2012 International Conference on Communications, Devices and Intelligent Systems (CODIS), 2012
Abstract Tomato (Solanum lycopersicum L) belongs to the family Solanaceae, which is extensively g... more Abstract Tomato (Solanum lycopersicum L) belongs to the family Solanaceae, which is extensively grown around the world. In Agriculture and Horticulture, the genetic purity of cultivars is critical to farmers, plant breeders, seed producers and as well as regulatory agencies. The genetic and morphological shape based features are used to classify different tomato cultivars and species. However, the large variations present in the shapes of tomato leaves and fruits make it complex enough to classify. In this paper we have applied image ...
An experiment was conducted during 2000 – 05 to evaluate 25 tomato (Lycopersicon esculentum Mill.... more An experiment was conducted during 2000 – 05 to evaluate 25 tomato (Lycopersicon esculentum Mill.) genotypes for resistance to tomato leaf curl virus. The percent disease incidence and coefficient of infection in the genotypes was highest in early autumn (planting in last week of August) followed by spring-summer (planting in first week of February) and autumn-winter (planting in second week of October) seasons suggesting significant influence of environment on symptom expression apart from white fly population. The three moderately resistant lines emerged from the study viz., ‘H-24’, ‘Agata’ and ‘EC-321425’ showing very low coefficient of infection (2-6), were crossed with 5 highly susceptible testers with very high coefficient of infection ( above 21), namely ‘Punjab Chhuhara’, ‘Pusa Ruby’, ‘Ratan’, ‘Hisar Arun’ and ‘Patharkutchi’ in line x tester mating design. The hybrids recorded a range of intermediate disease reaction and it varied widely in 2 seasons suggesting polygenic nat...
Not AvailableThe yellow vein mosaic virus (YVMV) is one of the most serious diseases in okra prod... more Not AvailableThe yellow vein mosaic virus (YVMV) is one of the most serious diseases in okra production, often causing severe losses in commercial fields. Identifying and deploying resistant genotypes and understanding the inheritance of YVMV disease resistance are essential for the okra geneticists to develop an effective breeding strategy. Genetic control of the host resistance to YVMV disease of okra was studied employing six generations (P1, P2, F1, F2, BC1, BC2) of three selected crosses: Tolerant × Tolerant (T × T), Tolerant × Susceptible (T × S) and Susceptible × Susceptible (S × S) among two tolerant and susceptible genotypes. Relationship between disease reaction and different biochemical parameters of the parents and hybrids at three phenological stages (Pre-flowering, flowering and post-flowering) was studied. The inheritance study amply indicated that tolerance to YVMV disease was conditioned by two duplicate dominant genes in Tolerant × Tolerant cross, and by two complementary dominant genes in Tolerant × Susceptible cross. The significant scaling tests and joint scaling test also indicated the presence of digenic epistasis for both the disease reaction characters. The study also suggested that tolerant genotypes appeared in the progeny of even Tolerant × Susceptible cross. Some of the enzyme activities (peroxidase and polyphenol oxidase) and proximate compositions (total phenol and ascorbic acid) in okra leaf exhibited consistent and significant negative correlation with PDI of YVMV disease even over the growth stages suggesting their implication as selection indices for identification of genotype tolerant to YVMV disease. The results suggested modified bulk method of breeding through deferred selection after attaining homozygosity for maximum heterozygous loci.Not Availabl
Different fruit characters including quality characters of tomato (Solanum lycopersicum L.) are i... more Different fruit characters including quality characters of tomato (Solanum lycopersicum L.) are influenced by environment and other factors hence, the present investigation was carried out employing nine genotypes to determine the fruit sampling technique through the study on the variation in different fruit characters in the genotypes, in different clusters of the main stem and different fruits of the same cluster in the same genotype. Considering the variation in fruit characters with the year, genotypes, cluster position and fruit position in the cluster it was suggested to repeat the evaluation of the genotypes and to sample the first and last fruit of the third cluster for recording fruit weight, polar and equatorial diameter and pericarp thickness and to use the composite sample from these fruits for estimation of proximate compositions of fruits, viz. TSS, β carotene and lycopene contents following standard methods to ascertain the genotypic worth for different quantitative a...
