The present work describes a reversed phase high performance liquid chromatographic method for si... more The present work describes a reversed phase high performance liquid chromatographic method for simultaneous estimation of Citicoline and Piracetam in tablet dosage form. The estimation was carried out on a C18 column using a mixture of acetonitrile and 10 mM disodium hydrogen phosphate buffer in the ratio of 10:90 % v/v as a mobile phase. The pH of aqueous phase was adjusted to 6.0 with 1 % o-phosphoric acid. The flow rate of mobile phase was maintained at 1.0 mL/min. To achieve highest precision in the analysis, Paracetamol was used as an internal standard. All analytes were detected by measuring the absorbance at 210 nm. Total run time was 10 min; Citicoline, Piracetam and Paracetamol were eluted at the retention times of 2.6, 3.5 and 5.6 min respectively. Calibration curves were found linear over the concentration ranges of 10-100 µg/mL for Citicoline and 16-160 µg/mL for Piracetam. The method was validated for accuracy, precision, linearity, specificity and sensitivity as per IC...
This study proposes a simple, specific, precise and accurate spectrophotometric method for estima... more This study proposes a simple, specific, precise and accurate spectrophotometric method for estimation of Citicoline sodium in bulk and tablet dosage form. The results presented are statistically validated in accordance with the guidelines provided by ICH (International conference on harmonization). The solvents used were 0.1N sodium hydroxide and double distilled water. With an absorption maxima of 272 nm, the drug followed a linear relationship in the range of 5-55μg/ml while the correlation coefficient was at 0.9983. The recovery was 99.90% (+0.50) and the coefficient of variance was found to be less than 5%.
In the present study, spectrophotometric method was developed and validated using multicomponent ... more In the present study, spectrophotometric method was developed and validated using multicomponent mode for simultaneous estimation of Salbutamol Sulphate and Theophylline in combined tablet dosage form. Distilled water was used as a solvent. Considering the absorption of both the analytes in the range of 200 nm -320 nm, four wavelength of equal interval of 40 nm were selected such as 320 nm, 280 nm, 240 nm and 200 nm for analysis of these drugs. The mean percentage assay was found as 100.50% and 100.79% for Salbutamol Sulphate and Theophylline respectively. Results of analysis were validated statistically in accordance with ICH guidelines for accuracy and precision of the method. The results of recovery study were observed in the range of 93.26–99.24%. for Salbutamol Sulphate and 97.39–103.615% for Theophylline, While, the % RSD for precision study was observed less than 1%. So, it can be concluded that, the developed method is simple, accurate, economical and easy to apply, making i...
Asian Journal of Pharmaceutical and Clinical Research, 2014
Objective – To develop and validate RP-HPLC method for estimation of Pimozide in bulk and its tab... more Objective – To develop and validate RP-HPLC method for estimation of Pimozide in bulk and its tablet dosage form. Method- Chromatographic separation was carried out on Grace Smart RP-18 column (250 mm X 4.6 mm inner diameter; 5µm particle size) using a mobile phase consisted of Acetonitrile: 50 mM Disodium hydrogen phosphate buffer (pH 6.2, adjusted by 1 % ortho phosphoric acid) in the ratio of 60: 40 %v/v. The flow rate was maintained at 1 ml/min and UV detection was measured at 280 nm. Propyphenazone was used as an internal standard. Results-The calibration curve of Pimozide was linear in range of 5- 100 µg/ml. The mean % assay of marketed formulation was found to be 101.02 % and % recovery was observed in the range of 99.23- 101.91 %. Relative standard deviation for precision study was found less than 2 %. The LOD and LOQ values were found to be 0.553 µg/ml and 1.678 µg/ml respectively. Conclusion- The developed method is simple rapid and easy to apply, making it suitable for rou...
