INTRODUCTION Heart valve replacements are required for a number of heart diseases and replacement... more INTRODUCTION Heart valve replacements are required for a number of heart diseases and replacements, as well as for both pediatric and adult populations. It is estimated that 2.5% of the population has valvular heart disease [1], and annually, over 250,000 people worldwide receive heart valve replacements [1]. Tissue engineering (TE) strategies hold the most promise for permanently restoring the damaged heart valve or nearby tissue. The premise of tissue engineering is to replace or facilitate the regrowth of damaged tissues by creating biodegradable, biocompatible, and mechanically analogous tissue substitutes – such bioresorbable scaffolds. The general method of growing TE analogs involves seeding cells on a fibrous, porous, biomimetic scaffold. As the cells infiltrate the scaffold structure, they grow, divide, and eventually degrade the scaffold matrix and replace it with natural extracellular matrix (ECM). During this process, the scaffold mimics the cells’ ECM via its fibrous st...
La presente invention concerne une composition bicouche destinee a l'administration d'ace... more La presente invention concerne une composition bicouche destinee a l'administration d'acetaminophene et de tramadol durant une periode d'au moins douze heures suivant l'administration initiale. Une administration unique de la composition bicouche peut provoquer une analgesie debutant entre moins d'une demi-heure et environ une heure apres l'administration initiale, avec une duree d'au moins douze heures apres ladite administration initiale.
A solid composition for oral administration of at least one pharmaceutically active agent, compri... more A solid composition for oral administration of at least one pharmaceutically active agent, comprising: (a) a first layer comprising a first population of controlled release microparticles having a pharmaceutically active agent disposed therein; (B) a second layer comprising a pharmaceutically active agent disposed therein; and (c) a superabsorbent material disposed within the first layer, the second layer, or both the first layer and the second layer, wherein the composition, (i) when intact and exposed to a aqueous environment, initially, the pharmaceutically active agent disposed in the second layer is released to the pharmaceutically active agent disposed in the first layer, and (ii) when triturated faster rate and exposed to an aqueous environment, the superabsorbent material it swells to create a hard gel that traps the microparticles, after which the hard gel, microparticles or both the hard gel as the microparticles enables controlled release of at least the pharmaceutically ...
Hydrogel-based biomaterials have advanced bone tissue engineering approaches in the last decade, ... more Hydrogel-based biomaterials have advanced bone tissue engineering approaches in the last decade, through their ability to serve as a carrier for potent growth factor, bone morphogenic protein-2 (BM...
Journal of Biomedical Materials Research Part A, 2020
Polysaccharides are explored for various tissue engineering applications due to their inherent cy... more Polysaccharides are explored for various tissue engineering applications due to their inherent cytocompatibility and ability to form bulk hydrogels. However, bulk hydrogels offer poor control over their microarchitecture and multi-scale hierarchy, parameters important to recreate extracellular matrix-mimetic microenvironment. Here, we developed a versatile platform technology to self-assemble oppositely charged polysaccharides into multi-scale fibrous hydrogels with controlled anisotropic microarchitecture. We employed polyionic complexation through microfluidic flow of positively charged polysaccharide, chitosan, along with one of the three negatively charged polysaccharides: alginate, gellan gum, and kappa carrageenan. These hydrogels were composed of micro-scale fibers, which in turn were made of sub-micron fibrils confirming multi-scale hierarchy. Fibrous hydrogels showed strong tensile mechanical properties, which were further modulated by encapsulation of shape-specific antioxidant cerium oxide nanoparticles. Specifically, hydrogels with chitosan and gellan gum showed more than eight times higher tensile strength compared to the other two pairs. Incorporation of sphere-shaped cerium oxide nanoparticles in chitosan and gellan gum further reinforced the fibrous hydrogels and increased tensile strength by 40%. Altogether, our automated hydrogel fabrication platform allows fabrication of bioinspired biomaterials with scope for one-step encapsulation of small molecules and nanoparticles without chemical modification or use of chemical crosslinkers. This article is protected by copyright. All rights reserved.
