SummaryFive Phytophthora species were isolated from the rhizosphere of ash‐dominated areas of the... more SummaryFive Phytophthora species were isolated from the rhizosphere of ash‐dominated areas of the Białowieza European lowland forest nature reserve area. Morphological and DNA analyses identified Phytophthora fragariaefolia, P. plurivora, P. cactorum, P. lacustris and P. pseudosyringae. This is the first record of P. fragariaefolia in ash forests and demonstrates that several species in the potentially highly damaging Phytophthora genus are present within a near‐pristine ecosystem.
Agriculture can degrade soils and reduce microbial diversity. The reduction in microbial diversit... more Agriculture can degrade soils and reduce microbial diversity. The reduction in microbial diversity of degraded soils is due to their long-term agricultural use. In most cases, such areas are afforested but rarely succeed in converting them into first-generation pine forests without adequately revitalizing the soils and restoring the natural relationships characteristic of forest habitats. This is possible thanks to the positive changes in soil biodiversity. To facilitate and enhance this phenomenon in the present experiment, various forms of organic matter were added to the soil: pine bark compost scattered on the soil surface (BCS) or placed under the roots of the seedlings at planting (BCR), woody debris (WW) and sawdust (S), while the control plot was not treated. The studies started in 2001 on two experimental plots, a poorer one in Bielsk and a more fertile one in Czarne Człuchowskie (eastern and northern Poland, respectively). The following year, 2-year-old pine seedlings (Pin...
In the construction of electronic nose devices, two groups of measurement setups could be disting... more In the construction of electronic nose devices, two groups of measurement setups could be distinguished when we take into account the design of electronic nose chambers. The simpler one consists of placing the sensors directly in the environment of the measured gas, which has an important advantage, in that the composition of the gas is not changed as the gas is not diluted. However, that has an important drawback in that it is difficult to clean sensors between measurement cycles. The second, more advanced construction, contains a pneumatic system transporting the gas inside a specially designed sensor chamber. A new design of an electronic nose gas sensor chamber is proposed, which consists of a sensor chamber with a sliding chamber shutter, equipped with a simple pneumatic system for cleaning the air. The proposal combines the advantages of both approaches to the sensor chamber designs. The sensors can be effectively cleared by the flow of clean air, while the measurements are pe...
The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 wer... more The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 were shown to be potent biocontrol agents active against several plant diseases in agricultural and forest ecosystems. Both strains produce the cyclic peptide gramicidin S (GS) that was described as the main weapon inhibiting some gram-negative and gram-positive bacteria and fungus-like organisms along with the production of biosurfactant and hemolysis activities. However, the contribution of the cyclic peptide gramicidin S (GS) to the biocontrol ability of A. migulanus has never been studied experimentally. In this paper, using a mutant of the A. migulanus Nagano strain (E1 mutant) impaired in GS biosynthesis we evaluated the contribution of GS in the biocontrol potential of A. migulanus against Phytophthora spp. The two strains of A. migulanus, Nagano and NCTC 7096, were tested in a pilot study for the inhibition of the growth of 13 Phytophthora species in dual culture assays. A. migulanu...
Wood technology is constantly looking for environmentally friendly technological solutions. It se... more Wood technology is constantly looking for environmentally friendly technological solutions. It seems that nanotechnology can provide green and environmentally friendly alternatives for wood protection. In this study, the antifungal activity of silver and copper nanoparticles against the white rot fungus Fomes fomentarius was investigated under in vitro conditions and with respect to the protection of Fagus sylvatica wood. In both experiments, 5, 25 and 50 ppm of nanoparticles were used. The results of the in vitro test showed selective antifungal activity of the nanoparticles. All concentrations of copper nanoparticles stimulated mycelial growth. The stimulation was inversely proportional to the concentration. Silver nanoparticles inhibited fungal colony growth at the highest concentration (50 ppm) and did not affect growth at concentrations of 5 and 25 ppm. Silver nanoparticles increased the rot resistance of beech wood, but only at the highest concentration. Decay caused by F. fom...
