The relative DNA content was studied in seven species of the genus Spiraea L., section Chamaedryo... more The relative DNA content was studied in seven species of the genus Spiraea L., section Chamaedryon Ser., and in two species, section Glomerati Nakai, from 28 natural populations growing in Asian Russia. The cell nuclei were isolated from a leaf tissue. The relative intensity of fluorescence was measured using flow cytometry of propidium iodide-stained nuclei. The analysis was performed using a CyFlowSpace device (Germany, Sysmex Partec) with a laser radiation source of 532 nm. Fresh leaves of Solanum lycopersicum cv. ‘Stupice’ were used as an internal standard. Data on the relative DNA content are presented for the first time for S. flexuosa Fisch ex Cambess. (0.42–0.47 pg), S. ussuriensis Pojark. (0.49–0.52; 0.85 pg), S. alpina-Pall. (0.49–0.51 pg), S. media Schmidt. (0.45; 0.98–1.01 pg), S. trilobata L. (0.46 pg), S. hypericifolia L. (0.49–0.52 pg) and S. aquilegifolia Pall. (0.48–0.51 pg). Mesophytic species of the genus Spiraea growing in the forest zone (S. chamaedryfolia L. an...
In 1965 T. I. Isachenko made a scheme of geobotanical zoning for the southern part of the Amur-Ze... more In 1965 T. I. Isachenko made a scheme of geobotanical zoning for the southern part of the Amur-Zeya interfluve. She identified five geobotanical districts and subdistricts within the boundaries of zones and subzones. This zoning stays relevant to the present day. V. V. Lipatova (1969а) created the large-scale geobotanical maps of key areas for the Amur and Central districts. No other work on vegetation mapping in the Amur-Zeya interfluve was done for this area. The research goal is to make a large-scale vegetation map of the study area showing the current state and diversity of the vegetation in the Prizeya geobotanical district (Isachenko, 1965). The study area is located in the eastern part of the Amur-Zeya interfluve, subtaiga (broad-leaved-coniferous) subzone. This area demonstrates the most industrial and agricultural development of mentioned above territory (Fig. 1). Vegetation map at a scale of 1 : 25 000 is based on field data (236 geobotanical descriptions); interpretation of Sentinel-2 satellite images was made. The classification scheme of the natural vegetation cover according to the ecological-phytocoenotic (dominant) approach was created. Mapping units are represented by groups of associations. The titles of the legend are the subzonal plant communities. Vegetation types (forest, shrub, meadow) and groups of vegetation types (mires, aquatic and coastal, meadow-shrub) are presented in the subtitles. Forests are divided into classes of formations (light coniferous, coniferous-broad-leaved and coniferous-small-leaved, broad-leaved, broad-small-leaved, small-leaved) and disturbed communities of burned areas (sparse and young forests). Transformed vegetation has been identified in the disturbed territory. The main classification criterion is the type of anthropogenic impact. The map legend contains 45 names with color and hatching (Fig. 4). Equity participation of plant communities of the study area was presented. Disturbed territories occupy large areas of 7946 hectares (27.8 %). The forested area covers 29.7 %, including small-leaved forests (7.2 %) and sparse and young forests 8.0 %). Pine forests and their derivatives pine-oak, oak-pine, birch-pine and pine-birch forests occupy 2.8 %, oak forests — 2.5 %, the broad-small-leaved forests — 3.7 %. A significant area is occupied by meadow vegetation — 22.4 %, mire vegetation accounts for 6.7 %, dwarf birch (yernik) and willows — 4.9 %, aquatic and coastal communities — 1.4 % (Table). The vegetation map shows the main features of vegetation cover of the Prizeya geobotanical district and allows to give a correct botanical-geographic interpretation of the territory.
At least 22 phenolic compounds were identified in aqueous-ethanol extracts from Sorbaria pallasii... more At least 22 phenolic compounds were identified in aqueous-ethanol extracts from Sorbaria pallasii (G. Don fil.) Pojark. leaves and at least 28 in its inflorescences by High-Performance Liquid Chromatography. Two acids (chlorogenic and p-hydroxybenzoic) and five flavonols (hyperoside, isoquercitrin, quercitrin, kaempferol, and astragalin) were identified among them. Flavonoid aglycones were obtained from the leaves and inflorescences by hydrochloric-acid hydrolysis of the aqueous-ethanol extracts (1:1). Three flavonol aglycones (quercetin, kaempferol, and isorhamnetin) were identified in the extract hydrolysates from S. pallasii above-ground organs through chromatographic analysis. Additionally, a flavone aglycone (luteolin) was found in the extract hydrolysates from the leaves. Kaempferol glycosides are predominant phenolic compounds in S. pallasii inflorescences, and quercetin glycosides in its leaves.
