Background: R. vitalii causes a remerging tick-borne disease known as rangeliosis. The parasite i... more Background: R. vitalii causes a remerging tick-borne disease known as rangeliosis. The parasite is not always identifable in blood smears, especially in the chronic phase of the disease. Low parasitemia levels have been observed in cases of rangeliosis caused by natural infection, even in acute situations, while hyperparasitemia has been reported only in acuteexperimental infection. This paper describes an unusual case of acute natural R. vitalii infection with hyperparasitemia.Case: The dog (a 12-year-old male German Shepherd) had presented apathy, dyschezia and hyporexia for three days prior to seeing the veterinarian, whose examination revealed discrete pale mucous membranes, soft bloody stools, hyperthermia, splenomegaly and lethargy. Numerous intra-erythrocytic forms, as well as free-living parasites compatible with R. vitalii and/or Babesia sp, were also found in the CBC (complete blood count), and the parasite load was estimated at 12 parasites/feld – x1000. After diagnosing ...
A micobacteriose e causada por bacilos alcool-acido resistentes do genero Mycobacterium . E incom... more A micobacteriose e causada por bacilos alcool-acido resistentes do genero Mycobacterium . E incomum em caes e gatos devido a sua maior resistencia a esses microrganismos. Este trabalho tem por objetivo descrever o caso de micobacteriose tegumentar em um cao, de ambiente urbano, bem como detalhar os aspectos citologicos e alertar os clinicos de pequenos animais para infeccoes por bacterias desse genero, mesmo em caes urbanos. Um cao, macho, da raca Teckel, de sete anos de idade, foi atendido por apresentar um nodulo na orelha direita. O animal raramente apresentava prurido nas orelhas e nao possuia historico de otite. Inicialmente, suspeitou-se de neoplasia cutânea. Portanto, foram solicitados hemograma, bioquimica serica (creatinina e alanina aminotransferase) e citologia aspirativa por agulha fina (CAAF). O paciente nao demonstrou nenhuma alteracao dos parâmetros fisiologicos durante o exame fisico e nem nos exames laboratoriais. A CAAF da orelha direita revelou a presenca de hemac...
O tamandua-bandeira ( Myrmecophaga tridactyla ) e uma especie vulneravel devido a destruicao prog... more O tamandua-bandeira ( Myrmecophaga tridactyla ) e uma especie vulneravel devido a destruicao progressiva do seu habitat natural, sendo afetado por queimadas e atropelamentos. O aumento na casuistica de atendimentos de animais silvestres reforca a necessidade do conhecimento dos parâmetros hematologicos, em especial para os que vivem no bioma do cerrado. Por isso, este trabalho teve por objetivos realizar o hemograma de tamanduas-bandeiras de cativeiro localizados no cerrado brasileiro, a fim de estabelecer valores de referencia para essa especie e compara-los a estudos previos. Para tanto, o sangue foi colhido por venopuncao da jugular e transferido para tubos contendo o anticoagulante acido etilenodiamino tetra-acetico (EDTA). A contagem de celulas foi realizada de forma manual, por meio de macrodiluicao em solucao diluente especifica, com o auxilio da câmara de Neubauer. Utilizou-se solucao de Gower para as hemacias, solucao de Turk para os leucocitos, e solucao de Brecher para as...
Os diferentes tempos em que as aves sao submetidas nos ambientes de espera nos abatedouros propic... more Os diferentes tempos em que as aves sao submetidas nos ambientes de espera nos abatedouros propiciam alteracoes no metabolismo animal. Este trabalho objetivou a avaliacao dos efeitos dos diferentes tempos de espera no abatedouro e a posicao das caixas de transporte no caminhao sobre o eritrograma e o leucograma de frangos de corte. Os tempos de espera foram de 0, 2, 4 e 6 horas, e as posicoes das caixas foram a superior e a inferior da fileira central do caminhao. Para o processo de amostragem, os frangos foram retirados das caixas de transporte apos os periodos de espera e realizado a colheita de sangue pela veia braquial. Imediatamente apos, foi realizado o hemograma por tecnica manual. A contagem das celulas foi feita em câmara de Neubauer, conforme o metodo de Natt e Herrick. Para visualizacao da morfologia celular e diferenciacao de leucocitos, foi feita a contagem em 100 celulas coradas com panotico rapido (Laborclin®, Pinhais/PR), utilizando microscopia de luz. Desta analise,...