The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 wer... more The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 were shown to be potent biocontrol agents active against several plant diseases in agricultural and forest ecosystems. Both strains produce the cyclic peptide gramicidin S (GS) that was described as the main weapon inhibiting some gram-negative and gram-positive bacteria and fungus-like organisms along with the production of biosurfactant and hemolysis activities. However, the contribution of the cyclic peptide gramicidin S (GS) to the biocontrol ability of A. migulanus has never been studied experimentally. In this paper, using a mutant of the A. migulanus Nagano strain (E1 mutant) impaired in GS biosynthesis we evaluated the contribution of GS in the biocontrol potential of A. migulanus against Phytophthora spp. The two strains of A. migulanus, Nagano and NCTC 7096, were tested in a pilot study for the inhibition of the growth of 13 Phytophthora species in dual culture assays. A. migulanu...
Wood technology is constantly looking for environmentally friendly technological solutions. It se... more Wood technology is constantly looking for environmentally friendly technological solutions. It seems that nanotechnology can provide green and environmentally friendly alternatives for wood protection. In this study, the antifungal activity of silver and copper nanoparticles against the white rot fungus Fomes fomentarius was investigated under in vitro conditions and with respect to the protection of Fagus sylvatica wood. In both experiments, 5, 25 and 50 ppm of nanoparticles were used. The results of the in vitro test showed selective antifungal activity of the nanoparticles. All concentrations of copper nanoparticles stimulated mycelial growth. The stimulation was inversely proportional to the concentration. Silver nanoparticles inhibited fungal colony growth at the highest concentration (50 ppm) and did not affect growth at concentrations of 5 and 25 ppm. Silver nanoparticles increased the rot resistance of beech wood, but only at the highest concentration. Decay caused by F. fom...
W niniejszym opracowaniu chodzi o zastosowanie pojęcia "paradoks" do opisu zjawisk char... more W niniejszym opracowaniu chodzi o zastosowanie pojęcia "paradoks" do opisu zjawisk charakterystycznych dla cywilizowanej ludzkości. Ujawniają się one w trakcie realizacji nowożytnego programu naukowo-technicznego panowania nad przyrodą. Z jednej strony umożliwia on realizację nowożytnych celów człowieka, tj. przezwyciężania biedy materialnej i chorób, z drugiej zaś strony równocześnie prowadzi do zagrożeń środowiskowych podstaw ludzkiej egzystencji. Opracowanie to stanowi próbę wskazania źródeł i przejawów tych zjawisk oraz wyznaczenia perspektyw ich opanowania i przezwyciężenia. Efektem tej próby jest wskazanie na antropologiczny charakter źródeł paradoksów ekologicznych. Sięgają one samej natury człowieka i wyrażają się w trzech aspektach: biologicznym, psychologicznym i umysłowym/kulturowym.<br> W aspekcie biologicznym chodzi o właściwe człowiekowi obecnemu w przyrodzie działania nastawione na "sukces ad hoc". W aspekcie psychologicznym chodzi o rozpozna...
Fusarium circinatum Nirenberg & O’Donnel is listed among the species recommended for regulation a... more Fusarium circinatum Nirenberg & O’Donnel is listed among the species recommended for regulation as quarantine pests in Europe. Over 60 Pinus species are susceptible to the pathogen and it also causes disease on Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and species in genera such as Picea and Larix. The European Food Safety Authority considers the probability of new introductions—via contaminated seeds, wood material, soil and growing substrates, natural means and human activities—into the EU very likely. Due to early detection, constant surveillance and control measures, F. circinatum outbreaks have officially been eradicated in Italy and France. However, the global spread of F. circinatum suggests that the pathogen will continue to be encountered in new environments in the future. Therefore, continuous surveillance of reproductive material, nurseries and plantations, prompt control measures and realistic contingency plans will be important in Europe and elsewhere to limit ...
The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and plant... more The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and planted stands of sweet chestnut in Serbia. The fungus was detected on the sweet chestnut in five localities and on the sessile oak in one locality. In total, 77 isolates from the sweet chestnut and five isolates from the sessile oak were obtained. Based on the culture morphology, all the obtained isolates were proven to be free from Cryphonectria (Saccardo) Saccardo & D. Saccardo hypovirus. The isolates of C. parasitica from the sweet chestnut were compatible with three different vegetative compatibility types, EU-12, EU-2, and EU-1, while the isolates from the sessile oak belonged to EU-12. After inoculation in laboratory conditions, the isolate from the sweet chestnut and sessile oak caused the decline in 88 and 76% of the sweet chestnut plants, respectively. In the case of the sessile oak, both isolates caused the decline in 52% of the plants. In field conditions, both isolates were aggre...
