The effects of endurance exercise on muscle, and adipose tissue metabolism were investigated. Fem... more The effects of endurance exercise on muscle, and adipose tissue metabolism were investigated. Female lean Zucker rats swam for two hours at high intensity. Three groups were examined: pre-exercise control (C), exercised (E) and 24 hours post-exercise (E-24). Exercise increased fat cell lipolysis in the inguinal depot (p less than 0.05) while no effect was detected in the parametrial depot. In contrast, parametrial pad lipoprotein lipase (LPL) activity decreased after exercise with 24 hours post-exercise values being reduced below E and C rats (p less than 0.05). Gastrocnemius LPL activity remained unchanged during exercise while heart LPL increased, E having higher values than C and E-24 (p less than 0.05). Gastrocnemius, but not heart, citrate synthase activity increased with exercise, with E-24 values increased compared to E and C (p less than 0.05). These results demonstrate that adipose tissue's response to exercise is site specific, and suggests a distinct physiological role for different adipose depots. Muscle LPL and citrate synthase activities were modified differently for gastrocnemius and heart, confirming the distinct metabolic response to exercise of these two muscles.
The effects of endurance exercise training on adipose tissue have been investigated in female lea... more The effects of endurance exercise training on adipose tissue have been investigated in female lean Zucker rats. Adult trained rats (TR) were followed throughout a swimming program of 5 wk and were compared with a littermate control sedentary group (SED). Data were collected on days 0, 14, 24, and 36 of the training program. Body weight gain and cumulative food intake were significantly lower in TR than in SED (P less than 0.05). Gastrocnemius citrate synthase activity was increased in TR by day 14 of training (P less than 0.05) and was followed by a second significant increase between days 24 and 36 (P less than 0.05). Although inguinal (ING), parametrial (PAR), and retroperitoneal (RP) cell sizes were decreased by the swimming program (P less than 0.05), adipose tissue lipoprotein lipase (LPL) activity was suppressed (P less than 0.05) by training during the first 24 days in PAR and RP depots only. Thereafter, PAR and RP LPL activities increased in TR animals (P less than 0.05) to ...
To study the influence of endurance training on glucose conversion into fat cell triglycerides, 2... more To study the influence of endurance training on glucose conversion into fat cell triglycerides, 24 (13 women, 11 men) inactive subjects (25.0 +/- 3.8 yr of age) took part in a 20-wk ergocycle training program 4 days and increasing to 5 days/wk, 40–45 min/day, starting at 60% and increasing to 85% of the heart rate reserve. Several body fatness indicators were measured before and after the training program: seven skinfold thicknesses, percent fat, and mean fat cell weight. Fat cell basal and maximal insulin-stimulated glucose conversion into triglycerides were also determined using [14C]glucose. Body fatness indicators decreased significantly after training only in male subjects (P less than 0.05). Basal and maximal insulin-stimulated glucose conversion into triglycerides increased significantly with training (P less than 0.05): pretest values (nanomoles glucose per hour per 10(6) cells) being 24.9 +/- 2.1 and 28.7 +/- 2.5, while post-test values were 30.1 +/- 3.2 and 33.0 +/- 3.4 fo...
Acta geneticae medicae et gemellologiae: twin research, 1985
In order to study the influence of heredity on resting metabolic rate (RMR), 20 monozygotic and 1... more In order to study the influence of heredity on resting metabolic rate (RMR), 20 monozygotic and 19 dizygotic male twin pairs aged 20.6 (SD 2.9) and 21.4 (SD 3.1) years, gave their consent to participate in the experiment. Fat free weight (FFW) was estimated from underwater weighing. RMR was measured by indirect calorimetry using an open circuit system. RMR was expressed as kJ · min−1, kJ/m2· h−1, kJ/kg · h−1and kJ/kgFFW · h−1. Significant intraclass coefficients were observed in MZ twins for the different expressions of RMR. The values ranged from r = 0.45 (P < 0.05) to r = 0.81 (P < 0.01). However, DZ twins demonstrated lower intraclass coefficients for RMR, with a range from r = 0.21 to r = 0.44. Significant (P < 0.05) DZ resemblance was revealed only when RMR was expressed as kJ · min−1and kJ/kg · h−1. Results of the present study suggest that variations in RMR may have a genetic component. Implications for human energy balance and body fat are discussed.
