Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan,... more Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan, Mazandaran and Golestan provinces in the north of Iran) was estimated using four microsatellite loci. For this purpose, DNA was extracted from the thoracic part of 100 worker honey bees from each province, according to the salting out method. Following amplification, products electrophoresed on 6% denaturing polyacrylamide gel. The results indicated that, the honey bees in the north of Iran had a low variability in terms of number of alleles per locus and heterozygosity. The results of this research confirm the previous morphological and biochemical indications of uniformity in the honey bee population of the south strip of the Caspian Sea despite a geographical spread of 1,000 km.
Az albumin es transzferrin polimorfizmusat vizsgaltak a kovetkező 5 irani helyi tyuk fajtaiban, m... more Az albumin es transzferrin polimorfizmusat vizsgaltak a kovetkező 5 irani helyi tyuk fajtaiban, melyet genetikai tartalekkent tenyesztettek: kopasznyaku, Dashtyary, Lary, Marandy es parlagi. Az albumin A frekvenciaja rendkivul alacsony vagy az allei egyaltalan nincs is jelen a vizsgalt allomanyokban (0.000-0.031), a B allel nagyon gyakori (0.917-0.960), a C frekvenciaja pedig 0.020-0.016 kozott van. A transzferrin A allel frekvenciaja 0.016-0.075, a B-e 0.355-0.523, mig a C-e 0.522-0.629 a vizsgalt fajtakban. A transzferrin heterozigozitas indexe az ertekelt fajtakra vonatkoztatva 0.478-0.559, az albumine pedig 0.077-0.155 kozott valtozott. A veletlenszerűen kivalasztott mintakban a csirkek megkulonboztethetősegenek pontossaga az albumin eseteben rendkivul alacsony (PD0.71).
(1) Genetic distances and heterozygosity were determined in 5 Iranian native chickens: Dashtyary,... more (1) Genetic distances and heterozygosity were determined in 5 Iranian native chickens: Dashtyary, Lary, Marandy, Naked neck and Common breed, using three blood group systems (A, B and D) and 4 serum protein loci (albumin, transferrin, alkaline phosphatase and esterase). (2) The highest D(A) and D(S) were obtained between Dashtyary and Lary breeds. (3) The average heterozygosity for each breed in all loci ranged from 0.330 for Dashtyary to 0.370 for Common breed. (4) Dendrograms based on an unweighted pair-group method using an arithmetic average (UPGMA) showed two distinct clusters. One cluster included Dashtyary and the other contained the remaining 4 breeds.
5 helyi irani genetikai tartalekkent tenyesztett kopasznyaku, Dashtyary, Lary, Marandy es parlagi... more 5 helyi irani genetikai tartalekkent tenyesztett kopasznyaku, Dashtyary, Lary, Marandy es parlagi tyukfajta vermintait gyűjtottek ossze. 3 vercsoport rendszert es 5 allei frekvenciat irtak le (A2, A6, B4, B7 es D4). A B7-es allei fordult elő a vizsgalt fajtakban leggyakrabban (0.24-0.31 kozott), az A2 gyakorisaga 0.14-0.33, az A6-e 0.14-0.26, a B4-e 0.18-0.24, a D4-e pedig 0.08-0.16. A vercsoport allelekre alapozva az irani fajtak heterozigozitas indexe magas (0.77-0.80) volt a nepali es bangladesi helyi fajtakhoz viszonyitva. Annak lehetősege es pontossaga, hogy egy fajtabol veletlenszerűen kivalasztott csirkek megkulonboztethetőek legyenek egymastol a vercsoport allelek alapjan PD>0.90.
