Gossypol, a polyphenolic compound which depletes cellular energy by inhibition of several intrace... more Gossypol, a polyphenolic compound which depletes cellular energy by inhibition of several intracellular dehydrogenases, has been shown to have antiproliferative activity against human glial tumor cell lines in vitro and in nude mouse xenografts. Human trials of gossypol as a male contraceptive have demonstrated safety of long-term administration. We studied the activity of Gossypol 10 mg PO bid in 27 patients with pathologically confirmed glial tumors which had recurred after radiation therapy. Fifteen patients had glioblastoma, 11 patients anaplastic astrocytoma, 1 patient relapsed low grade glioma. Response was assessed every 8 weeks using CT/MRI scan and clinical criteria including decadron requirement. Treatment was continued until disease progression. Two patients had partial response (PR); 4 had stable disease for 8 weeks or more. One patient maintained a PR with improved KPS for 78 weeks. The other had a PR lasting 8 weeks. Toxicity was mild: 2 heavily pretreated patients had mild thrombocytopenia, 5 patients developed hypokalemia, 3 patients developed grade 2 hepatic toxicity and peripheral edema. Gossypol levels measured by HPLC did not correlate with response or toxicity in this study. We conclude that gossypol is well tolerated and has a low, but measurable, response rate in a heavily pretreated, poor-prognosis group of patients with recurrent glioma. The presumed novel mechanism of action, lack of significant myelosuppression, and activity in patients with advance glioma support further study of gossypol as an antineoplastic agent.
It has been proposed that preeclampsia is a metabolic syndrome of pregnancy. The polymorphisms Ps... more It has been proposed that preeclampsia is a metabolic syndrome of pregnancy. The polymorphisms PstI and MaeIII of INS, NsiI of INSR and Ala513Pro and Gly972Arg of IRS1 have been associated with metabolic syndrome; moreover, the products of these genes are functionally contiguous during insulin signaling. The aim of this study was to assess whether these polymorphisms are associated with preeclampsia. 46 normotensive pregnant women and 43 preeclamptic patients were included in the study to develop a clinical, biochemical and genotypic profile of preeclampsia. Clinical evaluation consisted of measurement of blood pressure, height and weight. Peripheral blood samples were collected for determination of fasting glucose and insulin concentrations and for extraction of genomic DNA. Proteinuria was determined. Polymorphisms were detected using PCR-RFLP. The normotensive and preeclampsia groups did not differ significantly in clinical and biochemical traits, except for systolic and diastolic blood pressure (p < 0.0001). Polymorphisms previously associated with metabolic syndrome in Mexican populations were not associated with preeclampsia in Mexican women (p > 0.05). The lack of an association between preeclampsia and the polymorphisms studied suggests that other genes whose products do not have direct functional interaction with metabolic syndrome or epigenetic factors may play a role in preeclampsia.
Tree row inventories are of increasing interest because tree rows mitigate wind erosion and deser... more Tree row inventories are of increasing interest because tree rows mitigate wind erosion and desertification, protect agricultural crops, enhance rural landscape quality, act as bio-corridors, carbon sinks, and a source for bio-energy. The main objective of tree row inventories is to estimate population parameters such as total tree numbers, total tree numbers by species, the mean stem diameter at breast height, the mean tree height and total wood volume. The estimation of these quantities may be straightforwardly carried out whenever aerial images are available in such a way that tree rows can be counted: in these cases, a two-stage cluster sampling may be performed in which the primary units sampled in the first stage are the tree rows in the study area while the secondary units sampled in the second stage are the trees within the selected rows. This paper proposes two sets of two-stage estimators for the interest parameters, based on the Horvitz–Thompson and ratio criteria, together with the corresponding estimators for their sampling variances. The use of stratification is also considered. The proposed procedure was applied to perform a tree row inventory in the Pontina plain (Central Italy): in this case, the tree rows were enumerated by means of ortho-corrected airborne images and stratification was carried out on the basis of the prevailing species and age classes. The inventory results are interesting from a forestry perspective as well as for checking the effectiveness of the procedure.
