International Journal of Current Microbiology and Applied Sciences
The study aimed to see the ability of some actinomycetes in removing of some toxic heavy metal io... more The study aimed to see the ability of some actinomycetes in removing of some toxic heavy metal ions (Cd2+, Mn2+) and some aflatoxins (AFG1, AFG2, AFB1, AFB2) from contaminated water samples. Out of 57 isolates of actinomycetes were isolated from different locations of Egypt. The most five isolates removed studied heavy metals, (Cd2+, Mn2+) singly above 65% were selected to remove studied metal ions from binary mixtures. The highest removing percentage was recorded by A26 isolate which was removed 71.4 %, and 70.0 % from Cd2+, and Mn2+ respectively. The 16S rRNA analyses and phylogenetic data of A26 concluded that it was member of Streptomyces genus, A26 was deposited in Gene Bank Data base under accession number MW947066. The results of the dead biomass of Streptomyces albus A26 for Cd2+, and Mn2+ under the optimized conditions pH-8 at 40°C for 3 hours with 0.3% bio sorbent dosage was found (Cd2+79.5 ± 2.37%, Mn+ 2,81.6±2%). All studied heavy metals were removed from aqueous solutio...
Carnosic acid (CA) is a natural phenolic compound with several biomedical actions. This work was ... more Carnosic acid (CA) is a natural phenolic compound with several biomedical actions. This work was performed to study the use of CA-loaded polymeric nanoparticles to improve the antitumor activity of breast cancer cells (MCF-7) and colon cancer cells (Caco-2). CA was encapsulated in bovine serum albumin (BSA), chitosan (CH), and cellulose (CL) nanoparticles. The CA-loaded BSA nanoparticles (CA-BSA-NPs) revealed the most promising formula as it showed good loading capacity and the best release rate profile as the drug reached 80% after 10 h. The physicochemical characterization of the CA-BSA-NPs and empty carrier (BSA-NPs) was performed by the particle size distribution analysis, transmission electron microscopy (TEM), and zeta potential. The antitumor activity of the CA-BSA-NPs was evaluated by measuring cell viability, apoptosis rate, and gene expression of GCLC, COX-2, and BCL-2 in MCF-7 and Caco-2. The cytotoxicity assay (MTT) showed elevated antitumor activity of CA-BSA-NPs agains...
Solenostemma argel is a desert medicinal plant indigenous to African countries. The goal of this ... more Solenostemma argel is a desert medicinal plant indigenous to African countries. The goal of this work was to investigate the pharmacological properties of phenolic and flavonoid compounds present in the Solenostemma argel. Aerial parts (leaves and flowers) of Solenostemma argel were tested for antibacterial activity, antioxidant activity, cell viability and cytotoxic effects, and anti-inflammatory activity. Phenolic and flavonoid contents (HPLC), and volatile constituents (GC-MS) were also characterized. The antioxidant activity of Solenostemma argel extract increased from (34.47%) at a concentration of 10 µg/mL to (98.47%) at a concentration of 1280 µg/mL. The most sensitive organism was S. aureus with the highest inhibition zone and lowest MIC, with acetone extract. Whereas C. tropicalis was the most resistant, with the lowest inhibition zone. MTT assay indicated that the methanolic extract of Solenostemma argel had significant cytotoxic effects on the A549, Caco-2, and MDAMB-231 ...
Euphorbia cuneata Vahl. (Euphorbiaceae) is a plant used in folk medicine for the treatment of pai... more Euphorbia cuneata Vahl. (Euphorbiaceae) is a plant used in folk medicine for the treatment of pain and inflammation, although the biological basis for these effects has not been thoroughly investigated. The goal of this study was to investigate the pharmacological properties and characterization of phenolic and flavonoid compounds present in the aerial parts of E. cuneata. E. cuneata aerial parts were tested for antioxidant activity (DPPH), antibacterial activity, cell viability and cytotoxic effects, and anti-inflammatory activity. Phenolic and flavonoid contents (HPLC), and volatile constituents (GC-MS) were also characterized. The methanol extract had the highest antioxidant activity, while the ether extract had the lowest. The antioxidant activity of E. cuneata extract increased from (21.11%) at a concentration of 10 µg/mL to (95.53%) at a concentration of 1280 µg/mL. S. aureus was the most sensitive organism with the highest zone of inhibition and lowest MIC, with acetone extra...
