Sample of leaf collected in Urama Carabobo State, Venezuela, was used to study and identify mycol... more Sample of leaf collected in Urama Carabobo State, Venezuela, was used to study and identify mycological on Melotria trilobata Cong., with the purpose to enter to the collection of the “Albert S. Muller” (VIA) mycological herbarium. Portions of leaves of the host were taken where superficial colonies were observed forming black reticular mycelium. Freehand cuts and prints with transparent tape were made, the tissues stained with lactofucsin and mounted on semipermanent slide. Measurements of the fungal structures were taken with a micrometric ocular coupled to a light microscope. Microscopic observations made it possible to observe mycelium of brown hyphae, sinuous, opposite ramification, with appressors alternating, capitate and unilateral, the basal cell cylindrical, subglobosa to ovate. Opposite phialides, ampuliform, dark brown, globular, scattered perithecia, peritecial setae, light brown, hooked and obtuse, 2 to 7 setae per perithecia, pale brown, ellipsoidal, 4 setae per perit...
In Venezuela there is little research done in relation to the microorganisms associated with the ... more In Venezuela there is little research done in relation to the microorganisms associated with the seeds of forest trees, microorganisms that can benefit or affect these seeds. Therefore, a sample of seeds of Caro (Enterolobium cyclocarpum (Jacq.) Griseb. The seeds were stored for two years in the Germplasm Bank of the National Center for the Conservation of Genetic Resources. A total of 300 seeds were analyzed in the Laboratory of Mycology of the Unit of Plant Protection of the Institute of Agricultural Research of INIA-CENIAP. The seeds were washed three times with sterile distilled water (EDW), separating them into two batches of 150 seeds each. The first batch was placed in trays with sterile absorbent paper and the second batch was seeded in culture medium of potato dextrose agar (PDA) supplemented with streptomycin sulfate. The seeds were incubated for eight days at 25(±) 3 °C and exposed to alternating cycles of 12 h of darkness and 12 h of light. Through the stereoscopic and o...
La mosca blanca Bemisia tabaci es un insecto senalado como un vector importante de virus de plant... more La mosca blanca Bemisia tabaci es un insecto senalado como un vector importante de virus de plantas a nivel mundial, con un amplio rango de plantas hospederas que incluyen ademas de plantas cultivadas, malezas, ornamentales, especies forestales y medicinales. El conocimiento sobre plantas hospederas de insectos plaga e insectos beneficos permite establecer planes de controles adaptados a las condiciones socioculturales y ambientales de la actividad agricola. En este sentido, el fin de este trabajo es contribuir al conocimiento de las plantas hospederas de B. tabaci en Venezuela, que permita proponer planes de manejo integrado de la mosca blanca. Para ello se reviso material bibliografico cientifico sobre mosca blanca, tomando como ano de partida, la publicacion de Arnal y Ramos del ano 2000, tambien se consideraron los reportes fitosanitarios de los laboratorios de Entomologia y Virologia de la Unidades de Proteccion Vegetal del INIA, asi como el de otras instituciones relacionadas ...
Identification of the causing agent of Stevia rebaudiana wilt in samples from Aragua State, Venez... more Identification of the causing agent of Stevia rebaudiana wilt in samples from Aragua State, Venezuela Two samples of Stevia rebaudiana, presenting upward necrotic wilt in the stem, were brought to the diagnostic service for phytopathogen-produced diseases of INIA-CENIAP, en Maracay, Venezuela. This species has been introduced in the country due to its potential as a sweetener. The pathogen was isolated by direct seeding, which consisted in separating each plant organ, disinfecting them with 2 % NaClO, and placed in potato dextrose agar culture medium (PDA, with streptomycin), under conditions of isolation chamber; tissue was incubated at 28 °C / 48 h. Then, typical colonies were isolated on PDA with streptomycin. After obtaining pure cultures, we proceeded with Koch's postulates. For inoculation 60-days old Stevia plants, grown in sterile substrate, were used. Plants were inoculated by infiltration using a suspension of 1x10 6 conidia of the pathogen per mL. Inoculated plants we...
ABSTRACT One hundred and ninety four type specimens held in the Mycological Herbarium “Albert S. ... more ABSTRACT One hundred and ninety four type specimens held in the Mycological Herbarium “Albert S. Muller” (VIA) are listed. Ninety eight relate to anamorphic fungi, 59 to Ascomycota, 36 to Basidiomycota and one to Oomycota.
