Three new hydrolyzable tannins, cocciferins D(1) (1), D(2) (2), and T(1) (4), were isolated from ... more Three new hydrolyzable tannins, cocciferins D(1) (1), D(2) (2), and T(1) (4), were isolated from the leaves of Quercus coccifera. Cocciferin D(2) (2) and two additional new tannins, cocciferins D(3) (3) and T(2) (5), were also obtained from the leaves of Quercus suber. Their oligomeric structures were elucidated on the basis of spectroscopic methods and chemical evidence. Compounds 2, 3, and 5 were rare oligomers possessing glucose cores with both open-chain and pyranose forms.
The present study is designed to evaluate the antioxidant and anti-inflammatory effects of Parony... more The present study is designed to evaluate the antioxidant and anti-inflammatory effects of Paronychia argentea methanolic extract against acetic acid-induced ulcerative colitis in mice. The experimental colitis in mice was induced with 5% acetic acid (v/v, in saline) via rectal route. Animals were then divided into various treatment groups (n = 12). Four groups were treated with P. argentea methanolic extract (PAME) (50, 100, 200 and 500 mg/kg) for 5 consecutive days, and one group was treated with sulfasalazine (SASP, 500 mg/kg) as a standard drug. Disease activity index (DAI), colon weight and length, hematological parameters (C-reactive protein: CRP, erythrocyte sedimentation rate: ESR and full blood count: FBC), colonic contents of Malondialdehyde (MDA) and reduced glutathione (GSH), colonic superoxide dismutase (SOD) and catalase (CAT) activities were recorded. Results revealed that the intrarectal instillation of acetic acid caused increased DAI, colon weight, CRP, ESR and colonic MDA levels, and a decrease in the other parameters such as colon length, FBC, colonic GSH levels, SOD and CAT activities. Treatment with PAME showed significant effect in lowering DAI, colon weight, CRP, ESR and colonic MDA levels, and in enhancing the colon length, FBC, colonic GSH levels, SOD and CAT activities in a dose-dependent manner. These findings suggest that the extract obtained from P. argentea possess active substances, which exert marked protective effects in acute experimental colitis by regulating inflammatory markers and antioxidant parameters. Paronychia argentea may serve as a natural antioxidant and anti-inflammatory therapy for ulcerative colitis.
Biomolecules are largely found at different parts in divers medicinal plants. This study aims to ... more Biomolecules are largely found at different parts in divers medicinal plants. This study aims to quantify polyphenols and some biological properties of the areal part of Rubus idaeus L. plant. The shoots of Rubus idaeus L. were extracted by a methanol-water mixture. The antioxidant capacity was estimated by different in vitro assays ; linoleic acid emulsion model system, ß-carotene-linoleate model system, DPPH reduction, free radical-scavenging activity, and reducing ability assay. The highest levels of polyphenolic compounds was observed in the ethyl acetate extract (EAE). In linoleic acid test, chloroform extract (CHE) had the strongest antioxidant in comparison with butylated hydroxyl toluene (BHT) (64. 549 ± 0. 007%). In b-carotene/linoleic acid bleaching assay, the crude extract (CE) had the highest antioxidant capacity. The results showed that EAE showed the strongest antioxidant activity as measured by radicals scavenging activities and reducing power.The present work provide...
