Beneficial bacteria with antibacterial properties are attractive alternatives to chemical-based a... more Beneficial bacteria with antibacterial properties are attractive alternatives to chemical-based antibacterial or bactericidal agents. Our study sourced such bacteria from horticultural produce and environments to explore the mechanisms of their antimicrobial properties. Five strains of Pseudomonas fluorescens were studied that possessed antibacterial activity against the pathogen Listeria monocytogenes. The vegetative culture of these strains (Pseudomonas fluorescens-PFR46I06, Pseudomonas fluorescens-PFR46H06, Pseudomonas fluorescens-PFR46H07, Pseudomonas fluorescens-PFR46H08 and Pseudomonas fluorescens-PFR46H09) were tested against Listeria monocytogenes (n = 31), Listeria seeligeri (n = 1) and Listeria innocua (n = 1) isolated from seafood and horticultural sources and from clinical cases (n = 2) using solid media coculture and liquid media coculture. All Listeria strains were inhibited by all strains of P. fluorescens; however, P. fluorescens-PFR46H07, P. fluorescens-PFR46H08 and...
The data provides the guanine-cytosine (GC) contents for individual metabolic housekeeping genes ... more The data provides the guanine-cytosine (GC) contents for individual metabolic housekeeping genes and the individual genes investigated in the study from 19 C. jejuni genomes. It also provides the guanine-cytosine contents at the third codon position (GC3) for individual metabolic housekeeping genes and individual informational genes investigated in the study from 19 C. jejuni genomes.
Population genetics and phylogenetics have the potential to provide enormous insights into the ep... more Population genetics and phylogenetics have the potential to provide enormous insights into the epidemiology and ecology of disease causing pathogens. Molecular datasets are the basis to infer population structure, gene flow (between host populations and between different geographical locations) and to predict the evolutionary dynamics of pathogens. Campylobacter colonisation in food producing animals has been extensively studied and the population structure and host association of C. jejuni, the most commonly reported gastro-enteric pathogen, has also been well defined. In contrast, host-pathogen relationships and the population structure of C. jejuni in urban wild birds and pets have not been well defined on a wide range of spatial and/or temporal scales. A greater understanding of these details should allow disease control authorities to track the transmission of pathogens from one host species to another, identify the origin of pathogens and to better understand environmental fac...
Diagnosis of Mycobacterium bovis is important from the public health perspective since control me... more Diagnosis of Mycobacterium bovis is important from the public health perspective since control measures, treatment are very significant, particularly in wild animals. To diagnose M. bovis infection in wild animals polymerase chain reaction had ...
International Journal of Systematic and Evolutionary Microbiology
Six isolates of Campylobacter with similar non-standard colonial morphologies were identified dur... more Six isolates of Campylobacter with similar non-standard colonial morphologies were identified during studies isolating Campylobacter from bird faeces and rivers in New Zealand. Genomic (16S rRNA gene sequencing and whole genome analysis) and phenotypic (MALDI-TOF analysis and conventional biochemical tests) showed that the isolates form a monophyletic clade with genetic relationships to Campylobacter coli / Campylobacter jejuni and Campylobacter peloridis /Campylobacter amoricus. They may be distinguished from other Campylobacter by their MALDI-TOF spectral pattern, their florid α-haemolysis, their ability to grow anaerobically at 37 °C, and on 2 % NaCl nutrient agar, and their lack of hippuricase. This study shows that these isolates represent a novel species within the genus Campylobacter for which the name Campylobacter novaezeelandiae sp. nov. is proposed. The presence of C. novaezeelandiae in water may be a confounder for freshwater microbial risk assessment as they may not be ...
Beneficial bacteria with antibacterial properties are attractive alternatives to chemical-based a... more Beneficial bacteria with antibacterial properties are attractive alternatives to chemical-based antibacterial or bactericidal agents. Our study sourced such bacteria from horticultural produce and environments to explore the mechanisms of their antimicrobial properties. Five strains of Pseudomonas fluorescens were studied that possessed antibacterial activity against the pathogen Listeria monocytogenes. The vegetative culture of these strains (Pseudomonas fluorescens-PFR46I06, Pseudomonas fluorescens-PFR46H06, Pseudomonas fluorescens-PFR46H07, Pseudomonas fluorescens-PFR46H08 and Pseudomonas fluorescens-PFR46H09) were tested against Listeria monocytogenes (n = 31), Listeria seeligeri (n = 1) and Listeria innocua (n = 1) isolated from seafood and horticultural sources and from clinical cases (n = 2) using solid media coculture and liquid media coculture. All Listeria strains were inhibited by all strains of P. fluorescens; however, P. fluorescens-PFR46H07, P. fluorescens-PFR46H08 and...
The data provides the guanine-cytosine (GC) contents for individual metabolic housekeeping genes ... more The data provides the guanine-cytosine (GC) contents for individual metabolic housekeeping genes and the individual genes investigated in the study from 19 C. jejuni genomes. It also provides the guanine-cytosine contents at the third codon position (GC3) for individual metabolic housekeeping genes and individual informational genes investigated in the study from 19 C. jejuni genomes.
Population genetics and phylogenetics have the potential to provide enormous insights into the ep... more Population genetics and phylogenetics have the potential to provide enormous insights into the epidemiology and ecology of disease causing pathogens. Molecular datasets are the basis to infer population structure, gene flow (between host populations and between different geographical locations) and to predict the evolutionary dynamics of pathogens. Campylobacter colonisation in food producing animals has been extensively studied and the population structure and host association of C. jejuni, the most commonly reported gastro-enteric pathogen, has also been well defined. In contrast, host-pathogen relationships and the population structure of C. jejuni in urban wild birds and pets have not been well defined on a wide range of spatial and/or temporal scales. A greater understanding of these details should allow disease control authorities to track the transmission of pathogens from one host species to another, identify the origin of pathogens and to better understand environmental fac...
Diagnosis of Mycobacterium bovis is important from the public health perspective since control me... more Diagnosis of Mycobacterium bovis is important from the public health perspective since control measures, treatment are very significant, particularly in wild animals. To diagnose M. bovis infection in wild animals polymerase chain reaction had ...
International Journal of Systematic and Evolutionary Microbiology
Six isolates of Campylobacter with similar non-standard colonial morphologies were identified dur... more Six isolates of Campylobacter with similar non-standard colonial morphologies were identified during studies isolating Campylobacter from bird faeces and rivers in New Zealand. Genomic (16S rRNA gene sequencing and whole genome analysis) and phenotypic (MALDI-TOF analysis and conventional biochemical tests) showed that the isolates form a monophyletic clade with genetic relationships to Campylobacter coli / Campylobacter jejuni and Campylobacter peloridis /Campylobacter amoricus. They may be distinguished from other Campylobacter by their MALDI-TOF spectral pattern, their florid α-haemolysis, their ability to grow anaerobically at 37 °C, and on 2 % NaCl nutrient agar, and their lack of hippuricase. This study shows that these isolates represent a novel species within the genus Campylobacter for which the name Campylobacter novaezeelandiae sp. nov. is proposed. The presence of C. novaezeelandiae in water may be a confounder for freshwater microbial risk assessment as they may not be ...
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