Taxonomy: This study was supported by Agencia Nacional de Promoción Científica y Técnica (ANPCyT)... more Taxonomy: This study was supported by Agencia Nacional de Promoción Científica y Técnica (ANPCyT) grant no. PICT-2017-4203 and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), and a postdoctoral fellowship from CONICET to AVR.Fil: Marhold, Karol. Slovak Academy of Sciences. Institute of Botany; Eslovaquia. Karlova Univerzita (cuni); República ChecaFil: Kucera, Jaromír. Slovak Academy of Sciences. Institute of Botany; EslovaquiaFil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Akopian, Janna A.. Armenian National Academy of Sciences; ArmeniaFil: de Almeida, Erton M.. Universidade Federal de Pernambuco; Brasil. Universidade Federal da Paraíba; BrasilFil: Alves, Marccus V.. Universidade Federal da Paraíba; BrasilFil: Amorim, Bruno. Museu da Amazônia; Brasil. Universidade do Estado do Amazona; BrasilFil: An'kova, Tatyana V.. Academia de Ciencias de Rusia; RusiaFil: Arora, Jaya. University of Delhi; IndiaFil: Aytaç, Zeki. Gazi Üniversitesi; TurquíaFil: Baez, Jesica Mariana. Universidade Federal de Pernambuco; Brasil. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cavalcanti, Taciana Barbosa. Parque Estação Biológica; BrasilFil: Calvente, Alice. Universidade de Sao Paulo; Brasil. Universidade Federal do Rio Grande do Norte; BrasilFil: Catalan, Pilar. Tomsk State University; Rusia. Universidad de Zaragoza; EspañaFil: Chernyagina, Olga A.. Academia de Ciencias de Rusia; RusiaFil: Chernysheva, Olga A.. Academia de Ciencias de Rusia; RusiaFil: Cordeiro, Joel M. P.. Universidade Estadual da Paraiba; BrasilFil: Daviña, Julio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Deanna, Rocío. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. State University of Colorado at Boulder; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Delgado, Luis. Universidad de Salamanca; EspañaFil: Dias Silva, Yhanndra K.. Universidade Federal de Pernambuco; BrasilFil: Elliott, Tammy L.. University of Cape Town; Sudáfrica. University of Montreal; CanadáFil: Erst, Andrey S.. Tomsk State University; Rusia. Academia de Ciencias de Rusia; RusiaFil: Felix, Leonardo P.. Universidade Federal da Paraíba; BrasilFil: Forni Martins, Eliana R.. Universidade Estadual de Campinas; BrasilFil: Gallego, Francisca. Universidad de Salamanca; EspañaFil: Facco, Marlon Garlet. Universidade de Brasília; BrasilFil: Gianini Aquino, Analía Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Gomes de Andrade, Maria J.. Universidade do Estado da Bahia; BrasilFil: Rua, Gabriel Hugo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Botánica Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
The leaves of seventeen cultivars of olive growing in the north of Iran were investigated for tot... more The leaves of seventeen cultivars of olive growing in the north of Iran were investigated for total phenol content and antioxidant activity. The identification and quantification of main phenolic compounds were performed by reverse phase high performance liquid chromatography with diode array detector. The cultivars Kalamon, Gordal, and Coratina contained the highest concentration of phenolic compounds (190.65 ± 0.03, 184.72 ± 0.001, and 155.91 ± 0.06 mg GAE/g extract, respectively). The maximum radical scavenging activities were found in Gordal, Coratina, and Kalamon extracts (IC 20.66, 22.95, and 26.74 µg ml, respectively). The extracts of Mishen, Fishomi, and Arbequina (1971.37 ± 0.007, 1794.57 ± 0.001, and 1760.57 ± 0.005 µmol Fe II/g dried extract, respectively) showed highest antioxidant activity in FRAP assay. The identification analysis demonstrated the present of vanillin, rutin, luteolin 7-O-glucoside, oleuropein, and quercetin. The highest oleuropein concentrations were d...
