Background Safety, tolerability and efficacy of granulocyte colony-stimulating factor (G-CSF) for... more Background Safety, tolerability and efficacy of granulocyte colony-stimulating factor (G-CSF) for mobilization of hematopoietic stem and progenitor cells (HSPCs) from healthy donors have been conclusively demonstrated. This explicitly includes, albeit for smaller cohorts and shorter observation periods, biosimilar G-CSFs. HSPC donation is non-remunerated, its sole reward being “warm glow”, hence harm to donors must be avoided with maximal certitude. To ascertain, therefore, long-term physical and mental health effects of HSPC donation, a cohort of G-CSF mobilized donors was followed longitudinally. Methods We enrolled 245 healthy volunteers in this bi-centric long-term surveillance study. 244 healthy volunteers began mobilization with twice-daily Sandoz biosimilar filgrastim and 242 underwent apheresis after G-CSF mobilization. Physical and mental health were followed up over a period of 5-years using the validated SF-12 health questionnaire. Results Baseline physical and mental hea...
2018 IEEE 27th International Symposium on Industrial Electronics (ISIE), 2018
Optimizing energy usage is becoming an economic necessity for existing buildings. Non-invasive se... more Optimizing energy usage is becoming an economic necessity for existing buildings. Non-invasive sensors and sensor networks are key technologies for efficiently achieving this goal, since it is of utmost importance that existing hydraulic systems are not changed and the engineering effort for installation remains minimal. This paper presents a data-driven approach that should allow low-cost installation of sensors at arbitrary points of the building and then retrieve the structure of the hydraulic system from the recorded sensor values. The architecture as well as first preliminary results from field test buildings are presented.
16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995, 1996
Short tandem repeat loci (STR) are polymorphic markers that can be used for human identification ... more Short tandem repeat loci (STR) are polymorphic markers that can be used for human identification in forensic or paternity casework. We have investigated the polymorphism of the STR locus D21S11 in a german population sample.
Professor Steven G. E. Marsh, Anthony Nolan Research Institute, Royal Free Hospital, Pond Street,... more Professor Steven G. E. Marsh, Anthony Nolan Research Institute, Royal Free Hospital, Pond Street, London NW3 2QG, UK.Tel: +44 20 7284 8321; Fax: +44 20 7284 8331; e-mail: steven.marsh@ucl.ac.uk; URLs: www.hla.alleles.org, www.anthonynolan.org/HIG,www.ebi.ac.uk/imgt/hladoi: 10.1111/j.1399-0039.2010.01553.x
Clinical relevance of ELISA- and single antigen bead assay (SAB)-detected pre-transplant HLA anti... more Clinical relevance of ELISA- and single antigen bead assay (SAB)-detected pre-transplant HLA antibodies (SAB-HLA-Ab) for kidney graft survival was evaluated retrospectively in 197 patients transplanted between 2002 and 2009 at the University Clinic Frankfurt. Having adjusted for re-transplantation and delayed graft function a significantly increased risk for death censored graft loss was found in patients with pre-transplant SAB-HLA-Ab (HR: 4.46; 95% Cl: 1.47 to 13.48; P=0.008). The risk for increased graft loss was also significant in patients with pre-transplant SAB-HLA-Ab but without SAB-detected donor specific Ab (SAB-DSA) (4.91; 95% CL of 1.43 - 16.991; P=0.012). ELISA was not sufficient to identify pre-transplant immunized patients with an increased risk for graft loss. In immunized patients, graft loss was predominantly present in patients who received transplants with a mismatch on the HLA-DR locus. In conclusion, even if our study is limited due to small sample size, the results show an increased risk for long-term graft loss in patients with pre-transplant SAB-HLA, even in the absence of DSA. SAB-HLA-Ab positive patients, being negative in ELISA or CDC assay, might profit from a well HLA-DR-matched graft and intensified immunosuppression. This article is protected by copyright. All rights reserved.
Abstract-As a prototype towards a single photon source we present a quantum-dot-based device, whi... more Abstract-As a prototype towards a single photon source we present a quantum-dot-based device, which is tunable and fully electrically pumped.łBoth the electrical pumping process and the manipulation of the emission wavelength of the quantum dots are independently ...
