In this study, the effects of CO2 plasma surface modification and cross linking with collagen on ... more In this study, the effects of CO2 plasma surface modification and cross linking with collagen on the properties of poly-[3-hydroxybutyrate] (PHB) nanofibres were investigated. The samples were evaluated by attenuated total reflectance–Fourier transform infrared spectroscopy (ATR–FTIR), scanning electron microscope (SEM), contact angle and finally cell culture. ATR–FTIR structural and SEM analyses showed the presence of functional groups and coating on the nanofibrous surfaces. The 85° difference was obtained in the contact angle analysis, obtained for the collagen-cross-linked nanofibrous mat than the un-modified nanofibrous mat. Cellular investigation showed better adhesion of collagen-cross-linked nanofibrous samples than other samples. The collagen-cross-linked mats are a suitable scaffold for enhancing the cell adhesion in nerve regeneration.
International Journal of Polymeric Materials, Sep 18, 2015
ABSTRACT The aim of this study was to produce a nanofibrous biodegradable silk nerve substrate. T... more ABSTRACT The aim of this study was to produce a nanofibrous biodegradable silk nerve substrate. The woven and oriented artificial scaffolds were designed by electrospinning method, and were evaluated by microscopic, mechanical analyses, and cell culture assays with Schwann cells. Results of analyses were showed a good resilience and compliance with movement as a neural graft. Cellular experiments showed a better cell adhesion and growth on the oriented nanofibrous scaffolds compared to woven nanofiber ones. This neural conduit appears to have the right organization for testing in vivo nerve tissue engineering studies. GRAPHICAL ABSTRACT
The decellularization of plant tissues can be one of the design options of scaffolds in tissue en... more The decellularization of plant tissues can be one of the design options of scaffolds in tissue engineering. Chemical detergents such as Triton X‐100 and sodium dodecyl sulfate (SDS) in different concentrations were used to decellularize olive leaves as an acellular plant matrix for tissue engineering. The samples were investigated by different analyses such as Hematoxylin and Eosin staining, SEM, tensile strength, swelling, water vapor transmission, and toxicity. The results of staining and toxicity tests showed that the Triton X‐100 decellularized samples at a concentration of 0.1% had the best morphology and the lowest toxicity. Mechanical results showed that the elasticity modulus of acellular samples was significantly reduced compared to normal leaf samples. While swelling rate and water vapor transmission in acellular samples compared to the control sample doubled and tripled, respectively. In general, acellular olive leaf can be suggested as a scaffold for tissue engineering applications.
The antidepressant effects of aloe vera hydro alcoholic extract at different concentrations were ... more The antidepressant effects of aloe vera hydro alcoholic extract at different concentrations were compared with the fluoxetine-treated and the control groups of mice using forced-swimming, FST and open box, OFT tests. The mice were evaluated in five groups (control, taking aloe vera at the dosage levels of 150 mg/kg, 300 mg/kg, and 450 mg/kg, and finally fluoxetine at a dose of 10 mg/kg) by the FST and OFT tests on 1 st , 7 th , and 14 th days. The results of the OFT test showed no significant differences between these five groups. The results of FST test indicate the antidepressant effects of aloe vera even at low doses and it was found that the effect of fluoxetine at a dose of 10 mg/kg was equivalent to the effect of aloe vera at a dose of 150 mg/kg for the reduction in immobility time in mice in FST test. According to the results obtained from FST test, the antidepressant effects on mice treated with the 450 mg/kg dose of aloe vera showed better recovery as compared with other groups on 1 st , 7 th , and 14 th days. With regard to the experiments performed at different times, all the evidence pointed to the conclusion that the antidepressant effect of aloe vera was more than the control group. Based on the results of the OFT and FST tests, aloe vera extract at different doses, has favorable antidepressant effects on mice as compared to the fluoxetine-treated and the control groups and the better effects were seen by increasing the dose and duration of drug use.
