Clitoria ternatea (L.) is a medicinal plant possessed with bioactive molecules such as taraxerol,... more Clitoria ternatea (L.) is a medicinal plant possessed with bioactive molecules such as taraxerol, delphinidin, kaempoferol and quercetin etc. For the development genotype with higher content of these bioactive molecules, marker-assisted breeding is one of the best strategies and it initiates with the development of F1 hybrids. Thus, an intraspecific F1 hybrid was raised involving two contrasting genotypes of C. ternatea acc. CtB3-SL1 (Blue flowered) and C. ternatea acc. CtW2-BL1 (white flowered). The hybridity of the F1 plant was confirmed by assessing the phenotypic traits, such as colour of the petal, pod shape and seed coat colour, 100 seed weight, and the content of taraxerol and delphinidin. The pollen mother cells in the F1 hybrid showed eight bivalents with preponderance of ring bivalents and 8I:8I segregation at metaphase-I and Anaphase-I, respectively. SDS-PAGE seed albumin and globulin detected three pollen parent-specific polypeptides (Mw 31.62, 22.38 and 18.81KDa), and w...
In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Ponga... more In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Pongamia pinnata (PLE) and characterized using various analytical techniques such as UV–visible spectroscopy, TEM, SEM, EDX, and XRD. UV–Visible spectra showed surface plasmon resonance (SPR) peaks in the range of 520–540 nm signifying the synthesis of colloidal AuNPs stabilised by PLE. Further, we investigated the cytotoxic effects of AuNPs against human cervical cancer cell line (HeLa). MTT assay screened IC50 value of 200 μg/mL at 24 h for AuNPs formed at varying HAuCl4 concentration. Flow cytometry measurements using H2DCFDA showed that the toxicity of AuNPs was attributed to the generation of reactive oxygen species (ROS). Besides, the in vitro anticancer activities of AuNPs were studied in HeLa cells by several assays which resulted in altered cell morphology, reduced potential of wound healing, inactive mitochondria due to loss of mitochondrial membrane potential (MMP) and cell cycle a...
Cajanus cajanifolius, the putative progenitor species of pigeonpea (C. cajan), has many agro-econ... more Cajanus cajanifolius, the putative progenitor species of pigeonpea (C. cajan), has many agro-economic traits that deserve attention for introgression into pigeonpea cultivars. However, unidirectional compatibility creates barriers for interspecific hybridization and alien gene transfer between these two species. In the present study, very low percentage of pod setting and F1 seed germination were observed. Thus, attempts were made for the propagation of interspecific F1 hybrids in vitro, and an efficient in vitro propagation protocol has been optimized. Excised cotyledonary explants of F1 hybrids were cultured on both MS and Blaydes’ medium supplemented with NAA, BAP and GA3 at various combinations and concentrations. MS medium with BAP (25 μM) was most suitable for multiple shoot induction. MS medium was found to be superior for both multiple shoot and root induction from cotyledonary explants. Cyto-morphological and SSR marker based analysis confirmed the hybridity of the micro-pr...
Clitoria ternatea (L.) is a medicinal leguminous plant and is cultivated to cater the need of her... more Clitoria ternatea (L.) is a medicinal leguminous plant and is cultivated to cater the need of herbal industries and asthetic purposes. The unavailability of steady molecular marker impedes the genetic improvement of C. ternatea. In the present study, transferability of 98 pairs of Cajanus spp. specific SSR primers were assessed among 14 genotypes of C. ternatea, varied for their flower color, floral architecture and bio-metabolite (taraxerol and delphinidin) content, and out of them 43 had successfully amplified the fragments. Among them, 36 pairs of primers showed 100% transferability, whereas rest seven varied from 42.86 to 92.85% transferability. The transferable 43 pairs of SSR primers generated 196 alleles across the 14 genotypes and the AMOVA analysis showed moderate genetic variation (55.1%) among the genotypes of C. ternatea, which was also reinforced by Nei's genetic distance and gene identity estimates derived haplotype matrix. Similarly, both the principal coordinate analysis and dendrogram grouped these 14 genotypes of C. ternatea into two major clusters based on SSR allele distribution and frequency, and the clustering pattern is in accordance with petal color but in contrast to floral architecture. MCheza based outlier analysis revealed 16 alleles for balancing selection, which are putatively involved in the maintenance of genetic polymorphism in C. ternatea. Moreover, the estimates of molecular diversity and bio-metabolite content revealed the possible use of these genotypes in future breeding programme of this species.
