The high mobility group non-histone chromosomal proteins (HMG) from chicken thrombocytes were com... more The high mobility group non-histone chromosomal proteins (HMG) from chicken thrombocytes were compared with those from chicken erythrocytes. In terms of their extractability, electrophoretic behaviour in polyacrylamide gels containing 2.5 M-urea, and amino acid composition, the HMG proteins of these cells were indistinguishable. However, microcomplement fixation assay performed in the presence of antiserum against HMG-1 and HMG-2 revealed that these high molecular weight HMG proteins of chicken thrombocytes and erythrocytes are not cross-reactive. Electrophoretic analysis of HMG-1 and HMG-2 from these two types of cells in polyacrylamide gels containing 6.25 M-urea suggested differences in the HMG-2 patterns.
... 856 LEOKADIA KLYSZEJKO-STEFANOWICZ and WANDA M. KRAJEWSKA by the changes in all chromatin pro... more ... 856 LEOKADIA KLYSZEJKO-STEFANOWICZ and WANDA M. KRAJEWSKA by the changes in all chromatin protein fractions (Figs 3-5). A significant decrease of the relative con-tent of the proteins with the molecular weights 20,000, 62,000 and 79,000 and the ... Ada Unir. ...
Polish journal of pathology : official journal of the Polish Society of Pathologists, 2006
This study was carried out to evaluate the loss of heterozygosity (LOH) and microsatellite instab... more This study was carried out to evaluate the loss of heterozygosity (LOH) and microsatellite instability (MSI) in breast cancer, in the 12p13.3 and 1p32 chromosomal regions where RAD52 and RAD54 genes are localized. Polymorphic markers D12S98, D12S1698 for RAD52 and D1S209, D1S411 for RAD54 were used. Relationships between LOH and clinicopathological parameters, i.e. tumor type and grade, patient's age, steroid receptors status and lymph node and distal metastases were assessed. For alleles frequency estimation 100 primary breast cancers were tested. DNA isolated from paraffin-embedded tissues and their matched blood samples were analyzed for PCR-based LOH and MSI by fluorescence-based DNA sequencing technology. In analyzed cases LOH was found in 14% and 11% of informative cases for D12S98 and D12S1698 markers, respectively and in 18% and 17% of informative cases for D1S209 and D1S411 markers, respectively. The highest frequency of MSI was identified at loci D12S98 (10%) and D1S209 (11%). Significant correlations between RAD52 and RAD54 regions with concomitant LOH and histological type and progesterone receptor status were observed. In the case of RAD54 further correlations with respect to tumor grade and the presence of distal metastases were noticed.
The high mobility group non-histone chromosomal proteins (HMG) from chicken thrombocytes were com... more The high mobility group non-histone chromosomal proteins (HMG) from chicken thrombocytes were compared with those from chicken erythrocytes. In terms of their extractability, electrophoretic behaviour in polyacrylamide gels containing 2.5 M-urea, and amino acid composition, the HMG proteins of these cells were indistinguishable. However, microcomplement fixation assay performed in the presence of antiserum against HMG-1 and HMG-2 revealed that these high molecular weight HMG proteins of chicken thrombocytes and erythrocytes are not cross-reactive. Electrophoretic analysis of HMG-1 and HMG-2 from these two types of cells in polyacrylamide gels containing 6.25 M-urea suggested differences in the HMG-2 patterns.
... 856 LEOKADIA KLYSZEJKO-STEFANOWICZ and WANDA M. KRAJEWSKA by the changes in all chromatin pro... more ... 856 LEOKADIA KLYSZEJKO-STEFANOWICZ and WANDA M. KRAJEWSKA by the changes in all chromatin protein fractions (Figs 3-5). A significant decrease of the relative con-tent of the proteins with the molecular weights 20,000, 62,000 and 79,000 and the ... Ada Unir. ...
Polish journal of pathology : official journal of the Polish Society of Pathologists, 2006
This study was carried out to evaluate the loss of heterozygosity (LOH) and microsatellite instab... more This study was carried out to evaluate the loss of heterozygosity (LOH) and microsatellite instability (MSI) in breast cancer, in the 12p13.3 and 1p32 chromosomal regions where RAD52 and RAD54 genes are localized. Polymorphic markers D12S98, D12S1698 for RAD52 and D1S209, D1S411 for RAD54 were used. Relationships between LOH and clinicopathological parameters, i.e. tumor type and grade, patient's age, steroid receptors status and lymph node and distal metastases were assessed. For alleles frequency estimation 100 primary breast cancers were tested. DNA isolated from paraffin-embedded tissues and their matched blood samples were analyzed for PCR-based LOH and MSI by fluorescence-based DNA sequencing technology. In analyzed cases LOH was found in 14% and 11% of informative cases for D12S98 and D12S1698 markers, respectively and in 18% and 17% of informative cases for D1S209 and D1S411 markers, respectively. The highest frequency of MSI was identified at loci D12S98 (10%) and D1S209 (11%). Significant correlations between RAD52 and RAD54 regions with concomitant LOH and histological type and progesterone receptor status were observed. In the case of RAD54 further correlations with respect to tumor grade and the presence of distal metastases were noticed.
Uploads
Papers by Wanda Krajewska