Twenty-three male subjects, 31.7 (mean) +/- 7.4 (s.d.) yr of age, weight 70.7 +/- 9.0 kg and with... more Twenty-three male subjects, 31.7 (mean) +/- 7.4 (s.d.) yr of age, weight 70.7 +/- 9.0 kg and with 13.4 +/- 4.7 percent body fat were submitted twice within a week to biopsies of subcutaneous adipose tissue in the suprailiac region. Two biopsies (sample A and B) were obtained on day 1 and two others (sample C and D) in the contralateral region on day 2. Average fat cell diameter (ACD) was assessed by measuring at least 500 cells per subject. There was no significant difference between day 1 and day 2 for ACD (means 1 = 62.6 +/- 12.2 micron; means 2 = 63.2 +/- 12.3 micron). Intra-class reliability coefficient reached 0.97. ANOVA-RBFD showed no day or sample effects (P greater than 0.05) on ACD. Basal (BL), epinephrine submaximal (10(-5) M) (ESML) and epinephrine maximal (10(-4) M) (EML) stimulated lipolysis have been determined for 18 of these subjects. Intra-class reliabilities were moderately high, reaching 0.71 for BL, 0.83 for ESML and 0.72 for EML, respectively. Controlling the l...
The present experiment was conducted to study the influence of exercise training on adipose tissu... more The present experiment was conducted to study the influence of exercise training on adipose tissue lipolytic activity and to identify the amount of training required to induce maximal adaptation in humans. Fifty-one male subjects were divided into three groups according to their training regimen: 1) sedentary subjects (SS) (n = 21); 2) trained subjects (TS) (n = 15) who had exercised during a period of 20 wk, 5 days/wk, 45 min/session; and 3) experienced marathon runners (MR) (n = 15) who ran an average of 120 km/wk for many years. Biopsies of fat were performed in the suprailiac region after an overnight fast. Adipocyte diameter (AD) and epinephrine-stimulated lipolysis ( ESL ) were assessed on collagenase-isolated fat cells. A lower AD was noted in the MR group compared with the two other groups. Basal lipolysis (BL) and ESL were significantly higher in TS and MR than in controls. Moreover, BL values were comparable in the two trained groups, whereas ESL in the TS group was higher...
In order to study the effects of heredity and of physical training on adipose tissue morphology a... more In order to study the effects of heredity and of physical training on adipose tissue morphology and metabolism, 15 pairs of monozygotic twins (MZ) (six males and nine females), aged from 16 to 24 years, weighing 56.2 +/- 9.1 kg and with 13.9 +/- 8.2 percent body fat, were submitted to a biopsy of adipose tissue in the suprailiac region. In addition, eight pairs of twins (four male and four female) took part in a 20-week ergocycle training program, five days a week, 40 min a day, at 80 percent of their maximal heart rate. Adipocyte diameter (AD) was assessed on collagenase isolated fat cells. Basal (BL), epinephrine submaximal (10(-5) M) (ESML) and epinephrine maximal stimulated lipolysis (10(-4) M) (EML) were determined on isolated fat cells. Intraclass correlation coefficients indicated significant intrapair resemblance before training for all fat morphological and metabolic indicators (0.78 less than or equal to ri less than or equal to 0.93). Training significantly increased VO2 ...
Journal of Toxicology and Environmental Health, 1994
The possible interaction between intense exercise, known to suppress the immune response, and nut... more The possible interaction between intense exercise, known to suppress the immune response, and nutritive factors, such as polyunsaturated fatty acids (PUFA), was examined in inbred female C57Bl/6 mice. The animals received for 8 wk either a natural ingredient diet or a diet supplemented with 10 g/100 g linseed oil containing over 50% of 18:3 (n-3) alpha-linoleic acid. Other groups received PUFA containing only traces of 18:3 (n-3) fatty acid; beef tallow, containing mostly 18:1 (n-9) saturated fat, safflower oil, an 18:2 (n-6) PUFA, and fish oil, containing longer chain (n-3) PUFA. Each dietary group was divided into two subgroups: sedentary diet controls and exercised animals. Exercise consisted of continuous swimming at high intensity until exhaustion. It was shown in three separate experiments that (1) the primary humoral response to sheep red blood cells, determined by the plaque-forming cell (PFC) assay, was affected by PUFA diet in sedentary animals in the order beef tallow &amp;amp;gt; control diet &amp;amp;gt; safflower oil &amp;amp;gt; fish oil &amp;amp;gt; linseed oil, and (2) the PFC response was suppressed by the exhaustive exercise, as compared to sedentary controls, except for animals fed 18:3 (n-3) linseed oil, where the normal response was noted. Phagocytosis of fluorescent microspheres by peritoneal macrophages, determined by flow cytometry, was significantly lower in exercised animals receiving the linseed oil diet, whereas other diets either increased or did not significantly change the macrophage phagocytic activity, compared to the sedentary diet controls. Spleen lymphocyte subsets were unchanged in exercised animals except for a marked shift from the lymphoid peak toward the erythroid peak. Generally, our data showed a marked immunomodulatory effect of 18-3 (n-3) alpha-linoleic acid on the exhaustive exercise-related immunosuppression, as compared to the effects of other selected PUFA.