British Journal of Haematology - BRIT J HAEMATOL, 2000
The hepatitis C virus (HCV) has recently been implicated in the pathogenesis of several B-cell ly... more The hepatitis C virus (HCV) has recently been implicated in the pathogenesis of several B-cell lymphoproliferative disorders. In particular, anti-HCV antibodies and/or HCV RNA have been detected in most patients with type II mixed cryoglobulinaemia and in a large proportion of Italian patients with B-cell non-Hodgkin's lymphoma (NHL) (reviewed by Silvestri et al, 1996; Agnello, 1997; Ivanovski et al, 1998). Moreover, an asymptomatic monoclonal B-cell expansion has been demonstrated by molecular methods in < 20% of Italian patients with HCV-associated chronic liver disease (Franzin et al, 1995). The high prevalence of chronic HCV infection among patients with B-cell NHL has been observed primarily in Mediterranean countries, particularly in Italy (9±32%), where the prevalence of HCV carriers in the general population is quite high and ranges from 1% to 5 ́4%. A recent US study investigating predominantly Hispanic patients also showed a significantly higher prevalence of HCV infection in NHL patients (22%) than in age-matched controls (Zuckerman et al, 1997). However, other studies from the USA, Canada, Germany, the UK and France have not confirmed this association (reviewed by Germanidis et al, 1999). A possible explanation for this difference is the significantly lower prevalence of HCV infection in the general population of the latter countries. To test this last possibility, we investigated the prevalence of HCV infection in B-cell NHL patients from the Republic of Macedonia, which is characterized by a relatively high prevalence of HCV carriers within the general population (2 ́0%). We tested 112 consecutive patients with NHL (93 Macedonian and 19 Albanian) and a control group of 137 patients with other B-cell malignancies (38 with Hodgkin's disease, 43 with chronic lymphocytic leukaemia, nine with acute lymphoblastic leukaemia, 26 with multiple myeloma and one with WaldenstroÈm's macroglobulinaemia). The diagnosis was based on histological and immunohistochemical analysis of a lymph node and/or bone marrow biopsy according to the Revised European±American Classification (REAL) of lymphoid neoplasms. The serum samples were tested for antibodies to HCV by third-generation enzymelinked immunosorbent assay (ELISA) using commercially available kits and for HCV RNA by reverse transcriptase± polymerase chain reaction (RT±PCR) amplification of the 5 0 untranslated region of HCV. Table I summarizes the results for the different NHL subtypes and the controls. HCV infection was detected in only one patient with NHL (0 ́89%) and in one of the 137 patients with other B-cell malignancies (0 ́72%). Thus, our study demonstrates a low prevalence of HCV infection in patients with B-cell NHL from Macedonia and a lack of association between the two disorders. The comparable prevalence of HCV infection in the general population of Macedonia with that in Italy indicates that HCV infection requires additional environmental factors and/or a distinct genetic background to induce a malignant B-cell disorder. The same appears to be true for the benign HCV-associated B-cell lymphoproliferations, such as type II mixed cryoglobulinaemia, and the asymptomatic monoclonal B-cell expansions; both disorders were recently found to be significantly less prevalent in Japanese than in Italian patients with HCV-positive chronic liver disease (Pozzato et al, 1999). Therefore, it seems likely that HCV-associated lymphoproliferative disorders have a multifactorial aetiology, which includes additional, presently undetermined, genetic and environmental factors that may vary widely with geography.
HLA antigens, the major histocompatibility complex (MHC) antigens of humans, are required in disc... more HLA antigens, the major histocompatibility complex (MHC) antigens of humans, are required in discrimination by the immune system of foreign antigens from self (Unanue 1984; Bjorkman et al. 1987). The HLA class II antigens are polymorphic cell surface antigens composed of two transmembrane glycoproteins of M r 27000-29000 (/3 chain) and M r 33000-35000 (o~ chain) which associate soon after synthesis within the endoplasmic reticulum and are transported together to the surface (Kvist et al. 1982). Class II antigens are found primarily on B cells and macrophages, but are inducible in other cells by soluble factors such as interferon-3' (IFN3') and interleukin-4 (IL-4; Kaufman et al. 1984; Korman et al. 1985; Sette et al. 1987). These molecules act as restriction elements for presentation of foreign antigen to CD4-positive helper T cells and, at least in some cases, to cytotoxic T lymphocytes. In earlier studies, we showed that alternative splicing of HLA DQB1 transcripts generated distinct mRNAs which code for membrane bound and secreted DQB1 proteins (Briata et al. 1989). Furthermore, alternative splicing which deletes the transmembrane exon appeared to be associated with only a subset of DQB1 alleles. Though we have no direct evidence for their function, we suspect soluble class II molecules may contribute to the development and control of an immune response. Therefore, we set out to define more precisely allelic variation and tissue specificity in the generation of alternatively spliced DQB1 mRNAs (ATM), and to determine whether similar mRNAs for DRB transcripts could be found. For the analysis of alternative splicing, we developed an efficient and sensitive method to detect ATM and + TM mRNAs using cDNA made from total cytoplasmic RNA and amplified with specific primers using polymerase chain reaction (PCR; Mullis and Faloona 1987). HLA homozygous B lymphoblastoid cell lines
Background Safety, tolerability and efficacy of granulocyte colony-stimulating factor (G-CSF) for... more Background Safety, tolerability and efficacy of granulocyte colony-stimulating factor (G-CSF) for mobilization of hematopoietic stem and progenitor cells (HSPCs) from healthy donors have been conclusively demonstrated. This explicitly includes, albeit for smaller cohorts and shorter observation periods, biosimilar G-CSFs. HSPC donation is non-remunerated, its sole reward being “warm glow”, hence harm to donors must be avoided with maximal certitude. To ascertain, therefore, long-term physical and mental health effects of HSPC donation, a cohort of G-CSF mobilized donors was followed longitudinally. Methods We enrolled 245 healthy volunteers in this bi-centric long-term surveillance study. 244 healthy volunteers began mobilization with twice-daily Sandoz biosimilar filgrastim and 242 underwent apheresis after G-CSF mobilization. Physical and mental health were followed up over a period of 5-years using the validated SF-12 health questionnaire. Results Baseline physical and mental hea...
