Background: Though Diode laser irradiation has been used in various dental treatments, its harmfu... more Background: Though Diode laser irradiation has been used in various dental treatments, its harmful effects have not much been evaluated especially on human periodontal ligament cells. The aim of this study was to investigate diode laser irradiation effect on the viability and the proliferation of Human Periodontal Ligament Fibroblasts (HPLF). Materials and methods: HPLF cells were cultured in 96-well plates. The cultured cells were then irradiated with various power outputs (0.5W, 1W, 2W and 10W) of diode laser (810nm). The cell viability was determined by MTT assay. The cell proliferation activity of control and irradiated cells were evaluated for seven consecutive days. Results: The result showed comparative cell viability between test and control groups. At day 5, the test group showed significant higher cell proliferation than control group (p<0.05). The 2W group showed the highest cell viability and proliferation activity. Conclusion: The diode laser application in dentistr...
Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural prod... more Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural product. Previous research on chrysin's biological functions, including anticancer activity, had been reported. However, chrysin's effect on oral squamous cell carcinoma (OSCC) is still scarce. This article aimed to test the cytotoxicity, antiproliferative, antimigration, anti-invasion, and apoptotic effects of purified chrysin in two OSCC cell lines, HSC4 and SCC25. Materials and Methods The malignant phenotype was assessed using cell proliferation, wound healing, and transwell assays. Cell apoptosis was determined using flow cytometry. The positive control was OSCC cells treated with cisplatin, and the negative control was OSCC cells incubated with 0.1% dimethyl sulfoxide. Results Chrysin at concentrations of 100 and 200 µM could inhibit OSCC cell proliferation, migration, and invasion, as well as enhance cell apoptosis, particularly in the early stages of apoptosis. Conclusion In ...
The two clinically important classes of antimycotic drugs, the polyenes and azoles, act on the pl... more The two clinically important classes of antimycotic drugs, the polyenes and azoles, act on the plasma membrane of the cell. The primary modes of action are believed to be through interaction with sterols (polyenes) and alteration in sterol composition of the membrane (azoles). In this report we show that, at growth inhibitory concentrations, the polyenes (nystatin and amphotericin) and azoles (miconazole and ketoconazole) also inhibit plasma membrane enzymes. There was extensive (greater than 75%) inhibition of the Candida albicans plasma membrane enzymes ATPase, glucan synthase, adenyl cyclase and 5&#39;-nucleotidase, when assayed in situ. The antifungals papulacandin and echinocandin, which inhibit glucan synthesis, also inhibited plasma membrane enzymes in situ; glucan synthase (greater than 90%), 5&#39;-nucleotidase (greater than 80%) and ATPase (70-80%). Purified plasma membrane was prepared from yeast cells of C. albicans by two different techniques: concanavalin A stabilization and coating of spheroplasts with silica microbeads. In the purified plasma membrane vesicles prepared from concanavalin A the adenyl cyclase and phosphodiesterase were extensively (greater than 90%) inhibited by the three different classes of antifungal drugs; variable inhibition was observed with ATPase (70-100%). The 3&#39;,5&#39;-cyclic phosphodiesterase of the plasma membrane purified by the microbeads method was almost completely inhibited by all of the antifungals tested and there was partial inhibition of ATPase (20-85%) and adenyl cyclase (30-90%).
In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-h... more In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria Cupriavidus necator H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL). The water contact angle results indicated a hydrophobic character for all polymeric films. All fabricated scaffolds exhibited a high porosity of 90% with a sponge-like appearance. The P(HB-50HV) scaffolds were distinctively different in compressive modulus and was the material with the lowest stiffness among all scaffolds tested between the dry and wet conditions. The human gingival fibroblasts (HGFs) and periodontal ligament stem cells (PDLSCs) cultured onto the P(HB-50HV) scaffold adhered to the scaffold and exhibited the high...