O tamandua-bandeira (Myrmecophaga tridactyla), mamifero silvestre pertencente a superordem dos Xe... more O tamandua-bandeira (Myrmecophaga tridactyla), mamifero silvestre pertencente a superordem dos Xenarthras, e originario das Americas Central e do Sul. Atualmente, esta classificado como especie vulneravel, devido principalmente ao desmatamento, queimadas e atropelamento. A ausencia de valores de referencia para exames laboratoriais dificulta a avaliacao desses animais, especialmente devido ao aumento na casuistica de atendimento dessa especie em instituicoes veterinarias. Dada a importância de tais dados, objetivou-se com este estudo estabelecer parâmetros hematologicos, bioquimicos e eletroforeticos para tamanduas-bandeiras de cativeiro do cerrado, bem como, saber quais os efeitos de estocagem nas amostras hematologicas nessa especie em duas concentracoes distintas de anticoagulante. Para isso, foram utilizados 12 animais de cativeiro clinicamente saudaveis. O sangue foi colhido da jugular e armazenado em tubos apropriados para cada tipo de exame. Os hemogramas foram realizados log...
Background: Due to their abundant vascular supply, the lungs are a usual metastatic site, with pr... more Background: Due to their abundant vascular supply, the lungs are a usual metastatic site, with primary lung cancer presenting a low prevalence in dogs and cats. Among the primary pulmonary tumors afflicting dogs, lepidic carcinoma is the most common and can be classified by site of onset. Lepidic predominant adenocarcinoma is characterized by the proliferation of neoplastic cells along the pulmonary alveoli and pre-existing alveolar structures. Few reports of this specific tumor were found in the literature, so the objective of this study is to describe the clinical, pathological, and immunohistochemical findings of a dog with predominantly diffuse lepidic adenocarcinoma. Case: A 14-year-old, female, spayed, mixed-breed canine with a history of progressive weight loss, cough, respiratory distress, and pain while eating was brought in for a consultation. A presumptive diagnosis of pneumonia was suggested. However, in spite of treatment, the dog died, and a necropsy was performed. Upo...
ABSTRACT: This study aimed to evaluate and compare the effects of ozonized solutions on tissue wo... more ABSTRACT: This study aimed to evaluate and compare the effects of ozonized solutions on tissue wound repair in rats. Treatments consisted of ozonized water (GA), 0.9% sodium chloride (GCL), ozonized oil (GO), and 0.2% allantoin cream (GAL). The morphometric evaluation showed that wounds of the GA group presented a higher degree of retraction (p<0.05) at three and eight days of treatment (37.96 and 84.81%, respectively). Picrosirius red staining showed that groups GA and GO presented higher deposition (p<0.05) of type I collagen at 15 and 22 days of treatment, respectively. The neovascularization was higher in wounds of group GO on days 3, 8, and 15 (p<0.05), with higher VEGF immunostaining. (p<0.05). Thus, ozonized water enhances wound retraction and assists in the maturation and remodeling phase, while ozonized oil promotes higher neovascularization during tissue repair and higher deposition of type I collagen from the third week of treatment.
ABSTRACT: Giant anteater (Myrmecophaga tridactyla) is a wild mammal distributed in Central and So... more ABSTRACT: Giant anteater (Myrmecophaga tridactyla) is a wild mammal distributed in Central and South America; nowadays, it is classified as an endangered species. Research about the macroscopic and histomorphological aspects of its respiratory tract is scarce, and, sometimes, it limits the treatment provided to sick animals and impairs species preservation. Thus, the present study aims to describe the macroscopic and microscopic morphology of its lower respiratory tract, including trachea and lungs. To do so, 12 adult giant anteaters from “Centro de Triagem de Animais Silvestres de Goiânia” (CETAS-GO), Goiás State, Brazil, were used in the research after natural death or euthanasia. Three of these animals were used for macroscopic assessments; they were fixed in 10% buffered formalin and dissected. Trachea and lung tissue samples were collected from nine animals right after death and fixed in 10% buffered formalin for histomorphological analysis; they were processed, embedded in par...
