with OCT. Imaging was performed in 119 specimens from 35 women aged 29-81 years with 3.5-microm axial resolution and 6-microm transverse resolution at 1.1-microm wavelength on freshly excised specimens of human breast tissue. Three-dimensional imaging was performed in 43 specimens from 23 patients. Microstructure of normal breast parenchyma, including glands, lobules, and lactiferous ducts, and stromal changes associated with infiltrating cancer were visible. Fibrocystic changes and benign fibroadenomas were identified. Imaging of ductal carcinoma in situ, infiltrating cancer, and microcalcifications correlated with corresponding histopathologic findings. OCT is potentially useful for visualization of breast lesions at a resolution greater than that of currently available clinical imaging methods.
Acute splenic sequestration crisis is a rare disorder that usually occurs in children, with sickl... more Acute splenic sequestration crisis is a rare disorder that usually occurs in children, with sickle cell anemia, who are under the age of five years. A few cases have been described in adults with heterozygous sickle cell syndromes. Though this entity can be fatal there have been no reported cases associated with sudden death. We describe a case of sudden, unexpected death, associated with splenic sequestration, in a 29-year-old African-American man with undiagnosed sickle cell-beta-thalassemia syndrome.
Ultrahigh-resolution optical coherence tomography (OCT) has an axial resolution of &a... more Ultrahigh-resolution optical coherence tomography (OCT) has an axial resolution of <5 microm, 2 to 3 times finer than standard OCT. This study investigates ultrahigh-resolution and three-dimensional OCT for ex vivo imaging of the large and small intestines and correlates images with histology. Ultrahigh-resolution OCT imaging was performed on fresh surgical specimens from the large and small intestines in the pathology laboratory, and images were correlated with histology. OCT was performed at 1.3-microm wavelength with 4.5-microm axial x 11-microm transverse resolution and at 1.1-microm wavelength with 3.5-microm axial x 6-microm transverse resolution. Three-dimensional OCT also was investigated. Normal and pathologic areas from 23 surgical specimens of the large and small intestines were imaged. Ultrahigh-resolution OCT distinguished the epithelial layer of the mucosa and visualized individual villi, glands, and crypts. Finer transverse resolutions improved visualization of features, e.g., the epithelium, but reduced the depth of field. Architectural distortion of glands from inflammatory and neoplastic processes was observed. Three-dimensional rendering enabled visualization of surface pit pattern and mucosal folds as well as subsurface crypt microstructure. This study evaluates new OCT technology and can provide a baseline for interpreting future ultrahigh-resolution endoscopic OCT studies.
Institutional review board approval at the participating institutions was obtained. Informed cons... more Institutional review board approval at the participating institutions was obtained. Informed consent was waived for this HIPAA-compliant study. The study purpose was to establish the correspondence of optical coherence tomographic (OCT) image findings with histopathologic findings to understand which features characteristic of breast lesions can be visualized with OCT. Imaging was performed in 119 specimens from 35 women aged 29-81 years with 3.5-microm axial resolution and 6-microm transverse resolution at 1.1-microm wavelength on freshly excised specimens of human breast tissue. Three-dimensional imaging was performed in 43 specimens from 23 patients. Microstructure of normal breast parenchyma, including glands, lobules, and lactiferous ducts, and stromal changes associated with infiltrating cancer were visible. Fibrocystic changes and benign fibroadenomas were identified. Imaging of ductal carcinoma in situ, infiltrating cancer, and microcalcifications correlated with corresponding histopathologic findings. OCT is potentially useful for visualization of breast lesions at a resolution greater than that of currently available clinical imaging methods.
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Papers by Darshan Phatak