In the present research work, absorbance correction method and first order derivative spectrophot... more In the present research work, absorbance correction method and first order derivative spectrophotometric method have been developed for the simultaneous estimation of Metaxalone and Diclofenac potassium in tablet dosage form. Methanol was used as a solvent in both the methods. The wavelengths of absorption of Metaxalone and Diclofenac potassium were found to be 279 nm and 300 nm respectively and absorbencies at theses wavelengths were used for absorption correction method, while in the first order derivative spectrophotometric method, amplitudes were measured at 302.42nm for Metaxalone (zero cross for Diclofenac potassium) and 282.08 nm for Diclofenac potassium (zero cross for Metaxalone). Metaxalone was found to be linear in the concentration range of 20 to 180µg/ml at 279nm while Diclofenac potassium was found to be linear in the concentration range 5 to 35µg/ml at 300nm and 3 to 24 µg/ml at 279nm. The marketed formulation was analysed using these methods and the results were obta...
A simple, sensitive and specific UV spectrophotometric method has been proposed for the estimatio... more A simple, sensitive and specific UV spectrophotometric method has been proposed for the estimation of Isotretinoin in bulk and soft gelatin capsule for routine analysis. The optimum conditions for analysis were established and validated in conformance with ICH guidelines. It was observed, the absorbance maximum (λmax) for Isotretinoin was 344 nm in methanol and the linearity was in the range of 1-8 μg/ml with coefficient of correlation as 0.9994. The lower limit of detection and the limit of quantification were found to be 0.2519 and 0.7634μg/ml respectively.
In the present study, a reverse phase high performance liquid chromatographic method was develope... more In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Rasagiline in tablet dosage form with the use of Caffeine as an internal standard. Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile: isopropyl alcohol: water (10:20:70, v/v/v) adjusted at pH 2.5 with o-phosphoric acid (1%). The flow rate was maintained at 0.8 ml min−1 and UV detection was measured at 210 nm. The calibration curve was linear over the range 1–200μgml−1. R.S.D. for precision was <3%. The results of accuracy study was observed in the range of 95.16% to 102.24% with R.S.D. < 3%. LOD and LOQ were found to be 0.4044μgml−1 and 1.2133μgml−1, respectively. The method was simple, rapid, and easy to apply, making it suitable for routine analysis of Rasagiline in tablet dosage form.
A Multicomponent Spectrophotometric method for simultaneous estimation of Ketotifen fumarate and ... more A Multicomponent Spectrophotometric method for simultaneous estimation of Ketotifen fumarate and Salbutamol sulphate in combined tablet dosage form has been proposed. Considering the absorption spectra of both analytes in the range of 220-360 nm in water, the four wavelengths of equal intervals of 40 nm were selected as 350 nm, 320 nm, 280 nm and 240 nm to produce calibration curves of mixture of analytes. The present method used inbuilt application of instrument (Shimadzu Pharm Spec 1700, UV-Visible Spectrophotometer) to quantify Ketotifen fumarate and Salbutamol sulphate in formulation. The mean % assay was found as 102.90% and 101.05% for Ketotifen fumarate and Salbutamol sulphate respectively. The method has been validated in accordance with ICH guidelines for accuracy and precision. The mean % recovery was found in range of 98.85 to 99.62% for Ketotifen fumarate and 99.08 to 100.65 % for Salbutamol sulphate, while % RSD was observed as less than 1% for precision study. The deve...
In present research work, a simple Multicomponent Spectrophotometric method for simultaneous esti... more In present research work, a simple Multicomponent Spectrophotometric method for simultaneous estimation of Ketotifen fumarate and Cetirizine dihydrochloride in pharmaceutical dosage was developed. The method used is inbuilt application of instrument (Shimadzu Pharm Spec 1700, UV spectrophotometer) to quantify Ketotifen fumarate and Cetirizine dihydrochloride in formulation. Considering the absorption of both analytes in the range of 200-360 nmin water, the wavelength intervals of 40 nmwas selected such as 360 nm, 320 nm, 280 nm, 240 nm and 200 nm for measurements. The serial dilutions of standard were scanned and absorptions were stored and sample concentration was determined by the instrument. The mean assay was found as about 100.3 %. The method has been validated in accordancewith ICH guidelines for accuracy and precision. Result of recovery study was observed in the range of 98.78% to 101.48% forKetotifen fumarate and 98.77%to 101.48%forCetirizine dihydrochloride. The % RSD for ...