There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition met... more There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition method or the ideal platelet concentration to stimulate tissue repair. Besides that, most studies do not provide a clear and replicable description of the methods used, which makes standardization and result comparison difficult. Thus, this study aimed to accurately describe the method and evaluate factors influencing PRP preparation in equines. In this regard, two protocols were used, P1 and P2, based on two centrifugation methods, which differed in blood volume, speed and time, and platelet pipetting and resuspension. In conclusion, factors such as centrifugation strength and time are essential to obtain PRP with adequate platelet count. However, other factors such as tube type and plasma pipetting and resuspension methods directly interfere with the replicability of the technique, and hence influencing PRP applicability.
ABSTRACT: The objective of this study was to evaluate the effect of digestible methionine and cys... more ABSTRACT: The objective of this study was to evaluate the effect of digestible methionine and cystine (Met + Cys) levels on the hematological and serum biochemical parameters of broiler chickens during the initial and growth stages. For this, 1,800 male chicks of the Coob 500 strain were used, with 900 chicks in the initial phase (1 to 21 days old) and 900 chicks in the growth phase (22 to 42 days old), distributed in a completely randomized design of five treatments with six replicates of 30 birds. The treatments consisted of 0.545, 0.616, 0.711, 0.782, and 0.853%; and 0.514, 0.571, 0.647, 0.704, and 0.761% digestible Met + Cys for “1 to 21” and “22 to 42” days of breeding, respectively. Results showed that digestible Met + Cys levels in broiler feed altered some hematological parameters (erythrocyte, hematocrit hemoglobin, total leukocytes, heterophile: lymphocyte) and serum biochemistry (uric acid, PST, total LDL, and TG). The digestible Met + Cys levels in the diet of broilers a...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2017
An investigation was made into the effects of different lairage times and the position of chicken... more An investigation was made into the effects of different lairage times and the position of chicken crates during transport to the slaughterhouse on the biochemical and hematological profile and physical parameters of broilers, such as color and pH of their breast meat. The treatments were defined by the animals slaughtered after 0, 2, 4 and 6 hours of lairage time at the slaughterhouse, transported in crates located in the top and bottom layers of the truck. It was found that increasing the lairage time at the slaughterhouse to over two hours reduced the number of lymphocytes and increased the heterophil/lymphocyte (H/L) ratio and the basophil count in the hemogram. In addition, lactate dehydrogenase (LDH) activity and cholesterol levels increased and plasma triglyceride and glucose levels decreased. The position of the crates in the truck altered the creatine kinase (CK) activity, and the highest enzyme activity was found in birds transported in the top layer of crates in the truck....
ABSTRACT: The giant anteater (Myrmecophaga tridactyla) is classified as a vulnerable species on B... more ABSTRACT: The giant anteater (Myrmecophaga tridactyla) is classified as a vulnerable species on Brazil’s list of species at risk of extinction mainly due to deforestation and forest fires. This has contributed to a considerable increase in detailed clinical case records of the treatment of wild species at veterinary institutions. However, the paucity of serum biochemical profiles of healthy giant anteaters has made it difficult to evaluate these animals, preventing diagnosis, treatment and prognosis. The objective of this work was to collect data about the biochemical profile of healthy giant anteaters from the Brazilian Cerrado raised in captivity, in order to better understand the physiological characteristics inherent to this species. Eighteen analytes from 12 healthy giant anteaters were measured. The following means and standard deviations were found in the biochemical analyses: albumin 3.29±0.33g/dL, ALT 15.49±7.98 IU/L, amylase 1037.92±149.04 IU/L, AST 21, 12±7.50 IU/L, total...
ABSTRACT: Giant Anteater (Myrmecophaga tridactyla) is a vulnerable species because of progressive... more ABSTRACT: Giant Anteater (Myrmecophaga tridactyla) is a vulnerable species because of progressive habitat destruction, mostly affected by wildfires and car accidents. The increasing number of animals that are attended by wildlife rescue centres reinforces the need of knowledge about haematological parameters, especially for those that inhabit Brazilian cerrado biome. For this purpose and in order to establish reference values for this species and also to compare them with previous studies, haematological analysis of captive giant anteaters from Brazilian cerrado were performed. Moreover, the alterations of blood samples after 24 and 48 hours of storage at refrigeration temperatures (4oC) and preserved with two different EDTA concentrations (5% and 10%) were studied. Means and standard deviations of haematological parameters analysed immediately after collection were: RBC: 2,07 x106/μL ± 0,40; hematocrit: 38,08%± 5,93; haemoglobin: 11.33g/dL±2.15; MCV:186.52 fL±21.72; MCHC: 29.68g/dL...