2018 IEEE 27th International Symposium on Industrial Electronics (ISIE), 2018
Optimizing energy usage is becoming an economic necessity for existing buildings. Non-invasive se... more Optimizing energy usage is becoming an economic necessity for existing buildings. Non-invasive sensors and sensor networks are key technologies for efficiently achieving this goal, since it is of utmost importance that existing hydraulic systems are not changed and the engineering effort for installation remains minimal. This paper presents a data-driven approach that should allow low-cost installation of sensors at arbitrary points of the building and then retrieve the structure of the hydraulic system from the recorded sensor values. The architecture as well as first preliminary results from field test buildings are presented.
16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995, 1996
Short tandem repeat loci (STR) are polymorphic markers that can be used for human identification ... more Short tandem repeat loci (STR) are polymorphic markers that can be used for human identification in forensic or paternity casework. We have investigated the polymorphism of the STR locus D21S11 in a german population sample.
Professor Steven G. E. Marsh, Anthony Nolan Research Institute, Royal Free Hospital, Pond Street,... more Professor Steven G. E. Marsh, Anthony Nolan Research Institute, Royal Free Hospital, Pond Street, London NW3 2QG, UK.Tel: +44 20 7284 8321; Fax: +44 20 7284 8331; e-mail: steven.marsh@ucl.ac.uk; URLs: www.hla.alleles.org, www.anthonynolan.org/HIG,www.ebi.ac.uk/imgt/hladoi: 10.1111/j.1399-0039.2010.01553.x
Clinical relevance of ELISA- and single antigen bead assay (SAB)-detected pre-transplant HLA anti... more Clinical relevance of ELISA- and single antigen bead assay (SAB)-detected pre-transplant HLA antibodies (SAB-HLA-Ab) for kidney graft survival was evaluated retrospectively in 197 patients transplanted between 2002 and 2009 at the University Clinic Frankfurt. Having adjusted for re-transplantation and delayed graft function a significantly increased risk for death censored graft loss was found in patients with pre-transplant SAB-HLA-Ab (HR: 4.46; 95% Cl: 1.47 to 13.48; P=0.008). The risk for increased graft loss was also significant in patients with pre-transplant SAB-HLA-Ab but without SAB-detected donor specific Ab (SAB-DSA) (4.91; 95% CL of 1.43 - 16.991; P=0.012). ELISA was not sufficient to identify pre-transplant immunized patients with an increased risk for graft loss. In immunized patients, graft loss was predominantly present in patients who received transplants with a mismatch on the HLA-DR locus. In conclusion, even if our study is limited due to small sample size, the results show an increased risk for long-term graft loss in patients with pre-transplant SAB-HLA, even in the absence of DSA. SAB-HLA-Ab positive patients, being negative in ELISA or CDC assay, might profit from a well HLA-DR-matched graft and intensified immunosuppression. This article is protected by copyright. All rights reserved.
Abstract-As a prototype towards a single photon source we present a quantum-dot-based device, whi... more Abstract-As a prototype towards a single photon source we present a quantum-dot-based device, which is tunable and fully electrically pumped.łBoth the electrical pumping process and the manipulation of the emission wavelength of the quantum dots are independently ...
