Background: Despite the best efforts for improving the greengram varieties, the yield potential o... more Background: Despite the best efforts for improving the greengram varieties, the yield potential of this crop remains low owing to both biotic and abiotic factors. Yield losses caused due to various diseases were estimated to be 24-67 per cent in anthracnose, 80-100 per cent in mungbean yellow mosaic virus and 40 per cent due to powdery mildew So, the use of resistant cultivars is the most effective and eco-friendly method of managing the disease. Methods: A series of field experiments spanned over seven years (2014-2021) were conducted at Main Agricultural Research Station, University of Agricultural Sciences, Dharwad, Karnataka. In the first set of experiments, the material comprised of eight varieties of greengram which were treated with 20kR gamma radiation. M1was planted in Kharif 2014 and each variety was bulk harvested. M2 was planted in summer 2014 and individual plant selection was carried out. Mutant families were advanced to the further generation in Kharif 2015 and Rabi 2...
The present investigation was carried out to understand the role of different genotypes with its ... more The present investigation was carried out to understand the role of different genotypes with its surrounding environments interaction in the expression of various characters and stability over successive seasons. The phenotypic stability of genotypes were estimated by mean performance over seasons, the regression coefficient (bi) and deviation from regression. Based on stability parameters the genotypes DGGV-25 and DGGV-40 exhibited moderate mean performance for seeds yield per plant with regression value closer to unity and non-significant deviation from regression, indicating high stability and wider adaptability across three environments. These genotypes also confer Moderate Resistance to MYMV and their performance could be checked under Multi location trials, which may also serve as better source for MYMV resistance..
Background: Mungbean yellow mosaic virus disease is the most devastating disease on Mungbean prod... more Background: Mungbean yellow mosaic virus disease is the most devastating disease on Mungbean production. The virus is transmitted by whitefly and can cause yield losses from 75 to 100 per cent. The development of mungbean cultivars resistant to both virus and its vector is considered as one of the most desirable means of managing the disease as it is environmentally safe and highly efficient. The selection of resistant genotypes in conventional methods is complex and time consuming. Hence, the use of molecular markers linked with resistance genes is powerful as it hastens the breeding programmes. The current study was aimed to develop mapping population and to validate molecular markers associated with Mungbean yellow mosaic virus (MYMV). Methods: The present investigation was carried out with 260 F2 individuals that were derived from crossing DGGV-2 and IPM 2-14 during Kharif-2017 at Main Agril Research Station, UAS, Dharwad. Hybrid seeds of this cross were harvested individually a...
Genetically engineered resistance has been actively investigated in recent years as an alternativ... more Genetically engineered resistance has been actively investigated in recent years as an alternative for genes from wild relatives. The development of a method to obtain transformants, which is independent of problems inherent to tissue culture procedures, has been the ultimate goal of plant breeders. The alternative method, which involves Agrobacterium, but does not require tissue culture steps, is in planta tansformation. Although use of pollen tube pathway to deliver foreign DNA in to embryo sacs first reported in cotton was encouraging the overall quality of the data was very weak. Therefore, our attempts to modify this method yielded fruitful results. Out of 5 619 flowers, which were treated with Agrobacterium, where in the stigmatic surface was treated with 5% sucrose along with 20 ppm boric acid prior to application of Agrobacterium resulted in a boll set of 32.5%. Stringent in solium kanamycin screening of the treated plants in T 1 and T 2 generations yielded 7 PCR positive tr...
Bruchid, a cosmopolitan and most notorious field carryover storage grain pest in greengram causes... more Bruchid, a cosmopolitan and most notorious field carryover storage grain pest in greengram causes severe damage in storage conditions. Hence, breeding efforts were made to develop bruchid tolerant genotypes involving two agronomically superior genotypes DGGV-2 and DGGV-7 and two bruchid tolerant genotypes V-02-709 and V-02-802. The F3 genotypes were screened through a ‘free choice test’ and further evaluated under the ‘force choice test’ for confirmation of resistance. The genotypes derived from the F3 family involving cross between V-02-802 × DGGV-7 and V-02-802 × DGGV-2 were found to be resistant with a longer mean developmental period (34.76 days and 33.53 days), low infestation (27.66 and 30.33 %) and low susceptibility index (0.039 and 0.043). The correlation amongst the various parameters, the mean developmental period has shown significantly negative correlation (r = -0.848) with susceptibility index; indicating that the resistance genotypes have shown a delay in a developmen...