ETHNOPHARMACOLOGICAL RELEVANCE Erica arborea known as Khlenj in Algeria is a small shrub belongin... more ETHNOPHARMACOLOGICAL RELEVANCE Erica arborea known as Khlenj in Algeria is a small shrub belonging to Ericaceae family. E. arborea Aqueous extract (EAAE) is used in traditional medicine for anti-inflammatory, diuretic, antimicrobial, and antiulcer purposes. AIM OF THE STUDY To our knowledge, no data reveal the combination between in-vivo anti-inflammatory and toxicological studies of EAAE. For this purpose, the aim of this study is to evaluate the biological activity cited above and asses its safety. MATERIAL AND METHODS Anti-inflammatory activity was undergone using carrageenan-induced paw edema and croton oil-induced ear edema. The acute and sub-acute toxicity were conducted following the OECD guidelines 423 and 407 respectively. Phytochemical identification was carried out using HPLC-DAD-MS. Quantitative evaluation of polyphenols; flavonoids and antioxidant activity of EAAE were also determined. RESULTS Oral administration of EAAE (250 and 500 mg/kg) significantly (p <0.05) reduced the edema induced by carrageenan. Administration of EAAE dosed at 250 and 500 mg/kg exhibited efficacy in reducing edema induced by croton oil. The acute administration of EAAE at doses of 2000 and 5000 mg/kg did not cause any mortality or adverse effects indicating that the LD50 is above 5000 mg/kg. The prolonged administration of EAAE (500 and 1000 mg/kg) showed a significant reduction in triglycerides levels in male and female rats whereas no significant changes in other biochemical and hematological parameters were observed. Histopathological damages were recorded in both liver and kidney animal's tissues of both sexes treated with medium and maximum doses of EAAE. Phytochemical characterization of EAAE revealed a high amount of phenolic compounds, HPLC-DAD-MS analysis led to the identification of chlorogenic acid and five flavonol glycosides: myricetin pentoside, quercetin 3-O-glucoside, myricetin 3-O-rhamnoside, quercetin-3-O-pentoside, and quercetin-3-O-rhamnoside. CONCLUSION In the light of the results obtained in this study, EAAE corroborates the popular use to treat the anti-inflammatory impairments. EAAE can be considered as non-toxic in acute administration and exhibited a moderate toxicity in sub-acute administration. High phenolic content and in-vitro antioxidant activity observed indicate that EAAE may reduce oxidative stress markers in-vivo.
Centaurea species are widely used in traditional medicine to treat several illnesses, especially ... more Centaurea species are widely used in traditional medicine to treat several illnesses, especially by Mediterranean populations due to their pharmacological properties. The present study aimed to evaluate for the first time some in vivo activities of n-butanol (n-BuOH) extract of the aerial part of Centaurea tougourensis. For this approach; the antidiabetic (streptozotocin-induced diabetes), hepatoprotective (paracetamol induced hepatotoxicity), anti-inflammatory (croton oil induced ear edema assay) and antipyretic activities of this plant extract were tested. The pharmacological results suggest that C. tougourensis has a non-negligible anti-inflammatory effect on the formation of ear edema with a maximum inhibition percentage of (39.58%) for the highest tested concentration of 400 mg/kg. However, the antipyretic activity of the plant was remarkable for both tested concentrations (200 and 400 mg/kg) 5 h after treatment with a significant (P < 0.05) reduction of rectal temperature to (32.88 ± 0.23°C) and (32.36 ± 0.18°C) which correspond to a pyrexia inhibition of (78.9%) and (90.18%) respectively. C. tougourensis exhibited also a good anti-hyperglycemic effect which reached an inhibition percentage of (68.29%) at the end of the 3rd week of treatment for the tested concentration of 400 mg/kg and was considered almost similar to those of standard value (71.83%) at the same time. The n-BuOH extract C. tougourensis showed also a remarkable hepatoprotective effect which was confirmed by biochemical and histological approaches of note is that natural silymarin was also used as reference drug and showed a remarkable hepatoprotective effect. These encouraging results demonstrated once again the pharmacological potential of Centaurea species.
Background: Species of Echium trygorrhizum Pomel are used traditionally in Algeria folk medicine ... more Background: Species of Echium trygorrhizum Pomel are used traditionally in Algeria folk medicine for the treatment of Diabetes, Jaundice and Tonsillitis. To our knowledge, no previous study has been conducted on the pharmacological activities of this species. Objective: The objective of the present research was to evaluate the content of polyphenols, flavonoids and condensed tannins compounds and to assess in vitro the antioxidant activity and the inhibitory effect of the hydroalcoholic extract of this plant on α-amylase activity, an enzyme responsible for digestion of carbohydrate before the process of intestinal absorption. Methods: Polyphenols, flavonoids and condensed tannins were evaluated spectrophotometrically using Folin-Ciocalteu, the Aluminum chloride and vanillin methods, respectively. The antioxidant activity using DPPH radical scavenging, ABTS, Ferric reducing antioxidant power and β-carotene bleaching tests and the assessment of in vitro α-amylase inhibitory potential ...