Twenty four Holstein calves at birth (initial BW= 40±3.0 kg) were used to study the effects of ma... more Twenty four Holstein calves at birth (initial BW= 40±3.0 kg) were used to study the effects of mannanoligosaccharide–β Glucan (MOS-β Glucan) or antibiotics on health and performance of dairy calves. Calves were assigned randomly to one of three treatments. Treatments included: whole milk with no additives (control), whole milk containing MOS-β Glucan at 4g per meal, whole milk containing antibiotic at 500 mg of oxytetracycline/d. Calves received whole milk twice daily. Water and starter were offered ad lib throughout the trial of 56 days. Body weight were measured at birth and thereafter weekly till 8 wk of age. Starter intake were measured daily. Faecal scores were monitored 3 times per week. Blood samples were collected at 3, 7, 15, 30, 45 and 56 days, and analyzed for total protein, blood urea N, albumin and ratio albumin:glubolin. Analysis of weekly DMI revealed no significant difference from week 1 to 7 among the treatment. The calves fed MOS-β Glucan or antibiotic at 8 week ha...
This study investigated the effects of supplementing Lake extender with cysteamine (CYS) on roost... more This study investigated the effects of supplementing Lake extender with cysteamine (CYS) on rooster semen quality in cold storage and it's fertility performance. Semen samples were diluted with Lake extender supplemented with different concentrations of CYS (0, 1, 2, 4 and 8 mM) and were cooled and stored at 5 °C for a period of 46 h. Motility, membrane functionality, viability, lipid peroxidation, and mitochondria membrane potential were evaluated at 0, 23 and 46 h of storage. Fertility was assessed at 23 h of storage. Although at the beginning time (0 h), parameters were not affected, 1 mM of CYS improved (P ≤ 0.05) total motility, progressive motility and mitochondria membrane potential during 23 and 46 h storage. Moreover, 1 and 2 mM CYS improved (P ≤ 0.05) membrane functionality and viability compared to other groups. Lipid peroxidation was lower (P ≤ 0.05) in samples diluted with 1 and 2 mM CYS compared to the others. Artificial insemination with 23-hrs cooled-stored semen produced the higher (P ≤ 0.05) fertility rate in groups received 1 and 2 mM CYS compared to the control group. In conclusion, addition of 1 and 2 mM CYS to the extender could be helpful to protect rooster semen against structural and functional damages of cooling storage process.
Sperm cryopreservation is used to spread qualified semen for artificial insemination, but the fre... more Sperm cryopreservation is used to spread qualified semen for artificial insemination, but the freezing process reduces sperm quality. This study assessed the efficacy of Mito-TEMPO on post-thawed goat sperm quality. Semen samples divided to five equal groups and after dilution, received different doses of Mito-TEMPO (0, 1, 10, 100 and 1000 μM), and cryopreserved in liquid nitrogen. After thawing, flow cytometry analysis was performed to evaluate sperm mitochondria membrane potential, viability, apoptotic-like changes, DNA fragmentation and ROS concentration. According to the results, Mito-TEMPO (10 and 100 μM) improved (P≤0.05) sperm viability and decreased (P≤0.05) apoptotic-like changes and ROS concentration compared to the other groups. Mitochondria membrane potential was higher (P≤0.05) in groups received 1, 10 and 100 μM Mito-TEMPO. The lowest (P≤0.05) DNA fragmentation was observed in group received 10 μM Mito-TEMPO. In conclusion, mitochondria-targeted antioxidant Mito-TEMPO could be an efficient cryo-additive to enhance flowcytometric quality parameters of post-thawed bulk semen.
Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan,... more Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan, Mazandaran and Golestan provinces in the north of Iran) was estimated using four microsatellite loci. For this purpose, DNA was extracted from the thoracic part of 100 worker honey bees from each province, according to the salting out method. Following amplification, products electrophoresed on 6% denaturing polyacrylamide gel. The results indicated that, the honey bees in the north of Iran had a low variability in terms of number of alleles per locus and heterozygosity. The results of this research confirm the previous morphological and biochemical indications of uniformity in the honey bee population of the south strip of the Caspian Sea despite a geographical spread of 1,000 km.
Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan,... more Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan, Mazandaran and Golestan provinces in the north of Iran) was estimated using four microsatellite loci. For this purpose, DNA was extracted from the thoracic part of 100 worker honey bees from each province, according to the salting out method. Following amplification, products electrophoresed on 6% denaturing polyacrylamide gel. The results indicated that, the honey bees in the north of Iran had a low variability in terms of number of alleles per locus and heterozygosity. The results of this research confirm the previous morphological and biochemical indications of uniformity in the honey bee population of the south strip of the Caspian Sea despite a geographical spread of 1,000 km.
Az albumin es transzferrin polimorfizmusat vizsgaltak a kovetkező 5 irani helyi tyuk fajtaiban, m... more Az albumin es transzferrin polimorfizmusat vizsgaltak a kovetkező 5 irani helyi tyuk fajtaiban, melyet genetikai tartalekkent tenyesztettek: kopasznyaku, Dashtyary, Lary, Marandy es parlagi. Az albumin A frekvenciaja rendkivul alacsony vagy az allei egyaltalan nincs is jelen a vizsgalt allomanyokban (0.000-0.031), a B allel nagyon gyakori (0.917-0.960), a C frekvenciaja pedig 0.020-0.016 kozott van. A transzferrin A allel frekvenciaja 0.016-0.075, a B-e 0.355-0.523, mig a C-e 0.522-0.629 a vizsgalt fajtakban. A transzferrin heterozigozitas indexe az ertekelt fajtakra vonatkoztatva 0.478-0.559, az albumine pedig 0.077-0.155 kozott valtozott. A veletlenszerűen kivalasztott mintakban a csirkek megkulonboztethetősegenek pontossaga az albumin eseteben rendkivul alacsony (PD0.71).
(1) Genetic distances and heterozygosity were determined in 5 Iranian native chickens: Dashtyary,... more (1) Genetic distances and heterozygosity were determined in 5 Iranian native chickens: Dashtyary, Lary, Marandy, Naked neck and Common breed, using three blood group systems (A, B and D) and 4 serum protein loci (albumin, transferrin, alkaline phosphatase and esterase). (2) The highest D(A) and D(S) were obtained between Dashtyary and Lary breeds. (3) The average heterozygosity for each breed in all loci ranged from 0.330 for Dashtyary to 0.370 for Common breed. (4) Dendrograms based on an unweighted pair-group method using an arithmetic average (UPGMA) showed two distinct clusters. One cluster included Dashtyary and the other contained the remaining 4 breeds.
5 helyi irani genetikai tartalekkent tenyesztett kopasznyaku, Dashtyary, Lary, Marandy es parlagi... more 5 helyi irani genetikai tartalekkent tenyesztett kopasznyaku, Dashtyary, Lary, Marandy es parlagi tyukfajta vermintait gyűjtottek ossze. 3 vercsoport rendszert es 5 allei frekvenciat irtak le (A2, A6, B4, B7 es D4). A B7-es allei fordult elő a vizsgalt fajtakban leggyakrabban (0.24-0.31 kozott), az A2 gyakorisaga 0.14-0.33, az A6-e 0.14-0.26, a B4-e 0.18-0.24, a D4-e pedig 0.08-0.16. A vercsoport allelekre alapozva az irani fajtak heterozigozitas indexe magas (0.77-0.80) volt a nepali es bangladesi helyi fajtakhoz viszonyitva. Annak lehetősege es pontossaga, hogy egy fajtabol veletlenszerűen kivalasztott csirkek megkulonboztethetőek legyenek egymastol a vercsoport allelek alapjan PD>0.90.