Okra production in eastern India at present is severely threatened by whitefly-mediated okra enat... more Okra production in eastern India at present is severely threatened by whitefly-mediated okra enation leaf curl disease (OELCuD). Identification of resistant genotype and understanding the genetic control and biochemical relationship of OELCuD resistance are prerequisite for developing an effective breeding strategy. This study was conducted employing six populations (P 1 , P 2 , F 1 , F 2 , BC 1 and BC 2 ) of two selected (resistant × susceptible (R×S)) crosses. Associationship between severity of OELCuD and biochemical parameters of parents and hybrids at preflowering and flowering stages was studied. Segregation pattern of the genotypes in F 2 generation showing OELCuD reaction of two crosses suggested that two duplicate recessive genes was operative for resistance to OELCuD. Generation mean analysis revealed involvement of both additive and nonadditive effects in the inheritance of disease resistance. Hence, postponement of selection in later generations or intermating among the ...
Indian Journal of Genetics and Plant Breeding, 2001
Genetical studies on fruit weight, equatorial diameter and polar diameter of fruit, fruit shape i... more Genetical studies on fruit weight, equatorial diameter and polar diameter of fruit, fruit shape index, locules/fruit, TSS content of fruit juice, length of style, ovary diameter and pollen diameter were made in the F1 and F2 populations of an interspecific cross L. pimpinellifolium x L. esculentum of tomato. The parents represented a wide range of variation for all the characters. Characteristics of F1 and F2 populations varied in different characters. However, in all the characters genes with negative effect exhibited dominance in both F1 and F2 populations. Group of genes detected for fruit weight were 22–29, for equatorial diameter 11–13, for polar diameter 8–10, for style length 5–7, for pollen diameter 5–8 and 2 groups of genes for locules/fruit and TSS of fruit juice. Only one group of gene was detected for fruit shape index and ovary diameter. Fruits borne by few F2 segregates were medium sized, round to slight flatlish, shiny red and having appreciable TSS content in the fru...
Twenty two genotypes of okra were evaluated for 13 fruit yield and other related characters to de... more Twenty two genotypes of okra were evaluated for 13 fruit yield and other related characters to determine their genetic divergence. Following D2 analysis, the genotypes were grouped into 5 clusters with the highest of 16 genotypes in cluster I. Most of the genotypes were not much divergent based on character constellation but were highly variable for individual character. A genotype apprehended to be under the species other than A. esculentus showed the widest divergence and belonged to the separate cluster. On the basis of high yield, important yield components and fruit quality, 4 diverse and desirable genotypes (MDO-10, LORM-1, KS-410 and MDO-6) were selected. It is proposed that these genotypes may be involved in a multiple crossing programme to recover transgressive segregates with high genetic yield potential.
Association of 12 fruit characters viz., fruit weight, polar diameter of fruit, equatorial diamet... more Association of 12 fruit characters viz., fruit weight, polar diameter of fruit, equatorial diameter of fruit, locule number per fruit, pericarp thickness, TSS, lycopene, â carotene, total sugar, reducing sugar, titrable acidity and ascorbic acid contents of the fruit have been studied utilizing average data over two years on 12 widely divergent genotypes of tomato to determine important selection indices for enhancing fruit qualitycharactersin tomato. Correlation and path coefficient analyses confirmed that medium sized fruits, fewer locules, thick pericarp, high total soluble solids and medium titrable acidity level in the fruits should be considered as the most important selection indices for enhancing lycopene, â carotene and ascorbic acid contents in the fruit.
The investigation was carried out during 2001-07 to examine the magnitude of heterosis in relatio... more The investigation was carried out during 2001-07 to examine the magnitude of heterosis in relation to genetic divergence among 9 parents in a 9 x 9 half-diallel cross of eggplant or brinjal (Solanum melongena L.). The 9 parents were grouped in 6 different clusters in the lot of 70 entries (10 elite varieties, 16 stable breeding lines and 44 indigenous cultivars of India and Bangladesh) based on multivariate analysis using Mahalanobis' D 2 -statistic employing 18 growth, yield components, fruit yield and fruit quality traits from three years evaluation. Diversity of these 9 parental lines was again determined separately based on 4 important characters including fruit yield. The relationship between intra- and inter-cluster divergence, total divergence of the parents and both relative heterosis and heterobeltiosis of 36 crosses for 4 important characters, viz plant height, fruits/plant, fruit weight, and fruit yield/plant, was determined using correlations and linear regression. I...