Background: R. vitalii causes a remerging tick-borne disease known as rangeliosis. The parasite i... more Background: R. vitalii causes a remerging tick-borne disease known as rangeliosis. The parasite is not always identifable in blood smears, especially in the chronic phase of the disease. Low parasitemia levels have been observed in cases of rangeliosis caused by natural infection, even in acute situations, while hyperparasitemia has been reported only in acuteexperimental infection. This paper describes an unusual case of acute natural R. vitalii infection with hyperparasitemia.Case: The dog (a 12-year-old male German Shepherd) had presented apathy, dyschezia and hyporexia for three days prior to seeing the veterinarian, whose examination revealed discrete pale mucous membranes, soft bloody stools, hyperthermia, splenomegaly and lethargy. Numerous intra-erythrocytic forms, as well as free-living parasites compatible with R. vitalii and/or Babesia sp, were also found in the CBC (complete blood count), and the parasite load was estimated at 12 parasites/feld – x1000. After diagnosing ...
A micobacteriose e causada por bacilos alcool-acido resistentes do genero Mycobacterium . E incom... more A micobacteriose e causada por bacilos alcool-acido resistentes do genero Mycobacterium . E incomum em caes e gatos devido a sua maior resistencia a esses microrganismos. Este trabalho tem por objetivo descrever o caso de micobacteriose tegumentar em um cao, de ambiente urbano, bem como detalhar os aspectos citologicos e alertar os clinicos de pequenos animais para infeccoes por bacterias desse genero, mesmo em caes urbanos. Um cao, macho, da raca Teckel, de sete anos de idade, foi atendido por apresentar um nodulo na orelha direita. O animal raramente apresentava prurido nas orelhas e nao possuia historico de otite. Inicialmente, suspeitou-se de neoplasia cutânea. Portanto, foram solicitados hemograma, bioquimica serica (creatinina e alanina aminotransferase) e citologia aspirativa por agulha fina (CAAF). O paciente nao demonstrou nenhuma alteracao dos parâmetros fisiologicos durante o exame fisico e nem nos exames laboratoriais. A CAAF da orelha direita revelou a presenca de hemac...
O tamandua-bandeira ( Myrmecophaga tridactyla ) e uma especie vulneravel devido a destruicao prog... more O tamandua-bandeira ( Myrmecophaga tridactyla ) e uma especie vulneravel devido a destruicao progressiva do seu habitat natural, sendo afetado por queimadas e atropelamentos. O aumento na casuistica de atendimentos de animais silvestres reforca a necessidade do conhecimento dos parâmetros hematologicos, em especial para os que vivem no bioma do cerrado. Por isso, este trabalho teve por objetivos realizar o hemograma de tamanduas-bandeiras de cativeiro localizados no cerrado brasileiro, a fim de estabelecer valores de referencia para essa especie e compara-los a estudos previos. Para tanto, o sangue foi colhido por venopuncao da jugular e transferido para tubos contendo o anticoagulante acido etilenodiamino tetra-acetico (EDTA). A contagem de celulas foi realizada de forma manual, por meio de macrodiluicao em solucao diluente especifica, com o auxilio da câmara de Neubauer. Utilizou-se solucao de Gower para as hemacias, solucao de Turk para os leucocitos, e solucao de Brecher para as...
Os diferentes tempos em que as aves sao submetidas nos ambientes de espera nos abatedouros propic... more Os diferentes tempos em que as aves sao submetidas nos ambientes de espera nos abatedouros propiciam alteracoes no metabolismo animal. Este trabalho objetivou a avaliacao dos efeitos dos diferentes tempos de espera no abatedouro e a posicao das caixas de transporte no caminhao sobre o eritrograma e o leucograma de frangos de corte. Os tempos de espera foram de 0, 2, 4 e 6 horas, e as posicoes das caixas foram a superior e a inferior da fileira central do caminhao. Para o processo de amostragem, os frangos foram retirados das caixas de transporte apos os periodos de espera e realizado a colheita de sangue pela veia braquial. Imediatamente apos, foi realizado o hemograma por tecnica manual. A contagem das celulas foi feita em câmara de Neubauer, conforme o metodo de Natt e Herrick. Para visualizacao da morfologia celular e diferenciacao de leucocitos, foi feita a contagem em 100 celulas coradas com panotico rapido (Laborclin®, Pinhais/PR), utilizando microscopia de luz. Desta analise,...
