An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C... more An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C) were developed and validated for the determination of Clobazam in bulk and tablet dosage forms. In Method-A, a PEAK chromatographic system equipped with Zodiac C-18 (250mm, 4.6mm and 5μm) column, mobile phase of composition 10:30:60 (v/v/v) of tetra hydro furan (THF), methanol (MeOH) and acetonitrile (ACN), UV detector were adopted for the chromatographic analysis. The output signal was monitored at a detection wavelength at 227 nm and integrated by PEAK Chromatographic Software version 1.06. In spectrophotometric methods, the chromogenic reagents such as Fe (II)-1, 10 phenanthroline (Methods-B) and ferricyanide-Fe (III) (Method-C) reagents were used for color development for the estimation of Clobazam in pure and formulations. The developed methods were validated according ICH guidelines and adopted for the assay of clobazam in the bulk drug and formulations.
Asian Journal of Pharmaceutical and Clinical Research, 2015
Objective: The main objective of the present investigation was to develop a simple isocratic stab... more Objective: The main objective of the present investigation was to develop a simple isocratic stability indicating reversed phase liquid chromatographic method and validate the proposed method and to apply it for the estimation of ketorolac tromethamine, a non-steroidal anti-inflammatory drug in pure and pharmaceutical formulations. Methods: Waters high performance liquid chromatographic 2695 series system, inertsil ODS, C18 (150 mm × 4.6 mm × 5.0 μ) column and UV-visible detector with photo diode array detection was adopted for the method development. The components were separated by injecting about 20 μL working standard solution of concentration 5 μg/mL, using mobile phase prepared by mixing buffer of 100 mM potassium dihydrogen orthophosphate solution of pH 4.5 and acetonitrile in the ratio 60:40 v/v at a flow rate of 0.8 mL/minute, and the components were detected at a wavelength of 316 nm. Results: The proposed method was validated as per ICH guidelines. The developed method wa...
An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C... more An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C) were developed and validated for the determination of Clobazam in bulk and tablet dosage forms. In Method-A, a PEAK chromatographic system equipped with Zodiac C-18 (250mm, 4.6mm and 5μm) column, mobile phase of composition 10:30:60 (v/v/v) of tetra hydro furan (THF), methanol (MeOH) and acetonitrile (ACN), UV detector were adopted for the chromatographic analysis. The output signal was monitored at a detection wavelength at 227 nm and integrated by PEAK Chromatographic Software version 1.06. In spectrophotometric methods, the chromogenic reagents such as Fe (II)-1, 10 phenanthroline (Methods-B) and ferricyanide-Fe (III) (Method-C) reagents were used for color development for the estimation of Clobazam in pure and formulations. The developed methods were validated according ICH guidelines and adopted for the assay of clobazam in the bulk drug and formulations.
Asian Journal of Pharmaceutical and Clinical Research, 2015
Objective: The main objective of the present investigation was to develop a simple isocratic stab... more Objective: The main objective of the present investigation was to develop a simple isocratic stability indicating reversed phase liquid chromatographic method and validate the proposed method and to apply it for the estimation of ketorolac tromethamine, a non-steroidal anti-inflammatory drug in pure and pharmaceutical formulations. Methods: Waters high performance liquid chromatographic 2695 series system, inertsil ODS, C18 (150 mm × 4.6 mm × 5.0 μ) column and UV-visible detector with photo diode array detection was adopted for the method development. The components were separated by injecting about 20 μL working standard solution of concentration 5 μg/mL, using mobile phase prepared by mixing buffer of 100 mM potassium dihydrogen orthophosphate solution of pH 4.5 and acetonitrile in the ratio 60:40 v/v at a flow rate of 0.8 mL/minute, and the components were detected at a wavelength of 316 nm. Results: The proposed method was validated as per ICH guidelines. The developed method wa...
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Papers by Prof.GOLKONDA RAMU