To assess bone regeneration in critical sized defects in the rabbit calvarium, filled with the bo... more To assess bone regeneration in critical sized defects in the rabbit calvarium, filled with the bone substitute calcium phosphate cement. Circular bone defects (8mm) were made in both parietal bones of 10 rabbits. One of the defects was filled with the calcium phosphate cement, and the other received autogenous bone harvested from the calvaria. The animals were killed at 3 or 6 weeks (n=5). Data analysis included qualitative assessment of the calvarial specimens and histomorphometric analysis was used to quantify the amount of new bone within the defects. The microscopic analysis of the samples showed bone healing with both calcium phosphate cement and autogenous bone graft. Data obtained from the histomorphometric analysis were statistically analyzed using 2-way analysis of variance and the Tukey's test. Data analysis showed that the autogenous bone graft had significantly more new bone compared with calcium phosphate cement at 3 and 6 weeks. Calcium phosphate cement at 6 weeks presented similar results to autogenous bone at 3 weeks. Both treatments presented an increase in bone healing with time. Treatments allowed bone regeneration that increased with time, however surgical cavities treated with the autogenous graft had more bone formation than those with calcium phosphate cement.
... Publishing Operations Division Director: Vanessa Hayden Directors: Karen Adams-Taylor, Susana... more ... Publishing Operations Division Director: Vanessa Hayden Directors: Karen Adams-Taylor, Susana Eloy de Vitale, Ryan Patella, Sue Sherrill Managers: David Antos, Karen Branham, Suzanne J. Bukovsky, Debbie Camp, Susan Chiara, Diane Darnell, Karl Elvin, Mary Ellen ...
Urban areas are continuously expanding today, extending their influence on an increasingly large ... more Urban areas are continuously expanding today, extending their influence on an increasingly large proportion of woods and trees located in or nearby urban and urbanizing areas, the so-called urban forests. Although these forests have the potential for significantly improving the quality the urban environment and the well-being of the urban population, data to quantify the extent and characteristics of urban forests are still lacking or fragmentary on a large scale. In this regard, an expansion of the domain of multipurpose forest inventories like National Forest Inventories (NFIs) towards urban forests would be required. To this end, it would be convenient to exploit the same sampling scheme applied in NFIs to assess the basic features of urban forests. This paper considers approximately unbiased estimators of abundance and coverage of urban forests, together with estimators of the corresponding variances, which can be achieved from the first phase of most large-scale forest inventories. A simulation study is carried out in order to check the performance of the considered estimators under various situations involving the spatial distribution of the urban forests over the study area. An application is worked out on the data from the Italian NFI.
Canadian Journal of Forest Research-revue Canadienne De Recherche Forestiere, 2008
... Area-based lidar-assisted estimation of forest standing volume Piermaria Corona and Lorenzo F... more ... Area-based lidar-assisted estimation of forest standing volume Piermaria Corona and Lorenzo Fattorini ... P. Corona.1 Dipartimento di Scienze dell'Ambiente Forestale e delle sue Risorse, Universita` della Tuscia, via San Camillo de Lellis, 01100 Viterbo, Italy. L. Fattorini. ...
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2000
Helminths, particularly some Schistosoma species, have been associated with cancer in humans. Neu... more Helminths, particularly some Schistosoma species, have been associated with cancer in humans. Neurocysticercosis, produced by cysticerci of the helminth Taenia solium, has been associated with the emergence of brain tumours and haematological malignancies. Local tumours, such as glioblastoma, could be explained by the induction of DNA damage in cells surrounding the cysticercus and chronically exposed to an inflammatory host response. However, systemic effects such as haematological malignancies are not easy to understand. The present work was conducted in Mexico to find out whether DNA damage arises in peripheral lymphocytes in patients with neurocysticercosis. We utilized a highly sensitive technique to analyse chromosomal aberrations, in-situ hybridization with probes against chromosomes 1, 2 and 4, and in addition the blocked-cytokinesis technique was used to determine the formation of micronuclei, a peculiar form of DNA damage. The study was made in lymphocytes from 8 patients before and after the administration of praziquantel, 1 of the 2 drugs used for neurocysticercosis treatment. The frequencies of chromosome aberrations and micronuclei in peripheral blood lymphocytes were higher in the infected patients as compared to those observed both in healthy donors and in the group of patients after praziquantel therapy. Our results suggest that chromosome aberrations induced in peripheral cells during neurocysticercosis could be associated with the development of haematological neoplasias.
