Biliary strictures occur frequently after liver transplantation [1, 2]. Percutaneous management w... more Biliary strictures occur frequently after liver transplantation [1, 2]. Percutaneous management with benign stricture protocol is associated with high rates of long-term clinical patency [2–4]. Rarely, strictures can be severe and crossing with a catheter can be difficult or impossible. In such cases, sharp recanalization is a more aggressive alternative. We report a case of severe biliary stricturing where these traditional catheter crossing techniques failed yet the stricture was crossed easily with cholangioscope guidance. Institutional review board approval was not needed for this type of case report. A 53-year-old male with history of orthotopic liver transplantation 22 years earlier for primary sclerosing cholangitis was referred to interventional radiology for management of a severe biliary stricture after endoscopic retrograde approach failure due to altered anatomy. Following 21-gauge needle access into a peripheral right duct using ultrasound guidance, percutaneous transhe...
Objectives. In response to ACGME work-hour restrictions, residency programs that require continuo... more Objectives. In response to ACGME work-hour restrictions, residency programs that require continuous inpatient clinical care for educational objectives will be forced to increase the proportion of junior resident experience involved in shift work. Maintaining the balance of education over service at these levels will be a challenge, where a considerable amount of time must be spent gathering data for morning rounds and signing out patients at shift change. Patient safety is an issue with this new paradigm. We hypothesized that computerized sign-out would improve resident efficiency. Materials and Methods. A multidisciplinary clinical team collaborated to design a computerized rounding and sign-out (CSO) program to automate collection of clinical information in addition to a brief narrative describingongoingcare issues.Residentsreturned aselfadministered questionnaire before (n [ 168) and after implementation (n [ 83) examining: pre-rounding time, missed patients, handoff quality, and...
INTRODUCTION During the anhepatic phase of liver transplantation (LT), fibrinolytic activity incr... more INTRODUCTION During the anhepatic phase of liver transplantation (LT), fibrinolytic activity increases, since the liver clears tissue plasminogen activator (tPA). We hypothesize that patients who fail to reduce fibrinolytic activity following graft reperfusion will have an increased rate of early allograft dysfunction (EAD). METHODS Assessment of fibrinolysis in liver transplant recipients was quantified with thrombelastography (TEG) LY30. Changes in LY30 were assessed after graft reperfusion. The 30-min post-reperfusion LY30 was subtracted from the anhepatic LY30 quantifying fibrinolytic changes (delta-LY30). RESULTS Seventy-three primary LT patients were included in the analysis. Receiver operating characteristic curve (ROC) analysis identified an inflection point of delta-LY30-5.3% as a risk factor for EAD. EAD occurred in 44% of these patients compared to 5% in high delta-LY30 (p = 0.002). CONCLUSION LT recipients that develop hyperfibrinolysis who fail to reduce fibrinolytic activity 30 min after graft reperfusion had an EAD rate 8-fold higher than patients who had a large reduction in LY30 following reperfusion.
e15176 Background: MRI is new tool in the diagnosis and surveillance of localized prostate cancer... more e15176 Background: MRI is new tool in the diagnosis and surveillance of localized prostate cancer. Studies have examined the sensitivity and specificity of MRI for detecting and localizing prostate cancer as compared to radical prostatectomy specimens. We have previously demonstrated excellent correlation between 3-dimensional transperineal mapping biopsies (3DMB) to radical prostatectomy specimens. We hypothesize that MRI does not perform as well detecting smaller lesions of the prostate as larger ones. In this study, we retrospectively compare the correlation of 3DMB to 3T MRI-positive lesions. Methods: Between 2010 and 2011, 34 men with histologically-proven prostate cancer underwent 3T endorectal coil MRI and subsequent template-guided (5-mm grid) 3DMB. Three-dimensional computer models of the mapping biopsies were created using Proview software to identify clusters of positive biopsies likely belonging to a single lesion. MRIs were read by two trained radiologists blinded to 3D...
