The purpose of preserving and maintaining bacterial cultures in a university setting is to ensure... more The purpose of preserving and maintaining bacterial cultures in a university setting is to ensure they remain viable for laboratory classes and research, and generate consistent laboratory test results over time. Pseudomonas aeruginosa can be preserved by refrigeration at 4°C, but this is only suitable for short-term storage (2-3 weeks). Longer-term storage can be achieved if the bacteria being stored at 4°C are periodically sub-cultured. This is known as the 'periodic transfer method', but it has the disadvantage of failing to prevent changes in the characteristics of the strain. Changes can arise due to genetic drift or contamination. In the present study, we investigated three categories of method for preserving P. aeruginosa: (a) periodic transfer to fresh media and refrigeration and (b) storage in mineral oil or liquid paraffin and refrigeration and (c) freezing with 15% (v/v) glycerol, followed by retrieval of bacterial culture from frozen stock. Biochemical tests were...
Intestinal parasitic infections remain a serious public-health problem in Thailand. Most infectio... more Intestinal parasitic infections remain a serious public-health problem in Thailand. Most infectionsresult from fecal-oral transmission by ingestion of contaminated water and food, the major vehiclesfor transmitting infection. This study examined the prevalence of intestinal parasitic infections in435 stool samples (villagers living in Ban Nong Um = 177, villagers living in Ban Khawao Yai = 98, andteachers living in Amphoe Mueang = 160) in Mahasarakham Province, by formalin-ether concentrationtechnique. The overall prevalence of intestinal parasitic infections in the 435 stool samples was14.71% (64/435). The highest prevalence was found among villagers living in Ban Khawao Yaiwith an infection rate of 30.60%, compared with 12.99% and 6.88% among villagers living in BanNong Um and teachers in Amphoe Mueang, respectively. Strongyloides stercoralis infection (6.44%) wasthe most frequent pathogenic intestinal parasitic infection, followed by Opisthorchis viverrini (5.74%)and Taenia sp (1...
Intestinal parasitic infections continue to represent a serious public health problem in Thailand... more Intestinal parasitic infections continue to represent a serious public health problem in Thailand.Most infections result from fecal-oral transmission, with the ingestion of contaminated water orfood being the major vehicles of infection. We studied the prevalence of intestinal parasitic infectionsin 331 stool samples (Ban Wang Bua, Tambon Kham Thao Phatthana, Amphoe Kantharawichai, MahaSarakham Province = 227; Tambon Nong Bua, Amphoe Nong Kung Si, Kalasin Province = 104) byformalin-ether concentration technique. The overall prevalence of intestinal parasitic infection was29.30% (97/331). The prevalence of intestinal parasitic infection in Tambon Nong Bua in KalasinProvince was higher than Ban Wang Bua, Tambon Kham Thao Phattana, in Maha Sarakham Province(33.65% vs 27.31%). Opisthorchis viverrini infection (13.90%) was the most common pathogenicintestinal parasitic infection, followed by Strongyloides stercoralis (11.47%) and Taenia sp (1.81%).In conclusion, instruction in personnel ...
The purpose of preserving and maintaining bacterial cultures in a university setting is to ensure... more The purpose of preserving and maintaining bacterial cultures in a university setting is to ensure they remain viable for laboratory classes and research, and generate consistent laboratory test results over time. Pseudomonas aeruginosa can be preserved by refrigeration at 4°C, but this is only suitable for short-term storage (2-3 weeks). Longer-term storage can be achieved if the bacteria being stored at 4°C are periodically sub-cultured. This is known as the 'periodic transfer method', but it has the disadvantage of failing to prevent changes in the characteristics of the strain. Changes can arise due to genetic drift or contamination. In the present study, we investigated three categories of method for preserving P. aeruginosa: (a) periodic transfer to fresh media and refrigeration and (b) storage in mineral oil or liquid paraffin and refrigeration and (c) freezing with 15% (v/v) glycerol, followed by retrieval of bacterial culture from frozen stock. Biochemical tests were...
Intestinal parasitic infections remain a serious public-health problem in Thailand. Most infectio... more Intestinal parasitic infections remain a serious public-health problem in Thailand. Most infectionsresult from fecal-oral transmission by ingestion of contaminated water and food, the major vehiclesfor transmitting infection. This study examined the prevalence of intestinal parasitic infections in435 stool samples (villagers living in Ban Nong Um = 177, villagers living in Ban Khawao Yai = 98, andteachers living in Amphoe Mueang = 160) in Mahasarakham Province, by formalin-ether concentrationtechnique. The overall prevalence of intestinal parasitic infections in the 435 stool samples was14.71% (64/435). The highest prevalence was found among villagers living in Ban Khawao Yaiwith an infection rate of 30.60%, compared with 12.99% and 6.88% among villagers living in BanNong Um and teachers in Amphoe Mueang, respectively. Strongyloides stercoralis infection (6.44%) wasthe most frequent pathogenic intestinal parasitic infection, followed by Opisthorchis viverrini (5.74%)and Taenia sp (1...
Intestinal parasitic infections continue to represent a serious public health problem in Thailand... more Intestinal parasitic infections continue to represent a serious public health problem in Thailand.Most infections result from fecal-oral transmission, with the ingestion of contaminated water orfood being the major vehicles of infection. We studied the prevalence of intestinal parasitic infectionsin 331 stool samples (Ban Wang Bua, Tambon Kham Thao Phatthana, Amphoe Kantharawichai, MahaSarakham Province = 227; Tambon Nong Bua, Amphoe Nong Kung Si, Kalasin Province = 104) byformalin-ether concentration technique. The overall prevalence of intestinal parasitic infection was29.30% (97/331). The prevalence of intestinal parasitic infection in Tambon Nong Bua in KalasinProvince was higher than Ban Wang Bua, Tambon Kham Thao Phattana, in Maha Sarakham Province(33.65% vs 27.31%). Opisthorchis viverrini infection (13.90%) was the most common pathogenicintestinal parasitic infection, followed by Strongyloides stercoralis (11.47%) and Taenia sp (1.81%).In conclusion, instruction in personnel ...
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