Expression of S-Adenosylmethionine synthetase (SAMS) gene in pigeon pea (Cajanus cajan L.) was an... more Expression of S-Adenosylmethionine synthetase (SAMS) gene in pigeon pea (Cajanus cajan L.) was analyzed by qRT PCR during abiotic stresses viz., drought, heavy metal (CdCl2) and cold. Maximum expression of SAMS gene in the leaves were observed at 3 days after drought stress with 15% PEG. Conversely, its expression was not detected in leaves and roots at cadmium stress but transcripts were down regulated as compared to the control. After 6 days of stress expression of SAMS gene was increased in leaves and roots as compared to the control but it was lower than its expression at 3 days after stress. The activities of antioxidative enzymes like glutathione reductase, glutathione-s-transferase, ascorbate peroxidase and metabolite constituents like polyamines and glycine betaine were also analyzed. The activities of antioxidative enzymes and concentration of glycine betaine showed remarkable increase in response to all stresses, except ascorbate peroxidase in heavy metal stress.
ABSTRACT Phyto-oxylipins are a group of biologically active molecules that play an important role... more ABSTRACT Phyto-oxylipins are a group of biologically active molecules that play an important role in plant defense. Their production begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases (LOXs; EC 1.13.11.12) to form 9- or 13-hydroperoxides. These are substrates for several enzymes involved in the synthesis of final oxylipins, which can act as signal molecules and/or direct antimicrobials. Recent completion of the castor bean genome sequence now permits genome-wide analysis of the LOX gene family in castor as well as comparison with LOX in Arabidopsis. We identified 12 candidate LOX genes in the castor bean genome. Phylogenetic analysis indicated that these LOX members cluster into two groups, designated types 1 and 2, as expected from previous studies. Out of which six LOX gene specific primers were designed to amplify castor LOX genes i.e. LOX1, LOX2, LOX3, LOX4, LOX5 and Dox. Sequence analysis showed conserved five iron binding sites in the LOX domain of all the Rc-LOX, however, only LOX5 contained consensus (positions 547, 556, and 715) histidines residue. Expression analysis of LOX2, 3, 4, 5 and DOX genes in resistant and susceptible genotypes of castor at 0 days after infection (DAI), 5 DAI and 10 DAI (30 days after sowing) was carried out using quantitative real time (RT)-PCR during castor bean–Fusarium oxysporium f. sp. ricini interaction. Results suggest that 2 (LOX2 and LOX5) of 6 Rc-LOX genes were detectable. Resistant genotypes (48-1 and SKP-84) exhibited appreciably higher expression of LOX5 during castor bean–F. oxysporium interaction, which further suggest the participation of Rc-LOX5, a type-1 LOX predicted to be 9-LOX, in wilt resistance.
Expression of S-Adenosylmethionine synthetase (SAMS) gene in pigeon pea (Cajanus cajan L.) was an... more Expression of S-Adenosylmethionine synthetase (SAMS) gene in pigeon pea (Cajanus cajan L.) was analyzed by qRT PCR during abiotic stresses viz., drought, heavy metal (CdCl2) and cold. Maximum expression of SAMS gene in the leaves were observed at 3 days after drought stress with 15% PEG. Conversely, its expression was not detected in leaves and roots at cadmium stress but transcripts were down regulated as compared to the control. After 6 days of stress expression of SAMS gene was increased in leaves and roots as compared to the control but it was lower than its expression at 3 days after stress. The activities of antioxidative enzymes like glutathione reductase, glutathione-s-transferase, ascorbate peroxidase and metabolite constituents like polyamines and glycine betaine were also analyzed. The activities of antioxidative enzymes and concentration of glycine betaine showed remarkable increase in response to all stresses, except ascorbate peroxidase in heavy metal stress.
ABSTRACT Phyto-oxylipins are a group of biologically active molecules that play an important role... more ABSTRACT Phyto-oxylipins are a group of biologically active molecules that play an important role in plant defense. Their production begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases (LOXs; EC 1.13.11.12) to form 9- or 13-hydroperoxides. These are substrates for several enzymes involved in the synthesis of final oxylipins, which can act as signal molecules and/or direct antimicrobials. Recent completion of the castor bean genome sequence now permits genome-wide analysis of the LOX gene family in castor as well as comparison with LOX in Arabidopsis. We identified 12 candidate LOX genes in the castor bean genome. Phylogenetic analysis indicated that these LOX members cluster into two groups, designated types 1 and 2, as expected from previous studies. Out of which six LOX gene specific primers were designed to amplify castor LOX genes i.e. LOX1, LOX2, LOX3, LOX4, LOX5 and Dox. Sequence analysis showed conserved five iron binding sites in the LOX domain of all the Rc-LOX, however, only LOX5 contained consensus (positions 547, 556, and 715) histidines residue. Expression analysis of LOX2, 3, 4, 5 and DOX genes in resistant and susceptible genotypes of castor at 0 days after infection (DAI), 5 DAI and 10 DAI (30 days after sowing) was carried out using quantitative real time (RT)-PCR during castor bean–Fusarium oxysporium f. sp. ricini interaction. Results suggest that 2 (LOX2 and LOX5) of 6 Rc-LOX genes were detectable. Resistant genotypes (48-1 and SKP-84) exhibited appreciably higher expression of LOX5 during castor bean–F. oxysporium interaction, which further suggest the participation of Rc-LOX5, a type-1 LOX predicted to be 9-LOX, in wilt resistance.
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Papers by Vipul Parekh