Forest surveys performed over a large scale (e.g. national inventories) involve several phases of... more Forest surveys performed over a large scale (e.g. national inventories) involve several phases of sampling. The first phase is usually performed by means of a systematic search of the study region, in which the region is partitioned into regular polygons of the same size and points are randomly or systematically selected, one per polygon. In most cases, first-phase points are selected and recognized in orthophotos or very high resolution satellite images available for the whole study area. Disregarding the subsequent phases, the first phase of sampling can be effectively adopted to select small woodlots and tree rows, in the sense that a unit is selected when at least one first-phase point falls within it. On the basis of such a scheme of sampling, approximately unbiased estimators of abundance, coverage and other physical attributes readily measurable from orthophotos (e.g. tree-row length) are proposed, together with estimators of the corresponding variances. A simulation study is performed in order to check the performance of the estimators under several distributions of units over the study area (random, clustered, spatially trended).
Forest area in the year 1990 is a figure of great interest under the Kyoto Protocol. This note is... more Forest area in the year 1990 is a figure of great interest under the Kyoto Protocol. This note is devoted to a scientific exercise for the probabilistic ex post assessment of such a figure in Italy. Estimation was performed by two-phase point sampling, which made use of historical remotely sensed imagery. In the first phase, a sample of 12 089 points was selected according to an unaligned systematic sampling and the selected points were classified in land-use categories by Landsat 5 TM imagery. In the second phase, a sample of 3000 points was selected by stratified sampling in which the strata were determined by the satellite classification and the selected points were classified by aerial photos, assumed as ground truth. A two-phase estimate of land-use coverage partitioning the Italian territory was obtained together with a conservative estimate of the sampling variance-covariance. The procedure has proved to be of relatively easy implementation and objective repeatability.
Journal of The American Academy of Dermatology, 2003
There is a need for better standardization of the dermoscopic terminology in assessing pigmented ... more There is a need for better standardization of the dermoscopic terminology in assessing pigmented skin lesions. The virtual Consensus Net Meeting on Dermoscopy was organized to investigate reproducibility and validity of the various features and diagnostic algorithms. Dermoscopic images of 108 lesions were evaluated via the Internet by 40 experienced dermoscopists using a 2-step diagnostic procedure. The first-step algorithm distinguished melanocytic versus nonmelanocytic lesions. The second step in the diagnostic procedure used 4 algorithms (pattern analysis, ABCD rule, Menzies method, and 7-point checklist) to distinguish melanoma versus benign melanocytic lesions. kappa Values, log odds ratios, sensitivity, specificity, and positive likelihood ratios were estimated for all diagnostic algorithms and dermoscopic features. Interobserver agreement was fair to good for all diagnostic methods, but it was poor for the majority of dermoscopic criteria. Intraobserver agreement was good to excellent for all algorithms and features considered. Pattern analysis allowed the best diagnostic performance (positive likelihood ratio: 5.1), whereas alternative algorithms revealed comparable sensitivity but less specificity. Interobserver agreement on management decisions made by dermoscopy was fairly good (mean kappa value: 0.53). The virtual Consensus Net Meeting on Dermoscopy represents a valid tool for better standardization of the dermoscopic terminology and, moreover, opens up a new territory for diagnosing and managing pigmented skin lesions.
