R & D Establishment (Engrs), DRDO, Pune 411015 Email: jaipednekar@rde.drdo.in UV-LIGA process is ... more R & D Establishment (Engrs), DRDO, Pune 411015 Email: jaipednekar@rde.drdo.in UV-LIGA process is seen as cheaper alternative for fabrication of precision micromachined components. With advancement in MEMS technology, it has become possible to develop such complex micromechanical systems. Out of various fabrication processes developed and established under MEMS technology, UV-LIGA process offers distinct advantage for manufacturing of such components over conventional machining such as WEDM or Laser micromachining. This paper reports development of a customized UV-LIGA process for fabrication of leaf spring for use in complex mechanical assembly.
Genomic sequences of Helicobacter pylori strains 26695, J99, HPAGI and G27 have revealed an abund... more Genomic sequences of Helicobacter pylori strains 26695, J99, HPAGI and G27 have revealed an abundance of restriction and modification genes. hp0050, which encodes an N(6) adenine DNA methyltransferase, was cloned, overexpressed and purified to near homogeneity. It recognizes the sequence 5'-GRRG-3' (where R is A or G) and, most intriguingly, methylates both adenines when R is A (5'-GAAG-3'). Kinetic analysis suggests a nonprocessive (repeated-hit) mechanism of methylation in which HP0050 methyltransferase methylates one adenine at a time in the sequence 5'-GAAG-3'. This is the first report of an N(6) adenine DNA methyltransferase that methylates two adjacent residues on the same strand. Interestingly, HP0050 homologs from two clinical strains of H. pylori (PG227 and 128) methylate only…
A man presented with multifocal pancreatic metastases 9 years after nephrectomy for renal cell ca... more A man presented with multifocal pancreatic metastases 9 years after nephrectomy for renal cell carcinoma. He was managed with oral sunitinib. He had favourable response to treatment with excellent compliance.
A 26-year-old female presented with complaints of bleeding per rectum for 6 months. Per rectal ex... more A 26-year-old female presented with complaints of bleeding per rectum for 6 months. Per rectal examination revealed growth in lower third of rectum. Complete gynecological examination was performed followed by cytological analysis of uterine cervix cell smear and all findings were normal. Lower gastrointestinal endoscopy (LGIE) was suggestive of 5 cm ulcerative growth in the rectal area. Contrast-enhanced computed tomography (CECT) showed circumferential thickening of rectal wall with a length of 5 cm with an eccentric growth on right rectal wall with maximum wall thickness of 10 mm. Biopsy was suggestive of moderately differentiated mucin-secreting adenocarcinoma. Hence, patient was diagnosed with rectal cancer. She was treated with neoadjuvant chemoradiation with 45 Gy in 25 fractions and weekly chemotherapy with 5-FU and leucovorin. The patient then underwent low anterior resection with total mesorectal excision. Pathological examination found a moderately differentiated adenocarcinomawith serosal involvement. Perineural spread and lymphovascular emboli were present; however, lymph nodes dissected were free of tumor. She was pathologically staged as pT4aN0M0. She was then treated with six cycles of adjuvant chemotherapy with FOLFOX. CECT at 1-month post-completion of chemotherapy and at 4 months did not reveal any abnormality. Eight months later, she camewith complaints of postcoital bleeding and leucorrhea. On pelvic examination, an ulceroproliferative growth was found at cervix which was involving all fornices and upper half of all vaginal walls. Biopsy was suggestive of metastatic adenocarcinoma with mucin-secreting features. Immunohistochemistry (IHC) performed showed positivity of tumor cells for CK20 and negative for CK7, confirming metastasis from colorectal primary (Fig. 1a). PET-CT showed a lesion in the cervix with parametrial infiltration and loss of fat planes between lesion and bladder. As the patient was not a candidate for surgery in view of the involvement of vaginal walls, so she was started on secondline chemotherapy with FOLFIRI regimen. Chemotherapy was stopped after three cycles due to progression of disease and intolerance. The patient died of progressive disease.
Chronic fatigue syndrome, or CFS, is a severe, incapacitating fatigue lasting for six months or m... more Chronic fatigue syndrome, or CFS, is a severe, incapacitating fatigue lasting for six months or more, that is not improved by bed rest and that may be exacerbated by physical or mental activity. It is an all-encompassing fatigue that results in a dramatic decline in both activity level and stamina. This syndrome is not new and has a long history. CFS is diagnosed on the basis of symptoms. Although pathalophysiological abnormalities exist across many domain, but several lines of research suggest that it may be distinct from psychiatric disorder. The nature of the symptoms, however, can help clinicians differentiate CFS from other illnesses. Center of Diagnosis has developed diagnostic criteria as based upon symptoms & exclusion of other illness. Treatment of CFS is sympom based and include pharmacological, cognitive and behavioral therapy, with lifestyle exercise and lifestyle management therefore the assesment and treatment of CFS should be multidirectional and taliored to the need of patients.