Indian Journal of Genetics and Plant Breeding, 1994
One hundred and ninety six germplasm accessions of sunflower were evaluated for yield and yield c... more One hundred and ninety six germplasm accessions of sunflower were evaluated for yield and yield components to study the extent of variation in different quantitative traits. The germplasm accessions were also characterized on the basis of qualitative traits. Highest phenotypic and genotypic coefficients of variation were recorded for 100-seed weight, followed by seed yield, oil yield, number of filled seeds per plant, plant height and head diameter. Heritability was highest for 100-seed weight (72.0%), followed by seed (68.6%), number of filled seeds per plant (64.9%), oil yield (64.1%), and oil content (56.0%). Genetic advanceas percent of mean was highest for oil yield (50.7%), followed by seed yield (50.6%) and number of filled seeds per plant (33.9%). Qualitative traits also showed wide variability among the genotypes.
One hundred ninety six germplasm accessions of sunflower were included in this study which reveal... more One hundred ninety six germplasm accessions of sunflower were included in this study which revealed that seed yield had highest correlation with number of filled seeds per plant, followed by seed filling percentage and head diameter. Oil yield was highly and positively correlated with seed yield per plant and number of filled seeds per plant. Number of filled seeds per plant had maximum direct effect on seed yield. Head diameter also had considerable effect on seed yield. The present study brings out the importance of seeds per plant, head diameter and 1000-seed weight for yield improvement.
Abstract: Simple Sequence Repeat (SSR) DNA markers or Microsatellites are di-, tri-, tetra-nucleo... more Abstract: Simple Sequence Repeat (SSR) DNA markers or Microsatellites are di-, tri-, tetra-nucleotide tandem repeats containing loci of eukaryotic genomes. It is demonstrated that these loci are very polymorphic due to the change in the number of repeating units among the individuals of ...
Background: Despite the best efforts for improving the greengram varieties, the yield potential o... more Background: Despite the best efforts for improving the greengram varieties, the yield potential of this crop remains low owing to both biotic and abiotic factors. Yield losses caused due to various diseases were estimated to be 24-67 per cent in anthracnose, 80-100 per cent in mungbean yellow mosaic virus and 40 per cent due to powdery mildew So, the use of resistant cultivars is the most effective and eco-friendly method of managing the disease. Methods: A series of field experiments spanned over seven years (2014-2021) were conducted at Main Agricultural Research Station, University of Agricultural Sciences, Dharwad, Karnataka. In the first set of experiments, the material comprised of eight varieties of greengram which were treated with 20kR gamma radiation. M1was planted in Kharif 2014 and each variety was bulk harvested. M2 was planted in summer 2014 and individual plant selection was carried out. Mutant families were advanced to the further generation in Kharif 2015 and Rabi 2...
The present investigation was carried out to understand the role of different genotypes with its ... more The present investigation was carried out to understand the role of different genotypes with its surrounding environments interaction in the expression of various characters and stability over successive seasons. The phenotypic stability of genotypes were estimated by mean performance over seasons, the regression coefficient (bi) and deviation from regression. Based on stability parameters the genotypes DGGV-25 and DGGV-40 exhibited moderate mean performance for seeds yield per plant with regression value closer to unity and non-significant deviation from regression, indicating high stability and wider adaptability across three environments. These genotypes also confer Moderate Resistance to MYMV and their performance could be checked under Multi location trials, which may also serve as better source for MYMV resistance..
Background: Mungbean yellow mosaic virus disease is the most devastating disease on Mungbean prod... more Background: Mungbean yellow mosaic virus disease is the most devastating disease on Mungbean production. The virus is transmitted by whitefly and can cause yield losses from 75 to 100 per cent. The development of mungbean cultivars resistant to both virus and its vector is considered as one of the most desirable means of managing the disease as it is environmentally safe and highly efficient. The selection of resistant genotypes in conventional methods is complex and time consuming. Hence, the use of molecular markers linked with resistance genes is powerful as it hastens the breeding programmes. The current study was aimed to develop mapping population and to validate molecular markers associated with Mungbean yellow mosaic virus (MYMV). Methods: The present investigation was carried out with 260 F2 individuals that were derived from crossing DGGV-2 and IPM 2-14 during Kharif-2017 at Main Agril Research Station, UAS, Dharwad. Hybrid seeds of this cross were harvested individually a...