The relative DNA content was studied in seven species of the genus Spiraea L., section Chamaedryo... more The relative DNA content was studied in seven species of the genus Spiraea L., section Chamaedryon Ser., and in two species, section Glomerati Nakai, from 28 natural populations growing in Asian Russia. The cell nuclei were isolated from a leaf tissue. The relative intensity of fluorescence was measured using flow cytometry of propidium iodide-stained nuclei. The analysis was performed using a CyFlowSpace device (Germany, Sysmex Partec) with a laser radiation source of 532 nm. Fresh leaves of Solanum lycopersicum cv. ‘Stupice’ were used as an internal standard. Data on the relative DNA content are presented for the first time for S. flexuosa Fisch ex Cambess. (0.42–0.47 pg), S. ussuriensis Pojark. (0.49–0.52; 0.85 pg), S. alpina-Pall. (0.49–0.51 pg), S. media Schmidt. (0.45; 0.98–1.01 pg), S. trilobata L. (0.46 pg), S. hypericifolia L. (0.49–0.52 pg) and S. aquilegifolia Pall. (0.48–0.51 pg). Mesophytic species of the genus Spiraea growing in the forest zone (S. chamaedryfolia L. an...
In 1965 T. I. Isachenko made a scheme of geobotanical zoning for the southern part of the Amur-Ze... more In 1965 T. I. Isachenko made a scheme of geobotanical zoning for the southern part of the Amur-Zeya interfluve. She identified five geobotanical districts and subdistricts within the boundaries of zones and subzones. This zoning stays relevant to the present day. V. V. Lipatova (1969а) created the large-scale geobotanical maps of key areas for the Amur and Central districts. No other work on vegetation mapping in the Amur-Zeya interfluve was done for this area. The research goal is to make a large-scale vegetation map of the study area showing the current state and diversity of the vegetation in the Prizeya geobotanical district (Isachenko, 1965). The study area is located in the eastern part of the Amur-Zeya interfluve, subtaiga (broad-leaved-coniferous) subzone. This area demonstrates the most industrial and agricultural development of mentioned above territory (Fig. 1). Vegetation map at a scale of 1 : 25 000 is based on field data (236 geobotanical descriptions); interpretation of Sentinel-2 satellite images was made. The classification scheme of the natural vegetation cover according to the ecological-phytocoenotic (dominant) approach was created. Mapping units are represented by groups of associations. The titles of the legend are the subzonal plant communities. Vegetation types (forest, shrub, meadow) and groups of vegetation types (mires, aquatic and coastal, meadow-shrub) are presented in the subtitles. Forests are divided into classes of formations (light coniferous, coniferous-broad-leaved and coniferous-small-leaved, broad-leaved, broad-small-leaved, small-leaved) and disturbed communities of burned areas (sparse and young forests). Transformed vegetation has been identified in the disturbed territory. The main classification criterion is the type of anthropogenic impact. The map legend contains 45 names with color and hatching (Fig. 4). Equity participation of plant communities of the study area was presented. Disturbed territories occupy large areas of 7946 hectares (27.8 %). The forested area covers 29.7 %, including small-leaved forests (7.2 %) and sparse and young forests 8.0 %). Pine forests and their derivatives pine-oak, oak-pine, birch-pine and pine-birch forests occupy 2.8 %, oak forests — 2.5 %, the broad-small-leaved forests — 3.7 %. A significant area is occupied by meadow vegetation — 22.4 %, mire vegetation accounts for 6.7 %, dwarf birch (yernik) and willows — 4.9 %, aquatic and coastal communities — 1.4 % (Table). The vegetation map shows the main features of vegetation cover of the Prizeya geobotanical district and allows to give a correct botanical-geographic interpretation of the territory.
At least 22 phenolic compounds were identified in aqueous-ethanol extracts from Sorbaria pallasii... more At least 22 phenolic compounds were identified in aqueous-ethanol extracts from Sorbaria pallasii (G. Don fil.) Pojark. leaves and at least 28 in its inflorescences by High-Performance Liquid Chromatography. Two acids (chlorogenic and p-hydroxybenzoic) and five flavonols (hyperoside, isoquercitrin, quercitrin, kaempferol, and astragalin) were identified among them. Flavonoid aglycones were obtained from the leaves and inflorescences by hydrochloric-acid hydrolysis of the aqueous-ethanol extracts (1:1). Three flavonol aglycones (quercetin, kaempferol, and isorhamnetin) were identified in the extract hydrolysates from S. pallasii above-ground organs through chromatographic analysis. Additionally, a flavone aglycone (luteolin) was found in the extract hydrolysates from the leaves. Kaempferol glycosides are predominant phenolic compounds in S. pallasii inflorescences, and quercetin glycosides in its leaves.
