Shoot tips obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated... more Shoot tips obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated in calcium alginate beads for short-term storage and germplasm exchange. A gelling matrix of 3% sodium alginate and 100mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Maximum percent response for conversion of encapsulated shoot tips into plantlets was obtained on growth regulator free full strength liquid MS medium. The regrowth ability of encapsulated shoot tips was affected by medium strength and sucrose concentrations in the medium. Encapsulated shoot tips could be stored at low temperature (4°C) up to 30 days with a survival frequency of 25%. After 60 days of storage under minimal growth conditions (sucrose lacking medium), about 75% encapsulated shoot tips were converted into plantlets when subcultured on 3% sucrose containing medium. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully.
Present study demonstrates the effect of sucrose and ABA on germination of encapsulated somatic e... more Present study demonstrates the effect of sucrose and ABA on germination of encapsulated somatic embryos of guava (Psidium guajava L.). Sucrose and ABA at different concentrations were also evaluated for their effects on maturation and germination of somatic embryos. Mature somatic embryos developed on MS medium containing high concentration of sucrose (10%) or ABA (1.0mgl−1) showed inhibition in germination if they continued to be in same medium for 4 weeks. With increasing concentrations of sucrose (3–9%) or ABA (0.01–1.0mgl−1) in medium, percent germination of encapsulated somatic embryos decreased significantly. Encapsulated somatic embryos after storage on MS medium supplemented with 9% sucrose or 1mgl−1 ABA for different duration (0–60 days) germinated when they were transferred to medium containing 3% sucrose. About 20.8% and 37.5% encapsulated somatic embryos germinated after storage on ABA (1mgl−1) or sucrose (9%) for 60 days, respectively. Temporarily suppression in germination of encapsulated somatic embryos by high concentration of sucrose or ABA may be important for short-term conservation of elite genotype of guava.
The main objective of this paper is to study the electric field distributions around a standard p... more The main objective of this paper is to study the electric field distributions around a standard post insulator for the power frequency, lightning impulse (1.2/50 µs) and switching impulse (250/2500 µs) voltages under icing conditions, and these were computed numerically using the finite element method. Thin glaze coating on the insulator requires a very large number of elements for finite
Page 1. Finite element modelling of ionized field quantities around a monopolar HVDCtransmission ... more Page 1. Finite element modelling of ionized field quantities around a monopolar HVDCtransmission line ... Indust. Appl. 20 160712 [5] Qin B, Sheng J, Yan Z and Gela G 1988 Accurate calculation of ion flow field under HVDC bipolar transmission lines IEEE Trans. ...
In Vitro Cellular & Developmental Biology - Plant, 2006
... multiplication. Low salt concentration has also been reported to be superior for shoot prolif... more ... multiplication. Low salt concentration has also been reported to be superior for shoot proliferation in Pinus ponderosa (Tuskan et al., 1990), Dendrocalamus strictus (Singh et al., 2001) and T. arjuna (Pandey and Jaiswal, 2002). Pandey ...
Shoot tips obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated... more Shoot tips obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated in calcium alginate beads for short-term storage and germplasm exchange. A gelling matrix of 3% sodium alginate and 100mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Maximum percent response for conversion of encapsulated shoot tips into plantlets was obtained on growth regulator free full strength liquid MS medium. The regrowth ability of encapsulated shoot tips was affected by medium strength and sucrose concentrations in the medium. Encapsulated shoot tips could be stored at low temperature (4°C) up to 30 days with a survival frequency of 25%. After 60 days of storage under minimal growth conditions (sucrose lacking medium), about 75% encapsulated shoot tips were converted into plantlets when subcultured on 3% sucrose containing medium. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully.
Present study demonstrates the effect of sucrose and ABA on germination of encapsulated somatic e... more Present study demonstrates the effect of sucrose and ABA on germination of encapsulated somatic embryos of guava (Psidium guajava L.). Sucrose and ABA at different concentrations were also evaluated for their effects on maturation and germination of somatic embryos. Mature somatic embryos developed on MS medium containing high concentration of sucrose (10%) or ABA (1.0mgl−1) showed inhibition in germination if they continued to be in same medium for 4 weeks. With increasing concentrations of sucrose (3–9%) or ABA (0.01–1.0mgl−1) in medium, percent germination of encapsulated somatic embryos decreased significantly. Encapsulated somatic embryos after storage on MS medium supplemented with 9% sucrose or 1mgl−1 ABA for different duration (0–60 days) germinated when they were transferred to medium containing 3% sucrose. About 20.8% and 37.5% encapsulated somatic embryos germinated after storage on ABA (1mgl−1) or sucrose (9%) for 60 days, respectively. Temporarily suppression in germination of encapsulated somatic embryos by high concentration of sucrose or ABA may be important for short-term conservation of elite genotype of guava.
The main objective of this paper is to study the electric field distributions around a standard p... more The main objective of this paper is to study the electric field distributions around a standard post insulator for the power frequency, lightning impulse (1.2/50 µs) and switching impulse (250/2500 µs) voltages under icing conditions, and these were computed numerically using the finite element method. Thin glaze coating on the insulator requires a very large number of elements for finite
Page 1. Finite element modelling of ionized field quantities around a monopolar HVDCtransmission ... more Page 1. Finite element modelling of ionized field quantities around a monopolar HVDCtransmission line ... Indust. Appl. 20 160712 [5] Qin B, Sheng J, Yan Z and Gela G 1988 Accurate calculation of ion flow field under HVDC bipolar transmission lines IEEE Trans. ...
In Vitro Cellular & Developmental Biology - Plant, 2006
... multiplication. Low salt concentration has also been reported to be superior for shoot prolif... more ... multiplication. Low salt concentration has also been reported to be superior for shoot proliferation in Pinus ponderosa (Tuskan et al., 1990), Dendrocalamus strictus (Singh et al., 2001) and T. arjuna (Pandey and Jaiswal, 2002). Pandey ...
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