American journal of physiology. Renal physiology, 2014
The role of inflammation in oxalate-induced nephrolithiasis is debated. Our gene expression study... more The role of inflammation in oxalate-induced nephrolithiasis is debated. Our gene expression study indicated an increase in interleukin-2 receptor β (IL-2Rβ) mRNA in response to oxalate (Koul S, Khandrika L, Meacham RB, Koul HK. PLoS ONE 7: e43886, 2012). Herein, we evaluated IL-2Rβ expression and its downstream signaling pathway in HK-2 cells in an effort to understand the mechanisms of oxalate nephrotoxicity. HK-2 cells were exposed to oxalate for various time points in the presence or absence of SB203580, a specific p38 MAPK inhibitor. Gene expression data were analyzed by Ingenuity Pathway Analysis software. mRNA expression was quantitated via real-time PCR, and changes in protein expression/kinase activation were analyzed by Western blotting. Exposure of HK-2 cells to oxalate resulted in increased transcription of IL-2Rβ mRNA and increased protein levels. Oxalate treatment also activated the IL-2Rβ signaling pathway (JAK1/STAT5 phosphorylation). Moreover, the increase in IL-2Rβ protein was dependent upon p38 MAPK activity. These results suggest that oxalate-induced activation of the IL-2Rβ pathway may lead to a plethora of cellular changes, the most common of which is the induction of inflammation. These results suggest a central role for the p38 MAPK pathway in mediating the effects of oxalate in renal cells, and additional studies may provide the key to unlocking novel biochemical targets in stone disease.
In response to ACGME work-hour restrictions, residency programs that require continuous inpatient... more In response to ACGME work-hour restrictions, residency programs that require continuous inpatient clinical care for educational objectives will be forced to increase the proportion of junior resident experience involved in shift work. Maintaining the balance of education over service at these levels will be a challenge, where a considerable amount of time must be spent gathering data for morning rounds and signing out patients at shift change. Patient safety is an issue with this new paradigm. We hypothesized that computerized sign-out would improve resident efficiency.A multidisciplinary clinical team collaborated to design a computerized rounding and sign-out (CSO) program to automate collection of clinical information in addition to a brief narrative describing ongoing care issues. Residents returned a self-administered questionnaire before (n = 168) and after implementation (n = 83) examining: pre-rounding time, missed patients, handoff quality, and duty hours.Residents reported spending 11 fewer min/d pre-rounding (P = 0.006). After implementation, residents missed fewer patients on rounds (P = 0.01). A majority (70%) of responders stated that the new program helped them with duty hours.The current study demonstrates the reproducibility of the University of Washington model system for rounding and sign-out at an independent site, using basic infrastructure and leadership common to all residency programs. Developing a CSO was associated with a modest reduction in pre-rounding time and fewer patients missed on rounds. Although automating resident tasks may improve workflow in an increasingly complex hospital environment, structured handoff education and other institutional changes are necessary.
To evaluate and compare the outcomes of transvaginal repair of benign, primary, and recurrent ves... more To evaluate and compare the outcomes of transvaginal repair of benign, primary, and recurrent vesicovaginal fistulas (VVFs) treated without tissue interposition because, historically, tissue interposition with a vascularized flap has been advocated in both transabdominal and transvaginal repairs of recurrent VVFs. A retrospective chart review was conducted of 73 consecutive women with VVF and treated by a single surgeon (B.J.F.) between January 2003 and May 2012. Patients with a malignant etiology and/or prior irradiation were excluded as they required a more complex repair. All included VVFs were treated by a transvaginal approach with partial vaginal cuff excision without a tissue interposition. Patients were followed in our clinic postoperatively for 1 year and by telephone survey thereafter. Forty-nine patients met inclusion criteria: 25 primary and 24 recurrent. There was no statistical difference in patient age, fistula size, time to repair, or fistula etiology between the 2 groups. There has been no fistula recurrence in either group. Forty-one of 49 patients (84%) were discharged the same day as their surgery. Benign, recurrent VVFs are not synonymous with other complex fistulas that typically require tissue interposition. Our study demonstrates that transvaginal repair of benign, recurrent VVFs without tissue interposition can be equally successful as primary repairs without tissue interposition. The number of prior repairs should not be an independent factor in the use of tissue interposition, as previously suggested. We advocate an individualized approach to each VVF, only using tissue interposition when appropriate.
