Background Myocardial Infarction (MI), also known as heart attack, is one of the most common card... more Background Myocardial Infarction (MI), also known as heart attack, is one of the most common cardiovascular diseases. Although certain drugs or mechanical means are used, day by day natural products such as herbs and spices based MI treatment is getting much popularity over the drugs or mechanical means for their pharmacological effects and have low or no side effects. This study was designed to assess the cardio-protective effect of methanolic extract of Bangladeshi multi clove garlic (Allium sativum) cultivar, a highly believed spice having cardioprotective activity, against isoproterenol (ISO) induced MI through cardiac histopathology as well as cardiac apoptotic caspase-3 gene expression study in female Wistar albino rats. Four groups containing 35 rats treated with respective agents like distill water / garlic extract (200 mg/kg-body-weight/day) up to 28 days and normal saline / ISO (100 mg/kg-body-weight/day) on 29th and 30th day were sacrificed (two rats/group/sacrifice) on t...
Context: It is necessary to focus on the importance of adopting micropropagation technique for ma... more Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials and Methods: Shoot tips and nodal segments were used for multiple shoot induction. All explants were cultured on MS medium supplemented with various plant growth regulators. HgCl2 was used as surface sterilizing agent. For in vitro rooting, individual shoots (3-4 cm) were cut from the proliferated shoot cultures and implanted on half and full strength of MS with different concentrations and combinations of NAA and IAA. The cultures were incubated for 16 h photoperiod at 25 ± 2ºC under a fluorescent light. Visual observation of culture was made every week. Data on shoot induction and proliferatio...
Dhaka University Journal of Pharmaceutical Sciences, 2015
not available DOI: http://dx.doi.org/10.3329/dujps.v13i2.21904 Dhaka Univ. J. Pharm. Sci. 13(2): ... more not available DOI: http://dx.doi.org/10.3329/dujps.v13i2.21904 Dhaka Univ. J. Pharm. Sci. 13(2): 221-224, 2014 (December)
... In the recent years, the development of resistance of pathogens against antibiotics has becom... more ... In the recent years, the development of resistance of pathogens against antibiotics has become a difficult issue caused by the indiscriminate use of modern antibiotics (WHO, 1983; Kunin, 1993; Okemo et al., 2003; Arya et al., 2010; Dash et al., 2011). ...
ABSTRACT In this study, 42 years of tidal records and landfall TC best tracks in Japan were used ... more ABSTRACT In this study, 42 years of tidal records and landfall TC best tracks in Japan were used to demonstrate that TC pre-landfall forward speed is significantly correlated with maximum storm surge height. Coastal morphology was the determining factor for the correlation between storm surge and TC forward speed. Fast-moving TCs tended to amplify the storm surge along open coastlines (Pearson correlation coefficient, R = 0.62), but reduce it in semi-enclosed bays (R = −0.52). The negative correlation contrasts with the general perception that the coincidence of TC wind speed and forward speed vectors generates a larger storm surge. The influence of coastal morphology was most prominent for TCs with a central pressure lower than 956 hPa. Tropical cyclone (TC) operational forecasts are continuously improving; however, there is still scope to improve the precision of storm surge predictions. These findings could contribute to the improvement of storm surge forecasting and provide emergency management personnel with more precise early warnings of dangerous storm surges.
Environmental monitoring and assessment, Jan 8, 2018
In this research, heavy metal accumulation pattern was investigated using the data measured from ... more In this research, heavy metal accumulation pattern was investigated using the data measured from the soil, paddy plants, and irrigation water samples in Jessore district in Bangladesh with the aid of principal component analysis. A total of 28 samples representing farmland soil and irrigation water along with paddy plant were collected from 28 locations in the Jessore district in November 2016. In agricultural soil, arsenic (As) and nickel (Ni) were found 2.78 and 1.11 times more concentrated than their background values. In addition, 89% of the sample sites exhibited enhanced As concentrations relative to the background value. Principal component analysis (PCA) of soil data showed strong homogeneity in many species (e.g., Ni, Cu, Fe, and As) to reflect intense agricultural activities. In contrast, Pb showed no such homogeneity in soil accumulation pattern. In plant samples, Cu, Fe, and As were strongly correlated and homologous. This homology of pollution was in agreement with the ...
