This paper presents a modular system of wireless monitoring of physiological and ambient conditio... more This paper presents a modular system of wireless monitoring of physiological and ambient conditions based on Internet of Things (IoT). It is specially designed for elderly people and people with chronic diseases to promote their independent living. The system consists of a network of physiological and home ambient sensors developed under standard IoT protocols. These sensors are designed and manufactured at the laboratory with low-cost components. In addition, commercial sensors can be added according to the requirements of the patient. The system also provides a web based user interface for medical and family caregivers. It displays real time data and it includes an alarm system to report unsafe events via email. The caregiver can also see historical data with the possibility to compare all the parameters of a certain day and time. Furthermore, a home automation system was proposed to control home devices to improve home safety. The evaluation of the system was performed under a controlled environment at the laboratory in pilot tests before being tested in patients.
VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016, 2017
Gene Ontology is a hierarchical controlled vocabulary for protein annotation. Its synergy with au... more Gene Ontology is a hierarchical controlled vocabulary for protein annotation. Its synergy with automatic classification methods, ensemble, has been widely used for the prediction of protein functions. Current classification methods use only the relation is_a and a few little part_of to generate prediction model. In this work we formalize the GO part_of, regulates; negatively_regulates and positively_regulates relationships through predicate logic. This formalization is incorporated within an ensemble method based on graph factor called Factor Graph GO Annotation. The proposed model is validated against four model organisms for GO Biological Process prediction.
Fil: Spetale, Flavio Ezequiel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro... more Fil: Spetale, Flavio Ezequiel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Informacion y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Informacion y de Sistemas; Argentina
Many Bioinformatics tools, known as p-tools, have been developed to predict the effect of single ... more Many Bioinformatics tools, known as p-tools, have been developed to predict the effect of single nucleotide polymorphisms (SNPs) on gene functionality, in an effort to reduce the need for in-vivo assays. However, the large number of p-tools available and the heterogeneity of their output make their selection and comparison difficult. To study the consistency of predictions across p-tools, here we present two indices and test them on five p-tools whose predictions are based on different types of background information. For this test, SNPs from well-known organism Drosophila melanogaster are considered.
In this chapter, we propose a system especially created for elderly or chronically ill people tha... more In this chapter, we propose a system especially created for elderly or chronically ill people that are with special needs and poor familiarity with technology. The system combines home monitoring of physiological and emotional states through a set of wearable sensors, user-controlled (automated) home devices, and a central control for integration of the data, in order to provide a safe and friendly environment according to the limited capabilities of the users. The main objective is to create the easy, low-cost automation of a room or house to provide a friendly environment that enhances the psychological condition of immobilized users. In addition, the complete interaction of the components provides an overview of the physical and emotional state of the user, building a behavior pattern that can be supervised by the care giving staff. This approach allows the integration of physiological signals with the patient's environmental and social context to obtain a complete framework of the emotional states.
El objetivo fue evaluar algunas características morfológicas de gallinas de traspatio (n= 255) pr... more El objetivo fue evaluar algunas características morfológicas de gallinas de traspatio (n= 255) provenientes de 65 unidades rurales de producción, localizadas en 52 municipios de 18 estados de la República Mexicana. El modelo estadístico incluyó los efectos de sexo, estado y municipio anidado en estado. El efecto de sexo fue importante para todas las variables analizadas (P<0.0001), excepto para robustez (P=0.33). El efecto de estado fue importante (P<0.0001) para todas las variables analizadas, mientras que municipio solo influyó significativamente a envergadura (P<0.0001), peso corporal (P=0.0271) y solidez (P=0.0267). Los machos tuvieron mayor (P<0.0001) longitud corporal (4.85 cm), envergadura (5.66 cm), circunferencia de la pechuga (2.94 cm), longitud del tarso (1.73 cm), peso corporal (0.65 kg) y solidez (0.99 puntos porcentuales). El peso corporal aumentó 126 g (P<0.01) en machos y 61 g en hembras (P<0.01) por cada centímetro que aumentó la circunferencia de ...