Objectives This study aimed to evaluate the effect of Porphyromonas gingivalis and nicotine on th... more Objectives This study aimed to evaluate the effect of Porphyromonas gingivalis and nicotine on the in vitro osteogenic differentiation of periodontal ligament (PDL) fibroblasts. Materials and Methods PDLs were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum at 37°C under 5% CO2 and 100% humidified atmosphere. Cells were incubated with various concentrations of nicotine and P. gingivalis extracts, and cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. To study cell differentiation, PDLs (5 × 104cells) were treated with the osteogenic differentiation medium containing 10 mM β-glycerophosphate, 10 nM dexamethasone, 50 mg/mL ascorbic acid, 1 μM nicotine, and 50 µg/mL P. gingivalis lysate. mRNA samples were collected at 0, 7, and 14 days. Odontogenic-related gene expression, namely, Runt-related transcription factor 2 (Runx2), collagen type I (COL1A1), and alkaline phosphatase (ALP) was determined by reve...
Journal of the World Federation of Orthodontists, 2021
BACKGROUND The aim of this study was to investigate the influence of three different light-emitti... more BACKGROUND The aim of this study was to investigate the influence of three different light-emitting diode (LED) wavelengths on the proliferation and osteoblastic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. METHODS PDLSCs seeded on 96- and 24-well plates, for proliferation and osteoblastic differentiation, respectively, were irradiated daily by LED light with peak emission wavelengths of 630, 680, and 830 nm at constant energy densities of 3.5 J/cm2. Cultures were grown for 8 days for the proliferation assay, 10 days for the alkaline phosphatase (ALP) assay, and 28 days for Alizarin red staining. Mitochondrial activity, ALP enzyme level, and the ability to form calcium phosphate deposits were measured and compared across cultures. RESULTS Results obtained from statistical analysis of the experimental data indicated that the rate of proliferation (P < 0.05) in 830-nm irradiated cultures were significantly higher than the control samples at day 6 and 8; whereas, for the 630- and 680-nm groups, test results showed lower proliferation rates at day 8. For osteoblastic differentiation, significantly greater mineralization than the control samples was detected in the red-light groups (630 and 680 nm) during the late differentiation period (P < 0.001), which was supported by a higher ALP activity of the 630- and 680-nm groups in the early stage (P < 0.01). CONCLUSION The results of this study demonstrate that the PDLSCs responded differently to specific LED wavelengths. For enhancing cellular proliferation, 830-nm LED irradiation was more effective. On the other hand, the wavelengths of 630 and 680 nm were better for stimulating osteoblastic differentiation.
Abstract Background The effect of mechanical stimulation and photobiomodulation on tissue defect ... more Abstract Background The effect of mechanical stimulation and photobiomodulation on tissue defect restoration has recently been investigated in various fields of tissue engineering such as acceleration of healing process and bone calcification. Mechanical stimulation generates shear stress on bone cells resulting in promotion of bone formation whereas photobiomodulation regulates inflammation, decreases pain, accelerates cell proliferation and enhances healing. Methods MC3T3-E1 cells were cultured in 3 dimensional collagen scaffolds. Cells were daily stimulated by either mechanical loading of 3 Hz sinusoidal with 3000 μstrain vibration, or photobiomodulation using LED with 3 J/cm 2 fluency or combination of both. The calcifications of 3D tissue-engineered bones were examined by non-destructive monitoring device every day for 42 days. Results The 3D tissue-engineered bones that exposed to mechanical alone or combined stimulation exhibited early calcification, higher calcium content and bulk density comparing to control and light stimulation alone. Furthermore, the mRNA expression level of bone formation related genes such as RUNX2, ALP, osteopontin and osteocalcin were examined 7 days after stimulations. We showed the potential upregulation of ALP gene after mechanical stimulation alone or combined with light treatment. On day 28 Von Kossa stain revealed higher calcium deposition and increased cell migration to the deeper zone of 3D tissue-engineered bones. Conclusion We suggested that the mechanical treatment alone and combination with light treatment could accelerate the calcification of 3D tissue-engineered bone possibly through up-regulation of ALP gene during early stage of bone formation.
