X-ray fluorescence analysis of long-term changes in the levels and distributions of trace element... more X-ray fluorescence analysis of long-term changes in the levels and distributions of trace elements in the rat brain following mechanical injury
Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an inc... more Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an increased frequency of creatine inclusions in the rat hippocampal formation following pilocarpine-induced seizures
Epilepsy is one of the most common neurological disorders and despite of the longtime history of ... more Epilepsy is one of the most common neurological disorders and despite of the longtime history of researches under this disease the etiology of almost 70% of its cases is still unknown. The investigation under the pathogenesis of epilepsy are rarely carried out based on the human tissues, which can be obtained only post mortem or during the resection of epileptic foci. Therefore, different animal models of epileptic seizures are used. Epidemiological studies shows that the most frequently occurring type of epilepsy in adults is temporal lobe epilepsy (TLE) and the main features of TLE are [1]:
Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, 1999
Following a mechanical lesion of the left cerebral hemisphere, newborn male rats received a singl... more Following a mechanical lesion of the left cerebral hemisphere, newborn male rats received a single injection of recombinant rat interferon gamma (IFN gamma) into the lesion cavity at doses of 5, 50 and 500 U. One or two days after the injury the rats were injected with 3H-thymidine. Brain sections were immunostained for glial fibrillary acidic protein (GFAP), subjected to autoradiography and examined microscopically to record proliferating GFAP-immunopositive (GFAP+) astrocytes labeled with 3H-thymidine. Following the intermediate 50 U dose of IFN gamma, numbers of GFAP+ astrocytes and of their mitoses on day 1 after injury were significantly higher than in controls. Nevertheless, the astrocyte labeling index remained at the control level. Injections of the minimal 5 U or the maximal 500 U doses of IFN gamma had no effect on that day. On day 2, however, each of the three doses evoked a statistically significant but dose-independent reduction of the labeling index without similar cha...
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2014
Our previous studies carried out on the pilocarpine model of seizures showed that highly resolved... more Our previous studies carried out on the pilocarpine model of seizures showed that highly resolved elemental analysis might be very helpful in the investigation of processes involved in the pathogenesis of epilepsy, such as excitotoxicity or mossy fiber sprouting. In this study, the changes in elemental composition that occurred in the hippocampal formation in the electrical kindling model of seizures were examined to determine the mechanisms responsible for the phenomenon of kindling and spontaneous seizure activity that may occur in this animal model. X-ray fluorescence microscopy was applied for topographic and quantitative analysis of selected elements in tissues taken from rats subjected to repetitive transauricular electroshocks (ES) and controls (N). The detailed comparisons were carried out for sectors 1 and 3 of the Ammon's horn (CA1 and CA3, respectively), the dentate gyrus (DG) and hilus of DG. The obtained results showed only one statistically significant difference b...
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 2004
Wistar pregnant rats were exposed to a single 1.0 Gy dose of gamma rays on gestational days 13, 1... more Wistar pregnant rats were exposed to a single 1.0 Gy dose of gamma rays on gestational days 13, 15, 17 or 19 (E13, E15, E17 and E19, respectively). When offsprings of the irradiated females became 6-day-old, they received a mechanical injury of the cerebral hemisphere. One or 2 days after the injury, [3H]thymidine was injected and the animals were perfused. Brain sections were processed for BSI-B4 isolectin histochemistry or immunohistochemistry for glial fibrillary acidic protein (GFAP) or S-100-beta protein and subjected to autoradiography to visualise proliferating and non-proliferating macrophages or proliferating astrocytes. Significant changes in the contralateral response to injury related to the day of prenatal irradiation could be detected. The response was minimal following irradiations performed on E15 and E17. At those stages of prenatal development, the majority of cortical neurons with interhemispheric connections were formed. Therefore, irradiation-induced reduction o...
Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, 2000
Newborn male rats were subjected to a mechanical lesion of the left cerebral hemisphere. Thereaft... more Newborn male rats were subjected to a mechanical lesion of the left cerebral hemisphere. Thereafter, a single dose 5 or 500 units (U) of recombinant rat interleukin-1beta (IL-1beta) was injected into the lesion cavity. One or 2 days after the injury, the rats were injected with 3H-thymidine to label dividing cells. Brain sections were subjected to GFAP immunocytochemistry or BSI-B4 lectin histochemistry to visualise astrocytes or macrophages, respectively. Autoradiography was used to detect cells proliferating within the region of injury in the immunocytochemically stained brain sections. The strongest mitogenic effect of IL-1beta on astrocytes (labeling index) was observed on day 1 after injury while a dose-dependent increase in their GFAP-immunoreactivity occurred on day 2. At 500U dose, IL-1beta significantly reduced infiltration of macrophages on posttraumatic days 1 and 2 but did not influence their proliferation. Thus, effects of IL-1beta on the occurrence of macrophages were ...
Six-day-old male rats received a mechanical lesion in the left cerebral hemisphere. Thereafter, a... more Six-day-old male rats received a mechanical lesion in the left cerebral hemisphere. Thereafter, a single dose of either 5, 50 or 500 units (U) of recombinant rat interleukin-1 beta (IL-1 beta) was injected into the lesion cavity. One or 2 days after the injury, the rats were injected with 3H-thymidine. Brain sections were subjected to BSI-B4 lectin histochemistry and autoradiography to visualise proliferating and non-proliferating macrophages located within the region of injury. A mitogenic effect of IL-1 beta on macrophages was observed on day 2 in brains injected with the lowest 5 U dose of cytokine. Following administration of higher 50 U and 500 U doses, infiltration of the injured tissue by macrophages was significantly intensified on day 1. However, on day 2, dose-dependent reductions of the total number of macrophages as well as their proliferative activity were recorded. The findings suggest that the higher the initial quantity of macrophages, the sooner they disappeared fro...
X-ray fluorescence analysis of long-term changes in the levels and distributions of trace element... more X-ray fluorescence analysis of long-term changes in the levels and distributions of trace elements in the rat brain following mechanical injury
Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an inc... more Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an increased frequency of creatine inclusions in the rat hippocampal formation following pilocarpine-induced seizures
Epilepsy is one of the most common neurological disorders and despite of the longtime history of ... more Epilepsy is one of the most common neurological disorders and despite of the longtime history of researches under this disease the etiology of almost 70% of its cases is still unknown. The investigation under the pathogenesis of epilepsy are rarely carried out based on the human tissues, which can be obtained only post mortem or during the resection of epileptic foci. Therefore, different animal models of epileptic seizures are used. Epidemiological studies shows that the most frequently occurring type of epilepsy in adults is temporal lobe epilepsy (TLE) and the main features of TLE are [1]:
Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, 1999
Following a mechanical lesion of the left cerebral hemisphere, newborn male rats received a singl... more Following a mechanical lesion of the left cerebral hemisphere, newborn male rats received a single injection of recombinant rat interferon gamma (IFN gamma) into the lesion cavity at doses of 5, 50 and 500 U. One or two days after the injury the rats were injected with 3H-thymidine. Brain sections were immunostained for glial fibrillary acidic protein (GFAP), subjected to autoradiography and examined microscopically to record proliferating GFAP-immunopositive (GFAP+) astrocytes labeled with 3H-thymidine. Following the intermediate 50 U dose of IFN gamma, numbers of GFAP+ astrocytes and of their mitoses on day 1 after injury were significantly higher than in controls. Nevertheless, the astrocyte labeling index remained at the control level. Injections of the minimal 5 U or the maximal 500 U doses of IFN gamma had no effect on that day. On day 2, however, each of the three doses evoked a statistically significant but dose-independent reduction of the labeling index without similar cha...