Trypanosoma evansi is the most extensively distributed trypanosome responsible for disease called... more Trypanosoma evansi is the most extensively distributed trypanosome responsible for disease called surra in livestock in many countries including frequent outbreaks in India. The prevalence of this disease is most commonly reported by standard parasitological detection methods (SPDM); however, antibody ELISA is being in practice by locally produced whole cell lysate (WCL) antigens in many countries. In the present investigation, we attempted to identify and purify immuno dominant, infection specific trypanosome antigens from T. evansi proteome using experimentally infected equine serum by immuno blot. Three immuno dominant clusters of proteins i.e. 62-66 kDa, 52-55 kDa and 41-43 kDa were identified based on their consistent reactivity with donkey sequential serum experimentally infected T. evansi up to 280 days post infection (dpi). The protein cluster of 62-66 kDa was purified in bulk in native form and comparatively evaluated with whole cell lysate antigen (WCL). ELISA and immuno blot showed that polypeptide of this cluster is 100% sensitive in detection of early and chronic infection. Further, this protein cluster was also found immuno reactive against hyper immune serum raised against predominantly 66 kDa exo antigen, revealed that this is a common immunodominant moieties in proteome and secretome of T. evansi.
www.thelancet.com Vol 380 October 13, 2012 1303 Submissions should be made via our electronic sub... more www.thelancet.com Vol 380 October 13, 2012 1303 Submissions should be made via our electronic submission system at http://ees.elsevier.com/ thelancet/ measles elimination. China, with 75% of the regional population, reached more than 103 million people in a measles campaign in September, 2010, with a substantial decline in measles incidence from 28·5 in 2010 to 2·5 per million population in the fi rst quarter of 2012. The Western Pacifi c region has thus recently established a verifi cation commission and mechanism. The remarkable achievements in the Americas region and progress in the Western Pacifi c region clearly show the feasibility of eliminating measles. The global community must reinvigorate measles elimination eff orts to ensure that proven strategies are robustly and universally implemented. It is unconscionable that children should continue to die as a result of measles during the 21st century.
R & D Establishment (Engrs), DRDO, Pune 411015 Email: jaipednekar@rde.drdo.in UV-LIGA process is ... more R & D Establishment (Engrs), DRDO, Pune 411015 Email: jaipednekar@rde.drdo.in UV-LIGA process is seen as cheaper alternative for fabrication of precision micromachined components. With advancement in MEMS technology, it has become possible to develop such complex micromechanical systems. Out of various fabrication processes developed and established under MEMS technology, UV-LIGA process offers distinct advantage for manufacturing of such components over conventional machining such as WEDM or Laser micromachining. This paper reports development of a customized UV-LIGA process for fabrication of leaf spring for use in complex mechanical assembly.
Genomic sequences of Helicobacter pylori strains 26695, J99, HPAGI and G27 have revealed an abund... more Genomic sequences of Helicobacter pylori strains 26695, J99, HPAGI and G27 have revealed an abundance of restriction and modification genes. hp0050, which encodes an N(6) adenine DNA methyltransferase, was cloned, overexpressed and purified to near homogeneity. It recognizes the sequence 5'-GRRG-3' (where R is A or G) and, most intriguingly, methylates both adenines when R is A (5'-GAAG-3'). Kinetic analysis suggests a nonprocessive (repeated-hit) mechanism of methylation in which HP0050 methyltransferase methylates one adenine at a time in the sequence 5'-GAAG-3'. This is the first report of an N(6) adenine DNA methyltransferase that methylates two adjacent residues on the same strand. Interestingly, HP0050 homologs from two clinical strains of H. pylori (PG227 and 128) methylate only…
A man presented with multifocal pancreatic metastases 9 years after nephrectomy for renal cell ca... more A man presented with multifocal pancreatic metastases 9 years after nephrectomy for renal cell carcinoma. He was managed with oral sunitinib. He had favourable response to treatment with excellent compliance.