Indian Journal of Genetics and Plant Breeding, 2008
The present investigation reports four years of study on the expressivity of functional male ster... more The present investigation reports four years of study on the expressivity of functional male sterility gene in the original mutant line UGS-1MS and in different genetic backgrounds. In polyhouse condition, anthers of most of the plants of UGS-1MS (88%) did not dehisce. In the open field conditions under two autumn-winter and one spring-summer seasons over two years only 63.5% plants on an average exhibited non-dehiscent anther character. However, percentage of open fruit set due to natural selfing was significantly lower in the plants showing either complete or partial anther dehisdence because anther dehiscence in these plants have occurred either very late or in few anthers only. Environmental variation for the trait indicates the role of environmentally sensitive modifier genes. Male fertility in all the six F1 hybrids due either to complete or partial anther dehiscence suggested that functional sterility due to anther non-dehiscence was under the control of recessive genes. Wide...
Indian Journal of Genetics and Plant Breeding (The), 2013
ABSTRACT Induction of mutation by gamma rays, ethyl methane sulfonate (EMS) and their combined tr... more ABSTRACT Induction of mutation by gamma rays, ethyl methane sulfonate (EMS) and their combined treatments was studied in three widely divergent genotypes of tomato, EC620176, EC620177 and Patharkutchi. A steady reduction in germination percentage, seedling height and pollen fertility occurred in M 1 generation with the increasing doses / concentrations of mutagens. Combination of gamma radiation and EMS caused more damage followed by EMS treatment and gamma radiation alone in M 1 generation. The LD 50 dose for EC620176, EC620177 and Patharkutchi corresponded to 67.3 Gy, 290.9 Gy and 303.8 Gy gamma radiation, and 0.10%, 0.17 % and 0.38 % EMS treatment, respectively. Highest mutation frequency was resulted by gamma radiation followed by the combined mutagens and EMS treatment. Genotype and mutagen, both, influenced the production of mutants. Mutagenic efficiency of lower doses/concentrations was more compared to higher doses in producing desirable mutants. Mutagenic effectiveness of gamma radiation was the highest followed by sole EMS and combined mutagens. Gamma irradiation (50-150 Gy) was most efficient followed by 0.05-0.10 % EMS and their combination treatment in inducing wide array of macro-mutation in tomato. Five putative mutants with exserted stigma flower, dark green fruit, dwarf plant having pyriform fruit from Patharkutchi, multiparous cyme from EC620177 and chlorophyll deficient mutant from EC620176 that could be isolated in M 2 generation hold promise for their utilization in tomato breeding programme.
2012 International Conference on Communications, Devices and Intelligent Systems (CODIS), 2012
Abstract Tomato (Solanum lycopersicum L) belongs to the family Solanaceae, which is extensively g... more Abstract Tomato (Solanum lycopersicum L) belongs to the family Solanaceae, which is extensively grown around the world. In Agriculture and Horticulture, the genetic purity of cultivars is critical to farmers, plant breeders, seed producers and as well as regulatory agencies. The genetic and morphological shape based features are used to classify different tomato cultivars and species. However, the large variations present in the shapes of tomato leaves and fruits make it complex enough to classify. In this paper we have applied image ...
An experiment was conducted during 2000 – 05 to evaluate 25 tomato (Lycopersicon esculentum Mill.... more An experiment was conducted during 2000 – 05 to evaluate 25 tomato (Lycopersicon esculentum Mill.) genotypes for resistance to tomato leaf curl virus. The percent disease incidence and coefficient of infection in the genotypes was highest in early autumn (planting in last week of August) followed by spring-summer (planting in first week of February) and autumn-winter (planting in second week of October) seasons suggesting significant influence of environment on symptom expression apart from white fly population. The three moderately resistant lines emerged from the study viz., ‘H-24’, ‘Agata’ and ‘EC-321425’ showing very low coefficient of infection (2-6), were crossed with 5 highly susceptible testers with very high coefficient of infection ( above 21), namely ‘Punjab Chhuhara’, ‘Pusa Ruby’, ‘Ratan’, ‘Hisar Arun’ and ‘Patharkutchi’ in line x tester mating design. The hybrids recorded a range of intermediate disease reaction and it varied widely in 2 seasons suggesting polygenic nat...
