Vaccinia virus encodes a homolog of the human complement regulators named vaccinia virus compleme... more Vaccinia virus encodes a homolog of the human complement regulators named vaccinia virus complement control protein (VCP). It is composed of four contiguous complement control protein (CCP) domains. Previously, VCP has been shown to bind to C3b and C4b and to inactivate the classical and alternative pathway C3 convertases by accelerating the decay of the classical pathway C3 convertase and (to a limited extent) the alternative pathway C3 convertase, as well as by supporting the factor I-mediated inactivation of C3b and C4b (the subunits of C3 convertases). In this study, we have mapped the CCP domains of VCP important for its cofactor activities, decay-accelerating activities, and binding to the target proteins by utilizing a series of deletion mutants. Our data indicate the following. (i) CCPs 1 to 3 are essential for cofactor activity for C3b and C4b; however, CCP 4 also contributes to the optimal activity. (ii) CCPs 1 to 2 are enough to mediate the classical pathway decay-acceler...
International Journal of Ayurveda and Pharma Research
Lifestyle in modern era delivers a lot of stress and due to extreme use of Guru (heavy), Madhura ... more Lifestyle in modern era delivers a lot of stress and due to extreme use of Guru (heavy), Madhura (sweet), Snigdha (oily), Shita (cold) Ahar and Vihar like Alashya (stationary), Adhyasana (taking another food before undigested food), Vishamashan (different timing of food) etc, people get suffer with a disease like excessive body fat or sick fat or adiposopathy. Adiposopathy leads to several metabolic diseases. Langhana upakrama (fasting) will be an effective treatment in adiposopathy. Several studies by researchers, assembled to check anatomical and pathophysiological abnormalities of adiposopathy and review of different aspects of Langhana upakrama in Samhita in perspective of metabolic disorders especially due to adiposopathy. Data study shows that on positive energy intake, accumulated subcutaneous adipose tissue, visceral adipose tissue and other fat depots can be pathogenic. This pathogenic adipose tissue causes many metabolic diseases like obesity, type 2 diabetes, hypertension...
A pyridine–pyrazole–pyrene-based multipurpose chemosensor has been introduced which has been foun... more A pyridine–pyrazole–pyrene-based multipurpose chemosensor has been introduced which has been found to exhibit excellent luminescent and optical properties.
A pyrene-based “turn-on” Cu(ii) sensor provides a chemiluminescent Cu6 metal organic macrocycle (... more A pyrene-based “turn-on” Cu(ii) sensor provides a chemiluminescent Cu6 metal organic macrocycle (MOM) applicable for live cell imaging.
The preservation of recurrent laryngeal nerve (RLN) is an essential part of thyroid surgery. The ... more The preservation of recurrent laryngeal nerve (RLN) is an essential part of thyroid surgery. The various methods to test the normal functionality of vocal cords (VCs) include direct visualisation under the fiber bronchoscope, indirect laryngoscopy, laryngeal muscles electromyography, computed tomography (CT), and magnetic resonance imaging (MRI). We aimed to assess the usefulness of ultrasound (USG) in the examination of VC morphology and movement. After Institutional Ethical Committee (IEC) clearance, 65 American Society of Anesthesiologists physical status I/II patients between the age group 18 and 60 years scheduled for thyroid surgery were enrolled in this observational study. All patients underwent USG examination before, immediately after and 2 days after thyroidectomy. The vocal fold (VF) displacement velocity (VFDV) was recorded and analysed. If any VF disorder was detected, the patients underwent two additional examinations: 2 and 3 months after thyroidectomy. All the findi...
Lignocellulose biorefinery encompasses process engineering and biotechnology tools for the proces... more Lignocellulose biorefinery encompasses process engineering and biotechnology tools for the processing of lignocellulosic biomass for the manufacturing of bio-based products (such as biofuels, bio-chemicals, biomaterials). While, lignocellulose biorefinery offers clear value proposition, success at industrial level has not been vibrant for the commercial production of renewable chemicals and fuels. This is because of high capital and operating expenditures, irregularities in biomass supply chain, technical process immaturity, and scale up challenges. As a result, commercial production of biochemicals and biofuels with right economics is still lagging behind. To hit the market place, efforts are underway by bulk and specialty chemicals producing companies like DSM (Succinic acid, Cellulosic ethanol), Dow-DuPont (1,3-Propanediol, 1,4-Butanediol), Clariant-Global bioenergies-INEOS (bio-isobutene), Braskem (Ethylene, polypropylene), Raizen, Gran-bio and POET-DSM (Cellulosic ethanol), Amy...