The complex phenomenon of decline in European oak is currently triggered by changing climatic con... more The complex phenomenon of decline in European oak is currently triggered by changing climatic conditions and their consequences like heavy rains, local floods and pest development. Especially, pathogens from Phytophthora genus profit from soil saturation with water. They are alien invasive species, which attack and severely damage fine roots. In drought conditions occurring in the subsequent year, many oaks die as they encounter problem with water uptake. Additionally, insect defoliators followed by oak mildew infections accelerate the level of tree mortality. Secondary insects, butt and root pathogens are usually the final cause of death of many oaks. More research is needed in the direction to determine (i) measurable factors (e.g. chlorophyll florescence) that can indicate that the process of tree decline has already started, (ii) the correlation between the root decay and the crown symptoms (scanners, software), (iii) which combination of stressors stimulate the best development...
Our aim was to examine the virulence of eight Phytophthora isolates belonging to three species (P... more Our aim was to examine the virulence of eight Phytophthora isolates belonging to three species (Phytophthora cryptogea, Phytophthora plurivora and Phytophthora quercina) obtained from diverse European ecosystems (in Bulgaria, Poland and Germany) towards three forest tree hosts – English oak (Quercus robur L.), Turkey oak (Quercus cerris L.) and European beech (Fagus sylvatica L.). All plants grown from seeds in a greenhouse conditions were artificially inoculated under the stem bark with Phytophthora cultures. The tested isolates turned to be more aggressive to Turkey oaks than to English oak trees. In case of European beech, the isolates of P. cryptogea and P. plurivora exposed various virulence. The potential hazard of the introduced foreign isolates for the oak and beech forests in Poland and Bulgaria is discussed. Amongst the tested isolates, P. quercina P290 from German highly infected Bulgarian Turkey oaks; therefore, its negative potential impact on Bulgarian oak forests coul...
SummaryFive Phytophthora species were isolated from the rhizosphere of ash‐dominated areas of the... more SummaryFive Phytophthora species were isolated from the rhizosphere of ash‐dominated areas of the Białowieza European lowland forest nature reserve area. Morphological and DNA analyses identified Phytophthora fragariaefolia, P. plurivora, P. cactorum, P. lacustris and P. pseudosyringae. This is the first record of P. fragariaefolia in ash forests and demonstrates that several species in the potentially highly damaging Phytophthora genus are present within a near‐pristine ecosystem.
Agriculture can degrade soils and reduce microbial diversity. The reduction in microbial diversit... more Agriculture can degrade soils and reduce microbial diversity. The reduction in microbial diversity of degraded soils is due to their long-term agricultural use. In most cases, such areas are afforested but rarely succeed in converting them into first-generation pine forests without adequately revitalizing the soils and restoring the natural relationships characteristic of forest habitats. This is possible thanks to the positive changes in soil biodiversity. To facilitate and enhance this phenomenon in the present experiment, various forms of organic matter were added to the soil: pine bark compost scattered on the soil surface (BCS) or placed under the roots of the seedlings at planting (BCR), woody debris (WW) and sawdust (S), while the control plot was not treated. The studies started in 2001 on two experimental plots, a poorer one in Bielsk and a more fertile one in Czarne Człuchowskie (eastern and northern Poland, respectively). The following year, 2-year-old pine seedlings (Pin...
In the construction of electronic nose devices, two groups of measurement setups could be disting... more In the construction of electronic nose devices, two groups of measurement setups could be distinguished when we take into account the design of electronic nose chambers. The simpler one consists of placing the sensors directly in the environment of the measured gas, which has an important advantage, in that the composition of the gas is not changed as the gas is not diluted. However, that has an important drawback in that it is difficult to clean sensors between measurement cycles. The second, more advanced construction, contains a pneumatic system transporting the gas inside a specially designed sensor chamber. A new design of an electronic nose gas sensor chamber is proposed, which consists of a sensor chamber with a sliding chamber shutter, equipped with a simple pneumatic system for cleaning the air. The proposal combines the advantages of both approaches to the sensor chamber designs. The sensors can be effectively cleared by the flow of clean air, while the measurements are pe...