Eighteen male marathon runners (mean marathon performance: 2 h 36 min, SD = 7.0 min; VO2 max = 64... more Eighteen male marathon runners (mean marathon performance: 2 h 36 min, SD = 7.0 min; VO2 max = 64.1 +/- 15.1 ml/kg . min-1) were submitted to a needle biopsy in the suprailiac region and to various measurements of body fatness: percent body fat (% fat), seven skinfold thicknesses, and mean fat cell diameter. Basal and insulin-stimulated glucose conversion into triglycerides were measured in collagenase-isolated fat cells, while heparin-releasable lipoprotein lipase activity (LPLa) was determined in intact adipose tissue. All body fatness indicators were significantly smaller in marathon runners in comparison to a sedentary control group (P less than 0.001). Fat cell basal and insulin-stimulated glucose conversion into triglycerides as well as LPLa were significantly higher for the runners group (P less than 0.01), differences being particularly important when comparisons were performed between subjects paired for mean fat cell diameter. Pearson interclass correlations between body fatness and fat cell glucose incorporation into triglycerides were low and positive for the sedentary group (0.04 less than or equal to r less than or equal to 0.41), while they were negative for the marathon runners groups (-0.28 less than or equal to r less than or equal to -0.40) with the exception of % fat. Moreover, correlations between LPLa and indicators of body fatness were positive in the sedentary group (0.47 less than or equal to r less than or equal to 0.79), while they were negative in the marathon runners group (-0.03 less than or equal to r less than or equal to -0.63).(ABSTRACT TRUNCATED AT 250 WORDS)
Minerals, such as calcium and potassium, are essential for fetal development, but their transplac... more Minerals, such as calcium and potassium, are essential for fetal development, but their transplacental transport, and in particular, the effect of hormones on this process has not been extensively studied. Human alpha-calcitonin gene-related peptide (h alpha CGRP), a hormone constituted of 37 amino acids, is obtained by the alternative splicing of the mRNA from the calcitonin gene, and could be implicated in placental ion transport. In order to study the presence of this receptor, brush-border and basal plasma membranes were purified, and membrane binding studies were conducted using [125I]h alpha CGRP. The initiation of binding of [125I]h alpha CGRP to both membranes was rapid and reached maximal value after 10 min of incubation at 37 degrees C. Scratchard analysis revealed single-affinity binding sites for h alpha CGRP with Kd equal to 4412.45 +/- 604.81 pM and 2673.24 +/- 552.51 pM for brush-border and basal plasma membranes, respectively, which were significantly different. Moreover, the maximal number of receptors was significantly different (P &lt; 0.001) in both membranes, with Bmax of 627.94 +/- 31.40 fmol/mg protein for brush-border membranes and 343.70 +/- 43.52 fmol/mg protein in basal-plasma membranes. Competitive displacement of [125I]h alpha CGRP with other ligands showed the following potencies; h alpha CGRP approximately h beta CGRP approximately Cys (acm)2,7 CGRP &gt; CGRP (8-37), but no competition was observed with human and salmon calcitonin. Half-maximal displacement for human alpha- and beta CGRP was reached at approximately 10(-10)M for brush-border and basal-plasma membranes. alpha- and beta CGRP, and their fragment and analogue, stimulated cyclic AMP production in placental homogenate ranging from 143-163 per cent. Thus, our results show the presence of CGRP-specific receptors in both the syncytiotrophoblast membranes of human placenta. The role(s) of this related peptide in placenta remains to be investigated.
... Procedure for the determination of body density was as described by Behnke and Wilmore.&a... more ... Procedure for the determination of body density was as described by Behnke and Wilmore.&amp;quot; Percent fat was estimated from body density according to Siri.&amp;quot; The method of Wilmore et al&amp;#x27;8 was employed for the ... Collagenase was obtained from Worthington Laboratories. ...