Autophagy, a regulated nutrient recycling program can affect both cell survival and cell death. H... more Autophagy, a regulated nutrient recycling program can affect both cell survival and cell death. Here, we show that Ormeloxifene (ORM), a selective estrogen receptor modulator approved for oral contraceptive use induces autophagic flux in ovarian cancer cells, which is activated by an ER stress response upstream of autophagy. The ER stress response is characterized by activation of IRE1α, PERK and ATF6 and is under regulation of JNK. Pharmacological inhibition of either autophagy or ER stress increased cell survival, as did silencing of autophagy proteins LC3 and Beclin 1, implying that ORM-induced autophagy is pro-death in nature. Ultrastructural observations of treated cells confirmed stages of autophagic maturation. Caspase-dependent apoptosis succeeded these events and was characterized by generation of reactive oxygen species and disruption of mitochondrial membrane potential. A concomitant inhibition of the Akt/mTOR axis was also observed with possible regulation of Akt by ORM....
Valence directed binding mode of the unsymmetrical ditopic ligand was observed in tetranuclear tr... more Valence directed binding mode of the unsymmetrical ditopic ligand was observed in tetranuclear trivalent (FeIII4) and mixed valent (FeII2FeIII2) [2 × 2] iron grid complexes.
Existing cancer therapies are often associated with drug resistance and toxicity, which results i... more Existing cancer therapies are often associated with drug resistance and toxicity, which results in poor prognosis and recurrence of cancer. This necessitates the identification and development of novel therapeutics against existing as well as novel cellular targets. In this study, a novel class of Benzocoumarin-Stilbene hybrid molecules were synthesized and evaluated for their antiproliferative activity against various cancer cell lines followed by in vivo antitumor activity in a mouse model of cancer. The most promising molecule among the series, i.e. compound (E)-4-(3,5-dimethoxystyryl)-2H-benzo[h]chromen-2-one (19) showed maximum antiproliferative activity in breast cancer cell lines (MDA-MB-231 and 4T1) and decreased the tumor size in the in-vivo 4T1 cell-induced orthotopic syngeneic mouse breast cancer model. The mechanistic studies of compound 19 by various biochemical, cell biology and biophysical approaches suggest that the compound binds to and inhibits the human DNA ligase...
The Journal of allergy and clinical immunology, Jan 16, 2017
Regulatory T cells play a critical role in preventing fetal organ-specific autoimmunity in humans... more Regulatory T cells play a critical role in preventing fetal organ-specific autoimmunity in humans. Autopsies of neonatal IPEX patients shortly after birth demonstrate chronic exocrine-dominant pancreatitis with tertiary lymphoid structures containing expanded oligoclonal T/B lymphocytes.
The Bi(3+)/Yb(3+)-codoped gadolinium tungstate phosphor has been synthesized through a solid-stat... more The Bi(3+)/Yb(3+)-codoped gadolinium tungstate phosphor has been synthesized through a solid-state reaction method. The structural characterization reveals the crystalline nature of the phosphor. The Bi(3+)-doped phosphor emits visible radiation from the blue to red regions upon excitation with 330 and 355 nm. The addition of Yb(3+) to the Bi(3+)-doped phosphor reduces the emission intensity in the visible region and emits an intense near-infrared (NIR) photon centered at 976 nm through a quantum-cutting (QC) phenomenon. This is due to cooperative energy transfer (CET) from the (3)P1 level of Bi(3+) to the (2)F5/2 level of Yb(3+). The presence of Li(+) ions in the Bi(3+)/Yb(3+)-codoped phosphor enhances the emission intensity in the NIR region up to by 3 times, whereas the emission intensity in the visible region is significantly reduced. The energy transfer (ET) from the Bi(3+) ions to the Yb(3+) ions is confirmed by lifetime measurements, and the lifetime for the (3)P1 level of Bi...