Background Paradoxically, patients with advanced stomach cancer who are Helicobacter pylori-posit... more Background Paradoxically, patients with advanced stomach cancer who are Helicobacter pylori-positive (HP+) have a higher survival rate than those who are HP-. This finding suggests that HP infection has beneficial effects for cancer treatment. Present study examines whether HP+ individuals have a lower likelihood of death from cancer than those who are HP-. Methods and findings Prospective cohort data (n = 4,982 subjects enrolled in the DAIKO study between 2008-2010) was used to assess whether anti-HP antibody status as a surrogate for past-present HP infection was associated with cancer incidence. The median age in the primary registry was 53 years-old (range 34-69 years-old). Over the 8-year observation period there were 234 (4.7%) cancer cases in the cohort and 88 (1.8%) all-cause deaths. Urine anti-HP antibody data was available for all but one participant (n = 4,981; 99.97%). The number of HP+ and HP- individuals was 1,826 (37%) and 3,156 (63%), respectively. Anti-HP antibody d...
This work aimed to detect the genetically modified potatoes in Egyptian market. DNA based method ... more This work aimed to detect the genetically modified potatoes in Egyptian market. DNA based method was used to detect genetically modified potatoes in the collected genotypes. Three primer pairs were selected to detect the genetic modification. The results indicated that 38 % of the investigated genotypes were positive for genetic modification in ac2 gene. Furthermore, 46% (12 Genotypes) were positive for the presence of genetically modified potato that contained CaMV promoter and cry V gene. In addition to 81% from the samples under investigation were positive for the presence of 35S promoter sequence in potatoes samples. From the obtained results we recommend the Egyptian government to establish strong regulations and certified laboratories to monitor GM crops or foods in the Egyptian market.
This chapter reviews the composition and functionality of cold pressed rosemary oil (CPRO). Fatty... more This chapter reviews the composition and functionality of cold pressed rosemary oil (CPRO). Fatty acids profile, lipid classes, tocols composition, and phenolic content are summarized. Antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and galvinoxyl free radicals, as well as antimicrobial potential against dermatophytic fungi, and food-borne bacteria are also reported. Linoleic and oleic acids were the main fatty acids, while linolenic acid was determined in lower amount. Tocopherols such as α- and γ-tocopherols accounted for 291 and 1145 mg/100 g CPRO, respectively. CPRO exhibited antiradical potential against DPPH• and galvinoxyl free radicals. CPRO showed antimicrobial activity against Escherichia coli, Salmonella enteritidis, and Listeria monocytogenes and antifungal potential against Trichophyton mentagrophytes and Trichophyton rubrum. Antidiabetic properties of CPRO in streptozotocin (STZ)-induced diabetic animals were reported considering the ameliorative eff...
International Journal of Current Microbiology and Applied Sciences
The study aimed to see the ability of some actinomycetes in removing of some toxic heavy metal io... more The study aimed to see the ability of some actinomycetes in removing of some toxic heavy metal ions (Cd2+, Mn2+) and some aflatoxins (AFG1, AFG2, AFB1, AFB2) from contaminated water samples. Out of 57 isolates of actinomycetes were isolated from different locations of Egypt. The most five isolates removed studied heavy metals, (Cd2+, Mn2+) singly above 65% were selected to remove studied metal ions from binary mixtures. The highest removing percentage was recorded by A26 isolate which was removed 71.4 %, and 70.0 % from Cd2+, and Mn2+ respectively. The 16S rRNA analyses and phylogenetic data of A26 concluded that it was member of Streptomyces genus, A26 was deposited in Gene Bank Data base under accession number MW947066. The results of the dead biomass of Streptomyces albus A26 for Cd2+, and Mn2+ under the optimized conditions pH-8 at 40°C for 3 hours with 0.3% bio sorbent dosage was found (Cd2+79.5 ± 2.37%, Mn+ 2,81.6±2%). All studied heavy metals were removed from aqueous solutio...
Carnosic acid (CA) is a natural phenolic compound with several biomedical actions. This work was ... more Carnosic acid (CA) is a natural phenolic compound with several biomedical actions. This work was performed to study the use of CA-loaded polymeric nanoparticles to improve the antitumor activity of breast cancer cells (MCF-7) and colon cancer cells (Caco-2). CA was encapsulated in bovine serum albumin (BSA), chitosan (CH), and cellulose (CL) nanoparticles. The CA-loaded BSA nanoparticles (CA-BSA-NPs) revealed the most promising formula as it showed good loading capacity and the best release rate profile as the drug reached 80% after 10 h. The physicochemical characterization of the CA-BSA-NPs and empty carrier (BSA-NPs) was performed by the particle size distribution analysis, transmission electron microscopy (TEM), and zeta potential. The antitumor activity of the CA-BSA-NPs was evaluated by measuring cell viability, apoptosis rate, and gene expression of GCLC, COX-2, and BCL-2 in MCF-7 and Caco-2. The cytotoxicity assay (MTT) showed elevated antitumor activity of CA-BSA-NPs agains...