Two methods for simultaneous estimation of Aspirin and Ticlopidine hydrochloride in combination h... more Two methods for simultaneous estimation of Aspirin and Ticlopidine hydrochloride in combination have been developed using 0.1N hydrochloric acid as a solvent. The sample solution was scanned in the range 190-400nm at fast speed. The resulted spectrum was converted into first and second order derivative (using A e=16, scaling factor 100). In second order derivative spectroscopy Aspirin was measured at 303nm while Ticlopidine hydrochloride was determined at 288nm. In second method, amplitudes of first order derivative were measured at 279.2 and 288.2nm and the concentration of Ticlopidine andAspirin were estimated by using simultaneous equation method. The linearity ranges for Aspirin and Ticlopidine hydrochloride were found to be 20-200µg/ml and 100- 700µg/ml respectively at 279.2nm, 288.2nm for first order and 288nm, 303nm for second order derivative method. The accuracy of the methods was assessed by recovery studies was found to be 95.95% and 101.49% for second order derivative me...
Journal of Analytical & Bioanalytical Techniques, 2012
A simple Multicomponent Spectrophotometric method for simultaneous estimation of Metaxalone and D... more A simple Multicomponent Spectrophotometric method for simultaneous estimation of Metaxalone and Diclofenac Potassium in combined tablet dosage form had been proposed. Considering the absorption spectra of both analytes in the range of 220-350 nm in methanol, the four wavelengths of equal intervals of 40 nm were selected as 350 nm, 310 nm, 270 nm and 230 nm to produce calibration curves of mixture of analytes. The present method used inbuilt application of instrument (Shimadzu Pharm Spec 1700, UV spectrophotometer) to quantify Metaxalone and Diclofence potassium in formulation. The method had been validated in accordance with ICH guidelines for accuracy and precision.
In the present study, a reverse phase high performance liquid chromatographic method was develope... more In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Citicoline sodium in pharmaceutical dosage form. Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile: water (20:80, v/v) adjusted at pH 3.0 using 1% orthophosphoric acid. Flow rate was maintained at 0.7ml min and UV detection was carried at 260 nm. Caffeine was used as an internal standard. The calibration curve was found to be linear over the range 1–500μgml. The results of accuracy study were ranged between 99.32% and 101.90% with a R.S.D. less than 1%. LOD and LOQ were 1.4μgml and 4.3μgml, respectively. The method was found to be simple, rapid, and easy to apply, making it very suitable for routine analysis of Citicoline sodium in pharmaceutical dosage form.
In the present study, a reverse phase high performance liquid chromatographic method was develope... more In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Diacerein (DIA) andAceclofenac (ACE) in tablet dosage formwith the use of Paracetamol (PCM) as an internal standard (IS). Chromatographic separation was carried out on a RP-18 column using amobile phase consisting of acetonitrile : water (60:40, v/v) 0f pH 3.0,adjusted with o-phosphoric acid (1%). The flow rate was maintained at 1.0ml/min and UV detection was carried at 268nm. The calibration curve was found linear over the range 10-150µg/ml for DIA and 20-300µg/ml. Relative Standard deviation (RSD) for precision was found to be less than 3%. The results of accuracy study were observed in the range of 99.50%to100.18%withRSD less than 1%. Limit of detection (LOD)was found to be 0.9839µg/ml for DIA and 1.9479µg/ml for ACE. Limit of quantification (LOQ)was found to be 2.9816µg/ml for DIA and 5.9030µg/ ml forACE. The developed analyticalmethod was fou...
A specific, accurate, precise and sensitive validated reverse phase liquid chromatographic (RP-HP... more A specific, accurate, precise and sensitive validated reverse phase liquid chromatographic (RP-HPLC) method has been developed for the simultaneous estimation of Ofloxacin, Ornidazole and its isomer in bulk drug as well as tablet dosage form. Drugs were analysed on C18 column using mobile phase acetonitrile: methanol: 0.025M phosphate buffer (pH 3.0) (30:10:60 v/v/v). A flow rate was maintained at 1.0 ml/min and detection was made at 318 nm. The retention time for Ofloxacin, Ornidazole and its isomer was found to be 4.04, 5.82 and 6.77 min respectively. Proposed method was validated for accuracy, precision, linearity and range, ruggedness. Linearity of Ofloxacin and Ornidazole was in the range of 2–40 μg/ml and 5–100 μg/ml respectively. Average percentage recoveries obtained for Ofloxacin and Ornidazole were 100.20% and 100.9%.