Human immunodeficiency virus (HIV) infection and unintended pregnancy, which can lead to life-thr... more Human immunodeficiency virus (HIV) infection and unintended pregnancy, which can lead to life-threatening complications, are two major burdens for female reproductive health. To address these pressing health issues, multipurpose prevention technologies (MPTs) are proposed to deliver two or more drugs simultaneously. MPTs could offer several benefits for users such as improved convenience, increased effectiveness, reduced cost, and decreased environmental burden. Here, we report the development, and in vitro and in vivo assessment of a bioadhesive vaginal film as a coitally-independent MPT dosage form for delivering dapivirine (DPV) and levonorgestrel (LNG) to prevent HIV infection and unintended pregnancy, respectively. After confirming the feasibility of bioadhesive film use for weekly drug delivery in vivo through colpophotography and MRI evaluation, the pharmacokinetics (PK) of DPV/LNG single entity and combination bioadhesive films was investigated in pigtailed macaques (n = 5)....
A process for producing microparticles that stabilize and / or control the release of an agent di... more A process for producing microparticles that stabilize and / or control the release of an agent disposed therein, the method (A) comprising providing a solution comprising a mixture of 5% w / w to 20% w / w crosslinked starch of high amylose content and the agent to be released; and (B) spray drying the mixture of step (a) in a spray dryer to produce microparticles having an average diameter of 1 μm to 200 μm.
A urethane-based analogue containing an azo aromatic linkage in the backbone was synthesized for ... more A urethane-based analogue containing an azo aromatic linkage in the backbone was synthesized for use in colon-specific delivery systems by reacting toluene-2,6-diisocyanate with a mixture of an aromatic azo diol, (bis-4-hydroxyphenyl)-4,4′-diazobiphenyl, poly(ethylene glycol) (Mn = 4000; number-average molecular weight) and 1,2-propanediol (propylene glycol). The resultant compounds (UR-1 and UR-2) were characterized by IR spectroscopy, 1H NMR spectroscopy, DSC studies, X-ray diffraction studies and molecular weight determination by gel permeation chromatography. The compounds exhibited low molecular weight, lacked film-forming properties and crystallinity in the structure. An in-vitro bacterial degradation test to demonstrate the susceptibility of azo bond to bacterial enzymes was performed using media inoculated with lactobacillus culture. The results indicated degradation of films by azoreductase. In-vitro permeation of 5-aminosalicylic acid was studied in control and lactobacill...
INTRODUCTION Heart valve replacements are required for a number of heart diseases and replacement... more INTRODUCTION Heart valve replacements are required for a number of heart diseases and replacements, as well as for both pediatric and adult populations. It is estimated that 2.5% of the population has valvular heart disease [1], and annually, over 250,000 people worldwide receive heart valve replacements [1]. Tissue engineering (TE) strategies hold the most promise for permanently restoring the damaged heart valve or nearby tissue. The premise of tissue engineering is to replace or facilitate the regrowth of damaged tissues by creating biodegradable, biocompatible, and mechanically analogous tissue substitutes – such bioresorbable scaffolds. The general method of growing TE analogs involves seeding cells on a fibrous, porous, biomimetic scaffold. As the cells infiltrate the scaffold structure, they grow, divide, and eventually degrade the scaffold matrix and replace it with natural extracellular matrix (ECM). During this process, the scaffold mimics the cells’ ECM via its fibrous st...
La presente invention concerne une composition bicouche destinee a l'administration d'ace... more La presente invention concerne une composition bicouche destinee a l'administration d'acetaminophene et de tramadol durant une periode d'au moins douze heures suivant l'administration initiale. Une administration unique de la composition bicouche peut provoquer une analgesie debutant entre moins d'une demi-heure et environ une heure apres l'administration initiale, avec une duree d'au moins douze heures apres ladite administration initiale.
A solid composition for oral administration of at least one pharmaceutically active agent, compri... more A solid composition for oral administration of at least one pharmaceutically active agent, comprising: (a) a first layer comprising a first population of controlled release microparticles having a pharmaceutically active agent disposed therein; (B) a second layer comprising a pharmaceutically active agent disposed therein; and (c) a superabsorbent material disposed within the first layer, the second layer, or both the first layer and the second layer, wherein the composition, (i) when intact and exposed to a aqueous environment, initially, the pharmaceutically active agent disposed in the second layer is released to the pharmaceutically active agent disposed in the first layer, and (ii) when triturated faster rate and exposed to an aqueous environment, the superabsorbent material it swells to create a hard gel that traps the microparticles, after which the hard gel, microparticles or both the hard gel as the microparticles enables controlled release of at least the pharmaceutically ...