Gossypol, a polyphenolic compound which depletes cellular energy by inhibition of several intrace... more Gossypol, a polyphenolic compound which depletes cellular energy by inhibition of several intracellular dehydrogenases, has been shown to have antiproliferative activity against human glial tumor cell lines in vitro and in nude mouse xenografts. Human trials of gossypol as a male contraceptive have demonstrated safety of long-term administration. We studied the activity of Gossypol 10 mg PO bid in 27 patients with pathologically confirmed glial tumors which had recurred after radiation therapy. Fifteen patients had glioblastoma, 11 patients anaplastic astrocytoma, 1 patient relapsed low grade glioma. Response was assessed every 8 weeks using CT/MRI scan and clinical criteria including decadron requirement. Treatment was continued until disease progression. Two patients had partial response (PR); 4 had stable disease for 8 weeks or more. One patient maintained a PR with improved KPS for 78 weeks. The other had a PR lasting 8 weeks. Toxicity was mild: 2 heavily pretreated patients had mild thrombocytopenia, 5 patients developed hypokalemia, 3 patients developed grade 2 hepatic toxicity and peripheral edema. Gossypol levels measured by HPLC did not correlate with response or toxicity in this study. We conclude that gossypol is well tolerated and has a low, but measurable, response rate in a heavily pretreated, poor-prognosis group of patients with recurrent glioma. The presumed novel mechanism of action, lack of significant myelosuppression, and activity in patients with advance glioma support further study of gossypol as an antineoplastic agent.
It has been proposed that preeclampsia is a metabolic syndrome of pregnancy. The polymorphisms Ps... more It has been proposed that preeclampsia is a metabolic syndrome of pregnancy. The polymorphisms PstI and MaeIII of INS, NsiI of INSR and Ala513Pro and Gly972Arg of IRS1 have been associated with metabolic syndrome; moreover, the products of these genes are functionally contiguous during insulin signaling. The aim of this study was to assess whether these polymorphisms are associated with preeclampsia. 46 normotensive pregnant women and 43 preeclamptic patients were included in the study to develop a clinical, biochemical and genotypic profile of preeclampsia. Clinical evaluation consisted of measurement of blood pressure, height and weight. Peripheral blood samples were collected for determination of fasting glucose and insulin concentrations and for extraction of genomic DNA. Proteinuria was determined. Polymorphisms were detected using PCR-RFLP. The normotensive and preeclampsia groups did not differ significantly in clinical and biochemical traits, except for systolic and diastolic blood pressure (p < 0.0001). Polymorphisms previously associated with metabolic syndrome in Mexican populations were not associated with preeclampsia in Mexican women (p > 0.05). The lack of an association between preeclampsia and the polymorphisms studied suggests that other genes whose products do not have direct functional interaction with metabolic syndrome or epigenetic factors may play a role in preeclampsia.
Tree row inventories are of increasing interest because tree rows mitigate wind erosion and deser... more Tree row inventories are of increasing interest because tree rows mitigate wind erosion and desertification, protect agricultural crops, enhance rural landscape quality, act as bio-corridors, carbon sinks, and a source for bio-energy. The main objective of tree row inventories is to estimate population parameters such as total tree numbers, total tree numbers by species, the mean stem diameter at breast height, the mean tree height and total wood volume. The estimation of these quantities may be straightforwardly carried out whenever aerial images are available in such a way that tree rows can be counted: in these cases, a two-stage cluster sampling may be performed in which the primary units sampled in the first stage are the tree rows in the study area while the secondary units sampled in the second stage are the trees within the selected rows. This paper proposes two sets of two-stage estimators for the interest parameters, based on the Horvitz–Thompson and ratio criteria, together with the corresponding estimators for their sampling variances. The use of stratification is also considered. The proposed procedure was applied to perform a tree row inventory in the Pontina plain (Central Italy): in this case, the tree rows were enumerated by means of ortho-corrected airborne images and stratification was carried out on the basis of the prevailing species and age classes. The inventory results are interesting from a forestry perspective as well as for checking the effectiveness of the procedure.