The effects of endurance exercise on muscle, and adipose tissue metabolism were investigated. Fem... more The effects of endurance exercise on muscle, and adipose tissue metabolism were investigated. Female lean Zucker rats swam for two hours at high intensity. Three groups were examined: pre-exercise control (C), exercised (E) and 24 hours post-exercise (E-24). Exercise increased fat cell lipolysis in the inguinal depot (p less than 0.05) while no effect was detected in the parametrial depot. In contrast, parametrial pad lipoprotein lipase (LPL) activity decreased after exercise with 24 hours post-exercise values being reduced below E and C rats (p less than 0.05). Gastrocnemius LPL activity remained unchanged during exercise while heart LPL increased, E having higher values than C and E-24 (p less than 0.05). Gastrocnemius, but not heart, citrate synthase activity increased with exercise, with E-24 values increased compared to E and C (p less than 0.05). These results demonstrate that adipose tissue&#39;s response to exercise is site specific, and suggests a distinct physiological role for different adipose depots. Muscle LPL and citrate synthase activities were modified differently for gastrocnemius and heart, confirming the distinct metabolic response to exercise of these two muscles.
The effects of endurance exercise training on adipose tissue have been investigated in female lea... more The effects of endurance exercise training on adipose tissue have been investigated in female lean Zucker rats. Adult trained rats (TR) were followed throughout a swimming program of 5 wk and were compared with a littermate control sedentary group (SED). Data were collected on days 0, 14, 24, and 36 of the training program. Body weight gain and cumulative food intake were significantly lower in TR than in SED (P less than 0.05). Gastrocnemius citrate synthase activity was increased in TR by day 14 of training (P less than 0.05) and was followed by a second significant increase between days 24 and 36 (P less than 0.05). Although inguinal (ING), parametrial (PAR), and retroperitoneal (RP) cell sizes were decreased by the swimming program (P less than 0.05), adipose tissue lipoprotein lipase (LPL) activity was suppressed (P less than 0.05) by training during the first 24 days in PAR and RP depots only. Thereafter, PAR and RP LPL activities increased in TR animals (P less than 0.05) to ...
To study the influence of endurance training on glucose conversion into fat cell triglycerides, 2... more To study the influence of endurance training on glucose conversion into fat cell triglycerides, 24 (13 women, 11 men) inactive subjects (25.0 +/- 3.8 yr of age) took part in a 20-wk ergocycle training program 4 days and increasing to 5 days/wk, 40–45 min/day, starting at 60% and increasing to 85% of the heart rate reserve. Several body fatness indicators were measured before and after the training program: seven skinfold thicknesses, percent fat, and mean fat cell weight. Fat cell basal and maximal insulin-stimulated glucose conversion into triglycerides were also determined using [14C]glucose. Body fatness indicators decreased significantly after training only in male subjects (P less than 0.05). Basal and maximal insulin-stimulated glucose conversion into triglycerides increased significantly with training (P less than 0.05): pretest values (nanomoles glucose per hour per 10(6) cells) being 24.9 +/- 2.1 and 28.7 +/- 2.5, while post-test values were 30.1 +/- 3.2 and 33.0 +/- 3.4 fo...
Acta geneticae medicae et gemellologiae: twin research, 1985
In order to study the influence of heredity on resting metabolic rate (RMR), 20 monozygotic and 1... more In order to study the influence of heredity on resting metabolic rate (RMR), 20 monozygotic and 19 dizygotic male twin pairs aged 20.6 (SD 2.9) and 21.4 (SD 3.1) years, gave their consent to participate in the experiment. Fat free weight (FFW) was estimated from underwater weighing. RMR was measured by indirect calorimetry using an open circuit system. RMR was expressed as kJ · min−1, kJ/m2· h−1, kJ/kg · h−1and kJ/kgFFW · h−1. Significant intraclass coefficients were observed in MZ twins for the different expressions of RMR. The values ranged from r = 0.45 (P < 0.05) to r = 0.81 (P < 0.01). However, DZ twins demonstrated lower intraclass coefficients for RMR, with a range from r = 0.21 to r = 0.44. Significant (P < 0.05) DZ resemblance was revealed only when RMR was expressed as kJ · min−1and kJ/kg · h−1. Results of the present study suggest that variations in RMR may have a genetic component. Implications for human energy balance and body fat are discussed.
