Objectives Juvenile obesity is a complex clinical condition that is present more and more frequen... more Objectives Juvenile obesity is a complex clinical condition that is present more and more frequently in the daily orthodontic practice. Over-weighted patients have an impaired bone metabolism, due in part to their increased levels of circulating adipokines. Particularly, leptin has been reported to play a key role in bone physiology. Leptin is ubiquitously present in the body, including blood, saliva, and crevicular fluid. If, and to what extent, it could influence the reaction of cementoblasts during orthodontic-induced forces is yet unknown. Material and methods OCCM-30 cementoblasts were cultivated under compressive forces using different concentrations of leptin. The expression of ObR, Runx-2, Osteocalcin, Rank-L, Sost, Caspase 3, 8, and 9 were analyzed by RT-PCR. Western blots were employed for protein analysis. The ERK1/2 antagonist FR180204 (Calbiochem) was used and cPLA2 activation, PGE2, and cytochrome C release were further evaluated. Results In vitro, when compressive for...
This prospective Herbst study analyzed the sagittal dental and skeletal changes contributing to C... more This prospective Herbst study analyzed the sagittal dental and skeletal changes contributing to Class II correction in young adults. Additionally, the alteration in skeletal and soft tissue convexity occurring during treatment was assessed. Early adolescent subjects in the ...
Objectives Juvenile obesity is a complex clinical condition that is present more and more frequen... more Objectives Juvenile obesity is a complex clinical condition that is present more and more frequently in the daily orthodontic practice. Over-weighted patients have an impaired bone metabolism, due in part to their increased levels of circulating adipokines. Particularly, leptin has been reported to play a key role in bone physiology. Leptin is ubiquitously present in the body, including blood, saliva, and crevicular fluid. If, and to what extent, it could influence the reaction of cementoblasts during orthodontic-induced forces is yet unknown. Material and methods OCCM-30 cementoblasts were cultivated under compressive forces using different concentrations of leptin. The expression of ObR, Runx-2, Osteocalcin, Rank-L, Sost, Caspase 3, 8, and 9 were analyzed by RT-PCR. Western blots were employed for protein analysis. The ERK1/2 antagonist FR180204 (Calbiochem) was used and cPLA2 activation, PGE2, and cytochrome C release were further evaluated. Results In vitro, when compressive for...
This prospective Herbst study analyzed the sagittal dental and skeletal changes contributing to C... more This prospective Herbst study analyzed the sagittal dental and skeletal changes contributing to Class II correction in young adults. Additionally, the alteration in skeletal and soft tissue convexity occurring during treatment was assessed. Early adolescent subjects in the ...
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