Heat shock protein (HSP70) was used as a good biomarker for intoxication of different hazardous c... more Heat shock protein (HSP70) was used as a good biomarker for intoxication of different hazardous chemicals. The impact of common narcotic drugs (heroin and opium), in comparison with experimentally proven hepatotoxic agents (CCl 4 , ethanol and iron) on the induction of HSP70 in liver of both rat and mice was assessed. Heroin proved to be the most potent inducer of liver tissue intoxication followed by CCl 4 , iron overloaded with ferric hydroxide dextran complex (Fe-HDC), ethanol, and opium. This may be related to defense mechanism against the rapidly occurring cell damage or subsequent processes of liver tissue inflammation. This is an attempt to define the incidence and severity of liver disorders among a large number of drug addicts. The expression levels of HSP70 were measured using Western dot blot technique. The obtained data demonstrate specific but statistically different significant values (P< 0.05) of HSP70 induction levels with all studied hepatotoxic agents. Heroin wa...
Abstract: The endophytic fungus Penicillium citrinum was isolated from fresh stem tissues of the ... more Abstract: The endophytic fungus Penicillium citrinum was isolated from fresh stem tissues of the Ceratonia siliqua plant. Extracts of P. citrinum grown on white-bean medium yielded four new compounds, namely tanzawaic acids G and H, and 3,5-dimethyl-6-hydroxy-8-methoxyisoquinoline, and the new natural product, 1,2,3,11b-tetrahydroquinolactacide that had hitherto been only described as a synthetic compound. In addition, nine known compounds including five alkaloids and four polyketides were isolated. The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. Penicinoline and 3,6-dibenzyl-2,5 piperazinedione showed moderate cytotoxic activity against the murine lymphoma L5178Y cell line using MTT assay, while the remaining isolated compounds are cytotoxic inactive in this assay.
Heat shock protein (HSP70) was used as a good biomarker for intoxication of different hazardous c... more Heat shock protein (HSP70) was used as a good biomarker for intoxication of different hazardous chemicals. The impact of common narcotic drugs (heroin and opium), in comparison with experimentally proven hepatotoxic agents (CCl 4 , ethanol and iron) on the induction of HSP70 in liver of both rat and mice was assessed. Heroin proved to be the most potent inducer of liver tissue intoxication followed by CCl 4 , iron overloaded with ferric hydroxide dextran complex (Fe-HDC), ethanol, and opium. This may be related to defense mechanism against the rapidly occurring cell damage or subsequent processes of liver tissue inflammation. This is an attempt to define the incidence and severity of liver disorders among a large number of drug addicts. The expression levels of HSP70 were measured using Western dot blot technique. The obtained data demonstrate specific but statistically different significant values (P< 0.05) of HSP70 induction levels with all studied hepatotoxic agents. Heroin wa...
Abstract: The endophytic fungus Penicillium citrinum was isolated from fresh stem tissues of the ... more Abstract: The endophytic fungus Penicillium citrinum was isolated from fresh stem tissues of the Ceratonia siliqua plant. Extracts of P. citrinum grown on white-bean medium yielded four new compounds, namely tanzawaic acids G and H, and 3,5-dimethyl-6-hydroxy-8-methoxyisoquinoline, and the new natural product, 1,2,3,11b-tetrahydroquinolactacide that had hitherto been only described as a synthetic compound. In addition, nine known compounds including five alkaloids and four polyketides were isolated. The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. Penicinoline and 3,6-dibenzyl-2,5 piperazinedione showed moderate cytotoxic activity against the murine lymphoma L5178Y cell line using MTT assay, while the remaining isolated compounds are cytotoxic inactive in this assay.
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