The purpose of this study was to examine the genetic variability of the Mexican Simmental. Inbree... more The purpose of this study was to examine the genetic variability of the Mexican Simmental. Inbreeding was calculated by year for animals born from 1985 to 2014. Proportion of ancestors known, average equivalent complete generations, generation interval, and effective size, as well as the effective numbers of founders, ancestors, and founder genomes were calculated for animals born in six periods
The sensitivity of EPID-based transit dosimetry to detect patient variations between treatment fr... more The sensitivity of EPID-based transit dosimetry to detect patient variations between treatment fractions is examined using gamma analysis and a structural similarity (SSIM) index. EPID images were acquired for 3-dimensional conformal (3DCRT) and dynamic intensity modulated (dIMRT) radiation therapy fields in multiple fractions. Transit images were converted to doses, transit dose in the first fraction considered the reference dose. Variations in patient position or weight were then introduced in the subsequent fractions. Positional variations were examined using a lung and a head and neck phantoms. Anatomical variations were examined using a slab phantom in three scenarios, with solid water simulating tissue, medium-density fiberboard simulating fat, and Styrofoam simulating lung. The dose difference between the first and subsequent fractions was computed using various gamma criteria and the SSIM index. Using a criterion of 3%/3 mm, EPID can detect positional variations ≥ 4 mm, and tissue and fat variations ≥ 1 cm, whereas it cannot detect lung variations up to 4 cm. The sensitivity for 3DCRT is higher than for dIMRT. EPID can detect the most variations when using 3%/1 mm. With the SSIM index, EPID can detect a 2 mm positional variation and 1 cm of lung variation. The factor that optimized the sensitivity of EPID was a reduction in the distance to the agreement criteria. Our study introduces the SSIM as an alternative analysis with high sensitivity for minimal variations.
Background: In living organisms, small heat shock proteins (sHSPs) are triggered in response to s... more Background: In living organisms, small heat shock proteins (sHSPs) are triggered in response to stress situations. This family of proteins is large in plants and, in the case of tomato (Solanum lycopersicum), 33 genes have been identified, most of them related to heat stress response and to the ripening process. Transcriptomic and proteomic studies have revealed complex patterns of expression for these genes. In this work, we investigate the coregulation of these genes by performing a computational analysis of their promoter architecture to find regulatory motifs known as heat shock elements (HSEs). We leverage the presence of sHSP members that originated from tandem duplication events and analyze the promoter architecture diversity of the whole sHSP family, focusing on the identification of HSEs. Results: We performed a search for conserved genomic sequences in the promoter regions of the sHSPs of tomato, plus several other proteins (mainly HSPs) that are functionally related to heat stress situations or to ripening. Several computational analyses were performed to build multiple sequence motifs and identify transcription factor binding sites (TFBS) homologous to HSF1AE and HSF21 in Arabidopsis. We also investigated the expression and interaction of these proteins under two heat stress situations in whole tomato plants and in protoplast cells, both in the presence and in the absence of heat shock transcription factor A2 (HsfA2). The results of these analyses indicate that different sHSPs are up-regulated depending on the activation or repression of HsfA2, a key regulator of HSPs. Further, the analysis of protein-protein interaction between the sHSP protein family and other heat shock response proteins (Hsp70, Hsp90 and MBF1c) suggests that several sHSPs are mediating alternative stress response through a regulatory subnetwork that is not dependent on HsfA2. Conclusions: Overall, this study identifies two regulatory motifs (HSF1AE and HSF21) associated with the sHSP family in tomato which are considered genomic HSEs. The study also suggests that, despite the apparent redundancy of these proteins, which has been linked to gene duplication, tomato sHSPs showed different up-regulation and different interaction patterns when analyzed under different stress situations.
