Flagella-templated Au nanowires were fabricated and demonstrated superior electrocatalytic activi... more Flagella-templated Au nanowires were fabricated and demonstrated superior electrocatalytic activity in the inhibition of bacterial growth by plant secondary metabolites.
Foods that are rich in protein and other micronutrients such as bioactives and vitamins but low i... more Foods that are rich in protein and other micronutrients such as bioactives and vitamins but low in sugar and fat may help mitigate the global obesity epidemic. Specialty ingredients can be fabricated to possess specific functions such as encapsulating bioactives through controlled assembly of protein and polysaccharide molecules. One of the most commonly used methods of combining proteins and polysaccharides into functional biopolymer particles is based on electrostatic attraction between oppositely charged groups under appropriate conditions(concentration, pH, ionic strength, and temperature etc.). In this work, interactions betweenβ-lactoglobulin(β-LG), a major milk protein, and sugar beet pectin(SBP), one of the most versatile polysaccharides, were investigated using online multi-detection High Performance Size Exclusion Chromatography(HPSEC) at neutral pH. The hydrodynamic properties of various interacting polymeric fractions were characterized in detail. Results showed that alt...
Abstract We investigated the molecular properties of interacting complexes and conjugates formed ... more Abstract We investigated the molecular properties of interacting complexes and conjugates formed between α-lactalbumin (αLA) and SBP using a multi-detection (UV, dRI, MALLS, and DPV) HPSEC. Dry-heating reactions were conducted at the mass ratios 1:0, 5:1, 3:1, 2:1, 1:1, and 0:1 αLA:SBP under controlled conditions (60 °C, 79% RH, 72 h). The in-depth physicochemical analysis showed that the association levels were 32, 37, 47, and 61% αLA and 18, 24, 30, and 42% SBP for 5:1, 3:1, 2:1, and 1:1 αLA:SBP, respectively. The stoichiometry was estimated to be 233, 121, 90, and 45 αLA per SBP. Studies of the solution mixtures (3:1) demonstrated that αLA formed complexes with all sized SBP at pH ~ 6.50 through local charge-charge and hydrophobic interactions with stoichiometry ~110:1. Furthermore, the complexes and conjugates did not change the conformation of SBP considerably, i.e., compact spheres, random coils, and rigid rods but increased the molecular chains' stiffness.
Olive leaf extract (OLE) has been increasingly recognized as a natural and effective antimicrobia... more Olive leaf extract (OLE) has been increasingly recognized as a natural and effective antimicrobial against a host of foodborne pathogens. This study attempts to predict the minimum inhibitory concentration (MIC) of OLE against Listeria monocytogenes F2365 by utilizing the asymptotic deceleration point (PDA) in a logistic model (LM), namely MIC-PDA. The experimental data obtained from the inhibitory rate (IR) versus OLE concentration against L. monocytogenes were sufficiently fitted (R2 = 0.88957). Five significant critical points were derived by taking the multi-order derivatives of the LM function: the inflection point (PI), the maximum acceleration point (PAM), the maximum deceleration point (PDM), the absolute acceleration point (PAA), and the asymptotic deceleration point (PDA). The PDA ([OLE] = 37.055 mg/mL) was employed to approximate the MIC-PDA. This MIC value was decreased by over 42% compared to the experimental MIC of 64.0 mg/mL, obtained using the conventional 2-fold dil...
Research and development on films and coatings made from various agricultural proteins has been c... more Research and development on films and coatings made from various agricultural proteins has been conducted over the past 20 years, but is of heightened interest, due to the demand for environmentally-friendly, renewable replacements for petroleumbased polymeric materials and plastics. To address this demand, films and coatings have been made from renewable resources, such as casein, whey, soy, corn zein, collagen, wheat gluten, keratin and egg albumen. Those made from agricultural proteins create new outlets for agricultural products, byproducts and waste streams, all of which can positively impact the economics of food processes. Due to casein's ability to form water-resistant films, it was used for hundreds of years in paints and coatings (Gettens and Stout 1984). In the late nineteenth century, casein was converted into a hard plastic material by cross-linking it with formaldehyde. A patent for this technology was issued to Adolf Spitteler in Bavaria (Brother 1940), and it was...