Fish skin grafting as a new skin substitute is currently being used in clinical applications. Acc... more Fish skin grafting as a new skin substitute is currently being used in clinical applications. Acceleration of the wound healing, lack of disease transmission, and low cost of the production process can introduce fish skin as a potential alternative to other grafts. An appropriate decellularization process allows the design of 3D acellular scaffolds for skin regeneration without damaging the morphology and extracellular matrix content. Therefore, the role of decellularization processes is very important to maintain the properties of fish skin. In this review article, recent studies on various decellularization processes as well as biological, physical, and mechanical properties of fish skin and its applications with therapeutic effects in wound healing were investigated.
Hemorrhage control is vital for clinical outcomes after surgical treatment and pre-hospital traum... more Hemorrhage control is vital for clinical outcomes after surgical treatment and pre-hospital trauma injuries.
: Plant-derived tannic acid as a green material can play an important role in improving the mecha... more : Plant-derived tannic acid as a green material can play an important role in improving the mechanical and physical properties of biomaterials. Tannic acid can be used as an antioxidant, antimicrobial, and cross-linking agent in biomaterial products due to its unique functional groups. Its active phenolic groups can react with biomaterial functional groups to form bonds that improve performance. In this review, the mechanism of effectiveness of tannic acid as a natural crosslinker in improving the properties of biomaterials for various applications, such as tissue engineering, tissue adhesives, drug delivery, wound healing, and toxicity studies, has been investigated. In general, tannic acid can be a suitable alternative to synthetic crosslinkers in biomaterial applications.
Acellular skin as a scaffold has a good potential to regenerate or repair damaged tissues. Growth... more Acellular skin as a scaffold has a good potential to regenerate or repair damaged tissues. Growth factors such as Plasma Rich in Growth Factor (PRGF) as a rich source of active proteins can accelerate tissue regeneration. In this study, an acellular scaffold derived from fish skin with growth factors was used to repair full‐thickness skin defects in a rat model. Cellular results demonstrated that epithelial cells adhere well to acellular scaffolds. The results of animal studies showed that the groups treated with acellular scaffold and growth factor have a high ability to close and heal wounds on the 28th day after surgery. Histological and staining results showed that in the treated groups with scaffold and growth factor, an epidermal layer was formed with some skin appendages similar to normal skin. Overall, such scaffolds with biological agents can cause an acceptable synergistic effect on skin regeneration and wound healing.
The use of decellularized natural skin as an extracellular matrix (ECM) may be a great candidate ... more The use of decellularized natural skin as an extracellular matrix (ECM) may be a great candidate to regenerate damaged tissues. In this study, decellularized scaffolds from fish skin were designed by different techniques (physical, chemical, and enzymatic methods) and investigated by analyses such as Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM), Tensile strength, Degradability, Histological studies, Toxicity test, and Determination of DNA content. Results showed that the best sample is related to the decellularized skin by hypertonic & hypotonic technique and Triton X100 solutions. Structural and mechanical results were demonstrated that samples have similar properties to human skin to regenerate it. The cytotoxicity results showed that decellularized skin by hypertonic & hypotonic method and Triton solution is non-toxic with minimal amount of genetic materials. Cellular results with epithelial cells indicated good adhesion on decellularized matrix, so it can be a suitable candidate for skin tissue regeneration.
: For many years, discovering the appropriate methods for the treatment of skin irritation has be... more : For many years, discovering the appropriate methods for the treatment of skin irritation has been challenging for specialists and researchers. Bio-printing can be extensively applied to address the demand for proper skin substitutes to improve skin damage. Nowadays, to make more effective biomimicry of natural skin, many research teams have developed cell-seeded bio-inks for bioprinting of skin substitutes. These loaded cells can be single or co-cultured in these structures. The present review gives a comprehensive overview of the methods, substantial parameters of skin bioprinting, examples of in vitro and in vivo studies, and current advances and challenges in skin tissue engineering.