Hydrogel-based biomaterials have advanced bone tissue engineering approaches in the last decade, ... more Hydrogel-based biomaterials have advanced bone tissue engineering approaches in the last decade, through their ability to serve as a carrier for potent growth factor, bone morphogenic protein-2 (BM...
Journal of Biomedical Materials Research Part A, 2020
Polysaccharides are explored for various tissue engineering applications due to their inherent cy... more Polysaccharides are explored for various tissue engineering applications due to their inherent cytocompatibility and ability to form bulk hydrogels. However, bulk hydrogels offer poor control over their microarchitecture and multi-scale hierarchy, parameters important to recreate extracellular matrix-mimetic microenvironment. Here, we developed a versatile platform technology to self-assemble oppositely charged polysaccharides into multi-scale fibrous hydrogels with controlled anisotropic microarchitecture. We employed polyionic complexation through microfluidic flow of positively charged polysaccharide, chitosan, along with one of the three negatively charged polysaccharides: alginate, gellan gum, and kappa carrageenan. These hydrogels were composed of micro-scale fibers, which in turn were made of sub-micron fibrils confirming multi-scale hierarchy. Fibrous hydrogels showed strong tensile mechanical properties, which were further modulated by encapsulation of shape-specific antioxidant cerium oxide nanoparticles. Specifically, hydrogels with chitosan and gellan gum showed more than eight times higher tensile strength compared to the other two pairs. Incorporation of sphere-shaped cerium oxide nanoparticles in chitosan and gellan gum further reinforced the fibrous hydrogels and increased tensile strength by 40%. Altogether, our automated hydrogel fabrication platform allows fabrication of bioinspired biomaterials with scope for one-step encapsulation of small molecules and nanoparticles without chemical modification or use of chemical crosslinkers. This article is protected by copyright. All rights reserved.
Human immunodeficiency virus (HIV) infection and unintended pregnancy, which can lead to life-thr... more Human immunodeficiency virus (HIV) infection and unintended pregnancy, which can lead to life-threatening complications, are two major burdens for female reproductive health. To address these pressing health issues, multipurpose prevention technologies (MPTs) are proposed to deliver two or more drugs simultaneously. MPTs could offer several benefits for users such as improved convenience, increased effectiveness, reduced cost, and decreased environmental burden. Here, we report the development, and in vitro and in vivo assessment of a bioadhesive vaginal film as a coitally-independent MPT dosage form for delivering dapivirine (DPV) and levonorgestrel (LNG) to prevent HIV infection and unintended pregnancy, respectively. After confirming the feasibility of bioadhesive film use for weekly drug delivery in vivo through colpophotography and MRI evaluation, the pharmacokinetics (PK) of DPV/LNG single entity and combination bioadhesive films was investigated in pigtailed macaques (n = 5)....
A process for producing microparticles that stabilize and / or control the release of an agent di... more A process for producing microparticles that stabilize and / or control the release of an agent disposed therein, the method (A) comprising providing a solution comprising a mixture of 5% w / w to 20% w / w crosslinked starch of high amylose content and the agent to be released; and (B) spray drying the mixture of step (a) in a spray dryer to produce microparticles having an average diameter of 1 μm to 200 μm.
A urethane-based analogue containing an azo aromatic linkage in the backbone was synthesized for ... more A urethane-based analogue containing an azo aromatic linkage in the backbone was synthesized for use in colon-specific delivery systems by reacting toluene-2,6-diisocyanate with a mixture of an aromatic azo diol, (bis-4-hydroxyphenyl)-4,4′-diazobiphenyl, poly(ethylene glycol) (Mn = 4000; number-average molecular weight) and 1,2-propanediol (propylene glycol). The resultant compounds (UR-1 and UR-2) were characterized by IR spectroscopy, 1H NMR spectroscopy, DSC studies, X-ray diffraction studies and molecular weight determination by gel permeation chromatography. The compounds exhibited low molecular weight, lacked film-forming properties and crystallinity in the structure. An in-vitro bacterial degradation test to demonstrate the susceptibility of azo bond to bacterial enzymes was performed using media inoculated with lactobacillus culture. The results indicated degradation of films by azoreductase. In-vitro permeation of 5-aminosalicylic acid was studied in control and lactobacill...
Uploads
Papers by Vinayak Sant