Clitoria ternatea (L.) is a medicinal plant possessed with bioactive molecules such as taraxerol,... more Clitoria ternatea (L.) is a medicinal plant possessed with bioactive molecules such as taraxerol, delphinidin, kaempoferol and quercetin etc. For the development genotype with higher content of these bioactive molecules, marker-assisted breeding is one of the best strategies and it initiates with the development of F1 hybrids. Thus, an intraspecific F1 hybrid was raised involving two contrasting genotypes of C. ternatea acc. CtB3-SL1 (Blue flowered) and C. ternatea acc. CtW2-BL1 (white flowered). The hybridity of the F1 plant was confirmed by assessing the phenotypic traits, such as colour of the petal, pod shape and seed coat colour, 100 seed weight, and the content of taraxerol and delphinidin. The pollen mother cells in the F1 hybrid showed eight bivalents with preponderance of ring bivalents and 8I:8I segregation at metaphase-I and Anaphase-I, respectively. SDS-PAGE seed albumin and globulin detected three pollen parent-specific polypeptides (Mw 31.62, 22.38 and 18.81KDa), and w...
In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Ponga... more In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Pongamia pinnata (PLE) and characterized using various analytical techniques such as UV–visible spectroscopy, TEM, SEM, EDX, and XRD. UV–Visible spectra showed surface plasmon resonance (SPR) peaks in the range of 520–540 nm signifying the synthesis of colloidal AuNPs stabilised by PLE. Further, we investigated the cytotoxic effects of AuNPs against human cervical cancer cell line (HeLa). MTT assay screened IC50 value of 200 μg/mL at 24 h for AuNPs formed at varying HAuCl4 concentration. Flow cytometry measurements using H2DCFDA showed that the toxicity of AuNPs was attributed to the generation of reactive oxygen species (ROS). Besides, the in vitro anticancer activities of AuNPs were studied in HeLa cells by several assays which resulted in altered cell morphology, reduced potential of wound healing, inactive mitochondria due to loss of mitochondrial membrane potential (MMP) and cell cycle a...
Cajanus cajanifolius, the putative progenitor species of pigeonpea (C. cajan), has many agro-econ... more Cajanus cajanifolius, the putative progenitor species of pigeonpea (C. cajan), has many agro-economic traits that deserve attention for introgression into pigeonpea cultivars. However, unidirectional compatibility creates barriers for interspecific hybridization and alien gene transfer between these two species. In the present study, very low percentage of pod setting and F1 seed germination were observed. Thus, attempts were made for the propagation of interspecific F1 hybrids in vitro, and an efficient in vitro propagation protocol has been optimized. Excised cotyledonary explants of F1 hybrids were cultured on both MS and Blaydes’ medium supplemented with NAA, BAP and GA3 at various combinations and concentrations. MS medium with BAP (25 μM) was most suitable for multiple shoot induction. MS medium was found to be superior for both multiple shoot and root induction from cotyledonary explants. Cyto-morphological and SSR marker based analysis confirmed the hybridity of the micro-pr...
Clitoria ternatea (L.) is a medicinal leguminous plant and is cultivated to cater the need of her... more Clitoria ternatea (L.) is a medicinal leguminous plant and is cultivated to cater the need of herbal industries and asthetic purposes. The unavailability of steady molecular marker impedes the genetic improvement of C. ternatea. In the present study, transferability of 98 pairs of Cajanus spp. specific SSR primers were assessed among 14 genotypes of C. ternatea, varied for their flower color, floral architecture and bio-metabolite (taraxerol and delphinidin) content, and out of them 43 had successfully amplified the fragments. Among them, 36 pairs of primers showed 100% transferability, whereas rest seven varied from 42.86 to 92.85% transferability. The transferable 43 pairs of SSR primers generated 196 alleles across the 14 genotypes and the AMOVA analysis showed moderate genetic variation (55.1%) among the genotypes of C. ternatea, which was also reinforced by Nei's genetic distance and gene identity estimates derived haplotype matrix. Similarly, both the principal coordinate analysis and dendrogram grouped these 14 genotypes of C. ternatea into two major clusters based on SSR allele distribution and frequency, and the clustering pattern is in accordance with petal color but in contrast to floral architecture. MCheza based outlier analysis revealed 16 alleles for balancing selection, which are putatively involved in the maintenance of genetic polymorphism in C. ternatea. Moreover, the estimates of molecular diversity and bio-metabolite content revealed the possible use of these genotypes in future breeding programme of this species.
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