In this study, the effects of CO2 plasma surface modification and cross linking with collagen on ... more In this study, the effects of CO2 plasma surface modification and cross linking with collagen on the properties of poly-[3-hydroxybutyrate] (PHB) nanofibres were investigated. The samples were evaluated by attenuated total reflectance–Fourier transform infrared spectroscopy (ATR–FTIR), scanning electron microscope (SEM), contact angle and finally cell culture. ATR–FTIR structural and SEM analyses showed the presence of functional groups and coating on the nanofibrous surfaces. The 85° difference was obtained in the contact angle analysis, obtained for the collagen-cross-linked nanofibrous mat than the un-modified nanofibrous mat. Cellular investigation showed better adhesion of collagen-cross-linked nanofibrous samples than other samples. The collagen-cross-linked mats are a suitable scaffold for enhancing the cell adhesion in nerve regeneration.
International Journal of Polymeric Materials, Sep 18, 2015
ABSTRACT The aim of this study was to produce a nanofibrous biodegradable silk nerve substrate. T... more ABSTRACT The aim of this study was to produce a nanofibrous biodegradable silk nerve substrate. The woven and oriented artificial scaffolds were designed by electrospinning method, and were evaluated by microscopic, mechanical analyses, and cell culture assays with Schwann cells. Results of analyses were showed a good resilience and compliance with movement as a neural graft. Cellular experiments showed a better cell adhesion and growth on the oriented nanofibrous scaffolds compared to woven nanofiber ones. This neural conduit appears to have the right organization for testing in vivo nerve tissue engineering studies. GRAPHICAL ABSTRACT
The decellularization of plant tissues can be one of the design options of scaffolds in tissue en... more The decellularization of plant tissues can be one of the design options of scaffolds in tissue engineering. Chemical detergents such as Triton X‐100 and sodium dodecyl sulfate (SDS) in different concentrations were used to decellularize olive leaves as an acellular plant matrix for tissue engineering. The samples were investigated by different analyses such as Hematoxylin and Eosin staining, SEM, tensile strength, swelling, water vapor transmission, and toxicity. The results of staining and toxicity tests showed that the Triton X‐100 decellularized samples at a concentration of 0.1% had the best morphology and the lowest toxicity. Mechanical results showed that the elasticity modulus of acellular samples was significantly reduced compared to normal leaf samples. While swelling rate and water vapor transmission in acellular samples compared to the control sample doubled and tripled, respectively. In general, acellular olive leaf can be suggested as a scaffold for tissue engineering applications.
The antidepressant effects of aloe vera hydro alcoholic extract at different concentrations were ... more The antidepressant effects of aloe vera hydro alcoholic extract at different concentrations were compared with the fluoxetine-treated and the control groups of mice using forced-swimming, FST and open box, OFT tests. The mice were evaluated in five groups (control, taking aloe vera at the dosage levels of 150 mg/kg, 300 mg/kg, and 450 mg/kg, and finally fluoxetine at a dose of 10 mg/kg) by the FST and OFT tests on 1 st , 7 th , and 14 th days. The results of the OFT test showed no significant differences between these five groups. The results of FST test indicate the antidepressant effects of aloe vera even at low doses and it was found that the effect of fluoxetine at a dose of 10 mg/kg was equivalent to the effect of aloe vera at a dose of 150 mg/kg for the reduction in immobility time in mice in FST test. According to the results obtained from FST test, the antidepressant effects on mice treated with the 450 mg/kg dose of aloe vera showed better recovery as compared with other groups on 1 st , 7 th , and 14 th days. With regard to the experiments performed at different times, all the evidence pointed to the conclusion that the antidepressant effect of aloe vera was more than the control group. Based on the results of the OFT and FST tests, aloe vera extract at different doses, has favorable antidepressant effects on mice as compared to the fluoxetine-treated and the control groups and the better effects were seen by increasing the dose and duration of drug use.