Journal of microbiology, biotechnology and food sciences, 2015
Lablab purpureus (L.) Sweet is one of the most important and nutritious vegetables as well as pul... more Lablab purpureus (L.) Sweet is one of the most important and nutritious vegetables as well as pulse in Bangladesh and is grown extensively all over the country. It suffers from various fungal infections, which reduce greatly its quality and quantity. Seed-borne and seed associated fungi are one of the factors of substantial damages of the bean genotypes. Seed- borne and seed associated fungi of eleven genotypes of L. purpureus (L.) Sweet and their various controlling methods were studied. From eleven bean seed samples, seven types of fungal pathogens were isolated and identified. The most predominant fungi species were Aspergillus spp. followed by Fusarium sp. and Rhizopus sp. Comparatively less frequent fungi were Penicillium sp., Curvularia sp., Colletotrichum sp. and Alternaria sp. Plant extracts (Lawsonia inermis, Azadirachta indica and Allium sativum), cow urine, hot water and chemical fungicides (Bavistin, redomil and dithane M-45) were used to observe the efficacy of them aga...
The aim of this study is to investigate the hypoglycemic and hypolipidemic effects of Coccinia co... more The aim of this study is to investigate the hypoglycemic and hypolipidemic effects of Coccinia cordifolia leaf and root powder on alloxan induced type 2 diabetic Long-Evans rats. Oral feeding of the C. cordifolia leaf and root powder slightly decreased serum total cholesterol, triglyceride levels and LDL-cholesterol as compared with leaf, root and standard drug. C. cordifolia leaf showed more significant (p < 0.05, 0.01 and 0.001) effect on blood glucose level when compared with root and standard drug (glibenclamide, 5 mg/kg). At the mean time, rats’ serum insulin level markedly increased; leaf does showed more significant (p < 0.05, 0.01 and 0.001) effect than glibenclamide control group and root powder feeding group. But C. cordifolia did not show any significant effect on HDL-cholesterol and liver glycogen after 21st day feeding. Thus, the results of the experimental study suggest that C. cordifolia possesses hypoglycemic and hypolipidemic effects and is able to ameliorate ...
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: Bacillus sp. is now broadly responsible for various human infection and food spoilage.... more Objective: Bacillus sp. is now broadly responsible for various human infection and food spoilage. At present, identification and molecular characterization of major types of Bacillus sp. are important for human being. Methods: Antibacterial activities of six different concentrations each of ethanol, ethyl acetate and chloroform extracts of Coccinia grandis (L.) leaf on soil bacteria were assessed on the basis of disk diffusion method and the zone of inhibition was measured in mm unit. Different Bacillus sp. was characterized by using 16S rDNA sequencing. Results: Six different types of Bacillus sp. were identified depending on 16S rDNA sequencing and NCBI database information. It has also been shown that the best extract was ethyl acetate due to its highest zone of inhibition (Paenibacillus sp. BF38 (23.0±0.41), Terribacillus sp. 3LF (21.0±0.00), Bacillus simplex (23.0±0.43), Bacillus cereus (23.5±0.70), Paenibacillus sp.L32 (22.5±0.37), Bacillus megaterium (23.0±0.04)) as compared to other two extracts. Conclusion: The efficacy of Coccinia grandis (L.) leaf extracts as natural antibacterial agent was found ethyl acetate extract > ethanol extract > chloroform extract.
Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged m... more Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged mycelial culture of Lentinus lepideus were studied and their effectiveness was compared with lipopolysaccharide (LPS). The influence of the LL-EX on macrophage cellular lysosomal enzyme activity was to stimulate up to 267%, 392%, and 464% at the level of 10, 50, and 100 microg/ml, respectively. When the LL-EX was further fractionated into LL-Fr.I and Fr.II by Sepharose CL-6B gel chromatography, the cellular lysosomal enzyme activity of LL-Fr.II (2.1- fold) was higher than Fr.I (1.2-fold). Moreover, both LL-Fr.I and Fr.II stimulated the cytokines IL-1beta, TNF-alpha, and IL-6 in macrophages. In mixed lymphocyte reaction, LL-Fr.I and Fr.II enhanced the splenocyte proliferation up to 1.2-fold and 1.4-fold (50 microg/ml), respectively, stimulating only T lymphocytes. The fractions of LL-EX not show any direct toxicity against human gastric adenocarcinoma cell (AGS). The molecular masses of LL...
Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. ... more Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate belonged to Streptomyces albidoflavus. Preliminary screening revealed that the isolate was active against fungi and bacteria. Self-directing optimization was employed to determine the best combination of parameters such as carbon and nitrogen source, pH and temperature. Nutritional and culture conditions for the production of antibiotics by this organism under shake-flask conditions were also optimized. Maltose (5%) and soytone (5%) were found to be the best carbon and nitrogen sources for the production of antibiotics by S. albidoflavus C247. Additionally, 62.89% mycelial growth inhibition was achieved when the organism was cultured at 30℃ and pH 6.5. Ethyl acetate (EtOAc) was the best extraction solvent for the isolation of the antibiotics, and 100 µg/ml of EtOAc extract was found to inhi...
Asian Pacific Journal of Tropical Biomedicine, 2012
ABSTRACT Genistein recognized as phytoestrogens is one of the most extensively studied isoflavone... more ABSTRACT Genistein recognized as phytoestrogens is one of the most extensively studied isoflavones. It comprises of significant portion of Asian diet including Japanese and Chinese cuisine in the form of Soy food products. Evidence showed that geinstein increases osteoblasts formation as well as decreases osteoclast production. It plays an important role in immunity; such as suppression of delayed hypersensitivity and increases host resistance to B16F10 tumor by proliferating cytotoxic T and NK cells. It also decreases the activity of lipoprotein lipase which in turn inhibits lipogenesis and prevents the uptake of glucose in type 2 diabetic in rats. Geinstein play important role in reproductive system where it regulates the productive of oestrogen and progesterone. Moreover Geinstein has the ability to inhibit the tumor and cancer cell proliferation. Numerous beneficial effect of Geinstein including cancer treatment and function in immunity, obesity, diabetes and reproductivity Geinstein proves the potentiality of phytoestrogens as a source of bioactive substance.
Background Myocardial Infarction (MI), also known as heart attack, is one of the most common card... more Background Myocardial Infarction (MI), also known as heart attack, is one of the most common cardiovascular diseases. Although certain drugs or mechanical means are used, day by day natural products such as herbs and spices based MI treatment is getting much popularity over the drugs or mechanical means for their pharmacological effects and have low or no side effects. This study was designed to assess the cardio-protective effect of methanolic extract of Bangladeshi multi clove garlic (Allium sativum) cultivar, a highly believed spice having cardioprotective activity, against isoproterenol (ISO) induced MI through cardiac histopathology as well as cardiac apoptotic caspase-3 gene expression study in female Wistar albino rats. Four groups containing 35 rats treated with respective agents like distill water / garlic extract (200 mg/kg-body-weight/day) up to 28 days and normal saline / ISO (100 mg/kg-body-weight/day) on 29th and 30th day were sacrificed (two rats/group/sacrifice) on t...
Context: It is necessary to focus on the importance of adopting micropropagation technique for ma... more Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials and Methods: Shoot tips and nodal segments were used for multiple shoot induction. All explants were cultured on MS medium supplemented with various plant growth regulators. HgCl2 was used as surface sterilizing agent. For in vitro rooting, individual shoots (3-4 cm) were cut from the proliferated shoot cultures and implanted on half and full strength of MS with different concentrations and combinations of NAA and IAA. The cultures were incubated for 16 h photoperiod at 25 ± 2ºC under a fluorescent light. Visual observation of culture was made every week. Data on shoot induction and proliferatio...
Dhaka University Journal of Pharmaceutical Sciences, 2015
not available DOI: http://dx.doi.org/10.3329/dujps.v13i2.21904 Dhaka Univ. J. Pharm. Sci. 13(2): ... more not available DOI: http://dx.doi.org/10.3329/dujps.v13i2.21904 Dhaka Univ. J. Pharm. Sci. 13(2): 221-224, 2014 (December)
... In the recent years, the development of resistance of pathogens against antibiotics has becom... more ... In the recent years, the development of resistance of pathogens against antibiotics has become a difficult issue caused by the indiscriminate use of modern antibiotics (WHO, 1983; Kunin, 1993; Okemo et al., 2003; Arya et al., 2010; Dash et al., 2011). ...