Twenty-three male subjects, 31.7 (mean) +/- 7.4 (s.d.) yr of age, weight 70.7 +/- 9.0 kg and with... more Twenty-three male subjects, 31.7 (mean) +/- 7.4 (s.d.) yr of age, weight 70.7 +/- 9.0 kg and with 13.4 +/- 4.7 percent body fat were submitted twice within a week to biopsies of subcutaneous adipose tissue in the suprailiac region. Two biopsies (sample A and B) were obtained on day 1 and two others (sample C and D) in the contralateral region on day 2. Average fat cell diameter (ACD) was assessed by measuring at least 500 cells per subject. There was no significant difference between day 1 and day 2 for ACD (means 1 = 62.6 +/- 12.2 micron; means 2 = 63.2 +/- 12.3 micron). Intra-class reliability coefficient reached 0.97. ANOVA-RBFD showed no day or sample effects (P greater than 0.05) on ACD. Basal (BL), epinephrine submaximal (10(-5) M) (ESML) and epinephrine maximal (10(-4) M) (EML) stimulated lipolysis have been determined for 18 of these subjects. Intra-class reliabilities were moderately high, reaching 0.71 for BL, 0.83 for ESML and 0.72 for EML, respectively. Controlling the l...
The present experiment was conducted to study the influence of exercise training on adipose tissu... more The present experiment was conducted to study the influence of exercise training on adipose tissue lipolytic activity and to identify the amount of training required to induce maximal adaptation in humans. Fifty-one male subjects were divided into three groups according to their training regimen: 1) sedentary subjects (SS) (n = 21); 2) trained subjects (TS) (n = 15) who had exercised during a period of 20 wk, 5 days/wk, 45 min/session; and 3) experienced marathon runners (MR) (n = 15) who ran an average of 120 km/wk for many years. Biopsies of fat were performed in the suprailiac region after an overnight fast. Adipocyte diameter (AD) and epinephrine-stimulated lipolysis ( ESL ) were assessed on collagenase-isolated fat cells. A lower AD was noted in the MR group compared with the two other groups. Basal lipolysis (BL) and ESL were significantly higher in TS and MR than in controls. Moreover, BL values were comparable in the two trained groups, whereas ESL in the TS group was higher...
In order to study the effects of heredity and of physical training on adipose tissue morphology a... more In order to study the effects of heredity and of physical training on adipose tissue morphology and metabolism, 15 pairs of monozygotic twins (MZ) (six males and nine females), aged from 16 to 24 years, weighing 56.2 +/- 9.1 kg and with 13.9 +/- 8.2 percent body fat, were submitted to a biopsy of adipose tissue in the suprailiac region. In addition, eight pairs of twins (four male and four female) took part in a 20-week ergocycle training program, five days a week, 40 min a day, at 80 percent of their maximal heart rate. Adipocyte diameter (AD) was assessed on collagenase isolated fat cells. Basal (BL), epinephrine submaximal (10(-5) M) (ESML) and epinephrine maximal stimulated lipolysis (10(-4) M) (EML) were determined on isolated fat cells. Intraclass correlation coefficients indicated significant intrapair resemblance before training for all fat morphological and metabolic indicators (0.78 less than or equal to ri less than or equal to 0.93). Training significantly increased VO2 ...
Journal of Toxicology and Environmental Health, 1994
The possible interaction between intense exercise, known to suppress the immune response, and nut... more The possible interaction between intense exercise, known to suppress the immune response, and nutritive factors, such as polyunsaturated fatty acids (PUFA), was examined in inbred female C57Bl/6 mice. The animals received for 8 wk either a natural ingredient diet or a diet supplemented with 10 g/100 g linseed oil containing over 50% of 18:3 (n-3) alpha-linoleic acid. Other groups received PUFA containing only traces of 18:3 (n-3) fatty acid; beef tallow, containing mostly 18:1 (n-9) saturated fat, safflower oil, an 18:2 (n-6) PUFA, and fish oil, containing longer chain (n-3) PUFA. Each dietary group was divided into two subgroups: sedentary diet controls and exercised animals. Exercise consisted of continuous swimming at high intensity until exhaustion. It was shown in three separate experiments that (1) the primary humoral response to sheep red blood cells, determined by the plaque-forming cell (PFC) assay, was affected by PUFA diet in sedentary animals in the order beef tallow &amp;amp;gt; control diet &amp;amp;gt; safflower oil &amp;amp;gt; fish oil &amp;amp;gt; linseed oil, and (2) the PFC response was suppressed by the exhaustive exercise, as compared to sedentary controls, except for animals fed 18:3 (n-3) linseed oil, where the normal response was noted. Phagocytosis of fluorescent microspheres by peritoneal macrophages, determined by flow cytometry, was significantly lower in exercised animals receiving the linseed oil diet, whereas other diets either increased or did not significantly change the macrophage phagocytic activity, compared to the sedentary diet controls. Spleen lymphocyte subsets were unchanged in exercised animals except for a marked shift from the lymphoid peak toward the erythroid peak. Generally, our data showed a marked immunomodulatory effect of 18-3 (n-3) alpha-linoleic acid on the exhaustive exercise-related immunosuppression, as compared to the effects of other selected PUFA.