O tamandua-bandeira (Myrmecophaga tridactyla), mamifero silvestre pertencente a superordem dos Xe... more O tamandua-bandeira (Myrmecophaga tridactyla), mamifero silvestre pertencente a superordem dos Xenarthras, e originario das Americas Central e do Sul. Atualmente, esta classificado como especie vulneravel, devido principalmente ao desmatamento, queimadas e atropelamento. A ausencia de valores de referencia para exames laboratoriais dificulta a avaliacao desses animais, especialmente devido ao aumento na casuistica de atendimento dessa especie em instituicoes veterinarias. Dada a importância de tais dados, objetivou-se com este estudo estabelecer parâmetros hematologicos, bioquimicos e eletroforeticos para tamanduas-bandeiras de cativeiro do cerrado, bem como, saber quais os efeitos de estocagem nas amostras hematologicas nessa especie em duas concentracoes distintas de anticoagulante. Para isso, foram utilizados 12 animais de cativeiro clinicamente saudaveis. O sangue foi colhido da jugular e armazenado em tubos apropriados para cada tipo de exame. Os hemogramas foram realizados log...
Background: Due to their abundant vascular supply, the lungs are a usual metastatic site, with pr... more Background: Due to their abundant vascular supply, the lungs are a usual metastatic site, with primary lung cancer presenting a low prevalence in dogs and cats. Among the primary pulmonary tumors afflicting dogs, lepidic carcinoma is the most common and can be classified by site of onset. Lepidic predominant adenocarcinoma is characterized by the proliferation of neoplastic cells along the pulmonary alveoli and pre-existing alveolar structures. Few reports of this specific tumor were found in the literature, so the objective of this study is to describe the clinical, pathological, and immunohistochemical findings of a dog with predominantly diffuse lepidic adenocarcinoma. Case: A 14-year-old, female, spayed, mixed-breed canine with a history of progressive weight loss, cough, respiratory distress, and pain while eating was brought in for a consultation. A presumptive diagnosis of pneumonia was suggested. However, in spite of treatment, the dog died, and a necropsy was performed. Upo...
ABSTRACT: This study aimed to evaluate and compare the effects of ozonized solutions on tissue wo... more ABSTRACT: This study aimed to evaluate and compare the effects of ozonized solutions on tissue wound repair in rats. Treatments consisted of ozonized water (GA), 0.9% sodium chloride (GCL), ozonized oil (GO), and 0.2% allantoin cream (GAL). The morphometric evaluation showed that wounds of the GA group presented a higher degree of retraction (p<0.05) at three and eight days of treatment (37.96 and 84.81%, respectively). Picrosirius red staining showed that groups GA and GO presented higher deposition (p<0.05) of type I collagen at 15 and 22 days of treatment, respectively. The neovascularization was higher in wounds of group GO on days 3, 8, and 15 (p<0.05), with higher VEGF immunostaining. (p<0.05). Thus, ozonized water enhances wound retraction and assists in the maturation and remodeling phase, while ozonized oil promotes higher neovascularization during tissue repair and higher deposition of type I collagen from the third week of treatment.
ABSTRACT: Giant anteater (Myrmecophaga tridactyla) is a wild mammal distributed in Central and So... more ABSTRACT: Giant anteater (Myrmecophaga tridactyla) is a wild mammal distributed in Central and South America; nowadays, it is classified as an endangered species. Research about the macroscopic and histomorphological aspects of its respiratory tract is scarce, and, sometimes, it limits the treatment provided to sick animals and impairs species preservation. Thus, the present study aims to describe the macroscopic and microscopic morphology of its lower respiratory tract, including trachea and lungs. To do so, 12 adult giant anteaters from “Centro de Triagem de Animais Silvestres de Goiânia” (CETAS-GO), Goiás State, Brazil, were used in the research after natural death or euthanasia. Three of these animals were used for macroscopic assessments; they were fixed in 10% buffered formalin and dissected. Trachea and lung tissue samples were collected from nine animals right after death and fixed in 10% buffered formalin for histomorphological analysis; they were processed, embedded in par...
