Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, 2015
Hand osteoarthritis (OA) is a very frequent disease, but yet understudied. However, a lot of work... more Hand osteoarthritis (OA) is a very frequent disease, but yet understudied. However, a lot of works have been published in the past 10 years, and much has been done to better understand its clinical course and structural progression. Despite this new knowledge, few therapeutic trials have been conducted in hand OA. The last OARSI recommendations for the conduct of clinical trials in hand OA dates back to 2006. The present recommendations aimed at updating previous recommendations, by incorporating new data. The purpose of this expert opinion, consensus driven exercise is to provide evidence-based guidance on the design, execution and analysis of clinical trials in hand OA, where published evidence is available, supplemented by expert opinion, where evidence is lacking, to perform clinical trials in hand OA, both for symptom and for structure-modification. They indicate core outcome measurement sets for studies in hand OA, and list the methods and instruments that should be used to me...
L’inflammation et l’angiogenese sont deux processus etroitement associes a la progression de l’ar... more L’inflammation et l’angiogenese sont deux processus etroitement associes a la progression de l’arthrose. Dans cette etude, nous avons compare la production de mediateurs pro-inflammatoires et angiogeniques par les synoviocytes de type fibroblastique isoles des zones enflammees (ZE) et non-enflammees (ZNE) de la membrane synoviale de genoux arthrosiques.
Background:Boswellia serrata extract (BSE) and curcumin are used to relief symptoms in osteoarthr... more Background:Boswellia serrata extract (BSE) and curcumin are used to relief symptoms in osteoarthritis (OA).Objectives:This study aims to better understand the mode of action of these compounds on OA chondrocytesin vitro.Methods:Therapeutic plasmatic concentrations of the different components of BSE correspond to anin vitrorange from 25 to 100 µg/ml of total BSE (100 µg/ml of BSE corresponds to 9.2 µM of 11-keto-β-boswellic acid (KBA), 5.2 µM of acetylKBA, 22 µM de αBA, 34 µM de βBA, 4.4 µM de acetylαBA and 13.2 acetyl βBA), and between 2 to 10 µM for bioavaibility-increased curcumin. BSE (5-100 µg/ml) and curcumin (0.04 to 4 µg/ml corresponding to 0.1 to 10 µM) were tested separately on primary chondrocytes from 3 OA patients. Lactate Deshydrogenase LDH, nitrite (NO2), interleukin (IL)-6 and Growth Differentiation Factor (GDF)15 were quantified in 72h-treated supernatant using enzyme activity, Griess reaction and ELISAs, respectively.Results:No mortality was observed at the tested c...
Objective: Avocado/soybean unsaponifiables (ASUs) are commonly used to treat OA symptoms. However... more Objective: Avocado/soybean unsaponifiables (ASUs) are commonly used to treat OA symptoms. However, there are many ASU mixtures on the market with differing compositions and pharmacological activities. This study aimed to compare the composition and pharmacological activity of seven commercially available ASU products on human osteoarthritis chondrocytes.Methods: The contents of the lipidic part of ASUs were characterized by gas chromatography analysis using a VARIAN 3400 chromatograph. The pharmacological activity of the ASU products was tested on human osteoarthritis chondrocytes cultured in alginate beads. Their effects were evaluated on aggrecan, interleukin (IL)-6 and -8, and matrix metalloproteases (MMP)-3 using immunoassays and on nitric oxide through measurement of nitrite via spectrometry.Results: PIASCLEDINE-ExpASU® showed a specific profile with the presence of chromatographic peaks corresponding to an alkyl furan fraction and alkyl triols. PIASCLEDINE-ExpASU®, Persemax, I...
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, 2015
Hand osteoarthritis (OA) is a very frequent disease, but yet understudied. However, a lot of work... more Hand osteoarthritis (OA) is a very frequent disease, but yet understudied. However, a lot of works have been published in the past 10 years, and much has been done to better understand its clinical course and structural progression. Despite this new knowledge, few therapeutic trials have been conducted in hand OA. The last OARSI recommendations for the conduct of clinical trials in hand OA dates back to 2006. The present recommendations aimed at updating previous recommendations, by incorporating new data. The purpose of this expert opinion, consensus driven exercise is to provide evidence-based guidance on the design, execution and analysis of clinical trials in hand OA, where published evidence is available, supplemented by expert opinion, where evidence is lacking, to perform clinical trials in hand OA, both for symptom and for structure-modification. They indicate core outcome measurement sets for studies in hand OA, and list the methods and instruments that should be used to me...
L’inflammation et l’angiogenese sont deux processus etroitement associes a la progression de l’ar... more L’inflammation et l’angiogenese sont deux processus etroitement associes a la progression de l’arthrose. Dans cette etude, nous avons compare la production de mediateurs pro-inflammatoires et angiogeniques par les synoviocytes de type fibroblastique isoles des zones enflammees (ZE) et non-enflammees (ZNE) de la membrane synoviale de genoux arthrosiques.
Background:Boswellia serrata extract (BSE) and curcumin are used to relief symptoms in osteoarthr... more Background:Boswellia serrata extract (BSE) and curcumin are used to relief symptoms in osteoarthritis (OA).Objectives:This study aims to better understand the mode of action of these compounds on OA chondrocytesin vitro.Methods:Therapeutic plasmatic concentrations of the different components of BSE correspond to anin vitrorange from 25 to 100 µg/ml of total BSE (100 µg/ml of BSE corresponds to 9.2 µM of 11-keto-β-boswellic acid (KBA), 5.2 µM of acetylKBA, 22 µM de αBA, 34 µM de βBA, 4.4 µM de acetylαBA and 13.2 acetyl βBA), and between 2 to 10 µM for bioavaibility-increased curcumin. BSE (5-100 µg/ml) and curcumin (0.04 to 4 µg/ml corresponding to 0.1 to 10 µM) were tested separately on primary chondrocytes from 3 OA patients. Lactate Deshydrogenase LDH, nitrite (NO2), interleukin (IL)-6 and Growth Differentiation Factor (GDF)15 were quantified in 72h-treated supernatant using enzyme activity, Griess reaction and ELISAs, respectively.Results:No mortality was observed at the tested c...
Objective: Avocado/soybean unsaponifiables (ASUs) are commonly used to treat OA symptoms. However... more Objective: Avocado/soybean unsaponifiables (ASUs) are commonly used to treat OA symptoms. However, there are many ASU mixtures on the market with differing compositions and pharmacological activities. This study aimed to compare the composition and pharmacological activity of seven commercially available ASU products on human osteoarthritis chondrocytes.Methods: The contents of the lipidic part of ASUs were characterized by gas chromatography analysis using a VARIAN 3400 chromatograph. The pharmacological activity of the ASU products was tested on human osteoarthritis chondrocytes cultured in alginate beads. Their effects were evaluated on aggrecan, interleukin (IL)-6 and -8, and matrix metalloproteases (MMP)-3 using immunoassays and on nitric oxide through measurement of nitrite via spectrometry.Results: PIASCLEDINE-ExpASU® showed a specific profile with the presence of chromatographic peaks corresponding to an alkyl furan fraction and alkyl triols. PIASCLEDINE-ExpASU®, Persemax, I...
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Papers by Yves Henrotin