Background: Copy number variations are genome polymorphism that influence phenotypic variation an... more Background: Copy number variations are genome polymorphism that influence phenotypic variation and are an important source of genetic variation in populations. The aim of this study was to investigate genetic variability in the Mexican Creole chicken population using CNVs. Results: The Hidden Markov Model of the PennCNV software detected a total of 1924 CNVs in the genome of the 256 samples processed with Axiom® Genome-Wide Chicken Genotyping Array (Affymetrix). The mapped CNVs comprised 1538 gains and 386 losses, resulting at population level in 1216 CNV regions (CNVRs), of which 959 gains, 226 losses and 31 complex (i.e. containing both losses and gains). The CNVRs covered a total of 47 Mb of the whole genome sequence length, corresponding to 5.12% of the chicken galGal4 autosome assembly. Conclusions: This study allowed a deep insight into the structural variation in the genome of unselected Mexican chicken population, which up to now has not been genetically characterized. The genomic study disclosed that the population, even if presenting extreme morphological variation, cannot be organized in differentiated genetic subpopulations. Finally this study provides a chicken CNV map based on the 600 K SNP chip array jointly with a genome-wide gene copy number estimates in a native unselected for more than 500 years chicken population.
This paper presents a modular, wearable system for acquisition and wireless transmission of biolo... more This paper presents a modular, wearable system for acquisition and wireless transmission of biological signals. Configurable slaves for different signals (such as ECG, EMG, inertial sensors, and temperature) based in the ADS1294 Medical Analog Front End are connected to a Master, based in the CC3200 microcontroller, both from Texas Instruments. The slaves are configurable according to the specific application, providing versatility to the wearable system. The battery consumption is reduced, through a couple of Li-ion batteries and the circuit has also a battery charger. A custom made box was designed and fabricated in a 3D printer, preserving the requirements of low cost, low weight and safety recommendations.
The small heat shock proteins (sHSPs) have been found to play a critical role in physiological st... more The small heat shock proteins (sHSPs) have been found to play a critical role in physiological stress conditions in protecting proteins from irreversible aggregation. To characterize the gene expression profile of four sHsps with a tandem gene structure arrangement in the domesticated Solanum lycopersicum (Heinz 1706) genome and its wild close relative Solanum pimpinellifolium (LA1589), differential gene expression analysis using RNA-Seq was conducted in three ripening stages in both cultivars fruits. Gene promoter analysis was performed to explain the heterogeneous pattern of gene expression found for these tandem duplicated sHsps. In silico analysis results contribute to refocus wet experiment analysis in tomato sHsp family proteins.
Advances in medicine have led to a significant increase in human life expectancy and, therefore, ... more Advances in medicine have led to a significant increase in human life expectancy and, therefore, to a growing number of disabled elderly people who need chronic care and assistance [1]. The World Health Organization reports that the world's population over 60 years old will double between 2000 and 2050 and quadruple for seniors older than 80 years, reaching 400 million [2]. In addition, strokes, traffic-related and other accidents, and seemingly endless wars and acts of terrorism contribute to an increasing number of disabled younger people.
Gene Ontology (GO) is a hierarchical vocabulary for gene product annotation. Its synergy with mac... more Gene Ontology (GO) is a hierarchical vocabulary for gene product annotation. Its synergy with machine learning classification methods has been widely used for the prediction of protein functions. Current classification methods rely on heuristic solutions to check the consistency with some aspects of the underlying GO structure. In this work we formalize the GO is-a relationship through predicate logic. Moreover, an ontology model based on Forney Factor Graph (FFG) is shown on a general fragment of Cellular Component GO.
Mind and consciousness are like a gradual sunrise that takes time to come up. In the meantime, we... more Mind and consciousness are like a gradual sunrise that takes time to come up. In the meantime, we are not stagnant, waiting for life to happen. The music of life, felt with our bodies, long before we identify mind with self...some of us stay here longer than others. Music in its broader sense is everywhere, and it is our first language...it has the most important of all places in the lives of those who find the realm of the mind a place of rusty cogs and heavy effort.
Abstract Defined as the introduction of an object into the digestive tract. Approximately 55% of ... more Abstract Defined as the introduction of an object into the digestive tract. Approximately 55% of the objects that children put into their mouth and swallow will be excreted. Ingestion is a frequent accident, and the nature of the object, its size and the time it has remained in the child’s system shall determine the treatment to be followed. The majority of ingested objects are inorganic (coins, toy parts, button batteries), and some are organic (food remnants). The body has 4 natural strictures, the most significant occurring at the cricopharyngeal segment. If the object is able to pass this point, it normally is excreted, unless there is another unknown pathologic stricture (e.g. stenosis due to reflux). Extraction is undertaken by means of direct laryngoscopy and fine-point forceps, may require rigid or flexible endoscopy and a small percentage of patients are kept in the hospital for observation. In the case of button batteries, immediate extraction or surgery is warranted if th...
