Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its... more Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its flexibility and low cost . Poly(methyl methacrylate) (PMMA) attracts growing interest in microfluidics research community due to its low cost, high transparency, good mechanical and chemical properties. Combining PMMA with PDMS allows a high degree of flexibility in designing microfluidic devices. Bonding of PMMA to PDMS is a critical step for this hybrid approach. We present here a novel technique for bonding PMMA to PDMS. This technique is simple, fast and reliable. A dry adhesive layer was laminated onto the PMMA substrate. A pre-cured PDMS mixture was spin coated onto the adhesive to form a PDMS membrane. The bonding quality and the strength of the PDMS/adhesive membrane was tested using a precision pressure source. The results show that the bond can hold a pressure up to 20 kPa.
The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can... more The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can act as cholinesterase inhibitors, that disrupts neuromuscular transmission. As these nerve agents are colorless, odorless and highly toxic, they can be introduced into drinking water as a means of terrorist sabotage. Hence, numerous innovative devices and methods have been developed for rapid detection of these organophosphates. Microfluidic technology allows the implementation of fast and sensitive detection of Sarin. In this paper, a micro-total analysis systems (TAS), also known as Lab-on-a-chip, fitted with an optical detection system has been developed to analyze the presence of the nerve agent sarin in water samples. In the present set-up, inhibition of co-introduced cholinesterase and water samples containing trace amounts of nerve agent sarin into the microfluidic device was used as the basis for selective detection of sarin. The device was fabricated using polymeric micromachining with PMMA (poly (methymethacrylate)) as the substrate material. A chromophore was utilized to measure the activity of remnant cholinesterase activity, which is inversely related to the amount of sarin present in the water samples. Comparisons were made between two different optical detection techniques and the findings will be presented in this paper. The presented measurement method is simple, fast and as sensitive as Gas Chromatography.
Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its... more Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its flexibility and low cost . Poly(methyl methacrylate) (PMMA) attracts growing interest in microfluidics research community due to its low cost, high transparency, good mechanical and chemical properties. Combining PMMA with PDMS allows a high degree of flexibility in designing microfluidic devices. Bonding of PMMA to PDMS is a critical step for this hybrid approach. We present here a novel technique for bonding PMMA to PDMS. This technique is simple, fast and reliable. A dry adhesive layer was laminated onto the PMMA substrate. A pre-cured PDMS mixture was spin coated onto the adhesive to form a PDMS membrane. The bonding quality and the strength of the PDMS/adhesive membrane was tested using a precision pressure source. The results show that the bond can hold a pressure up to 20 kPa.
The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can... more The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can act as cholinesterase inhibitors, that disrupts neuromuscular transmission. As these nerve agents are colorless, odorless and highly toxic, they can be introduced into drinking water as a means of terrorist sabotage. Hence, numerous innovative devices and methods have been developed for rapid detection of these organophosphates. Microfluidic technology allows the implementation of fast and sensitive detection of Sarin. In this paper, a micro-total analysis systems (TAS), also known as Lab-on-a-chip, fitted with an optical detection system has been developed to analyze the presence of the nerve agent sarin in water samples. In the present set-up, inhibition of co-introduced cholinesterase and water samples containing trace amounts of nerve agent sarin into the microfluidic device was used as the basis for selective detection of sarin. The device was fabricated using polymeric micromachining with PMMA (poly (methymethacrylate)) as the substrate material. A chromophore was utilized to measure the activity of remnant cholinesterase activity, which is inversely related to the amount of sarin present in the water samples. Comparisons were made between two different optical detection techniques and the findings will be presented in this paper. The presented measurement method is simple, fast and as sensitive as Gas Chromatography.
Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its... more Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its flexibility and low cost . Poly(methyl methacrylate) (PMMA) attracts growing interest in microfluidics research community due to its low cost, high transparency, good mechanical and chemical properties. Combining PMMA with PDMS allows a high degree of flexibility in designing microfluidic devices. Bonding of PMMA to PDMS is a critical step for this hybrid approach. We present here a novel technique for bonding PMMA to PDMS. This technique is simple, fast and reliable. A dry adhesive layer was laminated onto the PMMA substrate. A pre-cured PDMS mixture was spin coated onto the adhesive to form a PDMS membrane. The bonding quality and the strength of the PDMS/adhesive membrane was tested using a precision pressure source. The results show that the bond can hold a pressure up to 20 kPa.
The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can... more The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can act as cholinesterase inhibitors, that disrupts neuromuscular transmission. As these nerve agents are colorless, odorless and highly toxic, they can be introduced into drinking water as a means of terrorist sabotage. Hence, numerous innovative devices and methods have been developed for rapid detection of these organophosphates. Microfluidic technology allows the implementation of fast and sensitive detection of Sarin. In this paper, a micro-total analysis systems (TAS), also known as Lab-on-a-chip, fitted with an optical detection system has been developed to analyze the presence of the nerve agent sarin in water samples. In the present set-up, inhibition of co-introduced cholinesterase and water samples containing trace amounts of nerve agent sarin into the microfluidic device was used as the basis for selective detection of sarin. The device was fabricated using polymeric micromachining with PMMA (poly (methymethacrylate)) as the substrate material. A chromophore was utilized to measure the activity of remnant cholinesterase activity, which is inversely related to the amount of sarin present in the water samples. Comparisons were made between two different optical detection techniques and the findings will be presented in this paper. The presented measurement method is simple, fast and as sensitive as Gas Chromatography.
Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its... more Polydimethylsiloxane (PDMS) finds its use in a wide range of microfluidic applications due to its flexibility and low cost . Poly(methyl methacrylate) (PMMA) attracts growing interest in microfluidics research community due to its low cost, high transparency, good mechanical and chemical properties. Combining PMMA with PDMS allows a high degree of flexibility in designing microfluidic devices. Bonding of PMMA to PDMS is a critical step for this hybrid approach. We present here a novel technique for bonding PMMA to PDMS. This technique is simple, fast and reliable. A dry adhesive layer was laminated onto the PMMA substrate. A pre-cured PDMS mixture was spin coated onto the adhesive to form a PDMS membrane. The bonding quality and the strength of the PDMS/adhesive membrane was tested using a precision pressure source. The results show that the bond can hold a pressure up to 20 kPa.
The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can... more The chemical warfare agent Sarin is an organophosphate that is highly toxic to humans as they can act as cholinesterase inhibitors, that disrupts neuromuscular transmission. As these nerve agents are colorless, odorless and highly toxic, they can be introduced into drinking water as a means of terrorist sabotage. Hence, numerous innovative devices and methods have been developed for rapid detection of these organophosphates. Microfluidic technology allows the implementation of fast and sensitive detection of Sarin. In this paper, a micro-total analysis systems (TAS), also known as Lab-on-a-chip, fitted with an optical detection system has been developed to analyze the presence of the nerve agent sarin in water samples. In the present set-up, inhibition of co-introduced cholinesterase and water samples containing trace amounts of nerve agent sarin into the microfluidic device was used as the basis for selective detection of sarin. The device was fabricated using polymeric micromachining with PMMA (poly (methymethacrylate)) as the substrate material. A chromophore was utilized to measure the activity of remnant cholinesterase activity, which is inversely related to the amount of sarin present in the water samples. Comparisons were made between two different optical detection techniques and the findings will be presented in this paper. The presented measurement method is simple, fast and as sensitive as Gas Chromatography.
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