Biliary strictures occur frequently after liver transplantation [1, 2]. Percutaneous management w... more Biliary strictures occur frequently after liver transplantation [1, 2]. Percutaneous management with benign stricture protocol is associated with high rates of long-term clinical patency [2–4]. Rarely, strictures can be severe and crossing with a catheter can be difficult or impossible. In such cases, sharp recanalization is a more aggressive alternative. We report a case of severe biliary stricturing where these traditional catheter crossing techniques failed yet the stricture was crossed easily with cholangioscope guidance. Institutional review board approval was not needed for this type of case report. A 53-year-old male with history of orthotopic liver transplantation 22 years earlier for primary sclerosing cholangitis was referred to interventional radiology for management of a severe biliary stricture after endoscopic retrograde approach failure due to altered anatomy. Following 21-gauge needle access into a peripheral right duct using ultrasound guidance, percutaneous transhe...
Objectives. In response to ACGME work-hour restrictions, residency programs that require continuo... more Objectives. In response to ACGME work-hour restrictions, residency programs that require continuous inpatient clinical care for educational objectives will be forced to increase the proportion of junior resident experience involved in shift work. Maintaining the balance of education over service at these levels will be a challenge, where a considerable amount of time must be spent gathering data for morning rounds and signing out patients at shift change. Patient safety is an issue with this new paradigm. We hypothesized that computerized sign-out would improve resident efficiency. Materials and Methods. A multidisciplinary clinical team collaborated to design a computerized rounding and sign-out (CSO) program to automate collection of clinical information in addition to a brief narrative describingongoingcare issues.Residentsreturned aselfadministered questionnaire before (n [ 168) and after implementation (n [ 83) examining: pre-rounding time, missed patients, handoff quality, and...
INTRODUCTION During the anhepatic phase of liver transplantation (LT), fibrinolytic activity incr... more INTRODUCTION During the anhepatic phase of liver transplantation (LT), fibrinolytic activity increases, since the liver clears tissue plasminogen activator (tPA). We hypothesize that patients who fail to reduce fibrinolytic activity following graft reperfusion will have an increased rate of early allograft dysfunction (EAD). METHODS Assessment of fibrinolysis in liver transplant recipients was quantified with thrombelastography (TEG) LY30. Changes in LY30 were assessed after graft reperfusion. The 30-min post-reperfusion LY30 was subtracted from the anhepatic LY30 quantifying fibrinolytic changes (delta-LY30). RESULTS Seventy-three primary LT patients were included in the analysis. Receiver operating characteristic curve (ROC) analysis identified an inflection point of delta-LY30-5.3% as a risk factor for EAD. EAD occurred in 44% of these patients compared to 5% in high delta-LY30 (p = 0.002). CONCLUSION LT recipients that develop hyperfibrinolysis who fail to reduce fibrinolytic activity 30 min after graft reperfusion had an EAD rate 8-fold higher than patients who had a large reduction in LY30 following reperfusion.
e15176 Background: MRI is new tool in the diagnosis and surveillance of localized prostate cancer... more e15176 Background: MRI is new tool in the diagnosis and surveillance of localized prostate cancer. Studies have examined the sensitivity and specificity of MRI for detecting and localizing prostate cancer as compared to radical prostatectomy specimens. We have previously demonstrated excellent correlation between 3-dimensional transperineal mapping biopsies (3DMB) to radical prostatectomy specimens. We hypothesize that MRI does not perform as well detecting smaller lesions of the prostate as larger ones. In this study, we retrospectively compare the correlation of 3DMB to 3T MRI-positive lesions. Methods: Between 2010 and 2011, 34 men with histologically-proven prostate cancer underwent 3T endorectal coil MRI and subsequent template-guided (5-mm grid) 3DMB. Three-dimensional computer models of the mapping biopsies were created using Proview software to identify clusters of positive biopsies likely belonging to a single lesion. MRIs were read by two trained radiologists blinded to 3D...
