International journal of pharma and bio sciences, 2014
Polyhydroxyalkanoates (PHAs) are a group of microbial biodegradable polymers having potential to ... more Polyhydroxyalkanoates (PHAs) are a group of microbial biodegradable polymers having potential to replace synthetic plastics. Several factors are known to influence PHA biosynthesis e.g., bacterial strain used, carbon and nitrogen sources provided, culture conditions, etc. These factors interact with each other during productionand their interaction can be assessed using statistical methods. In the present study, Taguchi Design of Experiments is performed to find out the effect of six different factors viz. incubation period, medium: flask volume ratio, pH of production medium, concentration of maltose, yeast extract and sodium chloride at three levels on production of PHA with 18 well defined experiments (L18 Orthogonal Array). The data obtained was analyzed statistically and it was found that incubation period was the most significant factor contributing 28.84% to PHA production while concentration of maltose was found to be the least significant (1.81%) in the PHA production.
International journal of pharma and bio sciences, 2012
Twelve moderately haloalkaliphilic bacteria were isolated from sediment and water sample collecte... more Twelve moderately haloalkaliphilic bacteria were isolated from sediment and water sample collected from the alkaline Soda Lake of Lonar, India using Lonar Lake water as enrichment medium. All the isolates were identified based on biochemical characterization, 16S rRNA sequencing and phylogenetic analysis. Isolation of haloalkaliphilic Marinobacter excellens, Alkalimonas delamerensis, Roseinatronobacter monicus and Rhodobaca bogoriensis from Lonar Lake appears to be the first report. Three alkalithermophilic actinomycetes isolated from sediment sample of Lonar Lake using glucose yeast-extract peptone medium were identified as Laceyella sacchari belonging to family Thermoactinomycetaceae. All the 12 bacterial isolates along with 66 previously isolated and identified isolates from Lonar Lake (total 78 isolates) and three isolates of alkalithermophilic actinomycetes were screened for their antimicrobial activity. The alkaliphilic bacteria isolated from Lonar Lake exhibited inhibitory effect against clinical isolates of pathogenic bacteria and phytopathogenic fungi which indicates the possibility of using alkaliphilic bacteria as biological control agents.
World Journal of Microbiology & Biotechnology, Aug 11, 2006
Atrazine sensitive leguminous plants were grown in a soil spiked with atrazine and augmented with... more Atrazine sensitive leguminous plants were grown in a soil spiked with atrazine and augmented with an atrazine-degrading bacterium, Arthrobacter sp. strain MCM B-436, to ascertain its degradative efficiency. Germination and survival of plants was correlated with atrazine removal from soil. This experiment was carried out at laboratory as well as field level, showing consistent results. This bioindicator approach serves as
A laboratory-scale activated sludge process was developed to reduce the pollution load of a dye-i... more A laboratory-scale activated sludge process was developed to reduce the pollution load of a dye-industry waste, containing aniline, phenol, methyl violet and rhodamine B as its major components. The waste exerted an organic load of 5576 mg litre(-1) as the chemical oxygen demand (COD), of 896 mg litre(-1) as total organic carbon (TOC), and had a 31.5 mg litre(-1) phenol content. A microbial sludge, capable of growing on the waste, was developed from cattle dung, adapted to the waste and used as a bioinoculum for the process. This resulted in reductions of 60% in COD, 37% in TOC, and 92% in phenol content, and a decrease in optical density of the colour of the waste from an initial 0.915 to 0.360 at 580 nm. Microorganisms isolated from sludge were identified as Pseudomonas alcaligenes and P. mendocina.
International journal of pharma and bio sciences, 2014
Polyhydroxyalkanoates (PHAs) are a group of microbial biodegradable polymers having potential to ... more Polyhydroxyalkanoates (PHAs) are a group of microbial biodegradable polymers having potential to replace synthetic plastics. Several factors are known to influence PHA biosynthesis e.g., bacterial strain used, carbon and nitrogen sources provided, culture conditions, etc. These factors interact with each other during productionand their interaction can be assessed using statistical methods. In the present study, Taguchi Design of Experiments is performed to find out the effect of six different factors viz. incubation period, medium: flask volume ratio, pH of production medium, concentration of maltose, yeast extract and sodium chloride at three levels on production of PHA with 18 well defined experiments (L18 Orthogonal Array). The data obtained was analyzed statistically and it was found that incubation period was the most significant factor contributing 28.84% to PHA production while concentration of maltose was found to be the least significant (1.81%) in the PHA production.
International journal of pharma and bio sciences, 2012
Twelve moderately haloalkaliphilic bacteria were isolated from sediment and water sample collecte... more Twelve moderately haloalkaliphilic bacteria were isolated from sediment and water sample collected from the alkaline Soda Lake of Lonar, India using Lonar Lake water as enrichment medium. All the isolates were identified based on biochemical characterization, 16S rRNA sequencing and phylogenetic analysis. Isolation of haloalkaliphilic Marinobacter excellens, Alkalimonas delamerensis, Roseinatronobacter monicus and Rhodobaca bogoriensis from Lonar Lake appears to be the first report. Three alkalithermophilic actinomycetes isolated from sediment sample of Lonar Lake using glucose yeast-extract peptone medium were identified as Laceyella sacchari belonging to family Thermoactinomycetaceae. All the 12 bacterial isolates along with 66 previously isolated and identified isolates from Lonar Lake (total 78 isolates) and three isolates of alkalithermophilic actinomycetes were screened for their antimicrobial activity. The alkaliphilic bacteria isolated from Lonar Lake exhibited inhibitory effect against clinical isolates of pathogenic bacteria and phytopathogenic fungi which indicates the possibility of using alkaliphilic bacteria as biological control agents.
World Journal of Microbiology & Biotechnology, Aug 11, 2006
Atrazine sensitive leguminous plants were grown in a soil spiked with atrazine and augmented with... more Atrazine sensitive leguminous plants were grown in a soil spiked with atrazine and augmented with an atrazine-degrading bacterium, Arthrobacter sp. strain MCM B-436, to ascertain its degradative efficiency. Germination and survival of plants was correlated with atrazine removal from soil. This experiment was carried out at laboratory as well as field level, showing consistent results. This bioindicator approach serves as
A laboratory-scale activated sludge process was developed to reduce the pollution load of a dye-i... more A laboratory-scale activated sludge process was developed to reduce the pollution load of a dye-industry waste, containing aniline, phenol, methyl violet and rhodamine B as its major components. The waste exerted an organic load of 5576 mg litre(-1) as the chemical oxygen demand (COD), of 896 mg litre(-1) as total organic carbon (TOC), and had a 31.5 mg litre(-1) phenol content. A microbial sludge, capable of growing on the waste, was developed from cattle dung, adapted to the waste and used as a bioinoculum for the process. This resulted in reductions of 60% in COD, 37% in TOC, and 92% in phenol content, and a decrease in optical density of the colour of the waste from an initial 0.915 to 0.360 at 580 nm. Microorganisms isolated from sludge were identified as Pseudomonas alcaligenes and P. mendocina.
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Papers by Pradnya Kanekar