There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition met... more There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition method or the ideal platelet concentration to stimulate tissue repair. Besides that, most studies do not provide a clear and replicable description of the methods used, which makes standardization and result comparison difficult. Thus, this study aimed to accurately describe the method and evaluate factors influencing PRP preparation in equines. In this regard, two protocols were used, P1 and P2, based on two centrifugation methods, which differed in blood volume, speed and time, and platelet pipetting and resuspension. In conclusion, factors such as centrifugation strength and time are essential to obtain PRP with adequate platelet count. However, other factors such as tube type and plasma pipetting and resuspension methods directly interfere with the replicability of the technique, and hence influencing PRP applicability.
ABSTRACT: The objective of this study was to evaluate the effect of digestible methionine and cys... more ABSTRACT: The objective of this study was to evaluate the effect of digestible methionine and cystine (Met + Cys) levels on the hematological and serum biochemical parameters of broiler chickens during the initial and growth stages. For this, 1,800 male chicks of the Coob 500 strain were used, with 900 chicks in the initial phase (1 to 21 days old) and 900 chicks in the growth phase (22 to 42 days old), distributed in a completely randomized design of five treatments with six replicates of 30 birds. The treatments consisted of 0.545, 0.616, 0.711, 0.782, and 0.853%; and 0.514, 0.571, 0.647, 0.704, and 0.761% digestible Met + Cys for “1 to 21” and “22 to 42” days of breeding, respectively. Results showed that digestible Met + Cys levels in broiler feed altered some hematological parameters (erythrocyte, hematocrit hemoglobin, total leukocytes, heterophile: lymphocyte) and serum biochemistry (uric acid, PST, total LDL, and TG). The digestible Met + Cys levels in the diet of broilers a...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2017
An investigation was made into the effects of different lairage times and the position of chicken... more An investigation was made into the effects of different lairage times and the position of chicken crates during transport to the slaughterhouse on the biochemical and hematological profile and physical parameters of broilers, such as color and pH of their breast meat. The treatments were defined by the animals slaughtered after 0, 2, 4 and 6 hours of lairage time at the slaughterhouse, transported in crates located in the top and bottom layers of the truck. It was found that increasing the lairage time at the slaughterhouse to over two hours reduced the number of lymphocytes and increased the heterophil/lymphocyte (H/L) ratio and the basophil count in the hemogram. In addition, lactate dehydrogenase (LDH) activity and cholesterol levels increased and plasma triglyceride and glucose levels decreased. The position of the crates in the truck altered the creatine kinase (CK) activity, and the highest enzyme activity was found in birds transported in the top layer of crates in the truck....
ABSTRACT: The giant anteater (Myrmecophaga tridactyla) is classified as a vulnerable species on B... more ABSTRACT: The giant anteater (Myrmecophaga tridactyla) is classified as a vulnerable species on Brazil’s list of species at risk of extinction mainly due to deforestation and forest fires. This has contributed to a considerable increase in detailed clinical case records of the treatment of wild species at veterinary institutions. However, the paucity of serum biochemical profiles of healthy giant anteaters has made it difficult to evaluate these animals, preventing diagnosis, treatment and prognosis. The objective of this work was to collect data about the biochemical profile of healthy giant anteaters from the Brazilian Cerrado raised in captivity, in order to better understand the physiological characteristics inherent to this species. Eighteen analytes from 12 healthy giant anteaters were measured. The following means and standard deviations were found in the biochemical analyses: albumin 3.29±0.33g/dL, ALT 15.49±7.98 IU/L, amylase 1037.92±149.04 IU/L, AST 21, 12±7.50 IU/L, total...
ABSTRACT: Giant Anteater (Myrmecophaga tridactyla) is a vulnerable species because of progressive... more ABSTRACT: Giant Anteater (Myrmecophaga tridactyla) is a vulnerable species because of progressive habitat destruction, mostly affected by wildfires and car accidents. The increasing number of animals that are attended by wildlife rescue centres reinforces the need of knowledge about haematological parameters, especially for those that inhabit Brazilian cerrado biome. For this purpose and in order to establish reference values for this species and also to compare them with previous studies, haematological analysis of captive giant anteaters from Brazilian cerrado were performed. Moreover, the alterations of blood samples after 24 and 48 hours of storage at refrigeration temperatures (4oC) and preserved with two different EDTA concentrations (5% and 10%) were studied. Means and standard deviations of haematological parameters analysed immediately after collection were: RBC: 2,07 x106/μL ± 0,40; hematocrit: 38,08%± 5,93; haemoglobin: 11.33g/dL±2.15; MCV:186.52 fL±21.72; MCHC: 29.68g/dL...
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