Abstract Foreign body aspiration into the airways is defined as the accidental introduction to th... more Abstract Foreign body aspiration into the airways is defined as the accidental introduction to the larynx, trachea and bronchia of an organic or inorganic object that affects the child’s respiration. This is a potentially fatal event. It is a common accident in children under 2 years of age, and it ranks fourth among the causes of mortality by asphyxia in children. The use of bronchoscopic techniques in children has significantly reduced mortality rates.The inhaled foreign bodies can be classified as organic or inorganic, with the former being the most common Infants are at risk due to their exploring instinct, mouthing or the habit of putting objects into their mouths, inadequate or inapt swallowing, and parents’ negligence Clinically, foreign body aspiration is suspected upon the sudden onset of choking symptoms, followed by stridor, difficult breathing and fever, and may be confused with respiratory infections or asthma attacks. Some objects may remain lodged in the airways even ...
VI Latin American Congress on Biomedical Engineering CLAIB 2014, Paraná, Argentina 29, 30 & 31 October 2014, 2015
Small Heat Shock Proteins (sHSPs) are low molecular weight chaperones that play an important role... more Small Heat Shock Proteins (sHSPs) are low molecular weight chaperones that play an important role during stress response and development in all living organisms. Fruit maturation and oxidative stress can induce sHSP synthesis both in Arabidopsis and tomato plants. RNA-Seq technology is becoming widely used in various transcriptomics studies; however, analyzing and interpreting the RNA-Seq data face serious challenges. In the present work, we de novo assembled the Solanum lycopersicum transcriptome for three different maturation stages (mature green, breaker and red ripe). Differential gene expression analysis was carried out during tomato fruit development. We identified sHSPs differentially expressed that might be involved in breaker and red ripe fruit maturation. Interestingly, these sHSPs have different subcellular localization and suggest a complex regulation of the fruit maturation network process.
This paper presents a modular system of wireless monitoring of physiological and ambient conditio... more This paper presents a modular system of wireless monitoring of physiological and ambient conditions based on Internet of Things (IoT). It is specially designed for elderly people and people with chronic diseases to promote their independent living. The system consists of a network of physiological and home ambient sensors developed under standard IoT protocols. These sensors are designed and manufactured at the laboratory with low-cost components. In addition, commercial sensors can be added according to the requirements of the patient. The system also provides a web based user interface for medical and family caregivers. It displays real time data and it includes an alarm system to report unsafe events via email. The caregiver can also see historical data with the possibility to compare all the parameters of a certain day and time. Furthermore, a home automation system was proposed to control home devices to improve home safety. The evaluation of the system was performed under a controlled environment at the laboratory in pilot tests before being tested in patients.
VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016, 2017
Gene Ontology is a hierarchical controlled vocabulary for protein annotation. Its synergy with au... more Gene Ontology is a hierarchical controlled vocabulary for protein annotation. Its synergy with automatic classification methods, ensemble, has been widely used for the prediction of protein functions. Current classification methods use only the relation is_a and a few little part_of to generate prediction model. In this work we formalize the GO part_of, regulates; negatively_regulates and positively_regulates relationships through predicate logic. This formalization is incorporated within an ensemble method based on graph factor called Factor Graph GO Annotation. The proposed model is validated against four model organisms for GO Biological Process prediction.
Fil: Spetale, Flavio Ezequiel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro... more Fil: Spetale, Flavio Ezequiel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Informacion y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Informacion y de Sistemas; Argentina
Many Bioinformatics tools, known as p-tools, have been developed to predict the effect of single ... more Many Bioinformatics tools, known as p-tools, have been developed to predict the effect of single nucleotide polymorphisms (SNPs) on gene functionality, in an effort to reduce the need for in-vivo assays. However, the large number of p-tools available and the heterogeneity of their output make their selection and comparison difficult. To study the consistency of predictions across p-tools, here we present two indices and test them on five p-tools whose predictions are based on different types of background information. For this test, SNPs from well-known organism Drosophila melanogaster are considered.