Genetically engineered resistance has been actively investigated in recent years as an alternativ... more Genetically engineered resistance has been actively investigated in recent years as an alternative for genes from wild relatives. The development of a method to obtain transformants, which is independent of problems inherent to tissue culture procedures, has been the ultimate goal of plant breeders. The alternative method, which involves Agrobacterium, but does not require tissue culture steps, is in planta tansformation. Although use of pollen tube pathway to deliver foreign DNA in to embryo sacs first reported in cotton was encouraging the overall quality of the data was very weak. Therefore, our attempts to modify this method yielded fruitful results. Out of 5 619 flowers, which were treated with Agrobacterium, where in the stigmatic surface was treated with 5% sucrose along with 20 ppm boric acid prior to application of Agrobacterium resulted in a boll set of 32.5%. Stringent in solium kanamycin screening of the treated plants in T 1 and T 2 generations yielded 7 PCR positive tr...
Bruchid, a cosmopolitan and most notorious field carryover storage grain pest in greengram causes... more Bruchid, a cosmopolitan and most notorious field carryover storage grain pest in greengram causes severe damage in storage conditions. Hence, breeding efforts were made to develop bruchid tolerant genotypes involving two agronomically superior genotypes DGGV-2 and DGGV-7 and two bruchid tolerant genotypes V-02-709 and V-02-802. The F3 genotypes were screened through a ‘free choice test’ and further evaluated under the ‘force choice test’ for confirmation of resistance. The genotypes derived from the F3 family involving cross between V-02-802 × DGGV-7 and V-02-802 × DGGV-2 were found to be resistant with a longer mean developmental period (34.76 days and 33.53 days), low infestation (27.66 and 30.33 %) and low susceptibility index (0.039 and 0.043). The correlation amongst the various parameters, the mean developmental period has shown significantly negative correlation (r = -0.848) with susceptibility index; indicating that the resistance genotypes have shown a delay in a developmen...
Indian Journal of Genetics and Plant Breeding, 1994
One hundred and ninety six germplasm accessions of sunflower were evaluated for yield and yield c... more One hundred and ninety six germplasm accessions of sunflower were evaluated for yield and yield components to study the extent of variation in different quantitative traits. The germplasm accessions were also characterized on the basis of qualitative traits. Highest phenotypic and genotypic coefficients of variation were recorded for 100-seed weight, followed by seed yield, oil yield, number of filled seeds per plant, plant height and head diameter. Heritability was highest for 100-seed weight (72.0%), followed by seed (68.6%), number of filled seeds per plant (64.9%), oil yield (64.1%), and oil content (56.0%). Genetic advanceas percent of mean was highest for oil yield (50.7%), followed by seed yield (50.6%) and number of filled seeds per plant (33.9%). Qualitative traits also showed wide variability among the genotypes.
One hundred ninety six germplasm accessions of sunflower were included in this study which reveal... more One hundred ninety six germplasm accessions of sunflower were included in this study which revealed that seed yield had highest correlation with number of filled seeds per plant, followed by seed filling percentage and head diameter. Oil yield was highly and positively correlated with seed yield per plant and number of filled seeds per plant. Number of filled seeds per plant had maximum direct effect on seed yield. Head diameter also had considerable effect on seed yield. The present study brings out the importance of seeds per plant, head diameter and 1000-seed weight for yield improvement.
Abstract: Simple Sequence Repeat (SSR) DNA markers or Microsatellites are di-, tri-, tetra-nucleo... more Abstract: Simple Sequence Repeat (SSR) DNA markers or Microsatellites are di-, tri-, tetra-nucleotide tandem repeats containing loci of eukaryotic genomes. It is demonstrated that these loci are very polymorphic due to the change in the number of repeating units among the individuals of ...
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