International Journal of Current Science Research and Review
In the present study biodegradation of Polycyclic Aromatic Hydrocarbon (PAH) compound Naphthalene... more In the present study biodegradation of Polycyclic Aromatic Hydrocarbon (PAH) compound Naphthalene by four bacterial cultures Bacillus subtilis PD6, Bacillussp. PD9, Enterobactersp. PD11 and Bacillussp. PD14 has been targeted. Biodegradation of Naphthalene by these four selected bacterial cultures was analysed by HPLC (High Performance Liquid Chromatography) technique. HPLC analysis revealed biodegradation of naphthalene by all the four bacterial cultures within a span of six days. Highest biodegradation 78.1% has been shown by Bacillus subtilis PD6 while other bacterial cultures Bacillus sp. PD9 has shown 77.90%, Enterobacter sp. PD11 showed 74.4% and Bacillus sp. PD14 exhibited 73.5% biodegradation of naphthalene.
International Journal of Advanced Research in Science, Communication and Technology
In the present study biodegradation of used engine oil by four bacterial cultures was targeted. V... more In the present study biodegradation of used engine oil by four bacterial cultures was targeted. Versatile catabolic capability of four bacterial cultures Bacillus subtilis PD6, Bacillus sp. PD9, Enterobacter sp. PD11 and Bacillus sp. PD14 was assessed by subjecting them for the utilization of used engine oil in 250ml 0.1XM9 media containing flasks. Biodegradation of used engine oil was analysed by COD analysis and observation were statistically processed by ANOVA. Out of four bacterial cultures, Bacillus sp. PD9 and Bacillus sp. PD14 were found to be efficient cultures which were able to degrade 67.8% and 65.5% used engine oil within six days. Other two cultures Bacillus subtilis PD6 and Enterobacter sp. PD11 also exhibited successful biodegradation of used engine oil but less efficiently compared to Bacillus sp. PD9 and Bacillus sp. PD14. This study accentuate the role of isolated four bacterial cultures in removal of used engine oil and its possible application in bioremediation s...
International Journal of Current Science Research and Review
In the present study biodegradation of alphatic hycarbon compounds by bacterial cultures has been... more In the present study biodegradation of alphatic hycarbon compounds by bacterial cultures has been targeted. Growth curves analysis of Bacillus subtilis PD6, Bacillus sp. PD9, Enterobacter sp. PD11 and Bacillus sp. PD14 during utilization of dodecane, hexadecane, octadecane, eicosane and tetracosane as a sole source of carbon for growth and energy was performed. During this study, the degradation of different aliphatic hydrocarbons was studied as a function of bacterial growth. The hydrocarbon degradation efficacy of different bacterial species (selected on the basis of preliminary screening studies) was determined by indirect method wherein, the change in COD (Chemical Oxygen Demand) was determined after a specific time interval (0 day to 6th day). Presence of aliphatic hydrocarbon degradation capability in the selected four bacterial cultures was substantiated by the PCR amplification of alkB genetic loci in three out of four cultures. Successful amplification of alkB gene loci in ...
Hepatoprotective activity of Tridax procumbens L. a medicinal herb commonly used in folklore syst... more Hepatoprotective activity of Tridax procumbens L. a medicinal herb commonly used in folklore system for wound healing and also against jaundice, was evaluated against paracetamol (acetaminophen) induced hepatic damage in male albino rats. Paracetamol (2gm/kg body weight) induced hepatic damage was well manifested by significant increase in the activities of Alanine aminotransferase, Aspartate aminotransferase, Alkaline phosphatase in serum and enhanced lipid peroxidation. On the other hand, the activities of Superoxide dismutase and Catalase in liver tissue were lowered. Consequent to paracetamol induced hepatic injury, the Serum Bilirubin level was increased. Paracetamol toxicity, also resulted in, significant reduction in total serum protein and the hepatic glutathione and glycogen contents. The oral administration of varying doses of ethanolic extract of Tridax procumbens L. (100, 200, 300 and 400mg/kg body weight) for the period of 7 days reversed these altered parameters to nor...