British Journal of Haematology - BRIT J HAEMATOL, 2000
The hepatitis C virus (HCV) has recently been implicated in the pathogenesis of several B-cell ly... more The hepatitis C virus (HCV) has recently been implicated in the pathogenesis of several B-cell lymphoproliferative disorders. In particular, anti-HCV antibodies and/or HCV RNA have been detected in most patients with type II mixed cryoglobulinaemia and in a large proportion of Italian patients with B-cell non-Hodgkin's lymphoma (NHL) (reviewed by Silvestri et al, 1996; Agnello, 1997; Ivanovski et al, 1998). Moreover, an asymptomatic monoclonal B-cell expansion has been demonstrated by molecular methods in < 20% of Italian patients with HCV-associated chronic liver disease (Franzin et al, 1995). The high prevalence of chronic HCV infection among patients with B-cell NHL has been observed primarily in Mediterranean countries, particularly in Italy (9±32%), where the prevalence of HCV carriers in the general population is quite high and ranges from 1% to 5 ́4%. A recent US study investigating predominantly Hispanic patients also showed a significantly higher prevalence of HCV infection in NHL patients (22%) than in age-matched controls (Zuckerman et al, 1997). However, other studies from the USA, Canada, Germany, the UK and France have not confirmed this association (reviewed by Germanidis et al, 1999). A possible explanation for this difference is the significantly lower prevalence of HCV infection in the general population of the latter countries. To test this last possibility, we investigated the prevalence of HCV infection in B-cell NHL patients from the Republic of Macedonia, which is characterized by a relatively high prevalence of HCV carriers within the general population (2 ́0%). We tested 112 consecutive patients with NHL (93 Macedonian and 19 Albanian) and a control group of 137 patients with other B-cell malignancies (38 with Hodgkin's disease, 43 with chronic lymphocytic leukaemia, nine with acute lymphoblastic leukaemia, 26 with multiple myeloma and one with WaldenstroÈm's macroglobulinaemia). The diagnosis was based on histological and immunohistochemical analysis of a lymph node and/or bone marrow biopsy according to the Revised European±American Classification (REAL) of lymphoid neoplasms. The serum samples were tested for antibodies to HCV by third-generation enzymelinked immunosorbent assay (ELISA) using commercially available kits and for HCV RNA by reverse transcriptase± polymerase chain reaction (RT±PCR) amplification of the 5 0 untranslated region of HCV. Table I summarizes the results for the different NHL subtypes and the controls. HCV infection was detected in only one patient with NHL (0 ́89%) and in one of the 137 patients with other B-cell malignancies (0 ́72%). Thus, our study demonstrates a low prevalence of HCV infection in patients with B-cell NHL from Macedonia and a lack of association between the two disorders. The comparable prevalence of HCV infection in the general population of Macedonia with that in Italy indicates that HCV infection requires additional environmental factors and/or a distinct genetic background to induce a malignant B-cell disorder. The same appears to be true for the benign HCV-associated B-cell lymphoproliferations, such as type II mixed cryoglobulinaemia, and the asymptomatic monoclonal B-cell expansions; both disorders were recently found to be significantly less prevalent in Japanese than in Italian patients with HCV-positive chronic liver disease (Pozzato et al, 1999). Therefore, it seems likely that HCV-associated lymphoproliferative disorders have a multifactorial aetiology, which includes additional, presently undetermined, genetic and environmental factors that may vary widely with geography.
HLA antigens, the major histocompatibility complex (MHC) antigens of humans, are required in disc... more HLA antigens, the major histocompatibility complex (MHC) antigens of humans, are required in discrimination by the immune system of foreign antigens from self (Unanue 1984; Bjorkman et al. 1987). The HLA class II antigens are polymorphic cell surface antigens composed of two transmembrane glycoproteins of M r 27000-29000 (/3 chain) and M r 33000-35000 (o~ chain) which associate soon after synthesis within the endoplasmic reticulum and are transported together to the surface (Kvist et al. 1982). Class II antigens are found primarily on B cells and macrophages, but are inducible in other cells by soluble factors such as interferon-3' (IFN3') and interleukin-4 (IL-4; Kaufman et al. 1984; Korman et al. 1985; Sette et al. 1987). These molecules act as restriction elements for presentation of foreign antigen to CD4-positive helper T cells and, at least in some cases, to cytotoxic T lymphocytes. In earlier studies, we showed that alternative splicing of HLA DQB1 transcripts generated distinct mRNAs which code for membrane bound and secreted DQB1 proteins (Briata et al. 1989). Furthermore, alternative splicing which deletes the transmembrane exon appeared to be associated with only a subset of DQB1 alleles. Though we have no direct evidence for their function, we suspect soluble class II molecules may contribute to the development and control of an immune response. Therefore, we set out to define more precisely allelic variation and tissue specificity in the generation of alternatively spliced DQB1 mRNAs (ATM), and to determine whether similar mRNAs for DRB transcripts could be found. For the analysis of alternative splicing, we developed an efficient and sensitive method to detect ATM and + TM mRNAs using cDNA made from total cytoplasmic RNA and amplified with specific primers using polymerase chain reaction (PCR; Mullis and Faloona 1987). HLA homozygous B lymphoblastoid cell lines
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