In this chapter, we propose a system especially created for elderly or chronically ill people tha... more In this chapter, we propose a system especially created for elderly or chronically ill people that are with special needs and poor familiarity with technology. The system combines home monitoring of physiological and emotional states through a set of wearable sensors, user-controlled (automated) home devices, and a central control for integration of the data, in order to provide a safe and friendly environment according to the limited capabilities of the users. The main objective is to create the easy, low-cost automation of a room or house to provide a friendly environment that enhances the psychological condition of immobilized users. In addition, the complete interaction of the components provides an overview of the physical and emotional state of the user, building a behavior pattern that can be supervised by the care giving staff. This approach allows the integration of physiological signals with the patient's environmental and social context to obtain a complete framework of the emotional states.
El objetivo fue evaluar algunas características morfológicas de gallinas de traspatio (n= 255) pr... more El objetivo fue evaluar algunas características morfológicas de gallinas de traspatio (n= 255) provenientes de 65 unidades rurales de producción, localizadas en 52 municipios de 18 estados de la República Mexicana. El modelo estadístico incluyó los efectos de sexo, estado y municipio anidado en estado. El efecto de sexo fue importante para todas las variables analizadas (P<0.0001), excepto para robustez (P=0.33). El efecto de estado fue importante (P<0.0001) para todas las variables analizadas, mientras que municipio solo influyó significativamente a envergadura (P<0.0001), peso corporal (P=0.0271) y solidez (P=0.0267). Los machos tuvieron mayor (P<0.0001) longitud corporal (4.85 cm), envergadura (5.66 cm), circunferencia de la pechuga (2.94 cm), longitud del tarso (1.73 cm), peso corporal (0.65 kg) y solidez (0.99 puntos porcentuales). El peso corporal aumentó 126 g (P<0.01) en machos y 61 g en hembras (P<0.01) por cada centímetro que aumentó la circunferencia de ...
The purpose of this study was to examine the genetic variability of the Mexican Simmental. Inbree... more The purpose of this study was to examine the genetic variability of the Mexican Simmental. Inbreeding was calculated by year for animals born from 1985 to 2014. Proportion of ancestors known, average equivalent complete generations, generation interval, and effective size, as well as the effective numbers of founders, ancestors, and founder genomes were calculated for animals born in six periods
The sensitivity of EPID-based transit dosimetry to detect patient variations between treatment fr... more The sensitivity of EPID-based transit dosimetry to detect patient variations between treatment fractions is examined using gamma analysis and a structural similarity (SSIM) index. EPID images were acquired for 3-dimensional conformal (3DCRT) and dynamic intensity modulated (dIMRT) radiation therapy fields in multiple fractions. Transit images were converted to doses, transit dose in the first fraction considered the reference dose. Variations in patient position or weight were then introduced in the subsequent fractions. Positional variations were examined using a lung and a head and neck phantoms. Anatomical variations were examined using a slab phantom in three scenarios, with solid water simulating tissue, medium-density fiberboard simulating fat, and Styrofoam simulating lung. The dose difference between the first and subsequent fractions was computed using various gamma criteria and the SSIM index. Using a criterion of 3%/3 mm, EPID can detect positional variations ≥ 4 mm, and tissue and fat variations ≥ 1 cm, whereas it cannot detect lung variations up to 4 cm. The sensitivity for 3DCRT is higher than for dIMRT. EPID can detect the most variations when using 3%/1 mm. With the SSIM index, EPID can detect a 2 mm positional variation and 1 cm of lung variation. The factor that optimized the sensitivity of EPID was a reduction in the distance to the agreement criteria. Our study introduces the SSIM as an alternative analysis with high sensitivity for minimal variations.
