Genotoxic effect of ethephon [(2-chloroethyl) phosphonic acid], a PGR that has replaced calcium c... more Genotoxic effect of ethephon [(2-chloroethyl) phosphonic acid], a PGR that has replaced calcium carbide as an agent for fruit ripening, was studied by using a root chromosome assay. Onion root meristems were cultured and analyzed after exposure with ethephon at concentrations of 200 ppm and 500 ppm for 2, 24, and 48 h. There was a time dependent decrease and an immediate toxic effect on dividing cells in mitotic index when compared to control (the mitotic indices of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 11.1%, 10.7%, and 8.9%, respectively; and with 500 ppm ethephon for 2, 24, and 48 h the mitotic indices were 10.8%, 10.2%, and 9.0% respectively). The total chromosomal aberrations increased with prolonged exposures indicating duration dependent effect compared to control (the chromosomal aberrations of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 14.5%, 15.6%, and 34.0% respectively; and with 500 ppm ethephon for 2, 24, and 48...
Breast cancer is a complex genetic disease that is caused in about 80% of the women due to variou... more Breast cancer is a complex genetic disease that is caused in about 80% of the women due to various environmental factors. The allopathic treatments which cause side effects are not appreciated in the recent years. Hence there has been a long-standing interest to find anticancer agents present in plants to eliminate side effects. Fimbristylis ovata (Burm.f.)Kern which is being used traditionally for the treatment of wounds and other associated diseases which could lead to malignancy. Since there has been no scientific data on the effect of Fimbristylis ovata on Vero cell line and MCF-7 cell line the concept of investigating its cytotoxic and anticancer potential was brought up. The tests were evaluated using MTT assay and it was noted that the crude methanol extract of Fimbristylis ovata could inhibit MCF-7 cell line at an IC 50 value of 73 and Vero cell line at an IC 50 value of 280.
Genotoxic effect of ethephon [(2-chloroethyl) phosphonic acid], a PGR that has replaced calcium c... more Genotoxic effect of ethephon [(2-chloroethyl) phosphonic acid], a PGR that has replaced calcium carbide as an agent for fruit ripening, was studied by using a root chromosome assay. Onion root meristems were cultured and analyzed after exposure with ethephon at concentrations of 200 ppm and 500 ppm for 2, 24, and 48 h. There was a time dependent decrease and an immediate toxic effect on dividing cells in mitotic index when compared to control (the mitotic indices of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 11.1%, 10.7%, and 8.9%, respectively; and with 500 ppm ethephon for 2, 24, and 48 h the mitotic indices were 10.8%, 10.2%, and 9.0% respectively). The total chromosomal aberrations increased with prolonged exposures indicating duration dependent effect compared to control (the chromosomal aberrations of root meristems treated with 200 ppm ethephon for 2, 24, and 48 h were 14.5%, 15.6%, and 34.0% respectively; and with 500 ppm ethephon for 2, 24, and 48...
Breast cancer is a complex genetic disease that is caused in about 80% of the women due to variou... more Breast cancer is a complex genetic disease that is caused in about 80% of the women due to various environmental factors. The allopathic treatments which cause side effects are not appreciated in the recent years. Hence there has been a long-standing interest to find anticancer agents present in plants to eliminate side effects. Fimbristylis ovata (Burm.f.)Kern which is being used traditionally for the treatment of wounds and other associated diseases which could lead to malignancy. Since there has been no scientific data on the effect of Fimbristylis ovata on Vero cell line and MCF-7 cell line the concept of investigating its cytotoxic and anticancer potential was brought up. The tests were evaluated using MTT assay and it was noted that the crude methanol extract of Fimbristylis ovata could inhibit MCF-7 cell line at an IC 50 value of 73 and Vero cell line at an IC 50 value of 280.
Uploads