The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 wer... more The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 were shown to be potent biocontrol agents active against several plant diseases in agricultural and forest ecosystems. Both strains produce the cyclic peptide gramicidin S (GS) that was described as the main weapon inhibiting some gram-negative and gram-positive bacteria and fungus-like organisms along with the production of biosurfactant and hemolysis activities. However, the contribution of the cyclic peptide gramicidin S (GS) to the biocontrol ability of A. migulanus has never been studied experimentally. In this paper, using a mutant of the A. migulanus Nagano strain (E1 mutant) impaired in GS biosynthesis we evaluated the contribution of GS in the biocontrol potential of A. migulanus against Phytophthora spp. The two strains of A. migulanus, Nagano and NCTC 7096, were tested in a pilot study for the inhibition of the growth of 13 Phytophthora species in dual culture assays. A. migulanu...
Wood technology is constantly looking for environmentally friendly technological solutions. It se... more Wood technology is constantly looking for environmentally friendly technological solutions. It seems that nanotechnology can provide green and environmentally friendly alternatives for wood protection. In this study, the antifungal activity of silver and copper nanoparticles against the white rot fungus Fomes fomentarius was investigated under in vitro conditions and with respect to the protection of Fagus sylvatica wood. In both experiments, 5, 25 and 50 ppm of nanoparticles were used. The results of the in vitro test showed selective antifungal activity of the nanoparticles. All concentrations of copper nanoparticles stimulated mycelial growth. The stimulation was inversely proportional to the concentration. Silver nanoparticles inhibited fungal colony growth at the highest concentration (50 ppm) and did not affect growth at concentrations of 5 and 25 ppm. Silver nanoparticles increased the rot resistance of beech wood, but only at the highest concentration. Decay caused by F. fom...
The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 wer... more The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 were shown to be potent biocontrol agents active against several plant diseases in agricultural and forest ecosystems. Both strains produce the cyclic peptide gramicidin S (GS) that was described as the main weapon inhibiting some gram-negative and gram-positive bacteria and fungus-like organisms along with the production of biosurfactant and hemolysis activities. However, the contribution of the cyclic peptide gramicidin S (GS) to the biocontrol ability of A. migulanus has never been studied experimentally. In this paper, using a mutant of the A. migulanus Nagano strain (E1 mutant) impaired in GS biosynthesis we evaluated the contribution of GS in the biocontrol potential of A. migulanus against Phytophthora spp. The two strains of A. migulanus, Nagano and NCTC 7096, were tested in a pilot study for the inhibition of the growth of 13 Phytophthora species in dual culture assays. A. migulanu...
Wood technology is constantly looking for environmentally friendly technological solutions. It se... more Wood technology is constantly looking for environmentally friendly technological solutions. It seems that nanotechnology can provide green and environmentally friendly alternatives for wood protection. In this study, the antifungal activity of silver and copper nanoparticles against the white rot fungus Fomes fomentarius was investigated under in vitro conditions and with respect to the protection of Fagus sylvatica wood. In both experiments, 5, 25 and 50 ppm of nanoparticles were used. The results of the in vitro test showed selective antifungal activity of the nanoparticles. All concentrations of copper nanoparticles stimulated mycelial growth. The stimulation was inversely proportional to the concentration. Silver nanoparticles inhibited fungal colony growth at the highest concentration (50 ppm) and did not affect growth at concentrations of 5 and 25 ppm. Silver nanoparticles increased the rot resistance of beech wood, but only at the highest concentration. Decay caused by F. fom...