Background: In living organisms, small heat shock proteins (sHSPs) are triggered in response to s... more Background: In living organisms, small heat shock proteins (sHSPs) are triggered in response to stress situations. This family of proteins is large in plants and, in the case of tomato (Solanum lycopersicum), 33 genes have been identified, most of them related to heat stress response and to the ripening process. Transcriptomic and proteomic studies have revealed complex patterns of expression for these genes. In this work, we investigate the coregulation of these genes by performing a computational analysis of their promoter architecture to find regulatory motifs known as heat shock elements (HSEs). We leverage the presence of sHSP members that originated from tandem duplication events and analyze the promoter architecture diversity of the whole sHSP family, focusing on the identification of HSEs. Results: We performed a search for conserved genomic sequences in the promoter regions of the sHSPs of tomato, plus several other proteins (mainly HSPs) that are functionally related to heat stress situations or to ripening. Several computational analyses were performed to build multiple sequence motifs and identify transcription factor binding sites (TFBS) homologous to HSF1AE and HSF21 in Arabidopsis. We also investigated the expression and interaction of these proteins under two heat stress situations in whole tomato plants and in protoplast cells, both in the presence and in the absence of heat shock transcription factor A2 (HsfA2). The results of these analyses indicate that different sHSPs are up-regulated depending on the activation or repression of HsfA2, a key regulator of HSPs. Further, the analysis of protein-protein interaction between the sHSP protein family and other heat shock response proteins (Hsp70, Hsp90 and MBF1c) suggests that several sHSPs are mediating alternative stress response through a regulatory subnetwork that is not dependent on HsfA2. Conclusions: Overall, this study identifies two regulatory motifs (HSF1AE and HSF21) associated with the sHSP family in tomato which are considered genomic HSEs. The study also suggests that, despite the apparent redundancy of these proteins, which has been linked to gene duplication, tomato sHSPs showed different up-regulation and different interaction patterns when analyzed under different stress situations.
Background: Copy number variations are genome polymorphism that influence phenotypic variation an... more Background: Copy number variations are genome polymorphism that influence phenotypic variation and are an important source of genetic variation in populations. The aim of this study was to investigate genetic variability in the Mexican Creole chicken population using CNVs. Results: The Hidden Markov Model of the PennCNV software detected a total of 1924 CNVs in the genome of the 256 samples processed with Axiom® Genome-Wide Chicken Genotyping Array (Affymetrix). The mapped CNVs comprised 1538 gains and 386 losses, resulting at population level in 1216 CNV regions (CNVRs), of which 959 gains, 226 losses and 31 complex (i.e. containing both losses and gains). The CNVRs covered a total of 47 Mb of the whole genome sequence length, corresponding to 5.12% of the chicken galGal4 autosome assembly. Conclusions: This study allowed a deep insight into the structural variation in the genome of unselected Mexican chicken population, which up to now has not been genetically characterized. The genomic study disclosed that the population, even if presenting extreme morphological variation, cannot be organized in differentiated genetic subpopulations. Finally this study provides a chicken CNV map based on the 600 K SNP chip array jointly with a genome-wide gene copy number estimates in a native unselected for more than 500 years chicken population.
This paper presents a modular, wearable system for acquisition and wireless transmission of biolo... more This paper presents a modular, wearable system for acquisition and wireless transmission of biological signals. Configurable slaves for different signals (such as ECG, EMG, inertial sensors, and temperature) based in the ADS1294 Medical Analog Front End are connected to a Master, based in the CC3200 microcontroller, both from Texas Instruments. The slaves are configurable according to the specific application, providing versatility to the wearable system. The battery consumption is reduced, through a couple of Li-ion batteries and the circuit has also a battery charger. A custom made box was designed and fabricated in a 3D printer, preserving the requirements of low cost, low weight and safety recommendations.
The small heat shock proteins (sHSPs) have been found to play a critical role in physiological st... more The small heat shock proteins (sHSPs) have been found to play a critical role in physiological stress conditions in protecting proteins from irreversible aggregation. To characterize the gene expression profile of four sHsps with a tandem gene structure arrangement in the domesticated Solanum lycopersicum (Heinz 1706) genome and its wild close relative Solanum pimpinellifolium (LA1589), differential gene expression analysis using RNA-Seq was conducted in three ripening stages in both cultivars fruits. Gene promoter analysis was performed to explain the heterogeneous pattern of gene expression found for these tandem duplicated sHsps. In silico analysis results contribute to refocus wet experiment analysis in tomato sHsp family proteins.