Twenty four Holstein calves at birth (initial BW= 40±3.0 kg) were used to study the effects of ma... more Twenty four Holstein calves at birth (initial BW= 40±3.0 kg) were used to study the effects of mannanoligosaccharide–β Glucan (MOS-β Glucan) or antibiotics on health and performance of dairy calves. Calves were assigned randomly to one of three treatments. Treatments included: whole milk with no additives (control), whole milk containing MOS-β Glucan at 4g per meal, whole milk containing antibiotic at 500 mg of oxytetracycline/d. Calves received whole milk twice daily. Water and starter were offered ad lib throughout the trial of 56 days. Body weight were measured at birth and thereafter weekly till 8 wk of age. Starter intake were measured daily. Faecal scores were monitored 3 times per week. Blood samples were collected at 3, 7, 15, 30, 45 and 56 days, and analyzed for total protein, blood urea N, albumin and ratio albumin:glubolin. Analysis of weekly DMI revealed no significant difference from week 1 to 7 among the treatment. The calves fed MOS-β Glucan or antibiotic at 8 week ha...
This study investigated the effects of supplementing Lake extender with cysteamine (CYS) on roost... more This study investigated the effects of supplementing Lake extender with cysteamine (CYS) on rooster semen quality in cold storage and it's fertility performance. Semen samples were diluted with Lake extender supplemented with different concentrations of CYS (0, 1, 2, 4 and 8 mM) and were cooled and stored at 5 °C for a period of 46 h. Motility, membrane functionality, viability, lipid peroxidation, and mitochondria membrane potential were evaluated at 0, 23 and 46 h of storage. Fertility was assessed at 23 h of storage. Although at the beginning time (0 h), parameters were not affected, 1 mM of CYS improved (P ≤ 0.05) total motility, progressive motility and mitochondria membrane potential during 23 and 46 h storage. Moreover, 1 and 2 mM CYS improved (P ≤ 0.05) membrane functionality and viability compared to other groups. Lipid peroxidation was lower (P ≤ 0.05) in samples diluted with 1 and 2 mM CYS compared to the others. Artificial insemination with 23-hrs cooled-stored semen produced the higher (P ≤ 0.05) fertility rate in groups received 1 and 2 mM CYS compared to the control group. In conclusion, addition of 1 and 2 mM CYS to the extender could be helpful to protect rooster semen against structural and functional damages of cooling storage process.
Sperm cryopreservation is used to spread qualified semen for artificial insemination, but the fre... more Sperm cryopreservation is used to spread qualified semen for artificial insemination, but the freezing process reduces sperm quality. This study assessed the efficacy of Mito-TEMPO on post-thawed goat sperm quality. Semen samples divided to five equal groups and after dilution, received different doses of Mito-TEMPO (0, 1, 10, 100 and 1000 μM), and cryopreserved in liquid nitrogen. After thawing, flow cytometry analysis was performed to evaluate sperm mitochondria membrane potential, viability, apoptotic-like changes, DNA fragmentation and ROS concentration. According to the results, Mito-TEMPO (10 and 100 μM) improved (P≤0.05) sperm viability and decreased (P≤0.05) apoptotic-like changes and ROS concentration compared to the other groups. Mitochondria membrane potential was higher (P≤0.05) in groups received 1, 10 and 100 μM Mito-TEMPO. The lowest (P≤0.05) DNA fragmentation was observed in group received 10 μM Mito-TEMPO. In conclusion, mitochondria-targeted antioxidant Mito-TEMPO could be an efficient cryo-additive to enhance flowcytometric quality parameters of post-thawed bulk semen.
Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan,... more Summary The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan, Mazandaran and Golestan provinces in the north of Iran) was estimated using four microsatellite loci. For this purpose, DNA was extracted from the thoracic part of 100 worker honey bees from each province, according to the salting out method. Following amplification, products electrophoresed on 6% denaturing polyacrylamide gel. The results indicated that, the honey bees in the north of Iran had a low variability in terms of number of alleles per locus and heterozygosity. The results of this research confirm the previous morphological and biochemical indications of uniformity in the honey bee population of the south strip of the Caspian Sea despite a geographical spread of 1,000 km.
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