Not AvailableThe yellow vein mosaic virus (YVMV) is one of the most serious diseases in okra prod... more Not AvailableThe yellow vein mosaic virus (YVMV) is one of the most serious diseases in okra production, often causing severe losses in commercial fields. Identifying and deploying resistant genotypes and understanding the inheritance of YVMV disease resistance are essential for the okra geneticists to develop an effective breeding strategy. Genetic control of the host resistance to YVMV disease of okra was studied employing six generations (P1, P2, F1, F2, BC1, BC2) of three selected crosses: Tolerant × Tolerant (T × T), Tolerant × Susceptible (T × S) and Susceptible × Susceptible (S × S) among two tolerant and susceptible genotypes. Relationship between disease reaction and different biochemical parameters of the parents and hybrids at three phenological stages (Pre-flowering, flowering and post-flowering) was studied. The inheritance study amply indicated that tolerance to YVMV disease was conditioned by two duplicate dominant genes in Tolerant × Tolerant cross, and by two complementary dominant genes in Tolerant × Susceptible cross. The significant scaling tests and joint scaling test also indicated the presence of digenic epistasis for both the disease reaction characters. The study also suggested that tolerant genotypes appeared in the progeny of even Tolerant × Susceptible cross. Some of the enzyme activities (peroxidase and polyphenol oxidase) and proximate compositions (total phenol and ascorbic acid) in okra leaf exhibited consistent and significant negative correlation with PDI of YVMV disease even over the growth stages suggesting their implication as selection indices for identification of genotype tolerant to YVMV disease. The results suggested modified bulk method of breeding through deferred selection after attaining homozygosity for maximum heterozygous loci.Not Availabl
Different fruit characters including quality characters of tomato (Solanum lycopersicum L.) are i... more Different fruit characters including quality characters of tomato (Solanum lycopersicum L.) are influenced by environment and other factors hence, the present investigation was carried out employing nine genotypes to determine the fruit sampling technique through the study on the variation in different fruit characters in the genotypes, in different clusters of the main stem and different fruits of the same cluster in the same genotype. Considering the variation in fruit characters with the year, genotypes, cluster position and fruit position in the cluster it was suggested to repeat the evaluation of the genotypes and to sample the first and last fruit of the third cluster for recording fruit weight, polar and equatorial diameter and pericarp thickness and to use the composite sample from these fruits for estimation of proximate compositions of fruits, viz. TSS, β carotene and lycopene contents following standard methods to ascertain the genotypic worth for different quantitative a...
Okra production in eastern India at present is severely threatened by whitefly-mediated okra enat... more Okra production in eastern India at present is severely threatened by whitefly-mediated okra enation leaf curl disease (OELCuD). Identification of resistant genotype and understanding the genetic control and biochemical relationship of OELCuD resistance are prerequisite for developing an effective breeding strategy. This study was conducted employing six populations (P 1 , P 2 , F 1 , F 2 , BC 1 and BC 2 ) of two selected (resistant × susceptible (R×S)) crosses. Associationship between severity of OELCuD and biochemical parameters of parents and hybrids at preflowering and flowering stages was studied. Segregation pattern of the genotypes in F 2 generation showing OELCuD reaction of two crosses suggested that two duplicate recessive genes was operative for resistance to OELCuD. Generation mean analysis revealed involvement of both additive and nonadditive effects in the inheritance of disease resistance. Hence, postponement of selection in later generations or intermating among the ...
Indian Journal of Genetics and Plant Breeding, 2001
Genetical studies on fruit weight, equatorial diameter and polar diameter of fruit, fruit shape i... more Genetical studies on fruit weight, equatorial diameter and polar diameter of fruit, fruit shape index, locules/fruit, TSS content of fruit juice, length of style, ovary diameter and pollen diameter were made in the F1 and F2 populations of an interspecific cross L. pimpinellifolium x L. esculentum of tomato. The parents represented a wide range of variation for all the characters. Characteristics of F1 and F2 populations varied in different characters. However, in all the characters genes with negative effect exhibited dominance in both F1 and F2 populations. Group of genes detected for fruit weight were 22–29, for equatorial diameter 11–13, for polar diameter 8–10, for style length 5–7, for pollen diameter 5–8 and 2 groups of genes for locules/fruit and TSS of fruit juice. Only one group of gene was detected for fruit shape index and ovary diameter. Fruits borne by few F2 segregates were medium sized, round to slight flatlish, shiny red and having appreciable TSS content in the fru...
Twenty two genotypes of okra were evaluated for 13 fruit yield and other related characters to de... more Twenty two genotypes of okra were evaluated for 13 fruit yield and other related characters to determine their genetic divergence. Following D2 analysis, the genotypes were grouped into 5 clusters with the highest of 16 genotypes in cluster I. Most of the genotypes were not much divergent based on character constellation but were highly variable for individual character. A genotype apprehended to be under the species other than A. esculentus showed the widest divergence and belonged to the separate cluster. On the basis of high yield, important yield components and fruit quality, 4 diverse and desirable genotypes (MDO-10, LORM-1, KS-410 and MDO-6) were selected. It is proposed that these genotypes may be involved in a multiple crossing programme to recover transgressive segregates with high genetic yield potential.