Background: Though Diode laser irradiation has been used in various dental treatments, its harmfu... more Background: Though Diode laser irradiation has been used in various dental treatments, its harmful effects have not much been evaluated especially on human periodontal ligament cells. The aim of this study was to investigate diode laser irradiation effect on the viability and the proliferation of Human Periodontal Ligament Fibroblasts (HPLF). Materials and methods: HPLF cells were cultured in 96-well plates. The cultured cells were then irradiated with various power outputs (0.5W, 1W, 2W and 10W) of diode laser (810nm). The cell viability was determined by MTT assay. The cell proliferation activity of control and irradiated cells were evaluated for seven consecutive days. Results: The result showed comparative cell viability between test and control groups. At day 5, the test group showed significant higher cell proliferation than control group (p<0.05). The 2W group showed the highest cell viability and proliferation activity. Conclusion: The diode laser application in dentistr...
Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural prod... more Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural product. Previous research on chrysin's biological functions, including anticancer activity, had been reported. However, chrysin's effect on oral squamous cell carcinoma (OSCC) is still scarce. This article aimed to test the cytotoxicity, antiproliferative, antimigration, anti-invasion, and apoptotic effects of purified chrysin in two OSCC cell lines, HSC4 and SCC25. Materials and Methods The malignant phenotype was assessed using cell proliferation, wound healing, and transwell assays. Cell apoptosis was determined using flow cytometry. The positive control was OSCC cells treated with cisplatin, and the negative control was OSCC cells incubated with 0.1% dimethyl sulfoxide. Results Chrysin at concentrations of 100 and 200 µM could inhibit OSCC cell proliferation, migration, and invasion, as well as enhance cell apoptosis, particularly in the early stages of apoptosis. Conclusion In ...
The two clinically important classes of antimycotic drugs, the polyenes and azoles, act on the pl... more The two clinically important classes of antimycotic drugs, the polyenes and azoles, act on the plasma membrane of the cell. The primary modes of action are believed to be through interaction with sterols (polyenes) and alteration in sterol composition of the membrane (azoles). In this report we show that, at growth inhibitory concentrations, the polyenes (nystatin and amphotericin) and azoles (miconazole and ketoconazole) also inhibit plasma membrane enzymes. There was extensive (greater than 75%) inhibition of the Candida albicans plasma membrane enzymes ATPase, glucan synthase, adenyl cyclase and 5&#39;-nucleotidase, when assayed in situ. The antifungals papulacandin and echinocandin, which inhibit glucan synthesis, also inhibited plasma membrane enzymes in situ; glucan synthase (greater than 90%), 5&#39;-nucleotidase (greater than 80%) and ATPase (70-80%). Purified plasma membrane was prepared from yeast cells of C. albicans by two different techniques: concanavalin A stabilization and coating of spheroplasts with silica microbeads. In the purified plasma membrane vesicles prepared from concanavalin A the adenyl cyclase and phosphodiesterase were extensively (greater than 90%) inhibited by the three different classes of antifungal drugs; variable inhibition was observed with ATPase (70-100%). The 3&#39;,5&#39;-cyclic phosphodiesterase of the plasma membrane purified by the microbeads method was almost completely inhibited by all of the antifungals tested and there was partial inhibition of ATPase (20-85%) and adenyl cyclase (30-90%).
In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-h... more In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria Cupriavidus necator H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL). The water contact angle results indicated a hydrophobic character for all polymeric films. All fabricated scaffolds exhibited a high porosity of 90% with a sponge-like appearance. The P(HB-50HV) scaffolds were distinctively different in compressive modulus and was the material with the lowest stiffness among all scaffolds tested between the dry and wet conditions. The human gingival fibroblasts (HGFs) and periodontal ligament stem cells (PDLSCs) cultured onto the P(HB-50HV) scaffold adhered to the scaffold and exhibited the high...