The present work describes a reversed phase high performance liquid chromatographic method for si... more The present work describes a reversed phase high performance liquid chromatographic method for simultaneous estimation of Citicoline and Piracetam in tablet dosage form. The estimation was carried out on a C18 column using a mixture of acetonitrile and 10 mM disodium hydrogen phosphate buffer in the ratio of 10:90 % v/v as a mobile phase. The pH of aqueous phase was adjusted to 6.0 with 1 % o-phosphoric acid. The flow rate of mobile phase was maintained at 1.0 mL/min. To achieve highest precision in the analysis, Paracetamol was used as an internal standard. All analytes were detected by measuring the absorbance at 210 nm. Total run time was 10 min; Citicoline, Piracetam and Paracetamol were eluted at the retention times of 2.6, 3.5 and 5.6 min respectively. Calibration curves were found linear over the concentration ranges of 10-100 µg/mL for Citicoline and 16-160 µg/mL for Piracetam. The method was validated for accuracy, precision, linearity, specificity and sensitivity as per IC...
This study proposes a simple, specific, precise and accurate spectrophotometric method for estima... more This study proposes a simple, specific, precise and accurate spectrophotometric method for estimation of Citicoline sodium in bulk and tablet dosage form. The results presented are statistically validated in accordance with the guidelines provided by ICH (International conference on harmonization). The solvents used were 0.1N sodium hydroxide and double distilled water. With an absorption maxima of 272 nm, the drug followed a linear relationship in the range of 5-55μg/ml while the correlation coefficient was at 0.9983. The recovery was 99.90% (+0.50) and the coefficient of variance was found to be less than 5%.
In the present study, spectrophotometric method was developed and validated using multicomponent ... more In the present study, spectrophotometric method was developed and validated using multicomponent mode for simultaneous estimation of Salbutamol Sulphate and Theophylline in combined tablet dosage form. Distilled water was used as a solvent. Considering the absorption of both the analytes in the range of 200 nm -320 nm, four wavelength of equal interval of 40 nm were selected such as 320 nm, 280 nm, 240 nm and 200 nm for analysis of these drugs. The mean percentage assay was found as 100.50% and 100.79% for Salbutamol Sulphate and Theophylline respectively. Results of analysis were validated statistically in accordance with ICH guidelines for accuracy and precision of the method. The results of recovery study were observed in the range of 93.26–99.24%. for Salbutamol Sulphate and 97.39–103.615% for Theophylline, While, the % RSD for precision study was observed less than 1%. So, it can be concluded that, the developed method is simple, accurate, economical and easy to apply, making i...
Asian Journal of Pharmaceutical and Clinical Research, 2014
Objective – To develop and validate RP-HPLC method for estimation of Pimozide in bulk and its tab... more Objective – To develop and validate RP-HPLC method for estimation of Pimozide in bulk and its tablet dosage form. Method- Chromatographic separation was carried out on Grace Smart RP-18 column (250 mm X 4.6 mm inner diameter; 5µm particle size) using a mobile phase consisted of Acetonitrile: 50 mM Disodium hydrogen phosphate buffer (pH 6.2, adjusted by 1 % ortho phosphoric acid) in the ratio of 60: 40 %v/v. The flow rate was maintained at 1 ml/min and UV detection was measured at 280 nm. Propyphenazone was used as an internal standard. Results-The calibration curve of Pimozide was linear in range of 5- 100 µg/ml. The mean % assay of marketed formulation was found to be 101.02 % and % recovery was observed in the range of 99.23- 101.91 %. Relative standard deviation for precision study was found less than 2 %. The LOD and LOQ values were found to be 0.553 µg/ml and 1.678 µg/ml respectively. Conclusion- The developed method is simple rapid and easy to apply, making it suitable for rou...
