Shoot tips of 2-3 cm long of two pomegranate cultivars (Manfalouty and Nab El-Gamal) were culture... more Shoot tips of 2-3 cm long of two pomegranate cultivars (Manfalouty and Nab El-Gamal) were cultured on three different media at full strength, namely Murashige and Skoog; (MS), Nitsch & Nitsch; (NN) and Woody Plant Medium; (WPM). Media were prepared as a basal medium supplemented with 3 GA at 5.0 mg/l, BA at 0.1 mg/l, IBA at 0.02 mg/l and activated charcoal at 3.0 g/l for establishment stage. For proliferation stage, BA and kinetin were tested at 0.0, 1.0 and 2.0 mg/l. For rooting stage, two different auxins; IBA and NAA were tested at 0.0, 0.25 and 0.5 mg/l on WPM at full (FSW) and half (HSW) strength. The plantlets grown on WPM were found to be significantly better in average survival (100 and 60%), plantlet height (5.10 and 4.58 cm), and average leaves number per shoot (11.3 and 10.0) for Manfalouty and Nab El-Gamal pomegranate cvs., respectively compared to other media. The two cultivars grown on WPM containing 1.0 mg/l BA had significantly the highest proliferation rate (6.8 and...
Shoot tips of 2-3 cm long of two pomegranate cultivars (Manfalouty and Nab El-Gamal) were culture... more Shoot tips of 2-3 cm long of two pomegranate cultivars (Manfalouty and Nab El-Gamal) were cultured on three different media at full strength, namely Murashige and Skoog; (MS), Nitsch & Nitsch; (NN) and Woody Plant Medium; (WPM). Media were prepared as a basal medium supplemented with 3 GA at 5.0 mg/l, BA at 0.1 mg/l, IBA at 0.02 mg/l and activated charcoal at 3.0 g/l for establishment stage. For proliferation stage, BA and kinetin were tested at 0.0, 1.0 and 2.0 mg/l. For rooting stage, two different auxins; IBA and NAA were tested at 0.0, 0.25 and 0.5 mg/l on WPM at full (FSW) and half (HSW) strength. The plantlets grown on WPM were found to be significantly better in average survival (100 and 60%), plantlet height (5.10 and 4.58 cm), and average leaves number per shoot (11.3 and 10.0) for Manfalouty and Nab El-Gamal pomegranate cvs., respectively compared to other media. The two cultivars grown on WPM containing 1.0 mg/l BA had significantly the highest proliferation rate (6.8 and...
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