The objective of the present study was to find the optimal conditions for micropropagation of Alt... more The objective of the present study was to find the optimal conditions for micropropagation of Alternanthera sp. The first experiment aimed to find the optimal sterilization of lateral bud meristems for in vitro culture. The results revealed that the best sterilization technique is to clean lateral bud meristem by bending shoot and dipping in 70% ethanol for 1 min. These shoots were then removed and immersed in 3% sodium hypochlorite solution for 10 min. This sterilization technique provided the highest survival percentage of 95% and the best microorganism removal. The second experiment aimed to determine the effects of the plant growth regulators benzyladenine (BA) on shoot proliferation. The shoot induction of sterile lateral bud meristems of Alternanthera sp. was then carried out in Murashige and Skoog (MS) media supplemented with benzyladenine (BA) at five different concentrations (0, 1, 2, 3 and 4 mg/l) under 25±2 °C and light condition for 4 weeks. It was found that MS media su...
The objective of the present study was to find the optimal conditions for micropropagation of Alt... more The objective of the present study was to find the optimal conditions for micropropagation of Alternanthera sp. The first experiment aimed to find the optimal sterilization of lateral bud meristems for in vitro culture. The results revealed that the best sterilization technique is to clean lateral bud meristem by bending shoot and dipping in 70% ethanol for 1 min. These shoots were then removed and immersed in 3% sodium hypochlorite solution for 10 min. This sterilization technique provided the highest survival percentage of 95% and the best microorganism removal. The second experiment aimed to determine the effects of the plant growth regulators benzyladenine (BA) on shoot proliferation. The shoot induction of sterile lateral bud meristems of Alternanthera sp. was then carried out in Murashige and Skoog (MS) media supplemented with benzyladenine (BA) at five different concentrations (0, 1, 2, 3 and 4 mg/l) under 25±2 °C and light condition for 4 weeks. It was found that MS media su...
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