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2014
Our previous studies carried out on the pilocarpine model of seizures showed that highly resolved... more Our previous studies carried out on the pilocarpine model of seizures showed that highly resolved elemental analysis might be very helpful in the investigation of processes involved in the pathogenesis of epilepsy, such as excitotoxicity or mossy fiber sprouting. In this study, the changes in elemental composition that occurred in the hippocampal formation in the electrical kindling model of seizures were examined to determine the mechanisms responsible for the phenomenon of kindling and spontaneous seizure activity that may occur in this animal model. X-ray fluorescence microscopy was applied for topographic and quantitative analysis of selected elements in tissues taken from rats subjected to repetitive transauricular electroshocks (ES) and controls (N). The detailed comparisons were carried out for sectors 1 and 3 of the Ammon's horn (CA1 and CA3, respectively), the dentate gyrus (DG) and hilus of DG. The obtained results showed only one statistically significant difference b...
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 2004
Wistar pregnant rats were exposed to a single 1.0 Gy dose of gamma rays on gestational days 13, 1... more Wistar pregnant rats were exposed to a single 1.0 Gy dose of gamma rays on gestational days 13, 15, 17 or 19 (E13, E15, E17 and E19, respectively). When offsprings of the irradiated females became 6-day-old, they received a mechanical injury of the cerebral hemisphere. One or 2 days after the injury, [3H]thymidine was injected and the animals were perfused. Brain sections were processed for BSI-B4 isolectin histochemistry or immunohistochemistry for glial fibrillary acidic protein (GFAP) or S-100-beta protein and subjected to autoradiography to visualise proliferating and non-proliferating macrophages or proliferating astrocytes. Significant changes in the contralateral response to injury related to the day of prenatal irradiation could be detected. The response was minimal following irradiations performed on E15 and E17. At those stages of prenatal development, the majority of cortical neurons with interhemispheric connections were formed. Therefore, irradiation-induced reduction o...
Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, 2000
Newborn male rats were subjected to a mechanical lesion of the left cerebral hemisphere. Thereaft... more Newborn male rats were subjected to a mechanical lesion of the left cerebral hemisphere. Thereafter, a single dose 5 or 500 units (U) of recombinant rat interleukin-1beta (IL-1beta) was injected into the lesion cavity. One or 2 days after the injury, the rats were injected with 3H-thymidine to label dividing cells. Brain sections were subjected to GFAP immunocytochemistry or BSI-B4 lectin histochemistry to visualise astrocytes or macrophages, respectively. Autoradiography was used to detect cells proliferating within the region of injury in the immunocytochemically stained brain sections. The strongest mitogenic effect of IL-1beta on astrocytes (labeling index) was observed on day 1 after injury while a dose-dependent increase in their GFAP-immunoreactivity occurred on day 2. At 500U dose, IL-1beta significantly reduced infiltration of macrophages on posttraumatic days 1 and 2 but did not influence their proliferation. Thus, effects of IL-1beta on the occurrence of macrophages were ...
Six-day-old male rats received a mechanical lesion in the left cerebral hemisphere. Thereafter, a... more Six-day-old male rats received a mechanical lesion in the left cerebral hemisphere. Thereafter, a single dose of either 5, 50 or 500 units (U) of recombinant rat interleukin-1 beta (IL-1 beta) was injected into the lesion cavity. One or 2 days after the injury, the rats were injected with 3H-thymidine. Brain sections were subjected to BSI-B4 lectin histochemistry and autoradiography to visualise proliferating and non-proliferating macrophages located within the region of injury. A mitogenic effect of IL-1 beta on macrophages was observed on day 2 in brains injected with the lowest 5 U dose of cytokine. Following administration of higher 50 U and 500 U doses, infiltration of the injured tissue by macrophages was significantly intensified on day 1. However, on day 2, dose-dependent reductions of the total number of macrophages as well as their proliferative activity were recorded. The findings suggest that the higher the initial quantity of macrophages, the sooner they disappeared fro...
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