Association of 12 fruit characters viz., fruit weight, polar diameter of fruit, equatorial diamet... more Association of 12 fruit characters viz., fruit weight, polar diameter of fruit, equatorial diameter of fruit, locule number per fruit, pericarp thickness, TSS, lycopene, â carotene, total sugar, reducing sugar, titrable acidity and ascorbic acid contents of the fruit have been studied utilizing average data over two years on 12 widely divergent genotypes of tomato to determine important selection indices for enhancing fruit qualitycharactersin tomato. Correlation and path coefficient analyses confirmed that medium sized fruits, fewer locules, thick pericarp, high total soluble solids and medium titrable acidity level in the fruits should be considered as the most important selection indices for enhancing lycopene, â carotene and ascorbic acid contents in the fruit.
The investigation was carried out during 2001-07 to examine the magnitude of heterosis in relatio... more The investigation was carried out during 2001-07 to examine the magnitude of heterosis in relation to genetic divergence among 9 parents in a 9 x 9 half-diallel cross of eggplant or brinjal (Solanum melongena L.). The 9 parents were grouped in 6 different clusters in the lot of 70 entries (10 elite varieties, 16 stable breeding lines and 44 indigenous cultivars of India and Bangladesh) based on multivariate analysis using Mahalanobis' D 2 -statistic employing 18 growth, yield components, fruit yield and fruit quality traits from three years evaluation. Diversity of these 9 parental lines was again determined separately based on 4 important characters including fruit yield. The relationship between intra- and inter-cluster divergence, total divergence of the parents and both relative heterosis and heterobeltiosis of 36 crosses for 4 important characters, viz plant height, fruits/plant, fruit weight, and fruit yield/plant, was determined using correlations and linear regression. I...
Indian Journal of Genetics and Plant Breeding, 2008
The present investigation reports four years of study on the expressivity of functional male ster... more The present investigation reports four years of study on the expressivity of functional male sterility gene in the original mutant line UGS-1MS and in different genetic backgrounds. In polyhouse condition, anthers of most of the plants of UGS-1MS (88%) did not dehisce. In the open field conditions under two autumn-winter and one spring-summer seasons over two years only 63.5% plants on an average exhibited non-dehiscent anther character. However, percentage of open fruit set due to natural selfing was significantly lower in the plants showing either complete or partial anther dehisdence because anther dehiscence in these plants have occurred either very late or in few anthers only. Environmental variation for the trait indicates the role of environmentally sensitive modifier genes. Male fertility in all the six F1 hybrids due either to complete or partial anther dehiscence suggested that functional sterility due to anther non-dehiscence was under the control of recessive genes. Wide...
Indian Journal of Genetics and Plant Breeding (The), 2013
ABSTRACT Induction of mutation by gamma rays, ethyl methane sulfonate (EMS) and their combined tr... more ABSTRACT Induction of mutation by gamma rays, ethyl methane sulfonate (EMS) and their combined treatments was studied in three widely divergent genotypes of tomato, EC620176, EC620177 and Patharkutchi. A steady reduction in germination percentage, seedling height and pollen fertility occurred in M 1 generation with the increasing doses / concentrations of mutagens. Combination of gamma radiation and EMS caused more damage followed by EMS treatment and gamma radiation alone in M 1 generation. The LD 50 dose for EC620176, EC620177 and Patharkutchi corresponded to 67.3 Gy, 290.9 Gy and 303.8 Gy gamma radiation, and 0.10%, 0.17 % and 0.38 % EMS treatment, respectively. Highest mutation frequency was resulted by gamma radiation followed by the combined mutagens and EMS treatment. Genotype and mutagen, both, influenced the production of mutants. Mutagenic efficiency of lower doses/concentrations was more compared to higher doses in producing desirable mutants. Mutagenic effectiveness of gamma radiation was the highest followed by sole EMS and combined mutagens. Gamma irradiation (50-150 Gy) was most efficient followed by 0.05-0.10 % EMS and their combination treatment in inducing wide array of macro-mutation in tomato. Five putative mutants with exserted stigma flower, dark green fruit, dwarf plant having pyriform fruit from Patharkutchi, multiparous cyme from EC620177 and chlorophyll deficient mutant from EC620176 that could be isolated in M 2 generation hold promise for their utilization in tomato breeding programme.
Uploads
Papers by Pranab Hazra