In the present research work, absorbance correction method and first order derivative spectrophot... more In the present research work, absorbance correction method and first order derivative spectrophotometric method have been developed for the simultaneous estimation of Metaxalone and Diclofenac potassium in tablet dosage form. Methanol was used as a solvent in both the methods. The wavelengths of absorption of Metaxalone and Diclofenac potassium were found to be 279 nm and 300 nm respectively and absorbencies at theses wavelengths were used for absorption correction method, while in the first order derivative spectrophotometric method, amplitudes were measured at 302.42nm for Metaxalone (zero cross for Diclofenac potassium) and 282.08 nm for Diclofenac potassium (zero cross for Metaxalone). Metaxalone was found to be linear in the concentration range of 20 to 180µg/ml at 279nm while Diclofenac potassium was found to be linear in the concentration range 5 to 35µg/ml at 300nm and 3 to 24 µg/ml at 279nm. The marketed formulation was analysed using these methods and the results were obta...
A simple, sensitive and specific UV spectrophotometric method has been proposed for the estimatio... more A simple, sensitive and specific UV spectrophotometric method has been proposed for the estimation of Isotretinoin in bulk and soft gelatin capsule for routine analysis. The optimum conditions for analysis were established and validated in conformance with ICH guidelines. It was observed, the absorbance maximum (λmax) for Isotretinoin was 344 nm in methanol and the linearity was in the range of 1-8 μg/ml with coefficient of correlation as 0.9994. The lower limit of detection and the limit of quantification were found to be 0.2519 and 0.7634μg/ml respectively.
In the present study, a reverse phase high performance liquid chromatographic method was develope... more In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Rasagiline in tablet dosage form with the use of Caffeine as an internal standard. Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile: isopropyl alcohol: water (10:20:70, v/v/v) adjusted at pH 2.5 with o-phosphoric acid (1%). The flow rate was maintained at 0.8 ml min−1 and UV detection was measured at 210 nm. The calibration curve was linear over the range 1–200μgml−1. R.S.D. for precision was <3%. The results of accuracy study was observed in the range of 95.16% to 102.24% with R.S.D. < 3%. LOD and LOQ were found to be 0.4044μgml−1 and 1.2133μgml−1, respectively. The method was simple, rapid, and easy to apply, making it suitable for routine analysis of Rasagiline in tablet dosage form.
A Multicomponent Spectrophotometric method for simultaneous estimation of Ketotifen fumarate and ... more A Multicomponent Spectrophotometric method for simultaneous estimation of Ketotifen fumarate and Salbutamol sulphate in combined tablet dosage form has been proposed. Considering the absorption spectra of both analytes in the range of 220-360 nm in water, the four wavelengths of equal intervals of 40 nm were selected as 350 nm, 320 nm, 280 nm and 240 nm to produce calibration curves of mixture of analytes. The present method used inbuilt application of instrument (Shimadzu Pharm Spec 1700, UV-Visible Spectrophotometer) to quantify Ketotifen fumarate and Salbutamol sulphate in formulation. The mean % assay was found as 102.90% and 101.05% for Ketotifen fumarate and Salbutamol sulphate respectively. The method has been validated in accordance with ICH guidelines for accuracy and precision. The mean % recovery was found in range of 98.85 to 99.62% for Ketotifen fumarate and 99.08 to 100.65 % for Salbutamol sulphate, while % RSD was observed as less than 1% for precision study. The deve...
In present research work, a simple Multicomponent Spectrophotometric method for simultaneous esti... more In present research work, a simple Multicomponent Spectrophotometric method for simultaneous estimation of Ketotifen fumarate and Cetirizine dihydrochloride in pharmaceutical dosage was developed. The method used is inbuilt application of instrument (Shimadzu Pharm Spec 1700, UV spectrophotometer) to quantify Ketotifen fumarate and Cetirizine dihydrochloride in formulation. Considering the absorption of both analytes in the range of 200-360 nmin water, the wavelength intervals of 40 nmwas selected such as 360 nm, 320 nm, 280 nm, 240 nm and 200 nm for measurements. The serial dilutions of standard were scanned and absorptions were stored and sample concentration was determined by the instrument. The mean assay was found as about 100.3 %. The method has been validated in accordancewith ICH guidelines for accuracy and precision. Result of recovery study was observed in the range of 98.78% to 101.48% forKetotifen fumarate and 98.77%to 101.48%forCetirizine dihydrochloride. The % RSD for ...