To assess bone regeneration in critical sized defects in the rabbit calvarium, filled with the bo... more To assess bone regeneration in critical sized defects in the rabbit calvarium, filled with the bone substitute calcium phosphate cement. Circular bone defects (8mm) were made in both parietal bones of 10 rabbits. One of the defects was filled with the calcium phosphate cement, and the other received autogenous bone harvested from the calvaria. The animals were killed at 3 or 6 weeks (n=5). Data analysis included qualitative assessment of the calvarial specimens and histomorphometric analysis was used to quantify the amount of new bone within the defects. The microscopic analysis of the samples showed bone healing with both calcium phosphate cement and autogenous bone graft. Data obtained from the histomorphometric analysis were statistically analyzed using 2-way analysis of variance and the Tukey's test. Data analysis showed that the autogenous bone graft had significantly more new bone compared with calcium phosphate cement at 3 and 6 weeks. Calcium phosphate cement at 6 weeks presented similar results to autogenous bone at 3 weeks. Both treatments presented an increase in bone healing with time. Treatments allowed bone regeneration that increased with time, however surgical cavities treated with the autogenous graft had more bone formation than those with calcium phosphate cement.
... Publishing Operations Division Director: Vanessa Hayden Directors: Karen Adams-Taylor, Susana... more ... Publishing Operations Division Director: Vanessa Hayden Directors: Karen Adams-Taylor, Susana Eloy de Vitale, Ryan Patella, Sue Sherrill Managers: David Antos, Karen Branham, Suzanne J. Bukovsky, Debbie Camp, Susan Chiara, Diane Darnell, Karl Elvin, Mary Ellen ...
Urban areas are continuously expanding today, extending their influence on an increasingly large ... more Urban areas are continuously expanding today, extending their influence on an increasingly large proportion of woods and trees located in or nearby urban and urbanizing areas, the so-called urban forests. Although these forests have the potential for significantly improving the quality the urban environment and the well-being of the urban population, data to quantify the extent and characteristics of urban forests are still lacking or fragmentary on a large scale. In this regard, an expansion of the domain of multipurpose forest inventories like National Forest Inventories (NFIs) towards urban forests would be required. To this end, it would be convenient to exploit the same sampling scheme applied in NFIs to assess the basic features of urban forests. This paper considers approximately unbiased estimators of abundance and coverage of urban forests, together with estimators of the corresponding variances, which can be achieved from the first phase of most large-scale forest inventories. A simulation study is carried out in order to check the performance of the considered estimators under various situations involving the spatial distribution of the urban forests over the study area. An application is worked out on the data from the Italian NFI.
Canadian Journal of Forest Research-revue Canadienne De Recherche Forestiere, 2008
... Area-based lidar-assisted estimation of forest standing volume Piermaria Corona and Lorenzo F... more ... Area-based lidar-assisted estimation of forest standing volume Piermaria Corona and Lorenzo Fattorini ... P. Corona.1 Dipartimento di Scienze dell'Ambiente Forestale e delle sue Risorse, Universita` della Tuscia, via San Camillo de Lellis, 01100 Viterbo, Italy. L. Fattorini. ...
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2000
Helminths, particularly some Schistosoma species, have been associated with cancer in humans. Neu... more Helminths, particularly some Schistosoma species, have been associated with cancer in humans. Neurocysticercosis, produced by cysticerci of the helminth Taenia solium, has been associated with the emergence of brain tumours and haematological malignancies. Local tumours, such as glioblastoma, could be explained by the induction of DNA damage in cells surrounding the cysticercus and chronically exposed to an inflammatory host response. However, systemic effects such as haematological malignancies are not easy to understand. The present work was conducted in Mexico to find out whether DNA damage arises in peripheral lymphocytes in patients with neurocysticercosis. We utilized a highly sensitive technique to analyse chromosomal aberrations, in-situ hybridization with probes against chromosomes 1, 2 and 4, and in addition the blocked-cytokinesis technique was used to determine the formation of micronuclei, a peculiar form of DNA damage. The study was made in lymphocytes from 8 patients before and after the administration of praziquantel, 1 of the 2 drugs used for neurocysticercosis treatment. The frequencies of chromosome aberrations and micronuclei in peripheral blood lymphocytes were higher in the infected patients as compared to those observed both in healthy donors and in the group of patients after praziquantel therapy. Our results suggest that chromosome aberrations induced in peripheral cells during neurocysticercosis could be associated with the development of haematological neoplasias.