Advances in medicine have led to a significant increase in human life expectancy and, therefore, ... more Advances in medicine have led to a significant increase in human life expectancy and, therefore, to a growing number of disabled elderly people who need chronic care and assistance [1]. The World Health Organization reports that the world's population over 60 years old will double between 2000 and 2050 and quadruple for seniors older than 80 years, reaching 400 million [2]. In addition, strokes, traffic-related and other accidents, and seemingly endless wars and acts of terrorism contribute to an increasing number of disabled younger people.
Gene Ontology (GO) is a hierarchical vocabulary for gene product annotation. Its synergy with mac... more Gene Ontology (GO) is a hierarchical vocabulary for gene product annotation. Its synergy with machine learning classification methods has been widely used for the prediction of protein functions. Current classification methods rely on heuristic solutions to check the consistency with some aspects of the underlying GO structure. In this work we formalize the GO is-a relationship through predicate logic. Moreover, an ontology model based on Forney Factor Graph (FFG) is shown on a general fragment of Cellular Component GO.
Mind and consciousness are like a gradual sunrise that takes time to come up. In the meantime, we... more Mind and consciousness are like a gradual sunrise that takes time to come up. In the meantime, we are not stagnant, waiting for life to happen. The music of life, felt with our bodies, long before we identify mind with self...some of us stay here longer than others. Music in its broader sense is everywhere, and it is our first language...it has the most important of all places in the lives of those who find the realm of the mind a place of rusty cogs and heavy effort.
Abstract Defined as the introduction of an object into the digestive tract. Approximately 55% of ... more Abstract Defined as the introduction of an object into the digestive tract. Approximately 55% of the objects that children put into their mouth and swallow will be excreted. Ingestion is a frequent accident, and the nature of the object, its size and the time it has remained in the child’s system shall determine the treatment to be followed. The majority of ingested objects are inorganic (coins, toy parts, button batteries), and some are organic (food remnants). The body has 4 natural strictures, the most significant occurring at the cricopharyngeal segment. If the object is able to pass this point, it normally is excreted, unless there is another unknown pathologic stricture (e.g. stenosis due to reflux). Extraction is undertaken by means of direct laryngoscopy and fine-point forceps, may require rigid or flexible endoscopy and a small percentage of patients are kept in the hospital for observation. In the case of button batteries, immediate extraction or surgery is warranted if th...
Abstract Foreign body aspiration into the airways is defined as the accidental introduction to th... more Abstract Foreign body aspiration into the airways is defined as the accidental introduction to the larynx, trachea and bronchia of an organic or inorganic object that affects the child’s respiration. This is a potentially fatal event. It is a common accident in children under 2 years of age, and it ranks fourth among the causes of mortality by asphyxia in children. The use of bronchoscopic techniques in children has significantly reduced mortality rates.The inhaled foreign bodies can be classified as organic or inorganic, with the former being the most common Infants are at risk due to their exploring instinct, mouthing or the habit of putting objects into their mouths, inadequate or inapt swallowing, and parents’ negligence Clinically, foreign body aspiration is suspected upon the sudden onset of choking symptoms, followed by stridor, difficult breathing and fever, and may be confused with respiratory infections or asthma attacks. Some objects may remain lodged in the airways even ...
VI Latin American Congress on Biomedical Engineering CLAIB 2014, Paraná, Argentina 29, 30 & 31 October 2014, 2015
Small Heat Shock Proteins (sHSPs) are low molecular weight chaperones that play an important role... more Small Heat Shock Proteins (sHSPs) are low molecular weight chaperones that play an important role during stress response and development in all living organisms. Fruit maturation and oxidative stress can induce sHSP synthesis both in Arabidopsis and tomato plants. RNA-Seq technology is becoming widely used in various transcriptomics studies; however, analyzing and interpreting the RNA-Seq data face serious challenges. In the present work, we de novo assembled the Solanum lycopersicum transcriptome for three different maturation stages (mature green, breaker and red ripe). Differential gene expression analysis was carried out during tomato fruit development. We identified sHSPs differentially expressed that might be involved in breaker and red ripe fruit maturation. Interestingly, these sHSPs have different subcellular localization and suggest a complex regulation of the fruit maturation network process.
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Papers by Sergio Torres Ponce