Three new hydrolyzable tannins, cocciferins D(1) (1), D(2) (2), and T(1) (4), were isolated from ... more Three new hydrolyzable tannins, cocciferins D(1) (1), D(2) (2), and T(1) (4), were isolated from the leaves of Quercus coccifera. Cocciferin D(2) (2) and two additional new tannins, cocciferins D(3) (3) and T(2) (5), were also obtained from the leaves of Quercus suber. Their oligomeric structures were elucidated on the basis of spectroscopic methods and chemical evidence. Compounds 2, 3, and 5 were rare oligomers possessing glucose cores with both open-chain and pyranose forms.
The present study is designed to evaluate the antioxidant and anti-inflammatory effects of Parony... more The present study is designed to evaluate the antioxidant and anti-inflammatory effects of Paronychia argentea methanolic extract against acetic acid-induced ulcerative colitis in mice. The experimental colitis in mice was induced with 5% acetic acid (v/v, in saline) via rectal route. Animals were then divided into various treatment groups (n = 12). Four groups were treated with P. argentea methanolic extract (PAME) (50, 100, 200 and 500 mg/kg) for 5 consecutive days, and one group was treated with sulfasalazine (SASP, 500 mg/kg) as a standard drug. Disease activity index (DAI), colon weight and length, hematological parameters (C-reactive protein: CRP, erythrocyte sedimentation rate: ESR and full blood count: FBC), colonic contents of Malondialdehyde (MDA) and reduced glutathione (GSH), colonic superoxide dismutase (SOD) and catalase (CAT) activities were recorded. Results revealed that the intrarectal instillation of acetic acid caused increased DAI, colon weight, CRP, ESR and colonic MDA levels, and a decrease in the other parameters such as colon length, FBC, colonic GSH levels, SOD and CAT activities. Treatment with PAME showed significant effect in lowering DAI, colon weight, CRP, ESR and colonic MDA levels, and in enhancing the colon length, FBC, colonic GSH levels, SOD and CAT activities in a dose-dependent manner. These findings suggest that the extract obtained from P. argentea possess active substances, which exert marked protective effects in acute experimental colitis by regulating inflammatory markers and antioxidant parameters. Paronychia argentea may serve as a natural antioxidant and anti-inflammatory therapy for ulcerative colitis.
Biomolecules are largely found at different parts in divers medicinal plants. This study aims to ... more Biomolecules are largely found at different parts in divers medicinal plants. This study aims to quantify polyphenols and some biological properties of the areal part of Rubus idaeus L. plant. The shoots of Rubus idaeus L. were extracted by a methanol-water mixture. The antioxidant capacity was estimated by different in vitro assays ; linoleic acid emulsion model system, ß-carotene-linoleate model system, DPPH reduction, free radical-scavenging activity, and reducing ability assay. The highest levels of polyphenolic compounds was observed in the ethyl acetate extract (EAE). In linoleic acid test, chloroform extract (CHE) had the strongest antioxidant in comparison with butylated hydroxyl toluene (BHT) (64. 549 ± 0. 007%). In b-carotene/linoleic acid bleaching assay, the crude extract (CE) had the highest antioxidant capacity. The results showed that EAE showed the strongest antioxidant activity as measured by radicals scavenging activities and reducing power.The present work provide...