Fish skin grafting as a new skin substitute is currently being used in clinical applications. Acc... more Fish skin grafting as a new skin substitute is currently being used in clinical applications. Acceleration of the wound healing, lack of disease transmission, and low cost of the production process can introduce fish skin as a potential alternative to other grafts. An appropriate decellularization process allows the design of 3D acellular scaffolds for skin regeneration without damaging the morphology and extracellular matrix content. Therefore, the role of decellularization processes is very important to maintain the properties of fish skin. In this review article, recent studies on various decellularization processes as well as biological, physical, and mechanical properties of fish skin and its applications with therapeutic effects in wound healing were investigated.
Hemorrhage control is vital for clinical outcomes after surgical treatment and pre-hospital traum... more Hemorrhage control is vital for clinical outcomes after surgical treatment and pre-hospital trauma injuries.
: Plant-derived tannic acid as a green material can play an important role in improving the mecha... more : Plant-derived tannic acid as a green material can play an important role in improving the mechanical and physical properties of biomaterials. Tannic acid can be used as an antioxidant, antimicrobial, and cross-linking agent in biomaterial products due to its unique functional groups. Its active phenolic groups can react with biomaterial functional groups to form bonds that improve performance. In this review, the mechanism of effectiveness of tannic acid as a natural crosslinker in improving the properties of biomaterials for various applications, such as tissue engineering, tissue adhesives, drug delivery, wound healing, and toxicity studies, has been investigated. In general, tannic acid can be a suitable alternative to synthetic crosslinkers in biomaterial applications.
Acellular skin as a scaffold has a good potential to regenerate or repair damaged tissues. Growth... more Acellular skin as a scaffold has a good potential to regenerate or repair damaged tissues. Growth factors such as Plasma Rich in Growth Factor (PRGF) as a rich source of active proteins can accelerate tissue regeneration. In this study, an acellular scaffold derived from fish skin with growth factors was used to repair full‐thickness skin defects in a rat model. Cellular results demonstrated that epithelial cells adhere well to acellular scaffolds. The results of animal studies showed that the groups treated with acellular scaffold and growth factor have a high ability to close and heal wounds on the 28th day after surgery. Histological and staining results showed that in the treated groups with scaffold and growth factor, an epidermal layer was formed with some skin appendages similar to normal skin. Overall, such scaffolds with biological agents can cause an acceptable synergistic effect on skin regeneration and wound healing.
The use of decellularized natural skin as an extracellular matrix (ECM) may be a great candidate ... more The use of decellularized natural skin as an extracellular matrix (ECM) may be a great candidate to regenerate damaged tissues. In this study, decellularized scaffolds from fish skin were designed by different techniques (physical, chemical, and enzymatic methods) and investigated by analyses such as Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM), Tensile strength, Degradability, Histological studies, Toxicity test, and Determination of DNA content. Results showed that the best sample is related to the decellularized skin by hypertonic & hypotonic technique and Triton X100 solutions. Structural and mechanical results were demonstrated that samples have similar properties to human skin to regenerate it. The cytotoxicity results showed that decellularized skin by hypertonic & hypotonic method and Triton solution is non-toxic with minimal amount of genetic materials. Cellular results with epithelial cells indicated good adhesion on decellularized matrix, so it can be a suitable candidate for skin tissue regeneration.
: For many years, discovering the appropriate methods for the treatment of skin irritation has be... more : For many years, discovering the appropriate methods for the treatment of skin irritation has been challenging for specialists and researchers. Bio-printing can be extensively applied to address the demand for proper skin substitutes to improve skin damage. Nowadays, to make more effective biomimicry of natural skin, many research teams have developed cell-seeded bio-inks for bioprinting of skin substitutes. These loaded cells can be single or co-cultured in these structures. The present review gives a comprehensive overview of the methods, substantial parameters of skin bioprinting, examples of in vitro and in vivo studies, and current advances and challenges in skin tissue engineering.
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