L'invention concerne des reactifs ainsi que des methodes de criblage de liants specifiques de... more L'invention concerne des reactifs ainsi que des methodes de criblage de liants specifiques de structures de glycanes de cellules souches. En outre, l'invention concerne le criblage de reactifs de liaison supplementaires diriges contre des epitopes specifiques des glycanes sur les surfaces des cellules souches. Les liants preferes des structures de glycanes comprennent des proteines telles que les enzymes, les lectines et les anticorps.
Background Circulating human red blood cells (RBCs) consist of mature erythrocytes and immature r... more Background Circulating human red blood cells (RBCs) consist of mature erythrocytes and immature reticulocytes. Being anucleated, RBCs lack typical transcriptomes, but are known to contain small amounts of diverse long transcripts and microRNAs. However, the exact role and importance of these RNAs is lacking. Results To study this further, we explored the transcriptomes of RBCs and extracellular vesicles (EVs) of RBCs using next-generation sequencing. Furthermore, to understand the dynamics of the RBC transcriptome, we performed single-cell RNA sequencing on RBCs. An analysis of the single-cell transcriptomes revealed that approximately 10% of the cells contained detectable levels of mRNA and fell into three subpopulations based on their transcriptomes. Decrease in the mRNA quantity was observed across the populations. Qualitative changes included the differences in the globin transcripts and changes in the expression of ribosomal genes. A specific short splice form of a long non-cod...
L'invention concerne des reactifs ainsi que des methodes de criblage de liants specifiques de... more L'invention concerne des reactifs ainsi que des methodes de criblage de liants specifiques de structures de glycanes de cellules souches. En outre, l'invention concerne le criblage de reactifs de liaison supplementaires diriges contre des epitopes specifiques des glycanes sur les surfaces des cellules souches. Les liants preferes des structures de glycanes comprennent des proteines telles que les enzymes, les lectines et les anticorps.
Background Circulating human red blood cells (RBCs) consist of mature erythrocytes and immature r... more Background Circulating human red blood cells (RBCs) consist of mature erythrocytes and immature reticulocytes. Being anucleated, RBCs lack typical transcriptomes, but are known to contain small amounts of diverse long transcripts and microRNAs. However, the exact role and importance of these RNAs is lacking. Results To study this further, we explored the transcriptomes of RBCs and extracellular vesicles (EVs) of RBCs using next-generation sequencing. Furthermore, to understand the dynamics of the RBC transcriptome, we performed single-cell RNA sequencing on RBCs. An analysis of the single-cell transcriptomes revealed that approximately 10% of the cells contained detectable levels of mRNA and fell into three subpopulations based on their transcriptomes. Decrease in the mRNA quantity was observed across the populations. Qualitative changes included the differences in the globin transcripts and changes in the expression of ribosomal genes. A specific short splice form of a long non-cod...
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