Objectives This study aimed to evaluate the effect of Porphyromonas gingivalis and nicotine on th... more Objectives This study aimed to evaluate the effect of Porphyromonas gingivalis and nicotine on the in vitro osteogenic differentiation of periodontal ligament (PDL) fibroblasts. Materials and Methods PDLs were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum at 37°C under 5% CO2 and 100% humidified atmosphere. Cells were incubated with various concentrations of nicotine and P. gingivalis extracts, and cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. To study cell differentiation, PDLs (5 × 104cells) were treated with the osteogenic differentiation medium containing 10 mM β-glycerophosphate, 10 nM dexamethasone, 50 mg/mL ascorbic acid, 1 μM nicotine, and 50 µg/mL P. gingivalis lysate. mRNA samples were collected at 0, 7, and 14 days. Odontogenic-related gene expression, namely, Runt-related transcription factor 2 (Runx2), collagen type I (COL1A1), and alkaline phosphatase (ALP) was determined by reve...
Journal of the World Federation of Orthodontists, 2021
BACKGROUND The aim of this study was to investigate the influence of three different light-emitti... more BACKGROUND The aim of this study was to investigate the influence of three different light-emitting diode (LED) wavelengths on the proliferation and osteoblastic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. METHODS PDLSCs seeded on 96- and 24-well plates, for proliferation and osteoblastic differentiation, respectively, were irradiated daily by LED light with peak emission wavelengths of 630, 680, and 830 nm at constant energy densities of 3.5 J/cm2. Cultures were grown for 8 days for the proliferation assay, 10 days for the alkaline phosphatase (ALP) assay, and 28 days for Alizarin red staining. Mitochondrial activity, ALP enzyme level, and the ability to form calcium phosphate deposits were measured and compared across cultures. RESULTS Results obtained from statistical analysis of the experimental data indicated that the rate of proliferation (P < 0.05) in 830-nm irradiated cultures were significantly higher than the control samples at day 6 and 8; whereas, for the 630- and 680-nm groups, test results showed lower proliferation rates at day 8. For osteoblastic differentiation, significantly greater mineralization than the control samples was detected in the red-light groups (630 and 680 nm) during the late differentiation period (P < 0.001), which was supported by a higher ALP activity of the 630- and 680-nm groups in the early stage (P < 0.01). CONCLUSION The results of this study demonstrate that the PDLSCs responded differently to specific LED wavelengths. For enhancing cellular proliferation, 830-nm LED irradiation was more effective. On the other hand, the wavelengths of 630 and 680 nm were better for stimulating osteoblastic differentiation.
Abstract Background The effect of mechanical stimulation and photobiomodulation on tissue defect ... more Abstract Background The effect of mechanical stimulation and photobiomodulation on tissue defect restoration has recently been investigated in various fields of tissue engineering such as acceleration of healing process and bone calcification. Mechanical stimulation generates shear stress on bone cells resulting in promotion of bone formation whereas photobiomodulation regulates inflammation, decreases pain, accelerates cell proliferation and enhances healing. Methods MC3T3-E1 cells were cultured in 3 dimensional collagen scaffolds. Cells were daily stimulated by either mechanical loading of 3 Hz sinusoidal with 3000 μstrain vibration, or photobiomodulation using LED with 3 J/cm 2 fluency or combination of both. The calcifications of 3D tissue-engineered bones were examined by non-destructive monitoring device every day for 42 days. Results The 3D tissue-engineered bones that exposed to mechanical alone or combined stimulation exhibited early calcification, higher calcium content and bulk density comparing to control and light stimulation alone. Furthermore, the mRNA expression level of bone formation related genes such as RUNX2, ALP, osteopontin and osteocalcin were examined 7 days after stimulations. We showed the potential upregulation of ALP gene after mechanical stimulation alone or combined with light treatment. On day 28 Von Kossa stain revealed higher calcium deposition and increased cell migration to the deeper zone of 3D tissue-engineered bones. Conclusion We suggested that the mechanical treatment alone and combination with light treatment could accelerate the calcification of 3D tissue-engineered bone possibly through up-regulation of ALP gene during early stage of bone formation.
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Papers by Rudee Surarit