Solenostemma argel is a desert medicinal plant indigenous to African countries. The goal of this ... more Solenostemma argel is a desert medicinal plant indigenous to African countries. The goal of this work was to investigate the pharmacological properties of phenolic and flavonoid compounds present in the Solenostemma argel. Aerial parts (leaves and flowers) of Solenostemma argel were tested for antibacterial activity, antioxidant activity, cell viability and cytotoxic effects, and anti-inflammatory activity. Phenolic and flavonoid contents (HPLC), and volatile constituents (GC-MS) were also characterized. The antioxidant activity of Solenostemma argel extract increased from (34.47%) at a concentration of 10 µg/mL to (98.47%) at a concentration of 1280 µg/mL. The most sensitive organism was S. aureus with the highest inhibition zone and lowest MIC, with acetone extract. Whereas C. tropicalis was the most resistant, with the lowest inhibition zone. MTT assay indicated that the methanolic extract of Solenostemma argel had significant cytotoxic effects on the A549, Caco-2, and MDAMB-231 ...
Euphorbia cuneata Vahl. (Euphorbiaceae) is a plant used in folk medicine for the treatment of pai... more Euphorbia cuneata Vahl. (Euphorbiaceae) is a plant used in folk medicine for the treatment of pain and inflammation, although the biological basis for these effects has not been thoroughly investigated. The goal of this study was to investigate the pharmacological properties and characterization of phenolic and flavonoid compounds present in the aerial parts of E. cuneata. E. cuneata aerial parts were tested for antioxidant activity (DPPH), antibacterial activity, cell viability and cytotoxic effects, and anti-inflammatory activity. Phenolic and flavonoid contents (HPLC), and volatile constituents (GC-MS) were also characterized. The methanol extract had the highest antioxidant activity, while the ether extract had the lowest. The antioxidant activity of E. cuneata extract increased from (21.11%) at a concentration of 10 µg/mL to (95.53%) at a concentration of 1280 µg/mL. S. aureus was the most sensitive organism with the highest zone of inhibition and lowest MIC, with acetone extra...
Background Paradoxically, patients with advanced stomach cancer who are Helicobacter pylori-posit... more Background Paradoxically, patients with advanced stomach cancer who are Helicobacter pylori-positive (HP+) have a higher survival rate than those who are HP-. This finding suggests that HP infection has beneficial effects for cancer treatment. Present study examines whether HP+ individuals have a lower likelihood of death from cancer than those who are HP-. Methods and findings Prospective cohort data (n = 4,982 subjects enrolled in the DAIKO study between 2008-2010) was used to assess whether anti-HP antibody status as a surrogate for past-present HP infection was associated with cancer incidence. The median age in the primary registry was 53 years-old (range 34-69 years-old). Over the 8-year observation period there were 234 (4.7%) cancer cases in the cohort and 88 (1.8%) all-cause deaths. Urine anti-HP antibody data was available for all but one participant (n = 4,981; 99.97%). The number of HP+ and HP- individuals was 1,826 (37%) and 3,156 (63%), respectively. Anti-HP antibody d...
This work aimed to detect the genetically modified potatoes in Egyptian market. DNA based method ... more This work aimed to detect the genetically modified potatoes in Egyptian market. DNA based method was used to detect genetically modified potatoes in the collected genotypes. Three primer pairs were selected to detect the genetic modification. The results indicated that 38 % of the investigated genotypes were positive for genetic modification in ac2 gene. Furthermore, 46% (12 Genotypes) were positive for the presence of genetically modified potato that contained CaMV promoter and cry V gene. In addition to 81% from the samples under investigation were positive for the presence of 35S promoter sequence in potatoes samples. From the obtained results we recommend the Egyptian government to establish strong regulations and certified laboratories to monitor GM crops or foods in the Egyptian market.
This chapter reviews the composition and functionality of cold pressed rosemary oil (CPRO). Fatty... more This chapter reviews the composition and functionality of cold pressed rosemary oil (CPRO). Fatty acids profile, lipid classes, tocols composition, and phenolic content are summarized. Antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and galvinoxyl free radicals, as well as antimicrobial potential against dermatophytic fungi, and food-borne bacteria are also reported. Linoleic and oleic acids were the main fatty acids, while linolenic acid was determined in lower amount. Tocopherols such as α- and γ-tocopherols accounted for 291 and 1145 mg/100 g CPRO, respectively. CPRO exhibited antiradical potential against DPPH• and galvinoxyl free radicals. CPRO showed antimicrobial activity against Escherichia coli, Salmonella enteritidis, and Listeria monocytogenes and antifungal potential against Trichophyton mentagrophytes and Trichophyton rubrum. Antidiabetic properties of CPRO in streptozotocin (STZ)-induced diabetic animals were reported considering the ameliorative eff...
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