American journal of physiology. Renal physiology, 2014
The role of inflammation in oxalate-induced nephrolithiasis is debated. Our gene expression study... more The role of inflammation in oxalate-induced nephrolithiasis is debated. Our gene expression study indicated an increase in interleukin-2 receptor β (IL-2Rβ) mRNA in response to oxalate (Koul S, Khandrika L, Meacham RB, Koul HK. PLoS ONE 7: e43886, 2012). Herein, we evaluated IL-2Rβ expression and its downstream signaling pathway in HK-2 cells in an effort to understand the mechanisms of oxalate nephrotoxicity. HK-2 cells were exposed to oxalate for various time points in the presence or absence of SB203580, a specific p38 MAPK inhibitor. Gene expression data were analyzed by Ingenuity Pathway Analysis software. mRNA expression was quantitated via real-time PCR, and changes in protein expression/kinase activation were analyzed by Western blotting. Exposure of HK-2 cells to oxalate resulted in increased transcription of IL-2Rβ mRNA and increased protein levels. Oxalate treatment also activated the IL-2Rβ signaling pathway (JAK1/STAT5 phosphorylation). Moreover, the increase in IL-2Rβ protein was dependent upon p38 MAPK activity. These results suggest that oxalate-induced activation of the IL-2Rβ pathway may lead to a plethora of cellular changes, the most common of which is the induction of inflammation. These results suggest a central role for the p38 MAPK pathway in mediating the effects of oxalate in renal cells, and additional studies may provide the key to unlocking novel biochemical targets in stone disease.
In response to ACGME work-hour restrictions, residency programs that require continuous inpatient... more In response to ACGME work-hour restrictions, residency programs that require continuous inpatient clinical care for educational objectives will be forced to increase the proportion of junior resident experience involved in shift work. Maintaining the balance of education over service at these levels will be a challenge, where a considerable amount of time must be spent gathering data for morning rounds and signing out patients at shift change. Patient safety is an issue with this new paradigm. We hypothesized that computerized sign-out would improve resident efficiency.A multidisciplinary clinical team collaborated to design a computerized rounding and sign-out (CSO) program to automate collection of clinical information in addition to a brief narrative describing ongoing care issues. Residents returned a self-administered questionnaire before (n = 168) and after implementation (n = 83) examining: pre-rounding time, missed patients, handoff quality, and duty hours.Residents reported spending 11 fewer min/d pre-rounding (P = 0.006). After implementation, residents missed fewer patients on rounds (P = 0.01). A majority (70%) of responders stated that the new program helped them with duty hours.The current study demonstrates the reproducibility of the University of Washington model system for rounding and sign-out at an independent site, using basic infrastructure and leadership common to all residency programs. Developing a CSO was associated with a modest reduction in pre-rounding time and fewer patients missed on rounds. Although automating resident tasks may improve workflow in an increasingly complex hospital environment, structured handoff education and other institutional changes are necessary.
To evaluate and compare the outcomes of transvaginal repair of benign, primary, and recurrent ves... more To evaluate and compare the outcomes of transvaginal repair of benign, primary, and recurrent vesicovaginal fistulas (VVFs) treated without tissue interposition because, historically, tissue interposition with a vascularized flap has been advocated in both transabdominal and transvaginal repairs of recurrent VVFs. A retrospective chart review was conducted of 73 consecutive women with VVF and treated by a single surgeon (B.J.F.) between January 2003 and May 2012. Patients with a malignant etiology and/or prior irradiation were excluded as they required a more complex repair. All included VVFs were treated by a transvaginal approach with partial vaginal cuff excision without a tissue interposition. Patients were followed in our clinic postoperatively for 1 year and by telephone survey thereafter. Forty-nine patients met inclusion criteria: 25 primary and 24 recurrent. There was no statistical difference in patient age, fistula size, time to repair, or fistula etiology between the 2 groups. There has been no fistula recurrence in either group. Forty-one of 49 patients (84%) were discharged the same day as their surgery. Benign, recurrent VVFs are not synonymous with other complex fistulas that typically require tissue interposition. Our study demonstrates that transvaginal repair of benign, recurrent VVFs without tissue interposition can be equally successful as primary repairs without tissue interposition. The number of prior repairs should not be an independent factor in the use of tissue interposition, as previously suggested. We advocate an individualized approach to each VVF, only using tissue interposition when appropriate.
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Papers by Thomas Pshak