ABSTRACT In this study, 42 years of tidal records and landfall TC best tracks in Japan were used ... more ABSTRACT In this study, 42 years of tidal records and landfall TC best tracks in Japan were used to demonstrate that TC pre-landfall forward speed is significantly correlated with maximum storm surge height. Coastal morphology was the determining factor for the correlation between storm surge and TC forward speed. Fast-moving TCs tended to amplify the storm surge along open coastlines (Pearson correlation coefficient, R = 0.62), but reduce it in semi-enclosed bays (R = −0.52). The negative correlation contrasts with the general perception that the coincidence of TC wind speed and forward speed vectors generates a larger storm surge. The influence of coastal morphology was most prominent for TCs with a central pressure lower than 956 hPa. Tropical cyclone (TC) operational forecasts are continuously improving; however, there is still scope to improve the precision of storm surge predictions. These findings could contribute to the improvement of storm surge forecasting and provide emergency management personnel with more precise early warnings of dangerous storm surges.
Environmental monitoring and assessment, Jan 8, 2018
In this research, heavy metal accumulation pattern was investigated using the data measured from ... more In this research, heavy metal accumulation pattern was investigated using the data measured from the soil, paddy plants, and irrigation water samples in Jessore district in Bangladesh with the aid of principal component analysis. A total of 28 samples representing farmland soil and irrigation water along with paddy plant were collected from 28 locations in the Jessore district in November 2016. In agricultural soil, arsenic (As) and nickel (Ni) were found 2.78 and 1.11 times more concentrated than their background values. In addition, 89% of the sample sites exhibited enhanced As concentrations relative to the background value. Principal component analysis (PCA) of soil data showed strong homogeneity in many species (e.g., Ni, Cu, Fe, and As) to reflect intense agricultural activities. In contrast, Pb showed no such homogeneity in soil accumulation pattern. In plant samples, Cu, Fe, and As were strongly correlated and homologous. This homology of pollution was in agreement with the ...
Journal of microbiology, biotechnology and food sciences, 2015
Lablab purpureus (L.) Sweet is one of the most important and nutritious vegetables as well as pul... more Lablab purpureus (L.) Sweet is one of the most important and nutritious vegetables as well as pulse in Bangladesh and is grown extensively all over the country. It suffers from various fungal infections, which reduce greatly its quality and quantity. Seed-borne and seed associated fungi are one of the factors of substantial damages of the bean genotypes. Seed- borne and seed associated fungi of eleven genotypes of L. purpureus (L.) Sweet and their various controlling methods were studied. From eleven bean seed samples, seven types of fungal pathogens were isolated and identified. The most predominant fungi species were Aspergillus spp. followed by Fusarium sp. and Rhizopus sp. Comparatively less frequent fungi were Penicillium sp., Curvularia sp., Colletotrichum sp. and Alternaria sp. Plant extracts (Lawsonia inermis, Azadirachta indica and Allium sativum), cow urine, hot water and chemical fungicides (Bavistin, redomil and dithane M-45) were used to observe the efficacy of them aga...
The aim of this study is to investigate the hypoglycemic and hypolipidemic effects of Coccinia co... more The aim of this study is to investigate the hypoglycemic and hypolipidemic effects of Coccinia cordifolia leaf and root powder on alloxan induced type 2 diabetic Long-Evans rats. Oral feeding of the C. cordifolia leaf and root powder slightly decreased serum total cholesterol, triglyceride levels and LDL-cholesterol as compared with leaf, root and standard drug. C. cordifolia leaf showed more significant (p < 0.05, 0.01 and 0.001) effect on blood glucose level when compared with root and standard drug (glibenclamide, 5 mg/kg). At the mean time, rats’ serum insulin level markedly increased; leaf does showed more significant (p < 0.05, 0.01 and 0.001) effect than glibenclamide control group and root powder feeding group. But C. cordifolia did not show any significant effect on HDL-cholesterol and liver glycogen after 21st day feeding. Thus, the results of the experimental study suggest that C. cordifolia possesses hypoglycemic and hypolipidemic effects and is able to ameliorate ...