Flagella-templated Au nanowires were fabricated and demonstrated superior electrocatalytic activi... more Flagella-templated Au nanowires were fabricated and demonstrated superior electrocatalytic activity in the inhibition of bacterial growth by plant secondary metabolites.
Foods that are rich in protein and other micronutrients such as bioactives and vitamins but low i... more Foods that are rich in protein and other micronutrients such as bioactives and vitamins but low in sugar and fat may help mitigate the global obesity epidemic. Specialty ingredients can be fabricated to possess specific functions such as encapsulating bioactives through controlled assembly of protein and polysaccharide molecules. One of the most commonly used methods of combining proteins and polysaccharides into functional biopolymer particles is based on electrostatic attraction between oppositely charged groups under appropriate conditions(concentration, pH, ionic strength, and temperature etc.). In this work, interactions betweenβ-lactoglobulin(β-LG), a major milk protein, and sugar beet pectin(SBP), one of the most versatile polysaccharides, were investigated using online multi-detection High Performance Size Exclusion Chromatography(HPSEC) at neutral pH. The hydrodynamic properties of various interacting polymeric fractions were characterized in detail. Results showed that alt...
Abstract We investigated the molecular properties of interacting complexes and conjugates formed ... more Abstract We investigated the molecular properties of interacting complexes and conjugates formed between α-lactalbumin (αLA) and SBP using a multi-detection (UV, dRI, MALLS, and DPV) HPSEC. Dry-heating reactions were conducted at the mass ratios 1:0, 5:1, 3:1, 2:1, 1:1, and 0:1 αLA:SBP under controlled conditions (60 °C, 79% RH, 72 h). The in-depth physicochemical analysis showed that the association levels were 32, 37, 47, and 61% αLA and 18, 24, 30, and 42% SBP for 5:1, 3:1, 2:1, and 1:1 αLA:SBP, respectively. The stoichiometry was estimated to be 233, 121, 90, and 45 αLA per SBP. Studies of the solution mixtures (3:1) demonstrated that αLA formed complexes with all sized SBP at pH ~ 6.50 through local charge-charge and hydrophobic interactions with stoichiometry ~110:1. Furthermore, the complexes and conjugates did not change the conformation of SBP considerably, i.e., compact spheres, random coils, and rigid rods but increased the molecular chains' stiffness.
Olive leaf extract (OLE) has been increasingly recognized as a natural and effective antimicrobia... more Olive leaf extract (OLE) has been increasingly recognized as a natural and effective antimicrobial against a host of foodborne pathogens. This study attempts to predict the minimum inhibitory concentration (MIC) of OLE against Listeria monocytogenes F2365 by utilizing the asymptotic deceleration point (PDA) in a logistic model (LM), namely MIC-PDA. The experimental data obtained from the inhibitory rate (IR) versus OLE concentration against L. monocytogenes were sufficiently fitted (R2 = 0.88957). Five significant critical points were derived by taking the multi-order derivatives of the LM function: the inflection point (PI), the maximum acceleration point (PAM), the maximum deceleration point (PDM), the absolute acceleration point (PAA), and the asymptotic deceleration point (PDA). The PDA ([OLE] = 37.055 mg/mL) was employed to approximate the MIC-PDA. This MIC value was decreased by over 42% compared to the experimental MIC of 64.0 mg/mL, obtained using the conventional 2-fold dil...
Research and development on films and coatings made from various agricultural proteins has been c... more Research and development on films and coatings made from various agricultural proteins has been conducted over the past 20 years, but is of heightened interest, due to the demand for environmentally-friendly, renewable replacements for petroleumbased polymeric materials and plastics. To address this demand, films and coatings have been made from renewable resources, such as casein, whey, soy, corn zein, collagen, wheat gluten, keratin and egg albumen. Those made from agricultural proteins create new outlets for agricultural products, byproducts and waste streams, all of which can positively impact the economics of food processes. Due to casein's ability to form water-resistant films, it was used for hundreds of years in paints and coatings (Gettens and Stout 1984). In the late nineteenth century, casein was converted into a hard plastic material by cross-linking it with formaldehyde. A patent for this technology was issued to Adolf Spitteler in Bavaria (Brother 1940), and it was...
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