Taxonomy: This study was supported by Agencia Nacional de Promoción Científica y Técnica (ANPCyT)... more Taxonomy: This study was supported by Agencia Nacional de Promoción Científica y Técnica (ANPCyT) grant no. PICT-2017-4203 and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), and a postdoctoral fellowship from CONICET to AVR.Fil: Marhold, Karol. Slovak Academy of Sciences. Institute of Botany; Eslovaquia. Karlova Univerzita (cuni); República ChecaFil: Kucera, Jaromír. Slovak Academy of Sciences. Institute of Botany; EslovaquiaFil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Akopian, Janna A.. Armenian National Academy of Sciences; ArmeniaFil: de Almeida, Erton M.. Universidade Federal de Pernambuco; Brasil. Universidade Federal da Paraíba; BrasilFil: Alves, Marccus V.. Universidade Federal da Paraíba; BrasilFil: Amorim, Bruno. Museu da Amazônia; Brasil. Universidade do Estado do Amazona; BrasilFil: An'kova, Tatyana V.. Academia de Ciencias de Rusia; RusiaFil: Arora, Jaya. University of Delhi; IndiaFil: Aytaç, Zeki. Gazi Üniversitesi; TurquíaFil: Baez, Jesica Mariana. Universidade Federal de Pernambuco; Brasil. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cavalcanti, Taciana Barbosa. Parque Estação Biológica; BrasilFil: Calvente, Alice. Universidade de Sao Paulo; Brasil. Universidade Federal do Rio Grande do Norte; BrasilFil: Catalan, Pilar. Tomsk State University; Rusia. Universidad de Zaragoza; EspañaFil: Chernyagina, Olga A.. Academia de Ciencias de Rusia; RusiaFil: Chernysheva, Olga A.. Academia de Ciencias de Rusia; RusiaFil: Cordeiro, Joel M. P.. Universidade Estadual da Paraiba; BrasilFil: Daviña, Julio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Deanna, Rocío. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. State University of Colorado at Boulder; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Delgado, Luis. Universidad de Salamanca; EspañaFil: Dias Silva, Yhanndra K.. Universidade Federal de Pernambuco; BrasilFil: Elliott, Tammy L.. University of Cape Town; Sudáfrica. University of Montreal; CanadáFil: Erst, Andrey S.. Tomsk State University; Rusia. Academia de Ciencias de Rusia; RusiaFil: Felix, Leonardo P.. Universidade Federal da Paraíba; BrasilFil: Forni Martins, Eliana R.. Universidade Estadual de Campinas; BrasilFil: Gallego, Francisca. Universidad de Salamanca; EspañaFil: Facco, Marlon Garlet. Universidade de Brasília; BrasilFil: Gianini Aquino, Analía Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Gomes de Andrade, Maria J.. Universidade do Estado da Bahia; BrasilFil: Rua, Gabriel Hugo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Botánica Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
The leaves of seventeen cultivars of olive growing in the north of Iran were investigated for tot... more The leaves of seventeen cultivars of olive growing in the north of Iran were investigated for total phenol content and antioxidant activity. The identification and quantification of main phenolic compounds were performed by reverse phase high performance liquid chromatography with diode array detector. The cultivars Kalamon, Gordal, and Coratina contained the highest concentration of phenolic compounds (190.65 ± 0.03, 184.72 ± 0.001, and 155.91 ± 0.06 mg GAE/g extract, respectively). The maximum radical scavenging activities were found in Gordal, Coratina, and Kalamon extracts (IC 20.66, 22.95, and 26.74 µg ml, respectively). The extracts of Mishen, Fishomi, and Arbequina (1971.37 ± 0.007, 1794.57 ± 0.001, and 1760.57 ± 0.005 µmol Fe II/g dried extract, respectively) showed highest antioxidant activity in FRAP assay. The identification analysis demonstrated the present of vanillin, rutin, luteolin 7-O-glucoside, oleuropein, and quercetin. The highest oleuropein concentrations were d...
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