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: Bacillus sp. is now broadly responsible for various human infection and food spoilage.... more Objective: Bacillus sp. is now broadly responsible for various human infection and food spoilage. At present, identification and molecular characterization of major types of Bacillus sp. are important for human being. Methods: Antibacterial activities of six different concentrations each of ethanol, ethyl acetate and chloroform extracts of Coccinia grandis (L.) leaf on soil bacteria were assessed on the basis of disk diffusion method and the zone of inhibition was measured in mm unit. Different Bacillus sp. was characterized by using 16S rDNA sequencing. Results: Six different types of Bacillus sp. were identified depending on 16S rDNA sequencing and NCBI database information. It has also been shown that the best extract was ethyl acetate due to its highest zone of inhibition (Paenibacillus sp. BF38 (23.0±0.41), Terribacillus sp. 3LF (21.0±0.00), Bacillus simplex (23.0±0.43), Bacillus cereus (23.5±0.70), Paenibacillus sp.L32 (22.5±0.37), Bacillus megaterium (23.0±0.04)) as compared to other two extracts. Conclusion: The efficacy of Coccinia grandis (L.) leaf extracts as natural antibacterial agent was found ethyl acetate extract > ethanol extract > chloroform extract.
Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged m... more Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged mycelial culture of Lentinus lepideus were studied and their effectiveness was compared with lipopolysaccharide (LPS). The influence of the LL-EX on macrophage cellular lysosomal enzyme activity was to stimulate up to 267%, 392%, and 464% at the level of 10, 50, and 100 microg/ml, respectively. When the LL-EX was further fractionated into LL-Fr.I and Fr.II by Sepharose CL-6B gel chromatography, the cellular lysosomal enzyme activity of LL-Fr.II (2.1- fold) was higher than Fr.I (1.2-fold). Moreover, both LL-Fr.I and Fr.II stimulated the cytokines IL-1beta, TNF-alpha, and IL-6 in macrophages. In mixed lymphocyte reaction, LL-Fr.I and Fr.II enhanced the splenocyte proliferation up to 1.2-fold and 1.4-fold (50 microg/ml), respectively, stimulating only T lymphocytes. The fractions of LL-EX not show any direct toxicity against human gastric adenocarcinoma cell (AGS). The molecular masses of LL...
Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. ... more Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate belonged to Streptomyces albidoflavus. Preliminary screening revealed that the isolate was active against fungi and bacteria. Self-directing optimization was employed to determine the best combination of parameters such as carbon and nitrogen source, pH and temperature. Nutritional and culture conditions for the production of antibiotics by this organism under shake-flask conditions were also optimized. Maltose (5%) and soytone (5%) were found to be the best carbon and nitrogen sources for the production of antibiotics by S. albidoflavus C247. Additionally, 62.89% mycelial growth inhibition was achieved when the organism was cultured at 30℃ and pH 6.5. Ethyl acetate (EtOAc) was the best extraction solvent for the isolation of the antibiotics, and 100 µg/ml of EtOAc extract was found to inhi...
Asian Pacific Journal of Tropical Biomedicine, 2012
ABSTRACT Genistein recognized as phytoestrogens is one of the most extensively studied isoflavone... more ABSTRACT Genistein recognized as phytoestrogens is one of the most extensively studied isoflavones. It comprises of significant portion of Asian diet including Japanese and Chinese cuisine in the form of Soy food products. Evidence showed that geinstein increases osteoblasts formation as well as decreases osteoclast production. It plays an important role in immunity; such as suppression of delayed hypersensitivity and increases host resistance to B16F10 tumor by proliferating cytotoxic T and NK cells. It also decreases the activity of lipoprotein lipase which in turn inhibits lipogenesis and prevents the uptake of glucose in type 2 diabetic in rats. Geinstein play important role in reproductive system where it regulates the productive of oestrogen and progesterone. Moreover Geinstein has the ability to inhibit the tumor and cancer cell proliferation. Numerous beneficial effect of Geinstein including cancer treatment and function in immunity, obesity, diabetes and reproductivity Geinstein proves the potentiality of phytoestrogens as a source of bioactive substance.
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