Vaccinia virus encodes a homolog of the human complement regulators named vaccinia virus compleme... more Vaccinia virus encodes a homolog of the human complement regulators named vaccinia virus complement control protein (VCP). It is composed of four contiguous complement control protein (CCP) domains. Previously, VCP has been shown to bind to C3b and C4b and to inactivate the classical and alternative pathway C3 convertases by accelerating the decay of the classical pathway C3 convertase and (to a limited extent) the alternative pathway C3 convertase, as well as by supporting the factor I-mediated inactivation of C3b and C4b (the subunits of C3 convertases). In this study, we have mapped the CCP domains of VCP important for its cofactor activities, decay-accelerating activities, and binding to the target proteins by utilizing a series of deletion mutants. Our data indicate the following. (i) CCPs 1 to 3 are essential for cofactor activity for C3b and C4b; however, CCP 4 also contributes to the optimal activity. (ii) CCPs 1 to 2 are enough to mediate the classical pathway decay-acceler...
International Journal of Ayurveda and Pharma Research
Lifestyle in modern era delivers a lot of stress and due to extreme use of Guru (heavy), Madhura ... more Lifestyle in modern era delivers a lot of stress and due to extreme use of Guru (heavy), Madhura (sweet), Snigdha (oily), Shita (cold) Ahar and Vihar like Alashya (stationary), Adhyasana (taking another food before undigested food), Vishamashan (different timing of food) etc, people get suffer with a disease like excessive body fat or sick fat or adiposopathy. Adiposopathy leads to several metabolic diseases. Langhana upakrama (fasting) will be an effective treatment in adiposopathy. Several studies by researchers, assembled to check anatomical and pathophysiological abnormalities of adiposopathy and review of different aspects of Langhana upakrama in Samhita in perspective of metabolic disorders especially due to adiposopathy. Data study shows that on positive energy intake, accumulated subcutaneous adipose tissue, visceral adipose tissue and other fat depots can be pathogenic. This pathogenic adipose tissue causes many metabolic diseases like obesity, type 2 diabetes, hypertension...
A pyridine–pyrazole–pyrene-based multipurpose chemosensor has been introduced which has been foun... more A pyridine–pyrazole–pyrene-based multipurpose chemosensor has been introduced which has been found to exhibit excellent luminescent and optical properties.
A pyrene-based “turn-on” Cu(ii) sensor provides a chemiluminescent Cu6 metal organic macrocycle (... more A pyrene-based “turn-on” Cu(ii) sensor provides a chemiluminescent Cu6 metal organic macrocycle (MOM) applicable for live cell imaging.
The preservation of recurrent laryngeal nerve (RLN) is an essential part of thyroid surgery. The ... more The preservation of recurrent laryngeal nerve (RLN) is an essential part of thyroid surgery. The various methods to test the normal functionality of vocal cords (VCs) include direct visualisation under the fiber bronchoscope, indirect laryngoscopy, laryngeal muscles electromyography, computed tomography (CT), and magnetic resonance imaging (MRI). We aimed to assess the usefulness of ultrasound (USG) in the examination of VC morphology and movement. After Institutional Ethical Committee (IEC) clearance, 65 American Society of Anesthesiologists physical status I/II patients between the age group 18 and 60 years scheduled for thyroid surgery were enrolled in this observational study. All patients underwent USG examination before, immediately after and 2 days after thyroidectomy. The vocal fold (VF) displacement velocity (VFDV) was recorded and analysed. If any VF disorder was detected, the patients underwent two additional examinations: 2 and 3 months after thyroidectomy. All the findi...
Lignocellulose biorefinery encompasses process engineering and biotechnology tools for the proces... more Lignocellulose biorefinery encompasses process engineering and biotechnology tools for the processing of lignocellulosic biomass for the manufacturing of bio-based products (such as biofuels, bio-chemicals, biomaterials). While, lignocellulose biorefinery offers clear value proposition, success at industrial level has not been vibrant for the commercial production of renewable chemicals and fuels. This is because of high capital and operating expenditures, irregularities in biomass supply chain, technical process immaturity, and scale up challenges. As a result, commercial production of biochemicals and biofuels with right economics is still lagging behind. To hit the market place, efforts are underway by bulk and specialty chemicals producing companies like DSM (Succinic acid, Cellulosic ethanol), Dow-DuPont (1,3-Propanediol, 1,4-Butanediol), Clariant-Global bioenergies-INEOS (bio-isobutene), Braskem (Ethylene, polypropylene), Raizen, Gran-bio and POET-DSM (Cellulosic ethanol), Amy...