Sample of leaf collected in Urama Carabobo State, Venezuela, was used to study and identify mycol... more Sample of leaf collected in Urama Carabobo State, Venezuela, was used to study and identify mycological on Melotria trilobata Cong., with the purpose to enter to the collection of the “Albert S. Muller” (VIA) mycological herbarium. Portions of leaves of the host were taken where superficial colonies were observed forming black reticular mycelium. Freehand cuts and prints with transparent tape were made, the tissues stained with lactofucsin and mounted on semipermanent slide. Measurements of the fungal structures were taken with a micrometric ocular coupled to a light microscope. Microscopic observations made it possible to observe mycelium of brown hyphae, sinuous, opposite ramification, with appressors alternating, capitate and unilateral, the basal cell cylindrical, subglobosa to ovate. Opposite phialides, ampuliform, dark brown, globular, scattered perithecia, peritecial setae, light brown, hooked and obtuse, 2 to 7 setae per perithecia, pale brown, ellipsoidal, 4 setae per perit...
In Venezuela there is little research done in relation to the microorganisms associated with the ... more In Venezuela there is little research done in relation to the microorganisms associated with the seeds of forest trees, microorganisms that can benefit or affect these seeds. Therefore, a sample of seeds of Caro (Enterolobium cyclocarpum (Jacq.) Griseb. The seeds were stored for two years in the Germplasm Bank of the National Center for the Conservation of Genetic Resources. A total of 300 seeds were analyzed in the Laboratory of Mycology of the Unit of Plant Protection of the Institute of Agricultural Research of INIA-CENIAP. The seeds were washed three times with sterile distilled water (EDW), separating them into two batches of 150 seeds each. The first batch was placed in trays with sterile absorbent paper and the second batch was seeded in culture medium of potato dextrose agar (PDA) supplemented with streptomycin sulfate. The seeds were incubated for eight days at 25(±) 3 °C and exposed to alternating cycles of 12 h of darkness and 12 h of light. Through the stereoscopic and o...
La mosca blanca Bemisia tabaci es un insecto senalado como un vector importante de virus de plant... more La mosca blanca Bemisia tabaci es un insecto senalado como un vector importante de virus de plantas a nivel mundial, con un amplio rango de plantas hospederas que incluyen ademas de plantas cultivadas, malezas, ornamentales, especies forestales y medicinales. El conocimiento sobre plantas hospederas de insectos plaga e insectos beneficos permite establecer planes de controles adaptados a las condiciones socioculturales y ambientales de la actividad agricola. En este sentido, el fin de este trabajo es contribuir al conocimiento de las plantas hospederas de B. tabaci en Venezuela, que permita proponer planes de manejo integrado de la mosca blanca. Para ello se reviso material bibliografico cientifico sobre mosca blanca, tomando como ano de partida, la publicacion de Arnal y Ramos del ano 2000, tambien se consideraron los reportes fitosanitarios de los laboratorios de Entomologia y Virologia de la Unidades de Proteccion Vegetal del INIA, asi como el de otras instituciones relacionadas ...
Identification of the causing agent of Stevia rebaudiana wilt in samples from Aragua State, Venez... more Identification of the causing agent of Stevia rebaudiana wilt in samples from Aragua State, Venezuela Two samples of Stevia rebaudiana, presenting upward necrotic wilt in the stem, were brought to the diagnostic service for phytopathogen-produced diseases of INIA-CENIAP, en Maracay, Venezuela. This species has been introduced in the country due to its potential as a sweetener. The pathogen was isolated by direct seeding, which consisted in separating each plant organ, disinfecting them with 2 % NaClO, and placed in potato dextrose agar culture medium (PDA, with streptomycin), under conditions of isolation chamber; tissue was incubated at 28 °C / 48 h. Then, typical colonies were isolated on PDA with streptomycin. After obtaining pure cultures, we proceeded with Koch's postulates. For inoculation 60-days old Stevia plants, grown in sterile substrate, were used. Plants were inoculated by infiltration using a suspension of 1x10 6 conidia of the pathogen per mL. Inoculated plants we...
ABSTRACT One hundred and ninety four type specimens held in the Mycological Herbarium “Albert S. ... more ABSTRACT One hundred and ninety four type specimens held in the Mycological Herbarium “Albert S. Muller” (VIA) are listed. Ninety eight relate to anamorphic fungi, 59 to Ascomycota, 36 to Basidiomycota and one to Oomycota.
Uploads
Papers by diego diamont