Two methods for simultaneous estimation of Aspirin and Ticlopidine hydrochloride in combination h... more Two methods for simultaneous estimation of Aspirin and Ticlopidine hydrochloride in combination have been developed using 0.1N hydrochloric acid as a solvent. The sample solution was scanned in the range 190-400nm at fast speed. The resulted spectrum was converted into first and second order derivative (using A e=16, scaling factor 100). In second order derivative spectroscopy Aspirin was measured at 303nm while Ticlopidine hydrochloride was determined at 288nm. In second method, amplitudes of first order derivative were measured at 279.2 and 288.2nm and the concentration of Ticlopidine andAspirin were estimated by using simultaneous equation method. The linearity ranges for Aspirin and Ticlopidine hydrochloride were found to be 20-200µg/ml and 100- 700µg/ml respectively at 279.2nm, 288.2nm for first order and 288nm, 303nm for second order derivative method. The accuracy of the methods was assessed by recovery studies was found to be 95.95% and 101.49% for second order derivative me...
Journal of Analytical & Bioanalytical Techniques, 2012
A simple Multicomponent Spectrophotometric method for simultaneous estimation of Metaxalone and D... more A simple Multicomponent Spectrophotometric method for simultaneous estimation of Metaxalone and Diclofenac Potassium in combined tablet dosage form had been proposed. Considering the absorption spectra of both analytes in the range of 220-350 nm in methanol, the four wavelengths of equal intervals of 40 nm were selected as 350 nm, 310 nm, 270 nm and 230 nm to produce calibration curves of mixture of analytes. The present method used inbuilt application of instrument (Shimadzu Pharm Spec 1700, UV spectrophotometer) to quantify Metaxalone and Diclofence potassium in formulation. The method had been validated in accordance with ICH guidelines for accuracy and precision.
In the present study, a reverse phase high performance liquid chromatographic method was develope... more In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Citicoline sodium in pharmaceutical dosage form. Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile: water (20:80, v/v) adjusted at pH 3.0 using 1% orthophosphoric acid. Flow rate was maintained at 0.7ml min and UV detection was carried at 260 nm. Caffeine was used as an internal standard. The calibration curve was found to be linear over the range 1–500μgml. The results of accuracy study were ranged between 99.32% and 101.90% with a R.S.D. less than 1%. LOD and LOQ were 1.4μgml and 4.3μgml, respectively. The method was found to be simple, rapid, and easy to apply, making it very suitable for routine analysis of Citicoline sodium in pharmaceutical dosage form.
In the present study, a reverse phase high performance liquid chromatographic method was develope... more In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Diacerein (DIA) andAceclofenac (ACE) in tablet dosage formwith the use of Paracetamol (PCM) as an internal standard (IS). Chromatographic separation was carried out on a RP-18 column using amobile phase consisting of acetonitrile : water (60:40, v/v) 0f pH 3.0,adjusted with o-phosphoric acid (1%). The flow rate was maintained at 1.0ml/min and UV detection was carried at 268nm. The calibration curve was found linear over the range 10-150µg/ml for DIA and 20-300µg/ml. Relative Standard deviation (RSD) for precision was found to be less than 3%. The results of accuracy study were observed in the range of 99.50%to100.18%withRSD less than 1%. Limit of detection (LOD)was found to be 0.9839µg/ml for DIA and 1.9479µg/ml for ACE. Limit of quantification (LOQ)was found to be 2.9816µg/ml for DIA and 5.9030µg/ ml forACE. The developed analyticalmethod was fou...
A specific, accurate, precise and sensitive validated reverse phase liquid chromatographic (RP-HP... more A specific, accurate, precise and sensitive validated reverse phase liquid chromatographic (RP-HPLC) method has been developed for the simultaneous estimation of Ofloxacin, Ornidazole and its isomer in bulk drug as well as tablet dosage form. Drugs were analysed on C18 column using mobile phase acetonitrile: methanol: 0.025M phosphate buffer (pH 3.0) (30:10:60 v/v/v). A flow rate was maintained at 1.0 ml/min and detection was made at 318 nm. The retention time for Ofloxacin, Ornidazole and its isomer was found to be 4.04, 5.82 and 6.77 min respectively. Proposed method was validated for accuracy, precision, linearity and range, ruggedness. Linearity of Ofloxacin and Ornidazole was in the range of 2–40 μg/ml and 5–100 μg/ml respectively. Average percentage recoveries obtained for Ofloxacin and Ornidazole were 100.20% and 100.9%.
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