ETHNOPHARMACOLOGICAL RELEVANCE Erica arborea known as Khlenj in Algeria is a small shrub belongin... more ETHNOPHARMACOLOGICAL RELEVANCE Erica arborea known as Khlenj in Algeria is a small shrub belonging to Ericaceae family. E. arborea Aqueous extract (EAAE) is used in traditional medicine for anti-inflammatory, diuretic, antimicrobial, and antiulcer purposes. AIM OF THE STUDY To our knowledge, no data reveal the combination between in-vivo anti-inflammatory and toxicological studies of EAAE. For this purpose, the aim of this study is to evaluate the biological activity cited above and asses its safety. MATERIAL AND METHODS Anti-inflammatory activity was undergone using carrageenan-induced paw edema and croton oil-induced ear edema. The acute and sub-acute toxicity were conducted following the OECD guidelines 423 and 407 respectively. Phytochemical identification was carried out using HPLC-DAD-MS. Quantitative evaluation of polyphenols; flavonoids and antioxidant activity of EAAE were also determined. RESULTS Oral administration of EAAE (250 and 500 mg/kg) significantly (p <0.05) reduced the edema induced by carrageenan. Administration of EAAE dosed at 250 and 500 mg/kg exhibited efficacy in reducing edema induced by croton oil. The acute administration of EAAE at doses of 2000 and 5000 mg/kg did not cause any mortality or adverse effects indicating that the LD50 is above 5000 mg/kg. The prolonged administration of EAAE (500 and 1000 mg/kg) showed a significant reduction in triglycerides levels in male and female rats whereas no significant changes in other biochemical and hematological parameters were observed. Histopathological damages were recorded in both liver and kidney animal's tissues of both sexes treated with medium and maximum doses of EAAE. Phytochemical characterization of EAAE revealed a high amount of phenolic compounds, HPLC-DAD-MS analysis led to the identification of chlorogenic acid and five flavonol glycosides: myricetin pentoside, quercetin 3-O-glucoside, myricetin 3-O-rhamnoside, quercetin-3-O-pentoside, and quercetin-3-O-rhamnoside. CONCLUSION In the light of the results obtained in this study, EAAE corroborates the popular use to treat the anti-inflammatory impairments. EAAE can be considered as non-toxic in acute administration and exhibited a moderate toxicity in sub-acute administration. High phenolic content and in-vitro antioxidant activity observed indicate that EAAE may reduce oxidative stress markers in-vivo.
Centaurea species are widely used in traditional medicine to treat several illnesses, especially ... more Centaurea species are widely used in traditional medicine to treat several illnesses, especially by Mediterranean populations due to their pharmacological properties. The present study aimed to evaluate for the first time some in vivo activities of n-butanol (n-BuOH) extract of the aerial part of Centaurea tougourensis. For this approach; the antidiabetic (streptozotocin-induced diabetes), hepatoprotective (paracetamol induced hepatotoxicity), anti-inflammatory (croton oil induced ear edema assay) and antipyretic activities of this plant extract were tested. The pharmacological results suggest that C. tougourensis has a non-negligible anti-inflammatory effect on the formation of ear edema with a maximum inhibition percentage of (39.58%) for the highest tested concentration of 400 mg/kg. However, the antipyretic activity of the plant was remarkable for both tested concentrations (200 and 400 mg/kg) 5 h after treatment with a significant (P < 0.05) reduction of rectal temperature to (32.88 ± 0.23°C) and (32.36 ± 0.18°C) which correspond to a pyrexia inhibition of (78.9%) and (90.18%) respectively. C. tougourensis exhibited also a good anti-hyperglycemic effect which reached an inhibition percentage of (68.29%) at the end of the 3rd week of treatment for the tested concentration of 400 mg/kg and was considered almost similar to those of standard value (71.83%) at the same time. The n-BuOH extract C. tougourensis showed also a remarkable hepatoprotective effect which was confirmed by biochemical and histological approaches of note is that natural silymarin was also used as reference drug and showed a remarkable hepatoprotective effect. These encouraging results demonstrated once again the pharmacological potential of Centaurea species.
Background: Species of Echium trygorrhizum Pomel are used traditionally in Algeria folk medicine ... more Background: Species of Echium trygorrhizum Pomel are used traditionally in Algeria folk medicine for the treatment of Diabetes, Jaundice and Tonsillitis. To our knowledge, no previous study has been conducted on the pharmacological activities of this species. Objective: The objective of the present research was to evaluate the content of polyphenols, flavonoids and condensed tannins compounds and to assess in vitro the antioxidant activity and the inhibitory effect of the hydroalcoholic extract of this plant on α-amylase activity, an enzyme responsible for digestion of carbohydrate before the process of intestinal absorption. Methods: Polyphenols, flavonoids and condensed tannins were evaluated spectrophotometrically using Folin-Ciocalteu, the Aluminum chloride and vanillin methods, respectively. The antioxidant activity using DPPH radical scavenging, ABTS, Ferric reducing antioxidant power and β-carotene bleaching tests and the assessment of in vitro α-amylase inhibitory potential ...
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