International Journal of Current Science Research and Review
In the present study biodegradation of Polycyclic Aromatic Hydrocarbon (PAH) compound Naphthalene... more In the present study biodegradation of Polycyclic Aromatic Hydrocarbon (PAH) compound Naphthalene by four bacterial cultures Bacillus subtilis PD6, Bacillussp. PD9, Enterobactersp. PD11 and Bacillussp. PD14 has been targeted. Biodegradation of Naphthalene by these four selected bacterial cultures was analysed by HPLC (High Performance Liquid Chromatography) technique. HPLC analysis revealed biodegradation of naphthalene by all the four bacterial cultures within a span of six days. Highest biodegradation 78.1% has been shown by Bacillus subtilis PD6 while other bacterial cultures Bacillus sp. PD9 has shown 77.90%, Enterobacter sp. PD11 showed 74.4% and Bacillus sp. PD14 exhibited 73.5% biodegradation of naphthalene.
International Journal of Advanced Research in Science, Communication and Technology
In the present study biodegradation of used engine oil by four bacterial cultures was targeted. V... more In the present study biodegradation of used engine oil by four bacterial cultures was targeted. Versatile catabolic capability of four bacterial cultures Bacillus subtilis PD6, Bacillus sp. PD9, Enterobacter sp. PD11 and Bacillus sp. PD14 was assessed by subjecting them for the utilization of used engine oil in 250ml 0.1XM9 media containing flasks. Biodegradation of used engine oil was analysed by COD analysis and observation were statistically processed by ANOVA. Out of four bacterial cultures, Bacillus sp. PD9 and Bacillus sp. PD14 were found to be efficient cultures which were able to degrade 67.8% and 65.5% used engine oil within six days. Other two cultures Bacillus subtilis PD6 and Enterobacter sp. PD11 also exhibited successful biodegradation of used engine oil but less efficiently compared to Bacillus sp. PD9 and Bacillus sp. PD14. This study accentuate the role of isolated four bacterial cultures in removal of used engine oil and its possible application in bioremediation s...
International Journal of Current Science Research and Review
In the present study biodegradation of alphatic hycarbon compounds by bacterial cultures has been... more In the present study biodegradation of alphatic hycarbon compounds by bacterial cultures has been targeted. Growth curves analysis of Bacillus subtilis PD6, Bacillus sp. PD9, Enterobacter sp. PD11 and Bacillus sp. PD14 during utilization of dodecane, hexadecane, octadecane, eicosane and tetracosane as a sole source of carbon for growth and energy was performed. During this study, the degradation of different aliphatic hydrocarbons was studied as a function of bacterial growth. The hydrocarbon degradation efficacy of different bacterial species (selected on the basis of preliminary screening studies) was determined by indirect method wherein, the change in COD (Chemical Oxygen Demand) was determined after a specific time interval (0 day to 6th day). Presence of aliphatic hydrocarbon degradation capability in the selected four bacterial cultures was substantiated by the PCR amplification of alkB genetic loci in three out of four cultures. Successful amplification of alkB gene loci in ...
Hepatoprotective activity of Tridax procumbens L. a medicinal herb commonly used in folklore syst... more Hepatoprotective activity of Tridax procumbens L. a medicinal herb commonly used in folklore system for wound healing and also against jaundice, was evaluated against paracetamol (acetaminophen) induced hepatic damage in male albino rats. Paracetamol (2gm/kg body weight) induced hepatic damage was well manifested by significant increase in the activities of Alanine aminotransferase, Aspartate aminotransferase, Alkaline phosphatase in serum and enhanced lipid peroxidation. On the other hand, the activities of Superoxide dismutase and Catalase in liver tissue were lowered. Consequent to paracetamol induced hepatic injury, the Serum Bilirubin level was increased. Paracetamol toxicity, also resulted in, significant reduction in total serum protein and the hepatic glutathione and glycogen contents. The oral administration of varying doses of ethanolic extract of Tridax procumbens L. (100, 200, 300 and 400mg/kg body weight) for the period of 7 days reversed these altered parameters to nor...
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