Forest surveys performed over a large scale (e.g. national inventories) involve several phases of... more Forest surveys performed over a large scale (e.g. national inventories) involve several phases of sampling. The first phase is usually performed by means of a systematic search of the study region, in which the region is partitioned into regular polygons of the same size and points are randomly or systematically selected, one per polygon. In most cases, first-phase points are selected and recognized in orthophotos or very high resolution satellite images available for the whole study area. Disregarding the subsequent phases, the first phase of sampling can be effectively adopted to select small woodlots and tree rows, in the sense that a unit is selected when at least one first-phase point falls within it. On the basis of such a scheme of sampling, approximately unbiased estimators of abundance, coverage and other physical attributes readily measurable from orthophotos (e.g. tree-row length) are proposed, together with estimators of the corresponding variances. A simulation study is performed in order to check the performance of the estimators under several distributions of units over the study area (random, clustered, spatially trended).
Forest area in the year 1990 is a figure of great interest under the Kyoto Protocol. This note is... more Forest area in the year 1990 is a figure of great interest under the Kyoto Protocol. This note is devoted to a scientific exercise for the probabilistic ex post assessment of such a figure in Italy. Estimation was performed by two-phase point sampling, which made use of historical remotely sensed imagery. In the first phase, a sample of 12 089 points was selected according to an unaligned systematic sampling and the selected points were classified in land-use categories by Landsat 5 TM imagery. In the second phase, a sample of 3000 points was selected by stratified sampling in which the strata were determined by the satellite classification and the selected points were classified by aerial photos, assumed as ground truth. A two-phase estimate of land-use coverage partitioning the Italian territory was obtained together with a conservative estimate of the sampling variance-covariance. The procedure has proved to be of relatively easy implementation and objective repeatability.
Journal of The American Academy of Dermatology, 2003
There is a need for better standardization of the dermoscopic terminology in assessing pigmented ... more There is a need for better standardization of the dermoscopic terminology in assessing pigmented skin lesions. The virtual Consensus Net Meeting on Dermoscopy was organized to investigate reproducibility and validity of the various features and diagnostic algorithms. Dermoscopic images of 108 lesions were evaluated via the Internet by 40 experienced dermoscopists using a 2-step diagnostic procedure. The first-step algorithm distinguished melanocytic versus nonmelanocytic lesions. The second step in the diagnostic procedure used 4 algorithms (pattern analysis, ABCD rule, Menzies method, and 7-point checklist) to distinguish melanoma versus benign melanocytic lesions. kappa Values, log odds ratios, sensitivity, specificity, and positive likelihood ratios were estimated for all diagnostic algorithms and dermoscopic features. Interobserver agreement was fair to good for all diagnostic methods, but it was poor for the majority of dermoscopic criteria. Intraobserver agreement was good to excellent for all algorithms and features considered. Pattern analysis allowed the best diagnostic performance (positive likelihood ratio: 5.1), whereas alternative algorithms revealed comparable sensitivity but less specificity. Interobserver agreement on management decisions made by dermoscopy was fairly good (mean kappa value: 0.53). The virtual Consensus Net Meeting on Dermoscopy represents a valid tool for better standardization of the dermoscopic terminology and, moreover, opens up a new territory for diagnosing and managing pigmented skin lesions.
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