Autophagy, a regulated nutrient recycling program can affect both cell survival and cell death. H... more Autophagy, a regulated nutrient recycling program can affect both cell survival and cell death. Here, we show that Ormeloxifene (ORM), a selective estrogen receptor modulator approved for oral contraceptive use induces autophagic flux in ovarian cancer cells, which is activated by an ER stress response upstream of autophagy. The ER stress response is characterized by activation of IRE1α, PERK and ATF6 and is under regulation of JNK. Pharmacological inhibition of either autophagy or ER stress increased cell survival, as did silencing of autophagy proteins LC3 and Beclin 1, implying that ORM-induced autophagy is pro-death in nature. Ultrastructural observations of treated cells confirmed stages of autophagic maturation. Caspase-dependent apoptosis succeeded these events and was characterized by generation of reactive oxygen species and disruption of mitochondrial membrane potential. A concomitant inhibition of the Akt/mTOR axis was also observed with possible regulation of Akt by ORM....
Valence directed binding mode of the unsymmetrical ditopic ligand was observed in tetranuclear tr... more Valence directed binding mode of the unsymmetrical ditopic ligand was observed in tetranuclear trivalent (FeIII4) and mixed valent (FeII2FeIII2) [2 × 2] iron grid complexes.
Existing cancer therapies are often associated with drug resistance and toxicity, which results i... more Existing cancer therapies are often associated with drug resistance and toxicity, which results in poor prognosis and recurrence of cancer. This necessitates the identification and development of novel therapeutics against existing as well as novel cellular targets. In this study, a novel class of Benzocoumarin-Stilbene hybrid molecules were synthesized and evaluated for their antiproliferative activity against various cancer cell lines followed by in vivo antitumor activity in a mouse model of cancer. The most promising molecule among the series, i.e. compound (E)-4-(3,5-dimethoxystyryl)-2H-benzo[h]chromen-2-one (19) showed maximum antiproliferative activity in breast cancer cell lines (MDA-MB-231 and 4T1) and decreased the tumor size in the in-vivo 4T1 cell-induced orthotopic syngeneic mouse breast cancer model. The mechanistic studies of compound 19 by various biochemical, cell biology and biophysical approaches suggest that the compound binds to and inhibits the human DNA ligase...
The Journal of allergy and clinical immunology, Jan 16, 2017
Regulatory T cells play a critical role in preventing fetal organ-specific autoimmunity in humans... more Regulatory T cells play a critical role in preventing fetal organ-specific autoimmunity in humans. Autopsies of neonatal IPEX patients shortly after birth demonstrate chronic exocrine-dominant pancreatitis with tertiary lymphoid structures containing expanded oligoclonal T/B lymphocytes.
The Bi(3+)/Yb(3+)-codoped gadolinium tungstate phosphor has been synthesized through a solid-stat... more The Bi(3+)/Yb(3+)-codoped gadolinium tungstate phosphor has been synthesized through a solid-state reaction method. The structural characterization reveals the crystalline nature of the phosphor. The Bi(3+)-doped phosphor emits visible radiation from the blue to red regions upon excitation with 330 and 355 nm. The addition of Yb(3+) to the Bi(3+)-doped phosphor reduces the emission intensity in the visible region and emits an intense near-infrared (NIR) photon centered at 976 nm through a quantum-cutting (QC) phenomenon. This is due to cooperative energy transfer (CET) from the (3)P1 level of Bi(3+) to the (2)F5/2 level of Yb(3+). The presence of Li(+) ions in the Bi(3+)/Yb(3+)-codoped phosphor enhances the emission intensity in the NIR region up to by 3 times, whereas the emission intensity in the visible region is significantly reduced. The energy transfer (ET) from the Bi(3+) ions to the Yb(3+) ions is confirmed by lifetime measurements, and the lifetime for the (3)P1 level of Bi...
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