Eighteen male marathon runners (mean marathon performance: 2 h 36 min, SD = 7.0 min; VO2 max = 64... more Eighteen male marathon runners (mean marathon performance: 2 h 36 min, SD = 7.0 min; VO2 max = 64.1 +/- 15.1 ml/kg . min-1) were submitted to a needle biopsy in the suprailiac region and to various measurements of body fatness: percent body fat (% fat), seven skinfold thicknesses, and mean fat cell diameter. Basal and insulin-stimulated glucose conversion into triglycerides were measured in collagenase-isolated fat cells, while heparin-releasable lipoprotein lipase activity (LPLa) was determined in intact adipose tissue. All body fatness indicators were significantly smaller in marathon runners in comparison to a sedentary control group (P less than 0.001). Fat cell basal and insulin-stimulated glucose conversion into triglycerides as well as LPLa were significantly higher for the runners group (P less than 0.01), differences being particularly important when comparisons were performed between subjects paired for mean fat cell diameter. Pearson interclass correlations between body fatness and fat cell glucose incorporation into triglycerides were low and positive for the sedentary group (0.04 less than or equal to r less than or equal to 0.41), while they were negative for the marathon runners groups (-0.28 less than or equal to r less than or equal to -0.40) with the exception of % fat. Moreover, correlations between LPLa and indicators of body fatness were positive in the sedentary group (0.47 less than or equal to r less than or equal to 0.79), while they were negative in the marathon runners group (-0.03 less than or equal to r less than or equal to -0.63).(ABSTRACT TRUNCATED AT 250 WORDS)
Minerals, such as calcium and potassium, are essential for fetal development, but their transplac... more Minerals, such as calcium and potassium, are essential for fetal development, but their transplacental transport, and in particular, the effect of hormones on this process has not been extensively studied. Human alpha-calcitonin gene-related peptide (h alpha CGRP), a hormone constituted of 37 amino acids, is obtained by the alternative splicing of the mRNA from the calcitonin gene, and could be implicated in placental ion transport. In order to study the presence of this receptor, brush-border and basal plasma membranes were purified, and membrane binding studies were conducted using [125I]h alpha CGRP. The initiation of binding of [125I]h alpha CGRP to both membranes was rapid and reached maximal value after 10 min of incubation at 37 degrees C. Scratchard analysis revealed single-affinity binding sites for h alpha CGRP with Kd equal to 4412.45 +/- 604.81 pM and 2673.24 +/- 552.51 pM for brush-border and basal plasma membranes, respectively, which were significantly different. Moreover, the maximal number of receptors was significantly different (P &lt; 0.001) in both membranes, with Bmax of 627.94 +/- 31.40 fmol/mg protein for brush-border membranes and 343.70 +/- 43.52 fmol/mg protein in basal-plasma membranes. Competitive displacement of [125I]h alpha CGRP with other ligands showed the following potencies; h alpha CGRP approximately h beta CGRP approximately Cys (acm)2,7 CGRP &gt; CGRP (8-37), but no competition was observed with human and salmon calcitonin. Half-maximal displacement for human alpha- and beta CGRP was reached at approximately 10(-10)M for brush-border and basal-plasma membranes. alpha- and beta CGRP, and their fragment and analogue, stimulated cyclic AMP production in placental homogenate ranging from 143-163 per cent. Thus, our results show the presence of CGRP-specific receptors in both the syncytiotrophoblast membranes of human placenta. The role(s) of this related peptide in placenta remains to be investigated.
... Procedure for the determination of body density was as described by Behnke and Wilmore.&a... more ... Procedure for the determination of body density was as described by Behnke and Wilmore.&amp;quot; Percent fat was estimated from body density according to Siri.&amp;quot; The method of Wilmore et al&amp;#x27;8 was employed for the ... Collagenase was obtained from Worthington Laboratories. ...
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