W niniejszym opracowaniu chodzi o zastosowanie pojęcia "paradoks" do opisu zjawisk char... more W niniejszym opracowaniu chodzi o zastosowanie pojęcia "paradoks" do opisu zjawisk charakterystycznych dla cywilizowanej ludzkości. Ujawniają się one w trakcie realizacji nowożytnego programu naukowo-technicznego panowania nad przyrodą. Z jednej strony umożliwia on realizację nowożytnych celów człowieka, tj. przezwyciężania biedy materialnej i chorób, z drugiej zaś strony równocześnie prowadzi do zagrożeń środowiskowych podstaw ludzkiej egzystencji. Opracowanie to stanowi próbę wskazania źródeł i przejawów tych zjawisk oraz wyznaczenia perspektyw ich opanowania i przezwyciężenia. Efektem tej próby jest wskazanie na antropologiczny charakter źródeł paradoksów ekologicznych. Sięgają one samej natury człowieka i wyrażają się w trzech aspektach: biologicznym, psychologicznym i umysłowym/kulturowym.<br> W aspekcie biologicznym chodzi o właściwe człowiekowi obecnemu w przyrodzie działania nastawione na "sukces ad hoc". W aspekcie psychologicznym chodzi o rozpozna...
Fusarium circinatum Nirenberg & O’Donnel is listed among the species recommended for regulation a... more Fusarium circinatum Nirenberg & O’Donnel is listed among the species recommended for regulation as quarantine pests in Europe. Over 60 Pinus species are susceptible to the pathogen and it also causes disease on Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and species in genera such as Picea and Larix. The European Food Safety Authority considers the probability of new introductions—via contaminated seeds, wood material, soil and growing substrates, natural means and human activities—into the EU very likely. Due to early detection, constant surveillance and control measures, F. circinatum outbreaks have officially been eradicated in Italy and France. However, the global spread of F. circinatum suggests that the pathogen will continue to be encountered in new environments in the future. Therefore, continuous surveillance of reproductive material, nurseries and plantations, prompt control measures and realistic contingency plans will be important in Europe and elsewhere to limit ...
The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and plant... more The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and planted stands of sweet chestnut in Serbia. The fungus was detected on the sweet chestnut in five localities and on the sessile oak in one locality. In total, 77 isolates from the sweet chestnut and five isolates from the sessile oak were obtained. Based on the culture morphology, all the obtained isolates were proven to be free from Cryphonectria (Saccardo) Saccardo & D. Saccardo hypovirus. The isolates of C. parasitica from the sweet chestnut were compatible with three different vegetative compatibility types, EU-12, EU-2, and EU-1, while the isolates from the sessile oak belonged to EU-12. After inoculation in laboratory conditions, the isolate from the sweet chestnut and sessile oak caused the decline in 88 and 76% of the sweet chestnut plants, respectively. In the case of the sessile oak, both isolates caused the decline in 52% of the plants. In field conditions, both isolates were aggre...
The complex phenomenon of decline in European oak is currently triggered by changing climatic con... more The complex phenomenon of decline in European oak is currently triggered by changing climatic conditions and their consequences like heavy rains, local floods and pest development. Especially, pathogens from Phytophthora genus profit from soil saturation with water. They are alien invasive species, which attack and severely damage fine roots. In drought conditions occurring in the subsequent year, many oaks die as they encounter problem with water uptake. Additionally, insect defoliators followed by oak mildew infections accelerate the level of tree mortality. Secondary insects, butt and root pathogens are usually the final cause of death of many oaks. More research is needed in the direction to determine (i) measurable factors (e.g. chlorophyll florescence) that can indicate that the process of tree decline has already started, (ii) the correlation between the root decay and the crown symptoms (scanners, software), (iii) which combination of stressors stimulate the best development...
Our aim was to examine the virulence of eight Phytophthora isolates belonging to three species (P... more Our aim was to examine the virulence of eight Phytophthora isolates belonging to three species (Phytophthora cryptogea, Phytophthora plurivora and Phytophthora quercina) obtained from diverse European ecosystems (in Bulgaria, Poland and Germany) towards three forest tree hosts – English oak (Quercus robur L.), Turkey oak (Quercus cerris L.) and European beech (Fagus sylvatica L.). All plants grown from seeds in a greenhouse conditions were artificially inoculated under the stem bark with Phytophthora cultures. The tested isolates turned to be more aggressive to Turkey oaks than to English oak trees. In case of European beech, the isolates of P. cryptogea and P. plurivora exposed various virulence. The potential hazard of the introduced foreign isolates for the oak and beech forests in Poland and Bulgaria is discussed. Amongst the tested isolates, P. quercina P290 from German highly infected Bulgarian Turkey oaks; therefore, its negative potential impact on Bulgarian oak forests coul...
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