A 26-year-old female presented with complaints of bleeding per rectum for 6 months. Per rectal ex... more A 26-year-old female presented with complaints of bleeding per rectum for 6 months. Per rectal examination revealed growth in lower third of rectum. Complete gynecological examination was performed followed by cytological analysis of uterine cervix cell smear and all findings were normal. Lower gastrointestinal endoscopy (LGIE) was suggestive of 5 cm ulcerative growth in the rectal area. Contrast-enhanced computed tomography (CECT) showed circumferential thickening of rectal wall with a length of 5 cm with an eccentric growth on right rectal wall with maximum wall thickness of 10 mm. Biopsy was suggestive of moderately differentiated mucin-secreting adenocarcinoma. Hence, patient was diagnosed with rectal cancer. She was treated with neoadjuvant chemoradiation with 45 Gy in 25 fractions and weekly chemotherapy with 5-FU and leucovorin. The patient then underwent low anterior resection with total mesorectal excision. Pathological examination found a moderately differentiated adenocarcinomawith serosal involvement. Perineural spread and lymphovascular emboli were present; however, lymph nodes dissected were free of tumor. She was pathologically staged as pT4aN0M0. She was then treated with six cycles of adjuvant chemotherapy with FOLFOX. CECT at 1-month post-completion of chemotherapy and at 4 months did not reveal any abnormality. Eight months later, she camewith complaints of postcoital bleeding and leucorrhea. On pelvic examination, an ulceroproliferative growth was found at cervix which was involving all fornices and upper half of all vaginal walls. Biopsy was suggestive of metastatic adenocarcinoma with mucin-secreting features. Immunohistochemistry (IHC) performed showed positivity of tumor cells for CK20 and negative for CK7, confirming metastasis from colorectal primary (Fig. 1a). PET-CT showed a lesion in the cervix with parametrial infiltration and loss of fat planes between lesion and bladder. As the patient was not a candidate for surgery in view of the involvement of vaginal walls, so she was started on secondline chemotherapy with FOLFIRI regimen. Chemotherapy was stopped after three cycles due to progression of disease and intolerance. The patient died of progressive disease.
Chronic fatigue syndrome, or CFS, is a severe, incapacitating fatigue lasting for six months or m... more Chronic fatigue syndrome, or CFS, is a severe, incapacitating fatigue lasting for six months or more, that is not improved by bed rest and that may be exacerbated by physical or mental activity. It is an all-encompassing fatigue that results in a dramatic decline in both activity level and stamina. This syndrome is not new and has a long history. CFS is diagnosed on the basis of symptoms. Although pathalophysiological abnormalities exist across many domain, but several lines of research suggest that it may be distinct from psychiatric disorder. The nature of the symptoms, however, can help clinicians differentiate CFS from other illnesses. Center of Diagnosis has developed diagnostic criteria as based upon symptoms & exclusion of other illness. Treatment of CFS is sympom based and include pharmacological, cognitive and behavioral therapy, with lifestyle exercise and lifestyle management therefore the assesment and treatment of CFS should be multidirectional and taliored to the need of patients.
Trypanosoma evansi is the most extensively distributed trypanosome responsible for disease called... more Trypanosoma evansi is the most extensively distributed trypanosome responsible for disease called surra in livestock in many countries including frequent outbreaks in India. The prevalence of this disease is most commonly reported by standard parasitological detection methods (SPDM); however, antibody ELISA is being in practice by locally produced whole cell lysate (WCL) antigens in many countries. In the present investigation, we attempted to identify and purify immuno dominant, infection specific trypanosome antigens from T. evansi proteome using experimentally infected equine serum by immuno blot. Three immuno dominant clusters of proteins i.e. 62-66 kDa, 52-55 kDa and 41-43 kDa were identified based on their consistent reactivity with donkey sequential serum experimentally infected T. evansi up to 280 days post infection (dpi). The protein cluster of 62-66 kDa was purified in bulk in native form and comparatively evaluated with whole cell lysate antigen (WCL). ELISA and immuno blot showed that polypeptide of this cluster is 100% sensitive in detection of early and chronic infection. Further, this protein cluster was also found immuno reactive against hyper immune serum raised against predominantly 66 kDa exo antigen, revealed that this is a common immunodominant moieties in proteome and secretome of T. evansi.
www.thelancet.com Vol 380 October 13, 2012 1303 Submissions should be made via our electronic sub... more www.thelancet.com Vol 380 October 13, 2012 1303 Submissions should be made via our electronic submission system at http://ees.elsevier.com/ thelancet/ measles elimination. China, with 75% of the regional population, reached more than 103 million people in a measles campaign in September, 2010, with a substantial decline in measles incidence from 28·5 in 2010 to 2·5 per million population in the fi rst quarter of 2012. The Western Pacifi c region has thus recently established a verifi cation commission and mechanism. The remarkable achievements in the Americas region and progress in the Western Pacifi c region clearly show the feasibility of eliminating measles. The global community must reinvigorate measles elimination eff orts to ensure that proven strategies are robustly and universally implemented. It is unconscionable that children should continue to die as a result of measles during the 21st century.
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